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Citations for Thermo Fisher :
3851 - 3900 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... sample aliquots containing 25 µg of dried peptides were subsequently labeled with isobaric tag (TMT 6-plex, Thermo Fisher Scientific) following a recently established protocol 45 ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were dissolved in 3% acetonitrile/0.1% formic acid and 200 ng of peptides were injected on an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) coupled to an UltiMate 3000 UHPLC (Thermo Scientific) ...
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bioRxiv - Immunology 2019Quote: ... The full length and truncation sequences were transferred into a Gateway compatible destination vector that includes an N terminal triple-epitope tag (S protein tag, FLAG epitope tag and Streptavidin-binding peptide tag) using the LR clonase enzyme kit (ThermoFisher). Plasmids were transfected into 293T cells using FuGENE transfection reagent (Promega ...
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bioRxiv - Plant Biology 2021Quote: 100 μg peptide mixture of each sample was labelled using TMT reagent according to the manufacturer’s instructions (Thermo Fisher Scientific) and analysed on an Orbitrap Fusion Lumos (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: A total of 100 µg peptides from each sample were labeled using the TMT11plex Mass Tag Label Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were eluted and ionized by electrospray followed by mass spectrometric analysis on an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher). MS data were acquired using the FTMS analyzer and MS/MS data using the ion trap analyzer ...
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bioRxiv - Biophysics 2019Quote: ... Cleavage of the signal peptide was confirmed by transfecting and expressing individual constructs and ensuring they were secreted from transfected Expi293 cells (ThermoFisher) and by glycosylation-corrected molecular weight determination using SEC-MALS (Wyatt Technology).
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bioRxiv - Neuroscience 2019Quote: ... RAI1 antibodies were generated by immunizing rabbits with a synthesized RAI1 peptide (aa 28 to 42, ENYRQPGQAGLSCDR, Thermo Fisher Scientific), followed by affinity purification using the peptide as the affinity ligand (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... the peptides were separated by reversed-phase high-performance liquid chromatography using an Easy-nLC 1000 Nanoliquid chromatography (ThermoFisher Scientific). The separation was carried out in a homemade column 25 cm in length and 100 μm in diameter packed with a C18 adsorbent ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were initially trapped in C18 PepMap100 pre-column (300 µm inner diameter x 5 mm, 100A, Thermo Fisher Scientific) in Solvent A (Formic acid 0.1% (v/v) ...
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bioRxiv - Neuroscience 2020Quote: ... biotinylated amyloid peptides spanning the amyloid beta sequence (−5 to 45) were pre-incubated with NeutrAvidin (ThermoFisher Scientific, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... In all cases peptides were separated using reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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bioRxiv - Neuroscience 2020Quote: ... we designed a QuasAr-P2A-GCaMP/QuasAr-P2A-vGlut-pHluorin by inserting QuasAr fused to P2A peptide synthesized using GeneArt Gene Synthesis (Invitrogen) and GCaMP/vGlut-pHluorin under the human Synapsin1 promoter ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were eluted with 0.1% (v/v) trifluoroacetic acid (TFA) and dried in a SpeedVac system (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The fractionated peptides were analysed by LC-MS/MS using a fully automated Ultimate 3000 RSLC nano System (Thermo Scientific) fitted with a 100 μm x 2 cm PepMap100 C18 nano trap column and a 75 μm × 25 cm reverse-phase NanoEase M/Z HSSC18 T3 column (Waters) ...
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bioRxiv - Immunology 2020Quote: ... with or without the presence of peptides or anti-CD3 and inhibitors in a final volume of 200 μl RPMI1640 + L-glutamine (Gibco) containing 10 % FCS and penicillin/streptomycin (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Immunology 2020Quote: ... The peptide samples derived from individual patients were then individually labelled using the TMT10plex mass tag kit (Thermo scientific, USA) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: Protein samples were injected for inline pepsin digestion and the resulting peptides were identified using tandem MS (MS/MS) with an Orbitrap mass spectrometer (Fusion Lumos, ThermoFisher). Following digestion ...
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bioRxiv - Biochemistry 2019Quote: ... A total of 100 μg peptides from each time course was labeled with iTRAQ™ (Applied Biosystems, Foster City, CA) as described by the manufacturer (0 hour/control ...
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bioRxiv - Cell Biology 2019Quote: ... Peptides were directly eluted (∼250nl/min) via a Triversa Nanomate nanospray source into a Orbitrap Fusion mass spectrometer (Thermo Scientific). Positive ion survey scans of peptide precursors from 375 to 1575 m/z were performed at 120 K resolution (at 200m/z ...
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bioRxiv - Cell Biology 2020Quote: ... peptides were first trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Biochemistry 2020Quote: ... The application of a 2.5 kV distal voltage electrosprayed the eluting peptides directly into LTQ linear ion trap mass spectrometers (Thermo Scientific) equipped with a custom-made nano-LC electrospray ionization source ...
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bioRxiv - Cancer Biology 2021Quote: ... The redissolved peptides of each sample were acquired and analyzed by EASY-nLC™ 1200 System (Thermo Fisher Scientific™) coupled to a QE HF-X mass spectrometer (Thermo Fisher Scientific™) ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 300 μl of Binding Buffer with 2 μg of biotin-labeled tubulin peptide (K40 only, K40-Ac and K40-me3) (ThermoFisher), and samples were rotated at 4 °C for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were first trapped onto a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide extracted from each set were pooled and analyzed by nanoLC-MS/MS using an Ultimate 3000 system (Thermo Scientific) coupled to a TripleTOFTM 6600 mass spectrometer (AB Sciex) ...
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bioRxiv - Biochemistry 2021Quote: LC-MS analyses of tryptic peptide mixtures were performed using an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) connected to an UltiMate 3000 RSLCnano HPLC system (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Synthetic peptides (60 nmol each) were incubated with 18 μL streptavidin agarose resins (high capacity streptavidin agarose, Thermo Fisher Scientific) per experiment for 2 hr at room temperature in 80 μL of lysis buffer (1% NP-40 ...
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bioRxiv - Biochemistry 2021Quote: ... Eluting peptides were electrosprayed into the mass spectrometers (Exactive Plus EMR-Orbritrap or Q-exactive HF, both Thermo Fisher Scientific). Data were recorded using Xcalibur software (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... peptides were reconstituted in 6 μL of loading buffer (2% ACN, 0.1% TFA) and peptide concentration was estimated via optical measurement at 280 nm (Nanodrop 2000; Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: Peptides were loaded directly onto a 75 μm x 50 cm PepMap-C18 EASY-Spray LC Column (Thermo Fisher Scientific) and eluted at 250 nl/min using 0.1% formic acid (Solvent A ...
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bioRxiv - Biochemistry 2020Quote: ... 1 nM of each acyl-peptide was added to 100 ng of a HeLa tryptic digest standard (Thermo Scientific, #PI88329) for a final ratio of 1 fmol acyl-peptide/100 ng of HeLa digest ...
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bioRxiv - Cell Biology 2021Quote: ... For peptide treatment, cells were washed with PBS and treated with peptides (40 μM, 1–4 h) dissolved in OPTI-MEM (Gibco) acidified with 0.15% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted peptides were separated using an Acclaim PepMap100 analytical column (2 μm, 50-cm by 75-μm ID; ThermoFisher Scientific). The 125-min elution gradient at a constant flow rate of 300 nl/min was set to 5% phase B (0.1% formic acid in 99.9% acetonitrile ...
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bioRxiv - Biophysics 2020Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The construct was transformed into bacterial DH10Bac component cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were eluted from the column and electrosprayed into a Velos Pro Linear Ion Trap Mass Spectrometer (Thermo Fisher Scientific). Data was acquired using data-dependent acquisition (DDA ...
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bioRxiv - Immunology 2020Quote: ... Peptide stimulated and non-stimulated cells were first labeled with live/dead detection kit (Thermo Fisher Scientific, Waltham, MA, USA) and then resuspended in BD Fc Block (clone 2.4G2 ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of peptides was injected onto a nanoscale C18 reverse-phase chromatography system (UltiMate 3000 RSLC nano, Thermo Scientific) and electrosprayed into an Orbitrap mass spectrometer (LTQ Orbitrap Velos Pro ...
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bioRxiv - Microbiology 2021Quote: ... Peptide identification of the human and SARS-CoV-2 proteome was performed by Proteome Discoverer (version 2.2, Thermo Fisher Scientific) using the SequestHT search engine as described (Seidel et al 2018) ...
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bioRxiv - Developmental Biology 2021Quote: ... The digested peptides were eluted on a capillary column and sprayed into a Q Exactive Mass Spectrometer (Thermo Fisher Scientific) equipped with a nano-ESI ion source (National institute of Bilogical Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then separated into eight fractions using the High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific Inc.). The fractions were dissolved in 0.1% formic acid ...
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bioRxiv - Immunology 2022Quote: The tryptic peptides were loaded onto an Acclaim PepMap 100 C18 trapping column(C18, 1.9 μm, 75 μm*20 cm; Thermo Fisher) at a flow rate of 200 nL/min ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were labeled in DMSO using ~1.3-fold molar excess of Oregon Green 488 maleimide (Life Technologies/Molecular Probes #O6034) dissolved in a 1:1 solution of acetonitrile:DMSO ...
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bioRxiv - Biochemistry 2022Quote: LC-MS/MS: Generated peptides were injected into a PepMap C18 5 mm x 300 um column (Thermo Fisher Scientific) at a flow of 25 μL/min with 0.1% TFA mobile phase ...
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bioRxiv - Cell Biology 2022Quote: ... LC-MS/MS analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Fisher Scientific) with buffer A (2:98 MeCN:H2O in 0.1% formic acid ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 200ng of tryptic peptides were analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS) using Ultimate 3000 UHPLC (ThermoFisher) connected to an Orbitrap Fusion Lumos Tribrid (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... The dried peptides were resuspended in 200 mM EPPS pH 8.5 and quantified by bicinchoninic acid assay using the quantitative colorimetric peptide assay from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: Digested peptides were analyzed by Orbitrap Fusion Lumos mass spectrometer coupled with EASY-nLC™ 1200 system (Thermo Fisher Scientific) for PRM ...