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Citations for Thermo Fisher :
3951 - 4000 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Eluted peptides were separated using an Acclaim PepMap100 analytical column (2 μm, 50 cm × 75 μm ID; Thermo Fisher Scientific). Using a constant flow rate of 300 nL/min ...
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bioRxiv - Immunology 2023Quote: ... One microgram of peptide sample was loaded on a C18 column (15 cm, 3.0 μm Acclaim PepMap, Thermo Fisher Scientific), separated with a 0–40% gradient of buffer B (80% acetonitrile ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Each sample containing a 100 μg peptide mixture was labeled using tandem mass tag reagent according to the manufacturer’s instructions (Thermo Scientific). The negative control group samples were labeled C1 ...
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bioRxiv - Molecular Biology 2022Quote: ... spectra were subjected to peptide and protein identification using the MS Amanda search engine triggered by proteome discover (version 1.4., Thermo Scientific). Here ...
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bioRxiv - Biochemistry 2023Quote: LC-MS/MS analysis of TMT-labeled peptides was conducted on an Orbitrap Fusion Lumos tribrid mass spectrometer (Thermo Scientific) coupled with an Easy-nLC 1200 UPLC system (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: The raw LC-MS/MS data was analyzed against the Uniprot GaHV2 database (taxon 10390; 1300 sequences) using the Byonic peptide search algorithm (Protein Metrics) integrated into Proteome Discoverer 2.4 (Thermo Scientific). Optimal main search settings were initially determined with Byonic Preview (Protein Metrics ...
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bioRxiv - Molecular Biology 2023Quote: ... Both peptide mixtures were separately analysed on LC-MS/MS system (RSLCnano connected to Orbitrap Exploris 480; Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2023Quote: ... Conjugates were purified from excess of free antigenic peptides by several dialysis steps at 4 °C against PBS using Slide-A-Lyzer 20,000 MWCO dialysis cassettes (Thermo Scientific). STxB(70C)-SL8 and STxB(70C)-G15F conjugate formation and the absence of remaining free SL8 or G15F after purification were validated by UPLC-MS.
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bioRxiv - Neuroscience 2023Quote: ... An equal amount of each peptide sample was incubated with individual TMT plex reagents according to the manufacturer’s instructions (ThermoFisher Scientific). After incubating for 60 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: Peptides for the qualitative analysis were separated by reversed phase LC on an RSLCnano system (Ultimate 3000, Thermo Fisher Scientific) coupled online to an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were analysed by nanoflow-LC-MS/MS using a Orbitrap Q-Exactive-HF™ Mass Spectrometer (Thermo Scientific TM) coupled to a Dionex™ Ultimate™ 3000 ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were separated on a capillary column (0.075 mm × 500 mm, Acclaim Pepmap 100, reverse phase C18, NanoViper, ThermoFisher Scientific) according to a 2-40% buffer B gradient (0.1 % formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting peptides were analyzed by a top-15 data dependent LC-MS/MS strategy using a QExactive plus mass spectrometer (ThermoFisher). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Sep-Pak Vac tC18 solid phase extraction cartridges (Waters) and the concentration determined using the Pierce quantitative colorimetric peptide assay (ThermoFisher). Peptides were dried by vacuum centrifugation.
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bioRxiv - Biochemistry 2023Quote: ... and applied to TiO2 spin tips (3 mg peptides per tip) for phosphopeptide enrichment according to the manufacturer’s protocol (High-SelectTM TiO2 Phosphopeptide Enrichment Kit, A32993, Thermo Scientific). Eluted phosphopeptides were transferred to Lo-bind Eppendorf tubes ...
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bioRxiv - Biochemistry 2023Quote: ... endoprotease Lys-C and Trypsin digested peptides were analyzed on an LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, Bremen). The raw data were analyzed with MaxQuant (version 2.0.3.0) ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 25 µg of peptides for each sample was labeled with 0.2 mg of TMT-10plex reagents (Thermo Fisher Scientific) for 1 h at 25 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were injected on a PepMap 100 C18 trap column (100 µm × 2 cm, 5 µM particles, Thermo Fisher Scientific) and separated with an EASY-Spray analytical column (PepMap RSLC C18 ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were eluted and separated on an C18 Acclaim PepMap RSLC analytical column (75 µm x 250mm, Thermo Fisher Scientific) with a flow rate of 300 nl/min in a 120 min gradient of 3% buffer A (0.1% formic acid ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged proteins were then eluted with room temperature Elution Buffer with 2.5 mg/mL 3XFLAG Peptide (Thermo Fisher # A36806) for 15 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: The enriched and unenriched samples were fractionated with the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to manufacturer instructions for TMT-labeled peptides ...
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bioRxiv - Genetics 2023Quote: ... Bin3-3xHA complexes were purified by two sequential competitive elutions with 75 µl of Pierce HA Peptide (Thermo Scientific, 26184) diluted to 2 mg/ml in Nuclease-Free Water (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed and then treated with peptide in NP-40 and Halt™ Protease and Phosphatase Inhibitor Cocktail (ThermoFisher) for 10 minutes at room temperature while rocking ...
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bioRxiv - Biochemistry 2023Quote: ... The digestion was quenched in 0.5% formic acid and spiked with Pierce Retention Time Calibrant peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/µL ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were analyzed using a 140 min water-acetonitrile gradient delivered by a Dionex Ultimate 3000 UHPLC (Thermo Fisher Scientific) operated initially at 400 nL/min flow rate with 1% buffer B (acetonitrile solution with 3% DMSO and 0.1% formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 200 ng peptide material was measured on a Q Exactive HF-X Orbitrap mass spectrometer (Thermo Fisher), coupled to the LC-system with a nano-spray ion-source using coated emitter tips (PepSep ...
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bioRxiv - Genetics 2023Quote: ... Each peptide extract was loaded on a 300 µm ID x 5-mm PepMap C18 pre-column (Thermo Scientific, USA) at a flow rate of 10 µL/min ...
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bioRxiv - Microbiology 2023Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Fisher Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were separated on a PepmapTM C18 reversed phase column (3 μm, 100 Å, 75 μm × 50 cm) (Thermo Scientific). Samples were fractionated with mobile phase A consisting of 0.1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were eluted and ionized by electrospray followed by mass spectrometric analysis on an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher). MS data were acquired using the FTMS analyzer and MS/MS data using the ion trap analyzer ...
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bioRxiv - Plant Biology 2023Quote: Peptide/Protein identification and MS1-based label-free quantification were carried out using Proteome Discoverer 2.5 (PD2.5) (Thermo Fisher Scientific) or Skyline version 22.2 (MacCoss lab software) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed with warmed PBS before the addition of peptide in DMEM (without phenol red) with 10% FBS (ThermoFisher) for 2-4 hours at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and digested tryptic peptides were analyzed by LC-MS/MS on Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Fisher Scientific) using the same configuration and settings as previously reported85 ...
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bioRxiv - Biochemistry 2023Quote: ... Each sample of peptides was loaded onto a C18 trap column (75 μm ID × 2 cm, 3 μm, Thermo Scientific) and then separated on a C18 analytical column (75 μm ID×50 cm ...
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bioRxiv - Biochemistry 2023Quote: ... Proteolytic peptides were finally concentrated in a trapping column (C18 Pepmap, 300 μm i.d. × 5 mm length, Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Samples were then separated into 8 fractions using the High pH reversed-phase peptide Fractionation Kit (ThermoFisher Scientific, cat# 84868). The fractions were dissolved with 0.1% formic acid and peptide concentration was determined with Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Non-deuterated samples peptide identification was performed via data dependent tandem MS/MS experiments and analyzed by Proteome Discoverer 2.5 (Thermo Fisher). Mass analysis of the peptide centroids was carried by HDExaminer v3.3 (Sierra Analytics ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped in a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMap 100, Thermo Fisher Scientific) with buffer A (2/98 MeCN:H2O in 0.1% formic acid ...
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bioRxiv - Genetics 2023Quote: ... Peptides were separated using reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific, Waltham, MA) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: TMT-labeled peptides were analyzed using an EASY-nLC 1200 UPLC coupled with an Orbitrap Fusion Lumos mass spectrometer (ThermoFisher). LC buffer A (0.1% formic acid ...
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bioRxiv - Biochemistry 2023Quote: ... MS data of the peptides were acquired on a Q Exactive mass spectrometer coupled to EASY-nLC (Thermo Fisher Scientific) using a data-dependent top 10 methods that dynamically choose the most abundant precursor ions from the survey scan (300–1800 m/z ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were desalted with an Oasis PRiME HKB μElution Plate and analysed on a QExactive HF HPLC-MS (Thermo Scientific). Separation by HPLC prior to MS occurred on an Easy-nLC1200 system (Thermo Scientific ...
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bioRxiv - Physiology 2023Quote: ... the labeled sample was separated into five fractions using the high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific). Bound peptides were eluted with 1 ...
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bioRxiv - Biochemistry 2023Quote: We acidified the digested samples with concentrated trifluoroacetic acid (TFA) and cleaned up the peptides with C18 SOLA Solid-Phase Extraction (SPE) columns (ThermoFisher). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were further separated onto an Easy-Spray RSLC C18 column (75 µm i.d., 50 cm length, ThermoFisher Scientific) using a 30 min linear gradient (15% to 35% solvent B (0.1% formic acid in acetonitrile) ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were loaded onto a trapping cartridge (Acclaim PepMap C18 100Å, 5 mm x 300 µm i.d., 160454, Thermo Scientific) in a mobile phase of 2% ACN ...
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bioRxiv - Microbiology 2023Quote: ... and peptide concentrations for each sample were estimated based on absorbance measurements at 280 nm (NanoDrop™, Thermo Fisher Scientific). For each sample ...
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bioRxiv - Immunology 2022Quote: Peptide separation in buffer A (0.1% formic acid) was achieved using an EASY-nLC 1200 HPLC system (Thermo Fisher Scientific) and a column (75 µm inner diameter ...
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bioRxiv - Biochemistry 2023Quote: ... 20 μg of peptides from each sample were reacted for 1 h with the appropriate TMT-NHS isobaric reagent (ThermoFisher) in 40% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... An internal standard was prepared from the isotope-labeled standards in 3% acetonitrile with 0.1% formic acid with 5 fmol/µL of Peptide Retention Time Calibration (PRTC) mixture (ThermoFisher Scientific) and 10 µg/mL E ...