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Citations for Thermo Fisher :
4101 - 4150 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... this was followed by the incubation in the blocking solution containing secondary antibody goat anti-Rabbit DyLight 680 (ThermoFisher, cat. no. 35568) or/and donkey anti-Goat DyLight 680 (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... then immersed in diluted blocking buffer containing Alexa-Fluor-conjugated secondary antibodies (Goat anti-Rabbit and Goat anti-Mouse IgG1, Invitrogen / Thermo-Fisher), each at 1:800 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibodies were diluted in blocking buffer as follows: Alexa Fluor 488-Goat anti-Mouse IgG (H+L) secondary antibody (1:1000; Life Technologies; A11029) an Alexa Fluor 568-Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were incubated for 1 hour in blocking buffer containing goat αrabbit IgG (H+L) secondary antibody Alexa Fluor 568 conjugate (Thermo Fisher Scientific). Again ...
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bioRxiv - Bioengineering 2023Quote: Three vibratome slices from three goat eyes were incubated for 4 hours in 5% BSA blocking solution without permeabilization agent, overnight in GFAP primary antibody (rabbit origin, (Invitrogen, PA5-16291) at 1:200 in blocking solution ...
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bioRxiv - Bioengineering 2022Quote: ... Incubation with secondary antibodies diluted in blocking solution was performed for 2 h at RT: Anti-mouse-Alexa 647 (1:500, cat. A32787, ThermoFisher Scientific Inc.), Alexa Fluor 488-Phalloidin (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... followed and the slides were incubated with secondary antibody (1:400 in blocking buffer, donkey a-rabbit Alexa 488 antibody, Invitrogen A-21206; or goat a-mouse Alexa488, Invitrogen A-11001) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... slides were washed with TBST and incubated with secondary antibodies in blocking buffer [goat anti-rabbit (cat# A32731; Invitrogen, Waltham, MA, USA) or goat anti-mouse (cat# 8890 ...
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bioRxiv - Immunology 2023Quote: ... Detection was performed utilizing the following horseradish peroxidase-conjugated primary antibodies that were diluted 1:1000 in blocking buffer: goat anti-mouse IgG1 (A10551, Thermo Fisher Scientific), goat anti-mouse IgG2c (PA1-29288 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed four times in PBS and incubated for 45 min with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Donkey anti-Rabbit IgG, (#A10042, Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500, Alexa Fluor 568 Goat anti-Rabbit IgG, (#A11036, Thermo Fisher Scientific)) ...
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bioRxiv - Cell Biology 2022Quote: ... in blocking buffer and then for 2 hours at room temperature with Alexa Fluor–conjugated secondary antibodies (1: 1000, Thermo Fisher Scientific) in blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 3D artificial muscles were fixed with buffered 1% paraformaldehyde (PFA) (ThermoScientific, JI9943.K2) overnight at 4°C followed by 6 hours of blocking at 4°C [10% FBS (Gibco, 10270-106), 1% BSA (Sigma ...
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bioRxiv - Immunology 2023Quote: ... After three washes of blocking solution, cells were stained with goat anti-mouse (AlexaFluor594, A11005) and anti-rabbit (AlexaFlour488, A11034) antibodies (Invitrogen, 1:1,000) for 1h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... before being incubated at 4°C in Odyssey Blocking Buffer (PBS) containing mouse anti-V5 antibody (RRID: AB 2556564, R960-25; Thermo Fisher Scientific) at a 1:3000 dilution for detection of 3v5 tagged alleles of Ume6 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were subsequently washed with PBS and incubated with fluorophore-conjugated secondary antibodies in blocking solution [anti-rabbit IgG Alexa Fluor™ 594 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... and the following secondary antibodies diluted in blocking buffer: donkey anti-rat Alexa Fluor 488 (1:400, A-21208, Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 555 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked with blocking buffer and incubated with Alexa Fluor 594 or 568-conjugated streptavidin (ThermoFisher #S32356 or #S11226, 1:1000) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... DRG samples were incubated in the blocking solution with added primary antibodies: anti-GFP (Invitrogen, #11122, 1:400, 2 days, 37°C), and anti-V5 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... then washed with PBST and incubated with goat anti-mouse IgG (H+L) Alexa Fluor 594 (1:500 dilution by the blocking solution, A-11032, Thermo Fisher Scientific) at room temperature for at least 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... in the presence of Brilliant Stain Buffer Plus (10 μl/sample) (cat# 563795, Becton Dickinson, Mountain View, Ca, USA) and CellBlox Blocking Buffer (5 μl/sample) (cat# C001T02F01, Thermo Fisher Scientific). Cells were washed again in FACS buffer and intracellularly stained to detect transcription factor expression using the True-Nuclear Transcription Factor Buffer Set (cat# 424401 ...
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bioRxiv - Cell Biology 2024Quote: ... They were then exposed at room temperature first for 1 hour to primary Abs in blocking buffer and then for 30 minutes to Alexa Fluor–conjugated secondary Abs (Thermo Fisher Scientific) in blocking buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... the following secondary antibody was added overnight at 4°C in blocking buffer: Alexa FluorTM 647 donkey anti-rabbit IgG (1:1000, Invitrogen #A-32795). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 hour at room temperature and subsequently incubated in the blocking buffer containing rabbit anti-SYNGAP1 (1:5000; ThermoFisher #PA1-046), rabbit anti-SRGAP2A (1:5000 ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated with one or more of the following secondary antibodies in blocking solution for 30 minutes (Donkey anti-rabbit Alexa 488 [A21206; Fisher Scientific], Donkey anti-mouse Alexa Fluor 488 [A21202; Fisher Scientific] ...
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bioRxiv - Bioengineering 2024Quote: ... for 1 h at room temperature preceding primary antibody incubation using a 1:1 mixture of Odyssey Blocking Buffer and TBST (ThermoFisher, J77500.K8) diluted to 1x concentration at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: The slides were pre-incubated in blocking solution and stained against Griffonia simplicifolia isolectin GS-IB4 Alexa 568 (Invitrogen, I21412; 1:500), Neurofilament 200 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were washed in PBS for 5 minutes and subsequently incubated in blocking solution for 15 minutes (Pierce Super Blocker; Thermo Fisher Scientific). The slides were incubated in primary antibody solution (1x PBS ...
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bioRxiv - Bioengineering 2024Quote: ... Then secondary antibodies were then added in blocking buffer over night at 4 ºC (Goat anti Rabbit 647, Thermo Fisher Scientific A21245, 1:1000; Rhodamine Phalloidin, Fisher Scientific 50-646-256 ...
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bioRxiv - Genomics 2024Quote: ... and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP ...
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bioRxiv - Cell Biology 2024Quote: ... diluted 1:300 in the same blocking solution as well as the secondary antibody Alexa Fluor 647 (Cat. No. A21244, Life Technologies, USA). Plasma membranes were stained with wheat germ agglutinin (WGA ...
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bioRxiv - Developmental Biology 2024Quote: ... The hydrated slide is blocked in 3% skim milk PBST for 1 hour and incubated with primary antibody (1:100) diluted in Superblock Blocking Buffer in TBS (#37535, Thermo Fisher Scientific) at room temperature overnight ...
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bioRxiv - Plant Biology 2019Quote: ... Two μL (~1μg) peptides were analyzed by LC-MS with a Dionex RSLCnano HPLC coupled to a LTQ-Orbitrap Velos Pro (Thermo Scientific) mass spectrometer using a 2 h gradient ...
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bioRxiv - Biophysics 2021Quote: ... and the resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Biophysics 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Cancer Biology 2021Quote: Peptides were separated on an EASY-Spray™ Acclaim PepMap C18 column (50 cm × 75 μm inner diameter, Thermo Fisher Scientific) using a binary solvent system of 2% acetonitrile with 0.1% formic acid (Solvent A ...
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bioRxiv - Cancer Biology 2021Quote: ... The peptides were analyzed on a LC-MS/MS platform consisting of an Easy-nLC 1200 UHPLC system (Thermo Fisher Scientific) interfaced with an QExactive HF orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting peptides were analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Qexactive HFX mass spectrometer (Thermo Fisher Scientific) coupled to a nano-LC system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... the extracted peptides were completely dried to completion and thereafter desalted using the SPE Pierce C18 Spin Columns (Thermo Fisher Scientific). These columns were activated by 2 × 200 μL of 50% acetonitrile (CAN ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were passed through an immobilized pepsin column (2 mm□× 2 cm) at 120 μL min−1 and the digested peptides were captured on a C18 PepMap300 trap column (Thermo Fisher) and desalted ...
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bioRxiv - Developmental Biology 2020Quote: ... Approximately 1 μg of peptides from each digested band was analysed using LC-MS/MS on a Q Exactive mass spectrometer (ThermoFisher Scientific), as previously described (36).
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bioRxiv - Molecular Biology 2022Quote: ... Target peptides for each of these proteins were purchased in stable isotope labelled synthetic peptides (SIS) form in crude quality from Thermo Scientific. The C-terminal lysine or arginine in the SIS peptides were replaced by isotope labelled lysine (13C6 ...
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bioRxiv - Cancer Biology 2022Quote: ... Using a 6 to 30% gradient over 84 mins peptides were separated and analyzed using Thermo Fusion Lumos mass spectrometer (Thermo Scientific). Following parameters were used- MS1 ...
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bioRxiv - Cell Biology 2022Quote: Fractions of crosslinked peptides were analyzed by liquid chromatography coupled to tandem mass spectrometry using an LTQ Orbitrap Elite (Thermo Scientific) instrument 65 ...
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bioRxiv - Biochemistry 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Immunology 2019Quote: ... and resulting peptides were fractionated and sequenced by nanoflow LC-electrospray MS/MS on an Orbitrap Velos Pro hybrid mass spectrometer (Thermo Scientific), in the UT Austin mass spectrometry core facility as described previously (26) ...
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bioRxiv - Molecular Biology 2020Quote: ... High pH reversed fractionation was achieved by attaching a XBridge Peptide BEH C18 column (Cat. 186003570; Waters Corp.) to an Accela liquid chromatography system (Thermo Scientific) using the HPLC application ...
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bioRxiv - Molecular Biology 2020Quote: ... The column was enclosed into a column oven (Sonation) and the peptides were sprayed into a Q Exactive Plus mass spectrometer (Thermo Fisher). The mass spectrometer was operated in a data-dependent acquisition mode using a top10 method ...