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Citations for Thermo Fisher :
3901 - 3950 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... peptides were labeled in DMSO using ~1.3-fold molar excess of Oregon Green 488 maleimide (Life Technologies/Molecular Probes #O6034) dissolved in a 1:1 solution of acetonitrile:DMSO ...
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bioRxiv - Cancer Biology 2022Quote: ... peptides in 1% (v/v) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (ThermoFisher Scientific, Inc). After washing with 0.5% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Cross-linked peptides were analyzed by an Easy nanoLC system coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) using data-dependent acquisition of ions with a charge state between 3 and 8 and HCD fragmentation using stepped normalized collision energy (NCE ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were first separated by an Ultimate 3000 RSLC nano and a Q-Exactive Plus mass spectrometer (both Thermo Scientific). Settings were set as described previously (https://pubmed.ncbi.nlm.nih.gov/30638812/) ...
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bioRxiv - Biochemistry 2022Quote: Peptides were resuspended in 0.5% formic acid and analysed using nanoflow LC-MS/MS using an Orbitrap Elite (Thermo Fisher) hybrid mass spectrometer equipped with a nanospray source ...
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bioRxiv - Plant Biology 2022Quote: ... The peptides were loaded onto a reversed phase trap column (Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18; Thermo Scientific) connected to the C18-reversed phase analytical column (EASY-Column ...
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bioRxiv - Microbiology 2022Quote: ... The peptide eluent from the C18 column was electrosprayed in Thermo Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific, USA) in positive ion mode using 2.2 kV voltage ...
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bioRxiv - Immunology 2022Quote: ... Peptides were separated by reverse phase chromatography using a C18 pre-column (Acclaim PepMap 100, 75μm×2cm, Thermo Fisher Scientific) followed by C18 reversed-phase LC column (PepMap RSLC C18 ...
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bioRxiv - Immunology 2022Quote: ... 106 splenocytes were ex vivo stimulated with SARS-CoV-2 peptide pool (JPT PM-WCPV-S-1) at concentration of 2 μg/ml of each peptide in RPMI (Biowest) supplemented with 10% heat inactivated FBS (Gibco). PMA/Ionomycin (BD Pharmingen™ ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were first trapped onto a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with Buffer A at a flow rate of 2.5 µL/min over 4 min ...
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bioRxiv - Molecular Biology 2022Quote: ... fractions containing tryptic peptides were resolved and ionized using nanoflow UHPLC (Easy-nLC 1200 system, Thermo Fisher Scientific Inc., USA) coupled to an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific Inc. ...
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bioRxiv - Systems Biology 2022Quote: ... The peptides were fractionated using high-pH HPLC methods and were then analyzed in Orbitrap Fusion™ Mass Spectrometer (ThermoFisher).
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bioRxiv - Systems Biology 2022Quote: ... Peptide analyses (corresponding to 1 µg protein) were performed on a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific) connected to an EASY-nLC 1200 ultra-HPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... VTWFHAIHVSGTNGT31) immunodominant spike peptides (1 μg/mL) overnight at 37°C in the presence of Brefeldin A (1:500, Invitrogen). The following day ...
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bioRxiv - Neuroscience 2023Quote: ... peptides were separated on a 250 mm x 75 μm Acclaim PepMap® C18 reverse phase analytical column (Thermo Scientific) over a 150 minutes organic gradient ...
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bioRxiv - Neuroscience 2023Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap® C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Microbiology 2023Quote: Peptides for proteome analyses were separated by reverse phase liquid chromatography (LC) on an RSLCnano system (Ultimate 3000, Thermo Scientific) coupled online to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) ...
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bioRxiv - Physiology 2023Quote: ... Carcasses were then incubated with 1:200 Alexa fluor 488-conjugated phalloidin (a bicyclic peptide that binds F-actin) (A12379, Invitrogen) for 40 minutes at 4°C and washed as described above ...
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bioRxiv - Biochemistry 2022Quote: ... the peptide concentration for the two lowest temperatures was spectrophotometrically determined at 280 nm using a NanoDrop instrument (Thermo Scientific) and the average used as the concentration for all samples ...
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bioRxiv - Genetics 2022Quote: ... Peptides were collected by centrifugation and labeled using Tandem Mass Tag (TMTpro 16plex) reagents (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Desalted peptides were injected in an EASY-Spray™ PepMap™ RSLC C18 50cm X 75cm ID column (Thermo Scientific) connected to an Orbitrap Fusion™ Tribrid™ (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides were first trapped onto a C18 column (75 µm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (0.1% formic acid ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides (2 μg/ sample) were injected onto a nanoscale C18 reverse-phase chromatography system (UltiMate 3000 RSLC nano, Thermo Scientific) and electrosprayed into an Orbitrap Exploris Mass Spectrometer (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Non-deuterated samples peptide identification was performed via tandem MS/MS experiments and analyzed by Proteome Discoverer 2.5 (Thermo Fisher). Mass analysis of the peptide centroids was carried by HDExaminer v3.1.2 (Sierra Analytics ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMap™ 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were introduced using an EASY-Spray source at 2000 V to a Fusion Tribrid Orbitrap mass spectrometer (Thermo Scientific). Full MS spectra were recorded from 300 to 1500 m/z in the Orbitrap at 120,000 resolution with an automatic was performed using TopSpeed mode at a cycle time of 3 s ...
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bioRxiv - Cell Biology 2022Quote: ... The peptides were collected by centrifugation and isobaric labeling was performed using Tandem Mass Tag (TMT-10plex) reagents (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Peptides were analyzed using a 140 min water-acetonitrile gradient delivered by a Dionex Ultimate 3000 UHPLC (Thermo Fisher Scientific) operated initially at 400 nL/min flow rate with 1% buffer B (acetonitrile solution with 3% DMSO and 0.1% formic acid ...
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bioRxiv - Neuroscience 2022Quote: ... peptides from each individual case and the GIS pooled standards were labeled using the TMTpro 16-plex kit (ThermoFisher 44520). Each batch in Set1 comprised 2 TMT channels with labeled GIS standards with all other channels reserved for individual brain samples ...
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bioRxiv - Neuroscience 2022Quote: ... peptides from each individual case and the GIS pooled standards were labeled using the TMT 10-plex kit (ThermoFisher 90406). For Set2 (14 batches) ...
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bioRxiv - Microbiology 2022Quote: ... reduced and alkylated followed by overnight trypsin digestion and peptide extraction following manufacturer’s instructions (In-Gel Tryptic Digestion kit, ThermoFisher Scientific). Extracted peptides were vacuum dried and redissolved in 2% acetonitrile in 0.1% formic acid in water.
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bioRxiv - Systems Biology 2024Quote: ... Peptides was were loaded via an Acclaim PepMap 100 trap column (C18, 5 μm, 100Å; Thermo Scientific, San Jose, CA) onto a Waters BEH C18 column (100 µm × 100 mm ...
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bioRxiv - Microbiology 2024Quote: Peptide mixtures were analyzed using two-dimensional (2D) liquid chromatography (LC) on an Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) coupled with a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: Sorted lung DC1 were loaded with 10 nM MHC class I-restricted OVA peptide (OVA257-264, SIINFEKL) in complete IMDM media (Gibco) for 1.5 hours at 37°C in a Eppendorf tube ...
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bioRxiv - Developmental Biology 2024Quote: Peptides were detected by tandem mass spectrometry using a Q Exactive Plus hybrid quadrupole-orbitrap mass spectrometer (Thermo Fisher Scientific). The Nanospray Flex ion source (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... coupled to an UltiMate 3000 HPLC system for on-line liquid chromatographic separation .Tryptic peptides for each sample were loaded onto a C18 trap column (ThermoFisher Scientific Acclaim PepMap 100 ...
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bioRxiv - Molecular Biology 2024Quote: ... digested peptides were acidified with 0.8% TFA (final) and analyzed by LC– MS/MS on an EASY-nLC 1000 (Thermo Scientific) with a two-column setup ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were applied on an Acclaim PepMap 100 C18 trap column (75 µm ID × 2 cm, 3 µm; Thermo Scientific) in 0.1% formic acid and 2% acetonitrile in H2O at a constant pressure of 80 MPa and separated by a linear gradient of 2%–6% buffer B in buffer A for 3 min ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides was dissolved in buffer A (0.1% Formic acid) loading into a a reverse phase trap column (Thermo Scientific Acclaim), the column was connected to a C18-reversed phase analytical column (Thermo Scientific Easy Column) ...
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bioRxiv - Microbiology 2024Quote: Digested peptides were analyzed with 90-minute gradients in C18 nanoflow reversed-phase HPLC (Dionex Ultimate 3000,3500 RSLC nano, Thermo Scientific) combined with orbitrap mass spectrometer (Q Exactive Orbitrap HF ...
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bioRxiv - Cancer Biology 2024Quote: ... the resin underwent trypsin digestion and peptides were run on an Orbitrap Fusion™ Lumos™ mass spectrometer (ThermoFisher Scientific) at the UNMC Proteomics Core and analyzed using Proteome Discoverer (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Coding regions of IPI-O1 and IPI-O1nlswithout a signal peptide and StCWC15a/b were amplified with primers containing attB tails and BP-cloned into pDONR207 (Invitrogen). To generate BiFC constructs ...
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bioRxiv - Genetics 2024Quote: ... Sample preparation and labeling of the peptides with TMT isobaric mass tags was performed as manufacturer’s instructions (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2024Quote: ... 25,000 rested T cells were co-cultured with DC pulsed with 10 ug/ml cognate peptides or treated with PHA as a positive control (1:100, ThermoFisher). After 5 days ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were loaded with autosampler and trapped in an Acclaim PepMap 100 trap column (75µm ξ 2 cm, Thermo Scientific) at 4 µL/min with 100% solvent A (0.1% formic acid in water ...
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bioRxiv - Cell Biology 2023Quote: LC-MS/MS analyses of peptide mixtures coming from in-gel digestions were done using RSLCnano system (Thermo Fisher Scientific) on-line connected to Impact II Qq-Time-Of-Flight mass spectrometer (Bruker ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped on a µPrecolumn C18 PepMap100 (5 μm, 100 Å, 300 μm×5 mm, ThermoFisher Scientific, Reinach, Switzerland) and separated by backflush on a C18 column (5 μm ...
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bioRxiv - Biophysics 2024Quote: ... Eluting peptides were analyzed in data-dependent acquisition mode on an Orbitrap Eclipse mass spectrometer (Thermo Fisher Scientific, Dreieich, Germany) coupled to the nano-HPLC by a Nano Flex ESI source ...
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bioRxiv - Biophysics 2024Quote: ... Eluting peptides were analyzed in data-dependent acquisition mode on an Orbitrap Eclipse mass spectrometer (Thermo Fisher Scientific, Dreieich, Germany) that is connected to the nano-HPLC by a Nano Flex ESI source ...
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bioRxiv - Cell Biology 2024Quote: ... and phospho-peptides were subjected to LC-MS/MS analysis using an Orbitrap QE LC-MS/MS system (Thermo Scientific) at the proteomics core facility of UT Southwestern Medical Center ...