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Citations for Thermo Fisher :
4201 - 4250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and IGF-1 receptor (Catalog number: AM51331, siRNA ID:110754) with Lipofectamine RNAiMax Transfection Reagent (Invitrogen), according to manufacturer’s established protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Scrambled siRNA (Invitrogen; Catalog number: AM4613) was used as negative control (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... was used as negative control (Invitrogen) for transfection ...
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bioRxiv - Pathology 2024Quote: ... Recombinant seeds or insoluble fractions resuspended in PBS were added to the cells along with Lipofectamine 2000 transfection reagent (1uL lipofectamine per well; Thermo Fisher, 11668019). Cells were left to incubate and fixed after 72 hours ...
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bioRxiv - Pathology 2024Quote: ... followed by separating the left pulmonary lobe and freezing it in the RNAlater ® solution (Invitrogen™, USA) for a maximum of 30 days ...
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bioRxiv - Pathology 2024Quote: ... and scrambled negative control transfection (4611) were purchased from Life Technologies and used in accordance with the manufacturer’s guidelines ...
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bioRxiv - Pathology 2024Quote: ... and β-galactosidase (β-gal) plasmid using Lipofectamine 3000 Reagent (L3000015, Thermo Fisher). A total of 1200 ng of plasmid DNA was consistently transfected ...
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bioRxiv - Pathology 2024Quote: ... The quantitative polymerase chain reaction (qPCR) analysis was performed using the StepOne Plus® Real-Time PCR (Applied Biosystems, Foster City, CA, USA). Amplification was performed in a total volume of 25 µL/reaction ...
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bioRxiv - Pathology 2024Quote: Trizol (Invitrogen, California, USA) was used to extract total RNA ...
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bioRxiv - Pathology 2024Quote: ... This created a tet-on expression system in which TDP-43ΔNLS was expressed under the ACTA1 skeletal muscle promoter only when mice received a doxycycline (dox) chow diet (200 mg/kg, Fisher Scientific, 14-727-450). As controls ...
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bioRxiv - Pathology 2024Quote: ... Kidneys were divided longitudinally with one half stored in formalin for histological evaluation and one half placed in RNALater (Thermofisher Scientific). Sham animals underwent a midline laparotomy alone with no renal pedicle clamping ...
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bioRxiv - Pathology 2024Quote: ... The SYBR Select Master Mix (#4472903; Applied Biosystems) was used ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3 times with PBS, followed by incubation in secondary antibodies (goat antibodies conjugated with Alexa Fluor 488, 546 or 647 (Invitrogen)) for 1 hour at room temperature ...
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bioRxiv - Pathology 2024Quote: ... qRT-PCR was performed using an ABI PRISM 7700 Sequence Detection System (Applied Biosystems, Foster City, CA, USA). The SYBR Select Master Mix (#4472903 ...
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bioRxiv - Pathology 2024Quote: ... Samples were analyzed on a ViiA 7 Real-Time qPCR System (Thermo Fisher Scientific), and relative expression levels were normalized to a standard reference gene (18s rRNA ...
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bioRxiv - Pathology 2024Quote: ... quantified by NanoDrop spectrophotometer and used to prepare cDNA with Multiscribe reverse transcriptase (Thermo Fisher Scientific). Quantitative PCR was performed using Power SYBR Green or Taqman PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: Small interfering RNA (siRNA) targeting NFIX and negative control oligonucleotides were purchased from Invitrogen (#1299003, Carlsbad, CA, USA). Three independent NFIX-specific siRNAs were used in this study ...
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bioRxiv - Pathology 2024Quote: ... with a biotin conjugate was used (1:200) followed by AB reagent or streptavidin conjugated with AlexaFlour 488/594 fluorophore (ThermoFisher Scientific). For IF detection ...
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bioRxiv - Pathology 2024Quote: ... linear ion trap mass analyzer (Q-OT-qIT, Thermo Scientific). Tandem MS was performed by isolation at 1.5 Th with the quadrupole ...
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bioRxiv - Cell Biology 2024Quote: ... with PowerUp SYBR Green Master Mix (Applied Biosystems). All primer sets are as listed in Table S2.
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bioRxiv - Cell Biology 2024Quote: ... qPCR was run on an Applied Biosystems QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific) with PowerUp SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... 2mM EDTA (Invitrogen), and 2% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... The RNA concentration was measured by NanoDrop 8000 Spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... Dynabeads mRNA DIRECT™ kit (Thermo Fisher Scientific) was used to extract polyadenylated RNA (polyA RNA ...
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bioRxiv - Cell Biology 2024Quote: Knockdown of SON and SRRM2 in HeLa cells was performed using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific). siRNAs were designed and purchased from IDT ...
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bioRxiv - Cell Biology 2024Quote: ... cells were digested with proteinase K (Thermo Fisher Scientific), and the nucleic acids ...
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bioRxiv - Cell Biology 2024Quote: Total RNA from nuclei or whole cells was purified with TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 50 U/mL penicillin/streptomycin solution (Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... 0.05 mM dTTP (Thermo Fisher Scientific), 0.05 mM dCTP (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 mM dGTP (Thermo Fisher Scientific), 1 U/μL RNaseOUT (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and 10% fetal bovine serum (FBS, Gibco). Mycoplasma contamination was regularly tested for both cell lines ...
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bioRxiv - Cell Biology 2024Quote: ... 1 U/μL RNaseOUT (Thermo Fisher Scientific) in 50 μL buffer of DPBS supplemented with 3 mM MgCl2 ...
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bioRxiv - Pathology 2024Quote: ... A final concentration of 30 nM of each siRNA was transfected into cultured cells using Lipofectamine 2000 (Life Technologies), as described previously23.
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bioRxiv - Pathology 2024Quote: ... Transfection of GC cell lines was conducted using Lipofectamine RNAiMAX (#13778075, Invitrogen, Waltham, Massachusetts, USA) following previously described methods (25) ...
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bioRxiv - Pathology 2024Quote: ... was maintained at 37°C in Dulbecco’s modified Eagle’s medium (DMEM-F12) (#11320033, Thermo Fisher Scientific) containing 10% fetal bovine serum (#35-015-CV ...
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bioRxiv - Pathology 2024Quote: ... AlexaFluor 488 goat anti-mouse IgG (H+L) and Alexa Fluor 594 goat anti-mouse IgG (H+L) secondary antibodies were used at 1:500 dilution (ThermoFisher Scientific). DAPI (4’,6-diamidino-2-phenylindole ...
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bioRxiv - Pathology 2024Quote: ... Immobilized antibodies were detected by UltraVision-LP HRP Polymer detection system specific for anti-mouse and anti-rabbit IgG (ThermoFisher Scientific), or with biotinylated secondary antibodies and AB reagents (Vector Laboratories ...
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bioRxiv - Pathology 2024Quote: ... Primers for Sh3bgr cryptic exon detection (Table 4) were used to amplify the targets using the DreamTaq 2x master mix (Thermo Fisher, K1081). The PCR reaction settings using a Bio-Rad C1000 Touch Thermal Cycler were 95 °C for 60s ...
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bioRxiv - Paleontology 2024Quote: ... The data were then processed using Geomagic Wrap (3D Systems, Rockhill) and photographs of the reconstructed models acquired with Avizo v.7.0 (Thermo Fisher Scientific, Waltham).
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bioRxiv - Cell Biology 2024Quote: ... by iBlot (Invitrogen). Membranes were incubated with rabbit anti-NF-κB p65 antibody [E379] (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... the cell mass was normalized quantifieng total protein using the BCA protein assay kit (ThermoFisher, Cat# 23225).
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bioRxiv - Cell Biology 2024Quote: ... the ABI PRISM 7000 Sequence Detector (Applied Biosystems) was used ...
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bioRxiv - Cell Biology 2024Quote: ... DNA was purified using a QIAquick PCR Purification kit and qPCR was performed with Power Up SyBr Green PCR master mix using the ABI 7900HT Real Time PCR system (Applied Biosystems, Waltham, MA). PCR reactions contained primers for murine Vcan Prom400 (mVCAN Prom 400 ...
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bioRxiv - Cell Biology 2024Quote: ... and normalized to protein levels determined in the same lysate by Pierce BCA Protein Assay Kit (Thermo Scientific, USA).
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bioRxiv - Cancer Biology 2024Quote: ... collagenase type 4 (Fisher Scientific), and dispase (Corning) ...
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bioRxiv - Cell Biology 2024Quote: ... Halt proteases with phosphatase inhibitors and high-capacity cDNA archive kits were from ThermoFisher Scientific (Grand Island ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were decalcified in 0.5M EDTA at pH8 (Invitrogen, 15575-038) for 8 days ...
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bioRxiv - Cell Biology 2024Quote: ... 1% penicillin-streptomycin (Gibco), 0.02 mM L-ascorbic acid (Sigma ...
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bioRxiv - Neuroscience 2024Quote: All samples (∼ 1 µg for each fraction) were loaded and eluted by an Ultimate 3000 RSLCnano (Thermo Scientific) with an in-house packed 20 cm ...
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bioRxiv - Neuroscience 2024Quote: The PCR reaction was performed using TaqMan Fast Universal PCR Master Mix (#4367846, Foster City, CA, Applied Biosystems by Life Technologies) and MicroAmp Fast Optical 96-Well reaction plates (#4346906 ...