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Citations for Thermo Fisher :
4201 - 4250 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 2 μL of the peptide solution were chromatographed on a nano-capillary reverse phase column (Acclaim PepMap C18, 2 micron, 50 cm, Thermo Scientific) using a 0.1% formic acid/ 2% acetonitrile (Buffer A ...
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bioRxiv - Biochemistry 2020Quote: Individual fractions of the tryptic peptides were separated by a capillary C18 reverse phase column of the mass-spectrometer-linked Ultimate 3000 HPLC system (Thermo Fisher) using a 90 min gradient (5%-85% acetonitrile in water ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, 100Å, Thermo Scientific), before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
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bioRxiv - Cell Biology 2021Quote: ... then resuspended in 10 μL 50 mM HEPES pH 8.5 and quantified by Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific 23275).
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bioRxiv - Cell Biology 2021Quote: ... The pooled samples were then fractionated into 8 fractions using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific 84868), then dried by speed-vac ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody was purified from the immunized rabbit sera using affinity purification against the same peptide coupled to SulfoLink resin (Thermo-Fisher Scientific). The antibody was dialyzed into PBS+ 50% glycerol and stored with the addition of 0.05% sodium azide.
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bioRxiv - Cell Biology 2021Quote: ... Peptides were directly ionized using a nanospray ion source and analyzed on the Q-Exactive HF mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... tryptic peptides were injected into an Easy-nLC 100 HPLC system coupled to an Orbitrap Fusion Lumosmass spectrometer (Thermo Fisher Scientific). Specifically ...
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bioRxiv - Cell Biology 2021Quote: ... with reverse phase chromatography to separate peptides using an Ultimate 3000 RSLCnano system (Dionex) before analysis on a Thermo Orbitrap Fusion (Q-OT-qIT, Thermo Scientific). RAW files from LC-MS/MS were processed using MaxQuant version 1.5.3.8 or 1.6.7.0 (http://www.maxquant.org ...
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bioRxiv - Plant Biology 2020Quote: Synthetic Arabidopsis RALF1 polypeptide was synthesized by using 9-fluorenylmethyl chloroformate solid-phase chemistry with a peptide synthesizer from Thermo Scientific company and confirmed by MALD-TOF analysis (Applied Biosystems Voyager System 2098 ...
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bioRxiv - Plant Biology 2020Quote: ... Peptides were trapped to a reverse phase trap column (Acclaim PepMap C18, 5 μm, 100 μm × 2 cm, Thermo Fisher Scientific) connected to an analytical column (Acclaim PepMan 100 ...
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bioRxiv - Plant Biology 2020Quote: ... Eluted peptides were transferred to an NSI source and sprayed into an Orbitrap Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific). The MS was run in positive ion mode ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Systems Biology 2021Quote: ... Peptides were resolved on analytical column (75 μm × 50 cm, 3 μm particle, and 100 Å pore size; Thermo Fisher Scientific) at a flow rate of 300 nl/min over 120 min gradient in solvent B (80% Acetonitrile with 0.1% Formic acid (FA) ...
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bioRxiv - Microbiology 2020Quote: ... The specific modification of phosphor-Thr and phosphor-Tyr residues in the treated peptide was measured by tandem mass spectrometry (Thermo Scientific™ Orbitrap Fusion™ ...
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bioRxiv - Systems Biology 2021Quote: ... Dried peptides were resuspended in MS loading buffer (3% ACN, 0.1 % FA) and measured with Q Exactive HF (Thermo Fisher Scientific) coupled to a reverse-phase nano liquid chromatography ACQUITY UPLC M-Class system (Waters) ...
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bioRxiv - Systems Biology 2020Quote: ... of pTyr peptides were carried out on an Agilent 1260 LC coupled to a Q Exactive HF-X mass spectrometer (Thermo Fisher). Peptides were separated using a 140 min gradient with 70% acetonitrile in 0.2 M acetic acid at flow rate of 0.2 mL/min with approximate split flow at 20 nL/min ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg peptides from each fraction was loaded onto trap column nanoViper 2 cm (3 μm C18 Aq) (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (15 cm ...
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bioRxiv - Plant Biology 2021Quote: ... extracted peptides were separated on an EasyLC nano-HPLC coupled to a Q Exactive HF mass spectrometer (both Thermo Fisher Scientific) as described elsewhere (Kelstrup et al. ...
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bioRxiv - Microbiology 2021Quote: ... Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 RSLCnano and Q-Exactive HF, Thermo Scientific). Peptides were sampled on a 300 µm x 5 mm PepMap C18 precolumn and separated on a 75 µm x 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Microbiology 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Microbiology 2021Quote: The LC-MS/MS analysis of TMT-labelled peptides were performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) coupled with a Thermo Scientific Ultimate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitated proteins were eluted from beads with 0.2 ml PDE storage buffer containing 1 mg/ml HA-peptide (Thermo Fisher Scientific) with mixing at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... The application of a 3.5 kV distal voltage electrosprayed the eluting peptides directly into a Q Exactive HF mass spectrometer equipped with an EASY-Spray source (Thermo Scientific). Mass spectrometer-scanning functions and HPLC gradients were controlled by the Xcalibur data system (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... both preceded by a murine IgK leader peptide and followed by an 8xHis Tag were synthetized and cloned into pcDNA™3.1/Zeo(+) expression vector (Thermo Fisher). Proteins were produced by transient co-transfection of exponentially growing Freestyle™ 293-F suspension cells (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... The eluted peptides from the C18 column were analyzed using a Q-Exactive Plus Orbitrap MS (Thermo Scientific, San Jose, CA) for high resolution MS and high-energy collision-induced dissociation tandem MS by electrospray ionization ...
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bioRxiv - Plant Biology 2021Quote: ... the peptides were dissolved in 4 μL of 0.2% formic acid (FA) and injected into an Easy-nLC 1200 (Thermo Fisher Scientific). Peptides were separated on a 15 cm in-house packed column (360 μm OD × 75 μm ID ...
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bioRxiv - Plant Biology 2021Quote: ... Total peptide samples and the immuno-enriched fractions were then analyzed via LC-MS/MS using an EASY-nLC 1200 (Thermo Fisher) coupled to a Q Exactive HF mass spectrometer (Thermo Fisher) ...
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bioRxiv - Plant Biology 2020Quote: ... The lyophilized peptide was dissolved in nuclease-free water and its concentration determined on a Nanodrop 2000c (Thermo Scientific, Waltham, MA) using A280 with the molar extinction coefficient (3230 M-1cm-1 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Microbiology 2019Quote: Eluting peptides were converted to gas-phase ions by means of electrospray ionization and analysed on a Thermo Orbitrap Fusion (Thermo Scientific). Survey scans of peptide precursors from 375 to 1500 m/z were performed at 120K resolution (at 200 m/z ...
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bioRxiv - Microbiology 2020Quote: ... Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 and LTQ-Orbitrap Velos Pro, Thermo Scientific). Peptides were sampled on a 300 μm x 5 mm PepMap C18 precolumn and separated on a 75 μm x 250 mm C18 column (PepMap ...
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bioRxiv - Cell Biology 2021Quote: ... the peptides were resuspended in 3% formic acid (FA)/5% acetonitrile (ACN) and loaded onto a nanoLC system (RSLCnano, Thermo Scientific). First ...
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bioRxiv - Microbiology 2020Quote: ... Eluting peptide fragments were ionized by electrospray ionization and analyzed on a LTQ-XL Orbitrap Discovery hybrid instrument (Thermo Fisher Scientific). The LC–MS/MS procedure was performed according to the data-dependent acquisition (DDA ...
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bioRxiv - Microbiology 2020Quote: ... The experimental setup was as follows: the peptide mixture was loaded onto a 75 μm × 2 cm precolumn (Acclaim PepMap 100, C18, 3 μm, Thermo Scientific) and eluted over 60 min using a 75 μm × 25 cm analytical column (Acclaim PepMap RSLC ...
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bioRxiv - Cell Biology 2021Quote: Tryptic peptides were analysed using an EASY-nLC 1000 coupled to a Q Exac-tiveTM Hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific). The peptides were resolved and separated on a 50cm analytical EASY-Spray column equipped with pre-column over a 120 min gradient ranging from 8 to 38% of 0.1% formic acid in 95% acetonitrile/water at flowrate of 2 00nl/min ...
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bioRxiv - Cell Biology 2021Quote: ... Affinity enriched proteins were digested with trypsin and the resulting peptides desalted prior to LCMS analysis using an Easy-nLC 1000 system (Thermo Scientific) connected to a Q Exactive HF-X mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The peptides were trapped on a C18 PepMap 100 pre-column (300 μm i.d. x 5 mm, 100Å, Thermo Fisher Scientific) using solvent A (0.1% formic acid in water) ...
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bioRxiv - Cell Biology 2020Quote: The fractionated peptides were analysed by LC-MS/MS using a fully automated Ultimate 3000 RSLC nano System (Thermo Fisher Scientific) fitted with a 100 μm x 2 cm PepMap100 C18 nano trap column and a 75 μm×25 cm ...
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bioRxiv - Cancer Biology 2022Quote: ... The separated peptides were then ionized using a nanospray ionization ion source and analyzed using an Orbitrap Fusion™ (Thermo Scientific) mass spectrometer ...
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bioRxiv - Cancer Biology 2022Quote: ... The peptides were eluted from the pre-column onto an Easy-spray (25 cm x 75 μm; Thermo Fisher Scientific, DE) column into the Orbitrap Exploris 480 mass spectrometer ...
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bioRxiv - Cancer Biology 2022Quote: Tryptic peptides were analysed using an EASY-nLC 1000 coupled to a Q Exactive™ Hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific). The peptides were resolved and separated on a 50 cm analytical EASY-Spray column equipped with pre-column over a 120 min gradient ranging from 8 to 38% of 0.1% formic acid in 95% acetonitrile/water at a flow rate of 200nl/min ...
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bioRxiv - Biochemistry 2022Quote: ... Desalted peptides were solubilized in solvent A (0.1% aq. formic acid (FA)) and 1µg (determined by NanoDrop (Thermo Scientific Bremen, Germany)) was loaded and separated on an EASY-nLC (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... and then 1 μg of each peptide sample was loaded on a rap column (75 µm × 2 cm, 3 µm, C18, Thermo Fisher). The eluate was loaded onto a reversed-phase analytical column (50 µm × 250 mm ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide-RNA adducts were separated using a PepMap RSLC column (250 mm × 75 um, 2 µm particle size, Thermo Fisher Scientific) with gradient of 6-40% mobile phase B (A= water:ACN:FA ...
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bioRxiv - Biochemistry 2022Quote: ... Peptide-RNA adducts were separated on a PepMap RSLC column (150 mm × 75 um, 2 µm particle size, Thermo Fisher Scientific) using a gradient of 5-30% mobile phase B (A= water:ACN:FA ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... the peptides from each sample were reacted for 1 h with the appropriate tandem mass tag (TMT) isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested with trypsin (at a 1:50 trypsin to protein ratio) and resulting peptides were desalted on a C18 Hypersep column (Thermo Scientific) and dried down using a speed-vac ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested with trypsin (at a 1:50 trypsin to protein ratio) and resulting peptides were desalted on a C18 Hypersep column (Thermo Scientific) and dried down using a speed-vac ...