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Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... in HBSS (Gibco, #14175-129) at 37°C for 30min or 60min for stroma and epithelial layer ...
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bioRxiv - Cell Biology 2024Quote: ... #2028-EG-200) and Matrigel (Fisher Scientific, #356231) at a 30/70 ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... and MOLM-13 were cultured in RPMI (Gibco) with 20% FBS (Omega Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100ug/ml penicillin-streptomycin (Gibco). BT-474 and T-47D were generously gifted by Dr ...
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bioRxiv - Cancer Biology 2024Quote: ... while cDNA was generated using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... CM-H2DCFDA (ThermoFisher Scientific, C6827, Waltham, MA, USA), for 30 min to 1 h at room temperature according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... or with Lipofectamine 3000 (cat# L3000015, Invitrogen). For electroporation ...
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bioRxiv - Cancer Biology 2024Quote: ... Cre recombinase-mediated deletion of the K700E reporter was assessed on 2% agarose gel in 1xTAE buffer with a 1kB Plus Ladder (Invitrogen). RhAMP SNP primers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... All PCR products were resolved on 2% agarose gel in 1xTAE buffer with a 1kB Plus Ladder (Invitrogen). Primers to detect splice variants are listed in Supplementary Table 5.
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were maintained in RPMI 1640 media (Thermo Fisher, 11875093, Waltham, MA, USA), supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 5ng of total cDNA was used as inputs for qPCR on the QuantStudio 12K Flex (Applied Biosystems). To calculate relative ratio of splice variants ...
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bioRxiv - Cancer Biology 2024Quote: ... with 5% horse serum (Gibco/Life Technologies, cat#: 16050–130), 0.5 mg/ml hydrocortisone (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... with 10% Fetal Bovine Serum (Gibco, cat#: 16140089) and 1% Penicillin/Streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... or using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent (Fisher Scientific, AC158992500, Waltham, MA, USA) at 570 nm ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 mg/ml hydrocortisone (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells stably expressing a mitochondrial matrix-targeted GFP-aequorin (mt-AEQ) (Arduino et al, 2017) were cultured in DMEM supplemented with 10% FBS and 100 µg/mL geneticin (Thermo Fisher Scientific, 10131027). EFM19 cells (German Collection of Microorganisms and Cell Cultures GmbH ...
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bioRxiv - Cell Biology 2024Quote: ... CellTrace CFSE (Thermo Scientific) or CellTrace Far Red (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were: goat/donkey anti-mouse Alexa Fluor 488 1:500 (Molecular Probes), donkey anti-rabbit Rhodamine Red 1:500 (Jackson Immunireasearch) ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR was performed on a QuantStudio 7 Flex system (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... according to BCA quantification (Thermo Fisher Scientific, 23225) for each lane ...
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bioRxiv - Cell Biology 2024Quote: ... V5 (Thermo Fisher Scientific R96025), ACTIN (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... siRNAs to SRF were from Ambion (#13429) and GCGTGAAGA TCA AGATGGA obtained from Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... using TaqMan Universal Master Mix II with UNG (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using Trizol (Invitrogen, USA) according to the manual instruction ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 2.75 mM β-mercaptoethanol (Thermo Fisher Scientific, 21985-023) and denatured for 5 min at 90° C ...
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bioRxiv - Cell Biology 2024Quote: ... whereas MICU3 and EFHD1 EF-hand mutants were synthesized in the pUC57 vector (Thermo Fisher Scientific, SD0171) and cloned into a pDONR221 vector with the following primers ...
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bioRxiv - Cell Biology 2024Quote: ... protein samples were denatured at 70°C for 10 min in LDS Sample Buffer (Thermo Fisher Scientific, NP0007) with or without 100 mM Dithiothreitol (+DTT and –DTT ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended in ice-cold 1x BN Sample Buffer (Thermo Fisher Scientific, BN2003) with 1% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transiently transfected with mitochondrial matrix targeted RCaMP to measure changes in [Ca2+]mt then plated on Poly-D-Lysin coated 25 mm coverslips (Thermo Fisher Scientific, 25CIR-1.5). To measure changes in the [Ca2+]cyt cells were loaded with 2 μM Fura2AM (Moleculer probes ...
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bioRxiv - Cancer Biology 2024Quote: ... The concentration of tryptic peptides was measured using the Pierce™ Quantitative Peptide Assays kit (Thermo Fisher Scientific cat# 23275), and 25 µg of peptides were separated on a home-made reverse-phase C18 column in a pipet tip as previously (38) ...
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bioRxiv - Cancer Biology 2024Quote: ... mRNA expression was analyzed by QuantStudio 3 qRT-PCR System (Applied Biosystems, Fisher Scientific, Winnipeg, MB), and the Comparative CT (ΔΔCt ...
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bioRxiv - Cancer Biology 2024Quote: ... the top 35 strongest ions were selected and fragmented under direct control of Xcalibur software (Thermo Scientific). Parent MS spectra were acquired in the Orbitrap with a resolution of 120,000 ...
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bioRxiv - Cancer Biology 2024Quote: ... mRNA expression was analyzed by QuantStudio 3 qRT-PCR System (Applied Biosystems, Fisher Scientific, Winnipeg, MB), and the Comparative CT (ΔΔCt ...
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bioRxiv - Cancer Biology 2024Quote: ErbB3 silencing was achieved with 20nM of ErbB3 siRNA purchased from Ambion (Cat. AM16708, ID146247). The non-coding siRNA was acquired from Dharmacon (Horizon ...
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bioRxiv - Cell Biology 2024Quote: ... was washed and filled with 1x MOPS running buffer (Thermo Fisher Scientific, NP0001). Lanes were then fitted onto the 2D and overlaid with 1x SDS sample buffer ...
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bioRxiv - Cell Biology 2024Quote: ... the sample was centrifuged at 20000xg at 4°C for 30 min and the supernatant was transferred into a new pre-chilled tube with 0.25% G-250 (Thermo Fisher Scientific, BN2004). BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... A nanoLC-1000 (Thermo Scientific, USA) delivered a 75 min discontinuous gradient of 4 to 24 % of acetonitrile/0.1% formic acid at a flow rate of 800 nL/min ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was then performed using TaqMan Gene Expression Assays (Applied Biosystems, Foster City, CA, USA) (n=4) ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted from each well using 500µL Trizol followed by two sequential chloroform extractions followed by ethanol precipitation per the Trizol User Guide (Thermo Fisher #MAN0001271). Total RNA was depleted of rRNA using the Illumina rRNA Depletion Kit (NEB #E6310 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected using a transfection mix including Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific, 13778075), 0.5 µg of mt-AEQ (Rizzuto et al ...
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bioRxiv - Cancer Biology 2024Quote: ... An Orbitrap Fusion mass spectrometer (Thermo Scientific) was operated in data-dependent acquisition mode with the following parameters ...
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bioRxiv - Bioengineering 2024Quote: ... Digestion media consisted of DMEM (Gibco) supplemented with 0.5 mM CaCl2 and 2.5 mM MgCl2 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed in PBS and stained for 15 min on ice with 1:500 Live/Dead Fixable Violet Dye (Invitrogen) and 1:200 anti-mouse CD16/32 (clone 93 ...
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bioRxiv - Bioengineering 2024Quote: ... and Mycoplasma testing within 6 months with the MycoStrip Mycoplasma Detection Kit (Invitrogen, Waltham, MA). Cell lines were maintained in culture at 37°C in 5% CO2.
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bioRxiv - Bioengineering 2024Quote: ... 1:1 30% sucrose and OCT) before being embedded in OCT (Fisher Scientific). Frozen embedded samples were sectioned in 8 µm thickness until all three leaflets of the aortic root were visible ...
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bioRxiv - Bioengineering 2024Quote: ... The digested products (backbone of pcDNA™3.1 and HLA-E gene product) were ligated using the T4 DNA ligase enzyme (ThermoFisher, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Cell supernatants were collected and assayed using a TNFα ELISA (Invitrogen).
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bioRxiv - Bioengineering 2024Quote: ... cells were stained for 15 min on ice with 1:500 Live/Dead Fixable Violet Dye (Invitrogen) and 1:200 anti-mouse CD16/32 (clone 93 ...
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bioRxiv - Bioengineering 2024Quote: ... and normalized by total protein content from a Pierce BCA Protein Assay (Thermo Scientific).
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bioRxiv - Bioengineering 2024Quote: ... and assayed with an IL-10 ELISA (Invitrogen). IL-10 and Fab-IL-10 generated in our laboratories served as the standards for their respective ELISAs ...