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Citations for Thermo Fisher :
4001 - 4050 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Resulting tryptic peptides were separated by reverse-phase high-performance liquid chromatography (RP-HPLC; UltimateTM 3000 RSLCnano system; Thermo Scientific) using a 50 cm analytical column (in-house packed with Poroshell 120 EC-C18 ...
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bioRxiv - Physiology 2023Quote: ... 1 µg peptides were injected on an Ultimate 3500 RSLC coupled to a Q-Exactive HF-X (Thermo Fisher Scientific). Peptides were first trapped using a PepMap 100 C18 trap column and then separated with a PepMap C18 analytical column (75 µm x 50 cm ...
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bioRxiv - Physiology 2023Quote: ... with peptides introduced via an EasySpray Nano source coupled to a Q-Exactive Plus Quadrupole Orbitrap mass spectrometer (Thermo Scientific) and subjected to data dependent tandem mass spectrometry (MS/MS) ...
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bioRxiv - Systems Biology 2024Quote: ... Peptides was were loaded via an Acclaim PepMap 100 trap column (C18, 5 μm, 100Å; Thermo Scientific, San Jose, CA) onto a Waters BEH C18 column (100 µm × 100 mm ...
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bioRxiv - Molecular Biology 2023Quote: ... Tryptic peptides were analyzed with a Vanquish Neo UHPLC system coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific). Peptides were separated using an increasing acetonitrile gradient from 2% to 25% over 80 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluting peptides were ionized at a potential of + 2 kVa into a Q Exactive mass spectrometer (Thermo Fisher, Germany). Intact masses were measured at resolution 70.000 (at m/z 200 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was transferred to a new tube and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84688) following manufacturer’s recommendation.
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bioRxiv - Biochemistry 2023Quote: ... peptides were loaded with autosampler and trapped in an Acclaim PepMap 100 trap column (75µm ξ 2 cm, Thermo Scientific) at 4 µL/min with 100% solvent A (0.1% formic acid in water ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped on a PepMap C18 trap column (300 µm x 5 mm, 5 µm particle size, Thermo Fisher) and separated on a 50 cm Easy-Spray column (ES803 ...
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bioRxiv - Neuroscience 2023Quote: The peptides were analysed using liquid chromatography/mass spectrometry (MS) using with an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) coupled to an UltiMate3000 RSLCnano LC system (Dionex Co. ...
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bioRxiv - Developmental Biology 2023Quote: ... Final peptide concentration was measured by absorbance at 280 nm on a Nanodrop ND-2000 spectrometer (Thermo Fisher, Waltham, MA).
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bioRxiv - Bioengineering 2024Quote: ... quenchbodies were eluted from the beads using 50 μL of 1.5 mg/mL Pierce™ 3 x DYKDDDDK Peptide (ThermoFisher).
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bioRxiv - Biochemistry 2024Quote: ... Peptide mixtures of three independent replicates per protein complex were analyzed on an LTQ-Orbitrap XL (Thermo Scientific, Bremen, Germany), which was directly coupled to an UltiMateTM 3000 HPLC or RSLCnano system (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides eluted from the column were analyzed using a Q Exactive HF-X or Orbitrap Exploris 480 (Thermo Fisher Scientific) for overlapping window DIA-MS (20 ...
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bioRxiv - Immunology 2023Quote: Sorted lung DC1 were loaded with 10 nM MHC class I-restricted OVA peptide (OVA257-264, SIINFEKL) in complete IMDM media (Gibco) for 1.5 hours at 37°C in a Eppendorf tube ...
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bioRxiv - Biochemistry 2023Quote: ... We analyzed MS/MS data collected from ND samples to identify the likely sequence of the patent peptides using SEQUEST (Bioworks v3.3.1, Thermo Fisher Scientific) with a peptide tolerance of 8 ppm and a fragment tolerance of 0.1 AMU ...
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bioRxiv - Microbiology 2024Quote: Peptide mixtures were analyzed using two-dimensional (2D) liquid chromatography (LC) on an Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) coupled with a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA encoding the mature protein (without the signal peptide) was amplified using gene-specific primers incorporating restriction enzyme sites and subcloned into the pPICZα A vector (Invitrogen) using EcoRI and XbaI enzymes and T4 ligase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were digested into peptides by adding 0.95 µg of MS grade trypsin (Thermo Scientific Pierce, Rockford, IL, USA) solubilized in 40 µl of ABC buffer to the filters and incubating for 16 hours in a wet chamber at 37 °C ...
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bioRxiv - Physiology 2024Quote: ... and 10 μg of peptide per sample was labeled with TMTpro18 plex reagents (Cat. # A52045; Thermo Fisher Scientific, Darmstadt, Germany). TMT-labeled samples were adjusted to equal amounts and pooled ...
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bioRxiv - Cell Biology 2024Quote: ... The digested peptides were then desalted using Pierce C18 Spin Tips & Columns system according to the manufacturer’s instructions (ThermoFisher Scientific). Three independent biological replicates for each condition were analyzed by mass spectrometry.
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bioRxiv - Biochemistry 2024Quote: ... Peptide separation was performed on a EASYspray C18 column (50 cm x 75 um, Thermo Fisher Scientific, San Jose, CA) over a 65-minute gradient at a flow rate of 250 nL/min ...
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bioRxiv - Cancer Biology 2024Quote: ... peptides were separated on a 75 µm ID x 25 cm C18 Acclaim PepMap® RSLC column (Thermo Scientific, USA) with a 4-40% linear gradient of solvent B (0.1% formic acid in 80% ACN ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were first trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Cancer Biology 2024Quote: T cells from KOG mouse TDLNs (5 × 105) were co-cultured with SIINFEKL peptide (1 μg/mL, Thermo Fisher Scientific) in a 96-well plate (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... and the resulting peptides were analysed by LC-MSMS on an Orbitrap Fusion™ Lumos™ mass spectrometer (ThermoFisher Scientific) using CID+ETD activation ...
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bioRxiv - Biochemistry 2024Quote: Enriched crosslinked peptides were injected onto a C18 PepMap100-trapping column (0.3 x 5 mm, 5 μm, Thermo Scientific™) connected to an in-house packed C18 analytical column (75 μm x 300 mm ...
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bioRxiv - Biochemistry 2024Quote: The tryptic peptides were injected onto a pre-column (PepMap C18, 5 mm × 300 μm × 5 μm, Thermo Fisher Scientific) and separated ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting peptides were desalted using Sep-Pak C18 cartridges (Waters, MA, USA) and dried in a SpeedVac (Thermo Scientific).
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bioRxiv - Biochemistry 2024Quote: ... Peptides were first loaded onto an Acclaim PepMap C18 nano-trap column (100Å, 3µm, 75µm x 2cm, Thermo Scientific 164946) in mobile phase A ...
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bioRxiv - Biochemistry 2024Quote: ... The digestion was quenched in 0.5% trifluoroacetic acid and spiked with Pierce Retention Time Calibrant peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/μL ...
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bioRxiv - Molecular Biology 2024Quote: The resulting peptide mixtures were analyzed in data-dependent acquisition mode with an Orbitrap Exploris high-resolution mass spectrometer (MS) (ThermoFisher) coupled to an UltiMate 3000 LC system (Dionex-LC Packings) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 µg of peptides for each sample were labeled with 50 µg of TMTpro16-plex reagents (Thermo Fisher Scientific) per channel ...
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bioRxiv - Cancer Biology 2024Quote: The peptide samples (1-1.5 μL) were analyzed with a Q-Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) equipped with a nano-electrospray source operating at 2 kV at a capillary temperature of 320 °C and coupled to an EASY-nLC 1200 UHPLC system (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... 4 µg of peptides were analyzed in a QExactive mass spectrometer coupled to a nano EasyLC 1000 (Thermo Fisher Scientific). Solvent composition at the two channels was 0.1% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were incubated for 1 hour at room temperature in blocking buffer containing the following secondary antibodies: Goat anti-Rabbit Alexa Fluor® 594 conjugate (1:250, #A-11037, Life Technologies) and Donkey anti-Mouse Alexa Fluor® 488 conjugate (1:250 ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were permeabilized with 0.5% Triton X-100 in DPBS for 10 minutes and incubated in BlockAid Blocking Solution (Thermo Fisher, Cat# B10710) for 1 h at RT in a humidity chamber and incubated O/N in primary antibody ...
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bioRxiv - Cell Biology 2020Quote: ... NRK cells expressing WT importin α or mutant importin α S62A released from thymidine blocking were changed to CO2-independent medium (Invitrogen), 10% cosmic calf serum (HyClone) ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody was diluted to 1:3000 in blocking buffer and added to the plates for 60 minutes followed by TMB substrate (Thermo Fisher Scientific). Upon stopping the reaction with 1 M H2SO4 ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were incubated with the following primary antibodies in blocking buffer overnight at 4 °C: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific) and mouse anti-OCT4 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Cryosections of the cochlea and brain slices were washed in PBS for 1 h and subsequently incubated in secondary antibody solution containing blocking solution and the secondary antibodies Alexa Fluor 488-conjugated goat anti-rabbit (1:1000, cochlear cryosections) (Molecular Probes (A11008)) ...
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bioRxiv - Developmental Biology 2021Quote: ... The samples were then washed three times with 1xPBS and incubated with Alexa 594 conjugated anti-mouse IgG antibody (1:500 in blocking buffer, ThermoFisher Scientific, A11012) and Alexa 488 conjugated anti-rabbit IgG antibody (1:500 in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for one hour on the rocker at room temperature in blocking solution with 1:1000 Goat anti-Rabbit IgG H&L Alexa Fluor 405 (Thermo Fisher Scientific). Then sections were washed once in PBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature for all target proteins or with clear milk blocking buffer for β-actin protein (Pierce, Thermo Scientific). We used the following LARP1 (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed 3×5mins in PBS and incubated for two hours in appropriate secondary antibodies (Invitrogen; 1:250 in blocking solution). For double labelling of Iba1 and GAD67 ...
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bioRxiv - Bioengineering 2019Quote: ... cardiomyocytes were incubated in blocking solution containing Alexa Fluor Plus 488-conjugated goat anti-mouse IgG (1:300, A32723; Thermo Fisher Scientific) for 60 min ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were rinsed three times with PBS and blocking buffer with AlexaFluor 488-coupled goat anti-mouse antibody (Life Technologies, 1:1000) and 0.8 μg/mL DAPI (4,6-diamidino-2-phenylindole ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were blocked in blocking buffer (1% BSA, PBST) for 1h at room temperature and incubated with primary antibody LC3A/B (Thermo Scientific, USA) overnight at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Slides were washed three times for 5 min in 1X PBS solution and then incubated for 3 h at room temperature in 100 μL blocking buffer containing Alexa 488-conjugated goat anti-rabbit secondary antibody (Molecular Probes, Invitrogen), diluted 1:200 in fresh blocking buffer ...