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Citations for Thermo Fisher :
4051 - 4100 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... After rinsing in PBS and incubating in blocking buffer (10% normal goat serum, Cat #PCN5000 and 0.2% Triton™ X-100 Cat #9002-93-1, ThermoFisher, in PBS) for 30 mins ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 3 x 10 min in PBS and incubated in goat anti-rabbit Alexa Fluor 647 (1:500 in blocking buffer; catalog no. A32733, Invitrogen, Carlsbad, CA) for 2 hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... washed 3 times with blocking buffer and incubated for 2 h with Alexa 647 anti-rabbit (1/250, A21247, Thermo Fisher Scientific). After washing the cells 3 times with PBS ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems) in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
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bioRxiv - Molecular Biology 2021Quote: ... coverslips were washed 3 times in PBS + 0.1% Tween then incubated at room temperature in the dark for 1 hour in blocking buffer containing Alexa Fluor 546 goat anti-rabbit IgG (Invitrogen, A-11035) secondary antibody (1:1500 ...
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bioRxiv - Genomics 2021Quote: ... and then blocked for 1 hour with 1 ml of blocking buffer (wash buffer containing 3% fatty acid free BSA (Thermo Fisher, 126609)) ...
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bioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% PFA in phosphate-buffered saline (PBS) for 30 min and blocked with blocking buffer consisting of 10% horse serum (Life Technologies, 26050088), 0.1% gelatin ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed twice with PBS and incubated in the blocking buffer containing donkey anti-goat IgG antibodies conjugated with Alexa Fluor 568 (ThermoFisher Scientific, USA) diluted 1:400 for 2 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... samples were washed 3×10 mins in IF blocking buffer prior to mounting on to coverslides with ProLong Diamond Antifade Mountant with DAPI (ThermoFisher Scientific #P36962). Slides were allowed to cure overnight before imaging with either a Zeiss AxioObserver widefield microscope (20x ...
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bioRxiv - Neuroscience 2022Quote: ... samples were washed 3 x 5m in PBS and then incubated 1h at RT with the following secondary antibodies all diluted 1:1000 in blocking solution: donkey anti-rabbit Alexa Fluor 647 (ThermoFisher A-31573); goat anti-mouse Alexa Fluor 568 (ThermoFisher A-11031) ...
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bioRxiv - Developmental Biology 2022Quote: ... Secondary labelling was done for 1 hour in blocking buffer containing Alexa Fluor 488 goat anti-rabbit IgG (InVitrogen, Waltham, MA (A11008)) or Alexa Fluor 488 goat anti-rat (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 μg/mL Hoechst 33342 (Fischer Scientific BDB561908) in the donkey blocking solution before washing and mounting on slides with Immu-Mount (Thermo Fisher Scientific) and 1.5 mm coverslips (EMS).
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bioRxiv - Immunology 2022Quote: ... The next day the plates were washed three times with TBS and blocked for 30 min with Casein blocking buffer (Thermo Fisher Scientific). After washing the plates with TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody solution contained 2 secondary antibodies in blocking solution: Goat anti-mouse Alexa-Flour (AF) 568 (Invitrogen A-11004, 1:400), Goat anti-chicken AF-488 (Invitrogen A11039 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were kept in the dark in blocking solution containing donkey anti-rabbit Alexa Fluor ® 647 (1:500 ab150075 Invitrogen®) and Goat anti-mouse Alexa Fluor ® 488 (1:500 A11001 ThermoFisher® ...
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bioRxiv - Bioengineering 2020Quote: ... membranes were incubated in secondary antibody for 1 hr at RT (goat anti-rabbit HRP conjugated, 1:2500 in blocking solution; ThermoFisher Scientific, UK).
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with the blocking buffer and re-suspended in PBS containing 5 mg/ml BSA (Thermo Fisher Scientific, USA), 0.5 U/ml RNsing plus (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated for 1 h at room temperature in blocking solution containing anti-rabbit secondary antibody conjugated to Alexa Fluor 488 (1:250 dilution) (Thermo Fisher Scientific). After an additional 3x washes ...
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bioRxiv - Bioengineering 2022Quote: ... wells were washed five times with PBS and blocked for 30 min with SuperBlock Blocking buffer (Life Technologies Europe BV, Bleiswijk, Netherlands) at 150 μL per well and at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were incubated overnight at 4°C in primary antibodies diluted in blocking buffer and after washing in PBST the blots were developed with the SuperSignal West Dura ECL kit (InVitrogen, Slangerup, DK). Primary antibodies were rabbit monoclonal anti-cleaved Notch1 (Val1744 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2.5X105 cells were used as controls and incubated in the blocking buffer while 6X105 cells were incubated with anti-BrdU monoclonal antibody (1:100; MOBU-1 clone, B35128, Thermo Fisher Scientific) for 1 hr at RT ...
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bioRxiv - Immunology 2020Quote: ... They were then exposed at room temperature first for 1 h to primary antibodies in blocking buffer and then for 1 h to Alexa Fluor–conjugated secondary antibodies (Thermo Fisher Scientific) and Hoechst 33342 in blocking buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with the blocking buffer and re-suspended in PBS containing 5 mg/ml BSA (Thermo Fisher Scientific, USA) and 0.5 U/ml RNsing plus (Promega ...
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bioRxiv - Neuroscience 2021Quote: Primary neurons on cover glasses or 20-µm cryostat-sectioned coronal brain slices were incubated for 1 hour at room temperature with blocking buffer [PBS containing 5% (v/v) goat serum (#16210-064, Gibco, New Zealand), 1% (w/v ...
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bioRxiv - Bioengineering 2019Quote: ... tissues were wash 3 times with PBS and incubate with protein blocking solution that contain 0.5% Triton X-100 (Fisher Scientific, Hampton, NH). Then tissues were incubated in 1:250 diluted primary antibody (CD31 (PECAM-1 ...
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bioRxiv - Genetics 2020Quote: ... they were incubated for 1 hour at room temperature with blocking solution containing the secondary antibodies (Alexa fluor 568 Goat anti-rabbit (1:800; Molecular Probes, #A11011) Alexa Fluor 488 goat anti-mouse (1:800 ...
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bioRxiv - Immunology 2021Quote: ... Plates were blocked with blocking buffer made up with 2% bovine serum albumin (BSA) Fraction V (Thermo Fisher Scientific, Waltham, MA, USA), 1% gelatin from bovine skin (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated for 2 h in blocking/permeabilization buffer including goat anti-rabbit AlexaFluor 568 or goat anti-mouse 488 (Invitrogen; 1:500). Sections were washed in 0.01 M PBS after primary and secondary antibody incubations ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were then washed 4 × 5 min with PBS followed by overnight incubation at 4°C with the following secondary antibody dilutions in blocking solution: Alexa 488-goat anti-rabbit (1:1000; Thermo Fisher Scientific) and Alexa 594-donkey anti-mouse (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... After 72h of incubation the sections were incubated with secondary antibodies diluted in blocking solution for two hours: Alexa 594 anti-rabbit (Invitrogen, 1:1000), Alexa 488 High Cross Absorbed (Invitrogen ...
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... with a cocktail of functionally inhibitory integrin blocking antibodies (20 μg/mL anti-α3, clone-ASC-6, Millipore Sigma; 20 μg/mL anti-α6, clone-GoH3, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... using primary antibodies diluted in blocking solution (VE-Cadherin, #14-1441-82, 5 μg/ml final and HES1, #PA5-28802, 5 μg/ml final, Thermo Fisher Scientific) for 48h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated for 2 hours at room temperature (22 ℃) in blocking solution containing the secondary antibodies Alexa Fluor® 555 donkey anti-rabbit (1:500, Thermo Fisher Scientific Cat# A-31570 ...
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bioRxiv - Neuroscience 2023Quote: ... After 1hr of incubation with blocking solution [0.1M tris buffered saline (TBS) with 1% bovine serum albumin (BSA, ThermoFisher Scientific, Waltham MA) and 5% dry milk] ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were processed for 48 hours at 4 degrees with the primary antibody in blocking solution (1:1250, rabbit anti-Esr1, Invitrogen PA1-309), rinsed 3×10 minutes in PBS ...
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bioRxiv - Immunology 2022Quote: ... Coverslips were washed with blocking solution 3 times at room temperature and incubated in blocking buffer containing Alexa Flour 488 anti-mouse IgG antibody (1:300; Invitrogen, MA, USA) for 1 h at room temperature in the dark ...
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bioRxiv - Microbiology 2022Quote: ... The lower molecular weight part of the membrane was incubated overnight at 4ºC in anti-6X-HisTag antibody (1:1000 dilution in blocking buffer, Invitrogen MA1-21315-HRP) to stain for SsrB protein constructs ...
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bioRxiv - Neuroscience 2022Quote: ... Blots to be probed with the #1 antibody were blocked in 5% milk while all others were blocked in Superblock Blocking Buffer (ThermoFisher, Cat#37515), each with 0.1% Tween 20 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were blocked for 1 hour at room temperature with Odyssey Blocking Buffer (LiCor, USA) before being probed overnight with primary antibodies against C9ORF72 (1:1000, 22637-1-AP, Thermo Scientific, USA). Infrared labeled secondary antibodies were applied ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation in an Alexa Fluor (AF) 488-conjugated goat-anti-rabbit secondary antibodies in the blocking solution (dilution 1:200, ThermoFisher, A-11008) for 2 hours ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The ovaries were then stained for 12 hours using the following reagents at 1:500 dilution in blocking solution: Hoechst 33342 (10 mg/ml, Thermo Fisher H3570), Wheat Germ Agglutinin-647 (WGA ...
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bioRxiv - Microbiology 2024Quote: ... coli type I fimbria or FliC (diluted 1:10 000 in protein-free tris-buffered saline (TBS) blocking solution (Fisher Scientific, France)) for 1 h at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... for HSV-1 in blocking buffer for 1 h at 4°C and stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer at RT for 1h ...
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bioRxiv - Neuroscience 2024Quote: ... and then cells were washed with ice-cold blocking solution and incubated with secondary antibody (goat anti-rabbit with Alexa Fluor 555, 1:1000, Thermo Fisher Scientific). Cells were then washed with ice-cold PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... sections were washed for 5 minutes in PBS and immediately incubated in blocking solution for 30 minutes (Pierce Super Blocker, Thermo Fisher Scientific). Sections were then incubated in a primary antibody cocktail for 60 minutes containing 1x PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Permeabilized embryos were incubated in blocking buffer (0.2% Triton X-100 in PBS + 2% BSA Fraction V 7.5% (Thermo Fisher Scientific, 15260-037) + 5% goat serum (Jackson Immunoresearch/Dianova ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA pol I and II visualization was accomplished by blocking (2) and incubating samples with rabbit anti-p-UBF1 (1:100 dilution; Invitrogen, PA5-105512) and mouse anti-RNA pol II (1:100 dilution ...
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bioRxiv - Microbiology 2024Quote: ... then the following secondary antibodies were diluted 1:500 in blocking buffer and incubated for 1h with the cells: anti-rabbit DyLight 448 (Thermo Scientific, 35552), anti-rabbit DyLight 550 (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer) for 1 hour at room temperature and detected using SuperSignal Western Dura PLUS Chemiluminescent Substrate (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2023Quote: ... and the following secondary antibodies diluted in blocking buffer: donkey anti-rat Alexa Fluor 488 (1:400, A-21208, Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 555 (1:400 ...