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Citations for Thermo Fisher :
3801 - 3850 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... peptides were recovered in HEPES buffer by collecting supernatant on magnet and combining it with a second elution wash of the magnetic beads with HEPES buffer. Peptides were labelled with TMT10plex (Werner et al. 2014) Isobaric Label Reagent (ThermoFisher) according the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The digested peptides were analyzed by reverse phase LC-MS/MS on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) coupled to a Dionex UltiMate 3000 -nLC (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were then introduced by nanoelectrospray into a LTQ Orbitrap Velos Pro coupled to an Easy-nLC HPLC (Thermo Fisher) over a linear gradient of acetonitrile at 250 nl/min over 140 min ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides from each sample were labelled using the ten-plex TMT reagent kit (90110, Thermo Fisher Scientific, Waltham, MA, US). Two ten-plex experiments were performed ...
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bioRxiv - Biophysics 2020Quote: ... or RGD-containing peptide (Peptite-2000; 100 μg mL−1 in PBS) in 1× PBS with Ca2+ (DPBS, Catalog No. 14040133, Gibco). Unattached beads were removed by washing with 1× PBS.
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bioRxiv - Biophysics 2021Quote: ... were vacuum-dried and resuspended with the peptide fractionation buffer [(70% (v/v) LC-MS grade water (Thermo Fisher Scientific), 30% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... The first set of LC-MS/MS was used to identify highly abundant unoxidized peptides using the Sequest search engine (Proteome Discoverer 1.4, Thermo Scientific) against the Uniprot Rattus Norvegicus database (downloaded on March ...
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bioRxiv - Biochemistry 2022Quote: ... and the resulting peptides were trapped and desalted on a 2 mmL×L10 mm C8 trap column (Hypersil Gold, ThermoFisher). The bound peptides were then gradient-eluted (4-40% CH3CN v/v and 0.3% v/v formic acid ...
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bioRxiv - Cancer Biology 2022Quote: Peptides were injected onto a trapping column for preconcentration (Acclaim Pepmap100 20cm x 75 μm, 3μm C18, Thermo Fisher Scientific), followed by nanoflow LC (Thermo Dionex ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Identifications and retention times were used to guide the manual quantification of each modified peptide using QualBrowser version 2.0.7 (Thermo Fisher Scientific). Site assignment was evaluated from MS2 spectra using QualBrowser and MaxQuant Viewer ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted peptides were then subjected to electrospray ionization directly coupled to a high-resolution Orbitrap mass spectrometer (QExactive, ThermoFisher).
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bioRxiv - Immunology 2022Quote: ... were pulsed for 1 hr with 10μg/ml influenza virus NP(366-374) peptide and were subsequently labeled for 10 min at 37°C with 5 μM CFSE (Invitrogen) (CFSEhigh ...
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bioRxiv - Neuroscience 2022Quote: ... Constant flow of 10 μl/min was maintained for peptide loading onto a pre-column (PepMap 100; Thermo Fisher Scientific). Peptides were then separated on a 10 mm PicoTip™ fused silica emitter 75 μm x 16 cm (New Objective Inc. ...
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bioRxiv - Molecular Biology 2022Quote: Digested peptides were acidified with 0.8% TFA (final) and analyzed by LC–MS/MS on an EASY-nLC 1000 (Thermo Scientific) with a two-column set-up ...
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bioRxiv - Pathology 2022Quote: ... Then peptide mixtures of each sample were labeled using the TMTsixplex reagent according to the manufacturer’s instructions (Thermo Fisher Scientific). Samples were labeled with the TMT tags ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Beta RBD for crystallization (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal 8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells in the presence of 10 µM kifunensine at 37°C and 8% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-chlamydial MreB antibody (custom anti-peptide antibody directed against the C-terminus of C. trachomatis serovar L2 MreB; ThermoFisher), or rabbit anti-six histidine tag (Genscript ...
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bioRxiv - Genetics 2022Quote: ... The peptides were dissolved in 2% formic acid and analysed on a Q-Exactive HF mass spectroscopy (Thermo Fisher Scientific). The peptides were separated using an EASY-Spray PepMap RSLC C18 column at flow rate of 300 nL/minute on a 60 min linear gradient of the mobile phase (5% acetonitrile (ACN ...
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bioRxiv - Biophysics 2022Quote: The GyrA14-E487C and the CcdA50-72 peptide (Table S1) were labeled with Fluorescein-5-Maleimide and 5-TAMRA (Tetramethylrhodamine-5-Maleimide) from ThermoFisher Scientific as per the manufacturer’s protocol as described previously (Aghera et al. ...
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bioRxiv - Microbiology 2022Quote: ... the LC-MS/MS data were used as the input for peptide identification using the database search engine SEQUEST HT implemented in Proteome Discoverer 2.2 (Thermo Scientific). Note ...
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bioRxiv - Cell Biology 2022Quote: ... Chromatographic separation of the peptides was performed by liquid chromatography on a nano-UHPLC system (Ultimate UHPLC focused, Thermo Scientific) using a nano column Acclaim PepMapC100 C18 ...
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bioRxiv - Cancer Biology 2022Quote: Detection of eluting peptides is performed using both UV at 214 nm (3nl UV cell, Thermo Fisher Scientific, Germering, Germany) and mass spectrometry (Q-Exactive Plus ...
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bioRxiv - Cell Biology 2022Quote: ... Fractionated peptides were ionized and analyzed by tandem mass spectrometry (MS/MS) Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific). Label-free quantitation was performed using the MaxQuant software package.79 The EMBL Human reference proteome (UP000005640 9606 ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were loaded first onto an Acclaim PepMap 100 Trapping column (Thermo Scientific, 20 mm 75 µm inner diameter (ID), 3 µm of C18 resin with 100 Å pore size ...
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bioRxiv - Neuroscience 2020Quote: ... the peptides from each sample were reacted for 1 h with the a ppropriate TMT-NHS isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were initially trapped in C18 PepMap100 pre-column (300 µm inner diameter x 5 mm, 100A, Thermo Fisher Scientific) in Solvent A (Formic acid 0.1% (v/v) ...
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bioRxiv - Molecular Biology 2020Quote: ... coli digests and Promega yeast protein digest were each fractionated by offline reversed-phase LC (Waters Acquity UPLC Peptide BEH300 C18 1.7 µm, 1 mm i.d. x 150 mm column employing an Ultimate 3000 HPLC, Thermo Fisher Scientific) operating with column compartment temperature of 60 °C and flow rate of 100 µL/min ...
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bioRxiv - Immunology 2020Quote: ... containing the gp67 secretion signal peptide and a C-terminal 6×His tag was inserted into pFastBac-Dual vectors (Invitrogen) and transformed into DH10Bac component cells ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody was affinity purified on phospho-peptide covalently bound to a SulfoLink column according to the manufacturer’s instructions (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: Peptide fractions were analysed in a Q Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA) coupled on-line with a nanoflow ultrahigh-performance liquid chromatography (nano-UPLC ...
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bioRxiv - Neuroscience 2020Quote: ... Per measurement 0.5 μg of peptides were automatically loaded to the online coupled RSLC (Ultimate 3000, Thermo Fisher Scientific Inc.) HPLC system ...
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bioRxiv - Bioengineering 2021Quote: Around 1 µg of peptides were run on the pre-column (Acclaim PepMap™ 100, 75μm × 2cm, nanoViper, Thermo Scientific) and the analytical column (Acclaim PepMap™ RSCL ...
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bioRxiv - Biochemistry 2021Quote: ... The application of a 2.5 kV distal voltage electrosprayed the eluting peptides directly into LTQ linear ion trap mass spectrometers (Thermo Scientific) equipped with a custom-made nano-LC electrospray ionization source ...
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bioRxiv - Biochemistry 2020Quote: Both NPC1 and synthetic peptide samples were analyzed by LC-MS using a Fusion Lumos mass spectrometer (Thermo Fisher Scientific) equipped with a Dionex Ultimate 3000 LC-system (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... was constructed by cloning the 2A peptide-linked Cas9 ORF from the GFP_2A_Cas9 expression vector46 into a pcDNA™3.1(+) vector (Thermo Fisher Scientific) between the HindIII and BamHI sites ...
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bioRxiv - Neuroscience 2022Quote: ... All peptide samples were analyzed on an Orbitrap Fusion Lumos Tribrid Mass Spectrometer with an Ultimate3000 RSLCnano system (Thermo Scientific). They were loaded C18 particles at 8 μL/minute flow rate ...
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bioRxiv - Developmental Biology 2022Quote: ... Analyses of peptide digests were carried out on an Orbitrap Q Exactive HF-X instrument (Thermo Fisher Scientific, Bremen, Germany). The instrument was configured for data-dependent methods using the full MS/DD?MS/MS setup in a positive mode ...
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bioRxiv - Molecular Biology 2022Quote: ... 5μl of each peptide extracts were loaded in two or three injection replicates onto 300μm ID x 5mm PepMap C18 precolumn (ThermoFisher, Dionex) at 20 μl/min in 2% acetonitrile ...
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bioRxiv - Immunology 2022Quote: For LC-MS analysis of wild type ILC2 1.5 µg of peptide for each sample was analysed on a Q-Exactive-HF-X (Thermo Scientific) mass spectrometer coupled with a Dionex Ultimate 3000 RS (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were analyzed using a 140 min water-acetonitrile gradient delivered by a Dionex Ultimate 3000 UHPLC (Thermo Fisher Scientific) operated initially at 400 nL/min flow rate with 1% buffer B (acetonitrile solution with 3% DMSO and 0.1% formic acid ...
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bioRxiv - Molecular Biology 2019Quote: ... TAILS and secretome peptide mixtures were loaded onto an PepMap100 C18 precolumn (75 μm x 2 cm, 3μm, 100Å, nanoViper, Thermo Scientific) and separated on a PepMap RSLC C18 analytical column (75 μm x 50 cm ...
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bioRxiv - Systems Biology 2019Quote: ... The cross-linked peptides were then enriched by HILIC on the Dionex UltiMate 3000 Series HPLC instrument (Thermo Fisher Scientific) equipped with a TSKgel Amide-80 column (3 µm ...
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bioRxiv - Systems Biology 2019Quote: ... Labelled peptides from each set of replicates were then combined and cleaned up using SCX cartridge system (Applied Biosystems, USA) prior to 2D-LC-MS analysis.
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bioRxiv - Cancer Biology 2019Quote: The peptides were analysed on a Thermo Scientific™Orbitrap Fusion™ Tribrid™ Mass Spectrometer (Thermo Scientific, Bremen, Germany) interfaced with Easy-nLC 1000II nanoflow liquid chromatography system (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18, cartridge, 300 µm i.d.×5 mm, 5 µm) (ThermoFisher), and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID ...
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bioRxiv - Microbiology 2019Quote: ... Peptides mixtures were analyzed by a Q-Exactive Plus coupled to a Nano-LC Proxeon 1000 (both from Thermo Scientific). Peptides were separated by chromatography with the following parameters ...
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bioRxiv - Immunology 2020Quote: ... Reconstituted peptides were tested for endotoxin contamination using a Limulus Amebocyte Lysate Chromogenic Endotoxin Quantitation Kit (Thermo Scientific, UK #88282).
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bioRxiv - Cell Biology 2019Quote: Tryptic peptides were analyzed using an EASY-nLC 1000 coupled to a Q ExactiveTM Hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific). The peptides were resolved and separated on a 50 cm analytical EASY-Spray column equipped with pre-column over a 120 min gradient ranging from 8 to 38% of 0.1% formic acid in 95% acetonitrile/water ...
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bioRxiv - Microbiology 2019Quote: ... rabbit anti-MreBCT antibody (custom anti-peptide antibody directed against the C-terminus of C. trachomatis serovar L2 MreB; ThermoFisher), rabbit anti-Hsp60 (kind gift of Dr ...
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bioRxiv - Microbiology 2020Quote: The tryptic eluted peptides were dried by speed-vacuum centrifugation and then desalted onto StageTip C18 Pipette tips (Thermo Scientific) until the mass spectrometric analysis ...