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Citations for Thermo Fisher :
4251 - 4300 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: The resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Cancer Biology 2022Quote: ... Aliquots containing 0.5 μg peptides were separated on a reverse phase C18 column and were analyzed on QExactive HF Mass Spectrometer (Thermo-Fisher Scientific) as described previously 88 ...
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bioRxiv - Molecular Biology 2022Quote: ... The nTAP-MS peptides were applied on an Acclaim PepMap 100 C18 trap column (75 μm ID x 2 cm, 3 μm, Thermo Scientific) in 0.1% formic acid ...
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bioRxiv - Neuroscience 2022Quote: ... then the flow-throughs were fractionated into 8 fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 RSLCnano and Q-Exactive Plus, Thermo Scientific). Peptides were sampled on a 300 µm x 5 mm PepMap C18 precolumn and separated on a 75 µm x 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Plant Biology 2022Quote: ... Tryptic peptides were resuspended in H2O containing 0.1% formic acid and separated in an EASY-nLC1000 chromatographic system (Thermo Fisher Scientific) for 95 minutes using a C18 nano-column under a constant flow of 400 nL/min and increasing concentrations of acetonitrile (7 to 30% over 70 min and then 70% in 25 min) ...
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bioRxiv - Molecular Biology 2022Quote: ... The peptides were concentrated (online) by reverse phase chromatography using a 0.1 mm × 20 mm C18 RP precolumn (Thermo Fisher Scientific), and then separated using a 0.075mm x 250 mm C18 RP column (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... was cloned in frame with a N-terminal GFP tag and intervening short peptide linker (GSGEGRG) into the pcDNA5/FRT/TO bacterial expression vector (Invitrogen; V652020). A C-terminal histidine tag (Hisx6 ...
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bioRxiv - Biochemistry 2022Quote: ... The latter was performed in the National Laboratory of Research and Services in Peptides and Proteins using an LCQ DUO ESI ion trap (Thermo Finnigan) or QExactive Orbitrap (Thermo Scientific) spectrometers.
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bioRxiv - Biochemistry 2022Quote: ... Relative ion abundance of peptides labelled with 12C-IPA and/or 13C-IPA in extracted ion chromatograms generated using XCalibur Qual Browser software (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested with trypsin (at a 1:50 trypsin to protein ratio) and resulting peptides were desalted on a C18 Hypersep column (Thermo Scientific) and dried down using a speed-vac ...
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bioRxiv - Immunology 2022Quote: ... the captured viral biotinylated peptides were detected with 1 mg/ml pNPP (p-nitro-phenyl-phosphate, Thermo Fisher Scientific, cat: 34045) diluted in Diethanolamine substrate buffer 1X (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: The full-length CcdA and CcdA peptides of different lengths (Table S1) were biotinylated with EZ-linkTM NHS-Biotin (ThermoFisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were labeled in DMSO using ∼1.3-fold molar excess of Oregon Green 488 maleimide (Life Technologies/Molecular Probes #O6034, USA) dissolved in a 1:1 solution of acetonitrile:DMSO ...
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bioRxiv - Biochemistry 2022Quote: ... peptides were labeled in DMSO using ∼1.3-fold molar excess of Oregon Green 488 maleimide (Life Technologies/Molecular Probes #O6034, USA) dissolved in a 1:1 solution of acetonitrile:DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... An EASY nLC ultra-pressure liquid chromatography system was used to elute peptides onto a Fusion Tribrid mass spectrometer (Thermo Scientific). MS1 profiling was performed in a 375–1600 m/z range at a resolution of 70000 ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were loaded onto a μ-precolumn (Acclaim PepMap 100 C18, cartridge, 300 μm i.d.×5 mm, 5 μm) (Thermo Scientific), and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID ...
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bioRxiv - Cell Biology 2022Quote: ... In brief, peptides were loaded on a C18 PepMap100 pre-column (300 µm i.d. x 5 mm, 100Å (Thermo Fisher Scientific)) at a flow rate of 12 μl/min in 100% buffer A (0.1% formic acid in H20) ...
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bioRxiv - Neuroscience 2021Quote: ... Phospho-LIMK1/2 was detected with rabbit polyclonal anti-phospho-LIMK1 Thr508/ LIMK2 Thr505 directed against a synthetic phospho-peptide (Thermo Fisher Scientific # PA5-37629 ...
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bioRxiv - Biochemistry 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 minute organic gradient ...
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bioRxiv - Microbiology 2020Quote: ... The resulting tryptic and chymotryptic peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Molecular Biology 2020Quote: Sample aliquots comprising 25 μg of peptides were labelled with isobaric tandem mass tags (TMT 10-plex, Thermo Fisher Scientific, 90110) as described previously31 ...
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bioRxiv - Molecular Biology 2020Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides extracted from each band were analyzed by nanoLC-MS/MS using an Ultimate 3000 system (Dionex, Thermo Scientific, Waltham, MA) coupled to a TripleTOFTM 6600 mass spectrometer (ABSciex) ...
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bioRxiv - Cell Biology 2020Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min ...
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bioRxiv - Microbiology 2020Quote: MS3-TMT: Analysis of TMT labelled peptide mixtures was carried out on the Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Scientific) (control software Xcalibur™ ...
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bioRxiv - Physiology 2021Quote: LC-MS/MS analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2021Quote: LC-MS/SM analysis of digested peptides was performed on an Orbitrap Q Exactive HF mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A volume corresponding to 4 μg of the peptide mixture was separated by a Proxeon Easy-nLC System (Thermo Fisher Scientific) using an RP-C18-A2 column (3 μm) ...
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bioRxiv - Cancer Biology 2020Quote: ... and nuclear extracts were incubated for 1.5 hr at 4°C with 1 µg of H3K4ox (ART-K(OH)-QTARKSTGGKAP-biotin) or irrelevant (AHIVMVDAYKPTK-NH(CH2)2NH-biotin) peptides that had been previously recovered with streptavidin magnetic beads (Invitrogen; 65305) for 30 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 minutes organic gradient ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, 100Å, Thermo Scientific) before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
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bioRxiv - Pathology 2021Quote: 1.5 μg of peptide was injected on an UltiMate 3000 nano LC system online coupled to a Q-Exactive HF-X instrument (Thermo Scientific). Samples were transferred to a PepMap 100 C18 trap column (100 µm x 2 cm ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were ionized by nano-electrospray at 2 kV and analyzed on an Orbitrap Exploris 480TM (Thermo Fisher Scientific, Bremen, Germany) MS ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting peptides were resolved on a nano-capillary reverse phase column (Acclaim PepMap C18, 2 micron, 50 cm, Thermo Scientific) using a 1% acetic acid/acetonitrile gradient at 300 nl/min and directly introduced into Q Exactive HF mass spectrometer (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: Peptides were constructed on a CEM Liberty 12-Channel Automated Microwave Peptide Synthesizer using Fmoc-PAL-PEG-PSTM (Rink resin; loading capacity 0.21 mmol/g) (ThermoFisher, Cat.#: GEN913383) and Fmoc-protected amino acids (GL Biochem (Shanghai)) ...
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bioRxiv - Neuroscience 2022Quote: ... acetonitrile 0.1% (v/v) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Neuroscience 2022Quote: Chemicals and reagents specifically used for proteomic analysis include: Pierce™ high pH reversed-phase peptide fractionation kit (Cat. No.: 84868; ThermoFisher), High-Select™ Fe-NTA phosphopeptide enrichment kit (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were separated on an Ultimate 3000 RSLCnano system in-line connected to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) with a linear gradient reaching 55% MS solvent B after 145 min and 99% MS solvent B at 150 min ...
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bioRxiv - Genetics 2022Quote: Samples were suspended in 0.1% TFA and an equivalent to 1 µg of peptides was analyzed using Ultimate 3000 liquid chromatography system coupled to an Orbitrap QE HF (Thermo Fisher). An in-house packed analytical column (75 µm x 200 mm ...
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bioRxiv - Microbiology 2022Quote: ... 25 μg of peptides from each sample were labeled with 0.25 mg of the TMTpro TMT-labeling reagent (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Peptide LC-MS analysis was performed with a Dionex 3000 nano-HPLC coupled to an Orbitrap XL mass spectrometer (Thermo Fisher). Peptide samples were pressure-loaded onto a home-made C18 reverse-phase column (75 µm diameter ...
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bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
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bioRxiv - Cancer Biology 2022Quote: ... was determined by measuring the phosphorylation of a FRET peptide substrate (Ser/Thr19) labeled with Coumarin and Fluorescein using Z’-LYTE™ assay (Invitrogen). The 10 µL assay mixtures contained 50 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2019Quote: ... pull-down and sample preparation was performed as described (Wendrich et al., 2017).c Peptides were applied to online nano LCMS/MS (Thermo Scientific) using a 60 minute acetonitrile gradient from 5-50% ...
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bioRxiv - Molecular Biology 2019Quote: The purified digested peptides were separated with reverse phase liquid chromatography using a Dionex Ultimate 3000 RSLCnano system (Thermo Fisher Scientific) and analyzed by tandem mass spectrometry using Orbitrap Fusion mass spectrometry (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... MpPHOT proteins were detected with rabbit antibody raised against the custom-made synthetic peptide (C+VDERAPPSKGSAKE) (Eurofins Genomics) and horseradish peroxidase-conjugated anti-rabbit IgG (Thermo Scientific). Both antibodies were used at dilution of 1:2000 ...
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bioRxiv - Cell Biology 2019Quote: LC-MS/MS analyses of peptide mixture were done using RSLCnano system connected to Orbitrap Elite hybrid spectrometer (Thermo Fisher Scientific) with ABIRD (Active Background Ion Reduction Device ...
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bioRxiv - Biochemistry 2020Quote: ... Injected peptide samples were on-line de-salted/concentrated using an Acclaim PepMap C18 trap column (75 μm × 2 cm, Thermo Scientific) for 2 min at 5uL/min using 2% MeCN + 0.2% FA ...
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bioRxiv - Biophysics 2019Quote: ... Peptide amides were cleaved from the resin with 95% (v/v) aqueous trifluoroacetic acid solution (TFA, Fisher Scientific, Loughborough, United Kingdom) containing 5% (v/v ...