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Citations for Thermo Fisher :
4651 - 4700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 900 x g for 5 minutes at room temperature and surface stained with Live/Dead Fixable Blue Dead Cell stain kit (Invitrogen) for 20 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2024Quote: ... The ViiA 7 Real-Time PCR System (ThermoFisher) was employed with the following cycling conditions ...
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bioRxiv - Immunology 2024Quote: ... according to the manufacturer’s protocol from Alexa Fluor®647 Labeling Kit (Microscale Protein Labeling Kit, cat. no. A30009; ThermoFisher Scientific) and stored at −20°C ...
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bioRxiv - Microbiology 2024Quote: ... was added to each well and luminescence was measured by the Varioskan Lux microplate reader (Thermo Fisher Scientific) with an exposure time of 500 ms ...
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bioRxiv - Microbiology 2024Quote: ... was added to each well and luminescence was measured by the Varioskan Lux microplate reader (Thermo Fisher Scientific) with 500 ms exposure ...
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bioRxiv - Microbiology 2024Quote: ... 0.25 µL of Superscript IV reverse transcriptase (Invitrogen) and nuclease-free water (DEPC ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM Sodium Pyruvate (Thermo Fisher) and 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... ultrapure BSA 1 mg/mL and DEPC H2O (Thermo Fisher Scientific) and stored at −80°C until use.
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bioRxiv - Microbiology 2024Quote: ... was used as soluble substrate and the final reaction was quantified at a wavelength of 405 nm by using the Varioskan Lux Reader (Thermo Fisher Scientific). After each incubation step ...
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bioRxiv - Microbiology 2024Quote: ... DNA was removed using the TURBO DNA-freeTM kit (Invitrogen) according to the ‘rigorous treatment’ instructions ...
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bioRxiv - Microbiology 2024Quote: ... Growth curves were recorded in 30-min time intervals with a cell density meter model 40 (Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... and pTRIP-SFFV-CD271-P2A-ACE2 (1.6 µg) in Opti-MEM (Gibco; 300 µL) containing X-tremeGene-9 (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... or 1:1000 goat anti-rabbit 647 (ThermoFisher, cat. #A-21245), and 1:1000 Hoechst dye (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: The coverslips were mounted onto slides (Fisher Scientific, cat. #1255015) with Invitrogen ProLong Gold Antifade Mountant (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: All chemicals were purchased from ThermoFisher (Waltham, MA) unless otherwise stated ...
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BTK and MMP9 regulate NLRP3 inflammasome-dependent cytokine and NET responses in primary neutrophilsbioRxiv - Immunology 2024Quote: ... Genotyping was performed using allele-specific Taqman® assays (Applied Biosystems, Thermo Fisher Scientific) on a QuantStudio 3 Real-time PCR equipment (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... IFI6 (ThermoFisher 4331182; Hs00242571_m1), OAS1 (ThermoFisher 4331182 ...
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bioRxiv - Immunology 2024Quote: ... the media was removed from four of the wells. They were then washed with 1x PBS (cat. 14190-144) and lysed with 1 mL TRIzol (Life Technologies, cat. 15596-018) for 5 minutes at room temperature before transferring to an Eppendorf tube and freezing at -80°C to await RNA extraction ...
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bioRxiv - Immunology 2024Quote: ... and SV40-Fribroblasts were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Fisher Scientific, cat. #11995065) supplemented with 0.1 mM nonessential amino acids (NEAA ...
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bioRxiv - Immunology 2024Quote: ... followed by cDNA synthesis using the SuperScript™ IV VILO™ Master Mix (Invitrogen, cat. 11756050) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and 1:400 dilution of Alexa488-conjugated anti-mouse antibody (Invitrogen). The cells were stained in 100 μL of diluted antibody per 106 cells for 1 hour at 4°C ...
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bioRxiv - Immunology 2024Quote: ... with Invitrogen ProLong Gold Antifade Mountant (Fisher Scientific, cat. # P36930). The slides were allowed to cure for 24 hours before the edges of the coverslips were sealed ...
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bioRxiv - Immunology 2024Quote: ... The cells were then washed twice with PBS-BGT before incubation with a secondary antibody solution of 1:1000 anti-mouse 588 (ThermoFisher, cat. #A-11001) and 1:1000 Hoechst dye (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... OAS1 (ThermoFisher 4331182; Hs00973635_m1). IFI6 and OAS1 were normalized to RPS11 mRNA levels using the deltaCt method [179].
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bioRxiv - Immunology 2024Quote: ... AR6 Pancytokeratin (1:200 MA513203, Invitrogen) Opal690 ...
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BTK and MMP9 regulate NLRP3 inflammasome-dependent cytokine and NET responses in primary neutrophilsbioRxiv - Immunology 2024Quote: ... on a QuantStudio 3 Real-time PCR equipment (Applied Biosystems, Thermo Fisher Scientific).
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BTK and MMP9 regulate NLRP3 inflammasome-dependent cytokine and NET responses in primary neutrophilsbioRxiv - Immunology 2024Quote: ... Then three washes were performed and 500 µL of Hoechst 33342 (Thermo Fisher; 2 μg/mL) were added for 5 min ...
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BTK and MMP9 regulate NLRP3 inflammasome-dependent cytokine and NET responses in primary neutrophilsbioRxiv - Immunology 2024Quote: ... For LDH assay the medium was replaced with OptiMEM (Thermofisher) without FCS prior to the second stimulation.
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bioRxiv - Immunology 2024Quote: ... 1X Antibiotic-Antimycotic solution (Gibco, cat. 15240-062), and 1X NEAA ...
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bioRxiv - Immunology 2024Quote: CLP was performed on WT and Plk3−/− mice following retro orbital injection (0.1μg/g mouse) of anti-mouse GPIX (Emfret) conjugated to AlexaFluor-750 (ThermoFisher) as previously described 21 ...
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bioRxiv - Cell Biology 2024Quote: ... and a Volta phase plate (VPP) (Thermo Fisher Scientific) for part of the data ...
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bioRxiv - Cell Biology 2024Quote: Tilt series for all the cells were collected on a Titan Krios (Thermo Fisher Scientific) operated at 300 kV equipped with a field-emission gun ...
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bioRxiv - Cell Biology 2024Quote: MT segmentations were performed with the automated filament tracing module in Amira software v.6.2.0 (Thermo Fisher Scientific) as described before (26) ...
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bioRxiv - Immunology 2024Quote: ... The precipitated DNA was resuspended in 20 µL of RNase-free H2O and in vitro transcribed with an SP6 mMessage mMachine In Vitro Transcription Kit (ThermoFisher, cat. AM1340). The generated RNA was electroporated into 1.2 × 107 BHK-21 cells ...
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bioRxiv - Immunology 2024Quote: ... the wells were washed with PBS and cells were harvested in 1 mL TRIzol (Invitrogen, cat. 15596-018). N = 3 replicates (separate wells ...
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bioRxiv - Immunology 2024Quote: ... 12mm glass coverslips (Fisher Scientific, cat. #1254581) placed at the bottom of the wells of a 24-well plate ...
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bioRxiv - Immunology 2024Quote: ... and 1:1000 Hoechst dye (ThermoFisher, cat. #62249) in PBS-BGT.
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BTK and MMP9 regulate NLRP3 inflammasome-dependent cytokine and NET responses in primary neutrophilsbioRxiv - Immunology 2024Quote: ... 100 μL/well anti-human MPO antibody (ThermoFisher) pre-diluted 1:500 in PBS was added in a black 96 well plate (Corning ...
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bioRxiv - Immunology 2024Quote: ... and the following assays: RPS11 (ThermoFisher 4331182; Hs01574200_gH), IFI6 (ThermoFisher 4331182 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were transfected with Lipofectamine MessengerMAX (Thermo Fisher, LMRNA008), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Dried coverslips were incubated in toluene (Fisher Scientific) with 12.5% v/v (3-mercaptopropyl ...
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bioRxiv - Cell Biology 2024Quote: ... and 10% defined fetal bovine serum (Thermofisher), referred to as full medium ...
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bioRxiv - Cell Biology 2024Quote: ... ECFCs were detached from culture dishes using TrypLE Express (Gibco) and used between passages 6 and 8.
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bioRxiv - Cell Biology 2024Quote: ECFCs were depleted of YAP and TAZ using custom siRNA (Dharmacon; Table 1) loaded lipofectamine RNAimax (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... capped RNA was generated from the pToto1101-nsP3-mScarletI plasmid (Invitrogen mMessage mMachine SP6 kit, AM1340) and electroporated into BHK-J cells ...
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bioRxiv - Immunology 2024Quote: ... a secondary antibody solution of goat anti-rabbit HRP (Invitrogen, cat. 31462) and visualized using a SuperSignal West Femto Maximum Sensitivity Substate kit (Thermo ...
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BTK and MMP9 regulate NLRP3 inflammasome-dependent cytokine and NET responses in primary neutrophilsbioRxiv - Immunology 2024Quote: ... on a QuantStudio 3 Real-time PCR equipment (Applied Biosystems, Thermo Fisher Scientific).
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BTK and MMP9 regulate NLRP3 inflammasome-dependent cytokine and NET responses in primary neutrophilsbioRxiv - Immunology 2024Quote: ... Genotyping was performed using allele-specific Taqman® assays (Applied Biosystems, Thermo Fisher Scientific) on a QuantStudio 3 Real-time PCR equipment (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... qPCRs were conducted on a QuantStudio 3 cycler using the Taqman Fast Advance master mix (Life Technologies Corporation, cat. 4444965) and the following assays ...
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bioRxiv - Immunology 2024Quote: ... and 1:1000 Hoechst dye (ThermoFisher, cat. #62249) in PBS-BG for two hours at room temperature ...