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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... CRL-3216) were cultured at 37⁰C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% foetal calf serum (FCS ...
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bioRxiv - Microbiology 2024Quote: ... Cells were immobilised on 1% agarose pads (1.0 × 1.0-cm GeneFrames; Thermo Fisher Scientific). Cells were illuminated with 488 nm laser with 0.9% intensity and 150 ms exposure time and with 0.5% intensity and 100 ms exposure time for respectively RpsB-GFP and RpoC-GFP fusions ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed at least once in FACS staining buffer (ThermoFisher Scientific) to remove unbound antibody ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with O-propargyl-puromycin (OPP) using Click-iT® Plus OPP Alexa Fluor® 488 Protein Synthesis Assay Kit (Invitrogen) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... total protein was quantified using Pierce BCA Protein Assay Kit (ThermoFisher Scientific), and the PNGase F assay (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... using an Applied Biosystems Real Time PCR system (ThermoFisher Scientific). The primers used were Gag forward 5’-CTGTCGACGCAGTCGGCTTGCT- 3’ & reverse 5’-GCTCTCGCACCCATCTCTCTCCTTCTAGCC-3’ ...
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bioRxiv - Microbiology 2024Quote: ... An UltiMate 3000 nano-LC system was coupled to a Q Exactive HF-X mass spectrometer via an easy-spray source (all Thermo Fisher Scientific). The Q Exactive HF-X was operated in TMT mode with survey scans acquired at a resolution of 60,000 at m/z 200 ...
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bioRxiv - Microbiology 2024Quote: ... Chromatographic separation was performed on an Easy-Spray Acclaim PepMap column 50 cm long × 75 μm inner diameter (Thermo Fisher Scientific) at 55°C by applying 180 min acetonitrile gradients in 0.1% aqueous formic acid at a flow rate of 300 nl/min ...
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bioRxiv - Microbiology 2024Quote: ... or prepared using DMEM powder (Gibco) and adding 4 mM glutamine and 44 mM sodium bicarbonate ...
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bioRxiv - Microbiology 2024Quote: ... and imaged with an Invitrogen iBright FL1500 imaging system (ThermoFisher Scientific). Results were quantified by determining the area under peak for each band in ImageJ.
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bioRxiv - Microbiology 2024Quote: ... qPCR was completed using PowerUp SYBR Green Master Mix (ThermoFisher Scientific) and QuantStudio 3 Real Time PCR system.
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bioRxiv - Microbiology 2024Quote: ... Protein concentration of lysates was determined by Pierce BCA Assay (ThermoFisher Scientific). 15-20μg of total protein was loaded onto precast 4-12% iBlot2 Bis-Tris gels (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and M2 THP-1 and TLat 1D5 MDMs were differentiated as described above and seeded directly in a 6-well glass bottom plate (ThermoFisher Scientific) at a density of 0.6x106 cells/mL ...
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bioRxiv - Microbiology 2024Quote: ... and B-actin were detected by SYBR Green qRT-PCR (ThermoFisher Scientific) using an Applied Biosystems Real Time PCR system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... First strand cDNA synthesis was performed using the Verso cDNA synthesis kit (ThermoFisher Scientific). The expression levels of Gag ...
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bioRxiv - Microbiology 2024Quote: ... Cell viability was measured using CyQuant lactate dehydrogenase (LDH) cytotoxicity assay (ThermoFisher Scientific) in a 96-well plate.
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bioRxiv - Microbiology 2024Quote: ... PCR products were then cloned into pCR2.1 TOPO vector using the TOPO TA Cloning Kit (Invitrogen Thermo Scientific). From the pCR2.1 TOPO vector ...
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bioRxiv - Microbiology 2024Quote: ... The resulting cell population was stained with CD3 and CD14 antibodies (ThermoFisher Scientific) to determine purity and evaluated via flow cytometry (Fig ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were detected by horseradish peroxidase-conjugated secondary antibody using SuperSignal West Pico PLUS chemiluminescent substrate (ThermoFisher Scientific) and imaged with an Invitrogen iBright FL1500 imaging system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... cells were collected by trypsinization and DNA was isolated using Zymo Quick DNA mini-Prep kit (ThermoFisher Scientific). For viral particle genomes ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed three times with PBS for 10 min and shortly dipped in miliQ before being embedded on glass slides with ProLong glass antifade mountant (P36984, ThermoFisher). Coverslips were imaged using a Leica DM6B fluorescence microscope and analysed with the LASX software(Leica Microsystems ...
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bioRxiv - Microbiology 2024Quote: ... The kinetic measurements of sfGFP fluorescence in a cell-free reaction mix were performed in 384-well Greiner black plates by a Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific, US).
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bioRxiv - Microbiology 2024Quote: ... The gel was stained with SYBR Gold (Invitrogen) in 1xTBE for 15 min and visualized using a ChemiDoc (Bio-Rad).
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bioRxiv - Microbiology 2024Quote: ... 0.2 μL of RiboLock (ThermoFisher), 0.5 μL of the test compound ...
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bioRxiv - Microbiology 2024Quote: ... Each well was then imaged using the Evos FL Auto 2 cell imaging system microscope (ThermoFisher Scientific) and 25% field view from the centre of each well was captured using a 20× magnification lens.
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bioRxiv - Microbiology 2024Quote: ... USA) and stored at 4°C in Penicillin and Streptomycin (100 U/ml and 100 μg/ml, respectively; ThermoFisher Scientific #15140122) in Phosphate-Buffered Saline ...
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bioRxiv - Microbiology 2024Quote: ... The bacterial growth was assessed by scanning the absorbance at 620 nm using a spectrophotometer Multiscan GO (Thermo Scientific, Waltham, MA, US). The wells without visible bacterial growth were plated on MHB-agar and incubated ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells (ATCC, CRL-1586) and their derivatives were maintained in Dulbecco’s modified eagle medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2024Quote: ... paromomycin (Sapphire Bioscience #23634) were all reconstituted in 100% dimethyl sulfoxide (DMSO; ThermoFisher Scientific #4121). Reconstituted compounds were stored at -20°C for up to 3 months before use.
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bioRxiv - Microbiology 2024Quote: ... the primers were labeled with [γ-32P] ATP polynucleotide kinase (ThermoFisher, US) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Cells were uplifted gently using Accutase® (ThermoFisher, Cat. #A1110501) at RT for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... and genomic DNA was removed by Ambion Turbo DNA-free kit ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the DNA was estimated by utilizing the Qubit 4.0 fluorimeter manufactured (Thermo Fisher Scientific, Germany). The quality of the DNA was assessed using the NanoPhotometer N120 (Implen ...
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bioRxiv - Microbiology 2024Quote: ... and run on a precast 10% acrylamide TBE-Urea gel (Invitrogen) at 400 V using 1xTBE running buffer preheated to ∼50°C ...
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bioRxiv - Microbiology 2024Quote: ... HCT-8 cells were cultured in maintenance medium RPMI 1640 with GlutaMAX™ and HEPES supplements (ThermoFisher Scientific # 72400047) plus 5% foetal calf serum (FCS ...
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bioRxiv - Microbiology 2024Quote: ... with the addition of 1% GlutaMAX (Gibco, USA) and penicillin/streptomycin solution (100 units/mL and 100 μg/mL respectively ...
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bioRxiv - Microbiology 2024Quote: ... Germany).The sequence mixtures were prepared using a USB® Thermo Sequenase Cycle Sequencing Kit (Affymetrix, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... When cells reached 80-90% confluency they were passaged using 0.25% trypsin/EDTA (Gibco, Cat. #25200056).
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bioRxiv - Microbiology 2024Quote: ... The ermCL template for this experiment was obtained by PCR amplification using a Taq-DNA-polymerase kit (ThermoFisher, US), according to the standard protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell-free DNA was quantified via Qubit fluorometer using the dsDNA BR Assay Kit (Thermo Fisher Scientific). Additional size selection using Beckman Coulter beads was applied to remove high-molecular weight DNA reflective of cell-lysis and leukocyte contamination as previously described (38 ...
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bioRxiv - Molecular Biology 2024Quote: ... on a NanoDrop Microvolume Spectrophotometer (Fisher Scientific). 10 µg total protein per lane were resolved on a 10% SDS-PAGE gel.
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bioRxiv - Microbiology 2024Quote: The samples were observed using a Talos F200C G2 transmission electron microscope (Thermo Fisher Scientific, Waltham, MA, USA) at 200 kV with a 4k × 4k Ceta 16M CMOS detector (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... for 4 hrs while rotating at 4C then washed 3x with 200µL of lysis buffer using a DynaMag-2 magnet (Invitrogen #12321D). Bound protein was eluted with 45µL of 0.1-M Glycine-HCl buffer pH 3 and neutralized with 5µL of 0.5M Tris-HCl pH 7.4 1.5M NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... quantified using the Brachidonic-acid assay kit (BCA) (Thermo Fisher Scientific, Cat# 23227), separated on Mini-PROTEAN TGX gel (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... following the manufacturer’s instructions and quantified via the Qubit fluorometer dsDNA BR Assay Kit (Thermo Fisher Scientific). Genomic DNA was fragmented via sonication using a Covaris M220 instrument to the recommended 150-200 base pairs before library preparation ...
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bioRxiv - Cancer Biology 2024Quote: ... wild-type and point mutation constructs used for transient transfections were cloned in our laboratory into pcDNA3.1 (Thermo Fisher). TSC1 ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS; Thermo Fisher), HEK-293T cells were maintained at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded and transfected with 2.5 nM siRNA the next day using Lipofectamine2000 (Thermo Fisher) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Silencer Select siRNAs of Rheb (s12019, s12020, s12021), Rac1 (s11711, s11713), and Cdc42 (s2765, s2766, s2767) were purchased from Thermo Fisher. Dock7 shRNAs and negative control were purchased from Sigma (shRNA1 ...
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bioRxiv - Cancer Biology 2024Quote: ... then transfected with 4 μg DNA using Lipofectamine and Plus Reagent (Thermo Fisher) according to the manufacturer’s protocol ...