Labshake search
Citations for Thermo Fisher :
4601 - 4650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... 96-well Immuno 4 plates (Fisher Scientific) were coated with 10mg/ml goat anti-mouse Ig (Southern Biotechnology ...
-
bioRxiv - Immunology 2024Quote: ... Transfection was performed using Lipofectamine 3000 (ThermoFisher) in serum-free conditions ...
-
bioRxiv - Immunology 2024Quote: ... TCR or mock T-cells were labeled with 0.1 µg/mL Hoechst 33242 (Invitrogen) at 37°C/5%CO2 for 1h and 50,000 labelled T-cells were dispersed on top of the collagen gel ...
-
bioRxiv - Immunology 2024Quote: ... TCR T-cells were stained with cell tracker (CellTracker™ Green CMFDA Dye, Invitrogen), resuspended in T-cell medium and added on top of the 3D hydrogel at three different ratios of organoids to T-cells ...
-
bioRxiv - Cell Biology 2024Quote: ... the cells were incubated with Alexa Fluor 488-or Alexa Fluor 555-conjugated secondary antibody (Thermo Fisher Scientific) for 1 h at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... the coverslips were mounted with Prolong Diamond Antifade and DAPI (Thermo Fisher Scientific). After mounting medium is solidified ...
-
bioRxiv - Microbiology 2024Quote: ... and Pierce peptide desalting spin columns (Thermo Fisher Scientific, #89851), according to the manufactureŕs instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, Thermo Fisher Scientific) and separated on an in-house packed analytical column (35 cm x 75 μm ...
-
bioRxiv - Immunology 2024Quote: ... ∼15 to 30 mL ExpiCHO cell cultures were transfected using the Gibco ExpiCHO Expression System (ThermoFisher) as described by the vendor’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... Protein concentrations were determined using Pierce BCA Protein Assay Kit (Thermo Scientific) on a Benchmark Plus microplate reader (BIO-RAD) ...
-
bioRxiv - Microbiology 2024Quote: ... The TMT set was purified using a HiPPR detergent removal kit (Thermo Fisher Scientific, # 87780) and Pierce peptide desalting spin columns (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... or Alexa Fluor 647 were obtained from Life Technologies. The inhibitors dynasore or Y27632 were procured from Sigma-Aldrich.
-
bioRxiv - Immunology 2024Quote: ... Depleted sera were collected on the Kingfisher Flex extraction robot (ThermoFisher) and stored at 4°C.
-
bioRxiv - Microbiology 2024Quote: ... anti-occludin (331500; Invitrogen), and anti-tubulin (T5168 ...
-
bioRxiv - Microbiology 2024Quote: ... and peptides were labeled using TMTpro 18-plex isobaric mass tagging reagents (Thermo Fisher Scientific, #A52045) according to the manufactureŕs instructions ...
-
bioRxiv - Microbiology 2024Quote: The fractions were analyzed on an Orbitrap Fusion Lumos Tribrid mass spectrometer equipped with a FAIMS Pro ion mobility system and interfaced with an Easy-nLC1200 liquid chromatography system (all Thermo Fisher Scientific). Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm ...
-
bioRxiv - Immunology 2024Quote: ... or 0.1 μg/mL of CC40.8 or CV3-25) that were pre-labeled with Alexa Fluor 647 (using Zip Alexa Fluor™ Rapid Antibody Labeling Kit (ThermoFisher, Cat. #Z11235)) were added to the beads and incubated for 30 min at room temperature in dark ...
-
bioRxiv - Microbiology 2024Quote: ... were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 4.5 g of glucose per liter and 2 mM glutamine (catalog no. 11995-065; Life Technologies), supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Microbiology 2024Quote: Data analysis was performed using Proteome Discoverer version 3.0 (Thermo Fisher Scientific) and Sequest HT as a search engine ...
-
bioRxiv - Microbiology 2024Quote: ... Codon-optimized tandem copies of the sfmCherry gene fused to the pTAC promoter were synthesized by GeneArt (Thermo Fisher Scientific) and cloned into the HindIII and SpeI restriction sites of the pSEVA23a1 vector79 where a hygromycin resistance cassette had been inserted in BamHI and XbaI ...
-
bioRxiv - Cell Biology 2024Quote: ... for 30 min and stimulated with 10% fetal bovine serum (Thermo Fisher Scientific). For analysis of degradation pathways ...
-
bioRxiv - Cell Biology 2024Quote: ... signals were detected upon application of SuperSignal West Femto Maximum Sensitivity Substrat (Thermo Fisher Scientific). Image acquisition was performed with the ChemiDoc XRS+ (Bio-Rad ...
-
bioRxiv - Microbiology 2024Quote: THP-1 cells (ATCC) were maintained in RPMI 1640 containing GlutaMax (Thermo Fisher) and complemented with 10 mM Hepes (Thermo Fisher) ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were eluted in 30 μL nuclease-free water pre-warmed at 50°C and quantified by NanoDrop One (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2024Quote: ... samples were washed in PBS and a mixture of goat anti-Human Alexa488 conjugated secondary antibody (Thermo Fisher, diluted 1:2000) and DAPI (Thermo Fisher ...
-
bioRxiv - Microbiology 2024Quote: Expi293F cells (Thermo Fisher) were transiently transfected with plasmids carrying the heavy and the light chains of each antibody with a 1:2 weight/weight ratio ...
-
bioRxiv - Microbiology 2024Quote: ... using the Zip Alexa Fluor™ Rapid Antibody Labelling Kits (Thermo Fisher) following manufacturer instructions ...
-
bioRxiv - Microbiology 2024Quote: ... 2.5 μL 10X High Fidelity PCR Buffer (Invitrogen), 0.5 μL 10 mM dNTPs ...
-
bioRxiv - Microbiology 2024Quote: ... with an optimized cocktail of enhancers 1 and 2 (Thermo Fisher) added on day 1 post-transfection ...
-
bioRxiv - Molecular Biology 2024Quote: ... 30 μL of the cleared lysate was added to 400 μL TRI Reagent (Invitrogen, AM9738) for input RNA sequencing library ...
-
bioRxiv - Cell Biology 2024Quote: ... Gαs-KO cell line) were cultured in DMEM medium containing 4.5 g/l glucose (Gibco) supplemented with 10% fetal calf serum (FSC ...
-
bioRxiv - Cell Biology 2024Quote: ... 10 mM HEPES (Gibco), GlutaMax ...
-
bioRxiv - Cell Biology 2024Quote: ... the cells were washed 3-times with 1× PBS and stained with Alexa Fluor 568 goat anti-mouse IgG1 secondary antibody (Life Technologies, catalog # A21124) at room temperature for one hour ...
-
bioRxiv - Microbiology 2024Quote: ... was synthesized by GeneArt (Thermo Fisher Scientific) and cloned ...
-
bioRxiv - Microbiology 2024Quote: ... SDS-PAGE gel was transferred onto PVDF membrane using the iBlot™ Gel Transfer Device and Stacks (Thermo Fisher). Membranes were blocked for 1h at room temperature in TBS 1x/0.1% Tween-20/5% milk ...
-
bioRxiv - Microbiology 2024Quote: ... and complemented with 10 mM Hepes (Thermo Fisher), 1 mM Sodium Pyruvate (Thermo Fisher ...
-
bioRxiv - Microbiology 2024Quote: ... and DAPI (Thermo Fisher, diluted 1:2000) in PBS/BSA1% was added for 30 min at RT ...
-
bioRxiv - Microbiology 2024Quote: ... Fluorescence (λEx 560 nm and λEm 590 nm, exposure: 250 ms) was measured by the Varioskan Lux microplate reader (Thermo Fisher Scientific) upon 2 h incubation at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... and 10% fetal bovine serum (FBS) (Thermo Fisher). Three days before the assay ...
-
bioRxiv - Microbiology 2024Quote: ... Bacterial fluorescence was read using a Varioskan Lux microplate reader (Thermo Fisher) as a readout of the uptaken bacteria.
-
bioRxiv - Microbiology 2024Quote: ... parental plasmids were digested with DpnI (Thermo Fisher Scientific) and the linear PCR product was transformed into chemically competent MG1655 cells ...
-
bioRxiv - Microbiology 2024Quote: Bottom-up proteomic data analysis was performed using Proteome Discoverer (Ver. 3.0.1.27) (Thermo Fisher Scientific), and the Chimerys search algorithm ...
-
bioRxiv - Microbiology 2024Quote: ... cells were washed with phosphate-buffered saline 1X (PBS) and unspecific bindings were saturated with 50 µL of 20% normal rabbit serum (Life Technologies) in PBS ...
-
bioRxiv - Microbiology 2024Quote: ... adherent bacteria were fixed in 4% paraformaldehyde (PFA)/PBS (Thermo Fisher) or Cytofix (BD ...
-
bioRxiv - Microbiology 2024Quote: ... Data acquisition following separation was performed on a Q ExactiveTM Plus mass spectrometer (Thermo Fisher Scientific). A full scan MS acquisition was performed (350-1000 m/z ...
-
bioRxiv - Microbiology 2024Quote: ... 0.08 μL Platinum High Fidelity Taq (Invitrogen) and 1.0 μL cDNA ...
-
bioRxiv - Cell Biology 2024Quote: ... or 100 nM DAPI (Life Technologies). Coverslips were washed in 1× PBS and mounted in Aquamount (Andwin Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... mAb pool was dialyzed in PBS pH 7.4 using Slide-A-Lyzer G2 Dialysis Cassette 3.5K (Thermo Scientific) overnight at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... and 1% penicillin-streptomycin (ThermoFisher Scientific). All cell lines were maintained in a 37 °C incubator supplied with 5% CO2 ...
-
bioRxiv - Immunology 2024Quote: ... 1% GlutaMAX (ThermoFisher Scientific), and 1% penicillin-streptomycin (ThermoFisher Scientific) ...