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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... RAP2.12 and SUB1A coding sequences in pENTR-D/TOPO (Invitrogen) were recombined into pDH51-GW-YFPc (Zhong et al. ...
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bioRxiv - Plant Biology 2024Quote: Full-length AtMED25 and OsMED25 coding sequences and the full-length AtMED25 promoter were cloned into pENTR-D/TOPO (Invitrogen) and recombined either into pK7WG2 (AtMED25 and OsMED25 coding sequences ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µL of a 1/10 dilution of the synthesized cDNA was mixed with PowerTrack SYBR Green Master Mix (Thermo Fisher Scientific, Waltham, MA) and primer pairs ...
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bioRxiv - Bioengineering 2024Quote: All qPCR reactions were performed in an Applied Biosystems QuantStudio 6 Flex using Applied Biosystems MicroAmp EnduraPlate Optical 384-well plates (Thermo Fisher Scientific, Waltham, MA, USA). Reactions were performed at 10 µL ...
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bioRxiv - Bioengineering 2024Quote: ... 1× proteinase and phosphatase inhibitor cocktail (#78440, Thermo Fisher Scientific) was added to the supernatant at 1:1 ratio ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue constructs were cultured for three weeks in chondrogenic medium (DMEM supplemented with 1% penicillin-streptomycin-glutamine, 1% HEPES (1M), 1% sodium pyruvate (100 mM), 1% ITS (100x) (Insulin, Transferrin, Selenium) (all from Gibco), 0,47 mg/ml linoleic acid (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: MSOD-B and MSOD-B ΔRUNX2 cells were trypsinized and washed with ice cold PBS twice and lysed on ice for 10 min in RIPA buffer (#10017003, Thermo Fisher Scientific) supplemented with 1× proteinase and phosphatase inhibitor cocktail (#78440 ...
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bioRxiv - Molecular Biology 2024Quote: ... Custom oligonucleotides were obtained from Thermo Fisher. T ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by Alexa Fluor 488 anti-rabbit IgG (Molecular Probes, Invitrogen). Cell nuclei were stained with DAPI DNA staining (300nM ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by Alexa Fluor 568 anti-mouse IgG (Molecular Probes, Invitrogen). Endogenous ERRα was visualized using rabbit anti-ERRα (ab137489 ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by Alexa Fluor 488 anti-rabbit IgG (Molecular Probes, Invitrogen). Cell nuclei were stained with DAPI DNA staining (300nM ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell nuclei were stained with DAPI DNA staining (300nM, Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... The concentration of RNA was measured at OD260/280 by a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Wilmington, Delaware). Reverse transcription was performed with miRNA P-RT Solution Mix and miRNA P-RT EnzymeMix (Sangon Biotech. ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg/ml TL TexasRed (Invitrogen) or 10 µg/ml hTf AF-647 (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... miRNA mimic (20 nM) and miRNA negative control (20 nM) were transfected into cells using Lipofectamine 2000 (Invitrogen, USA) according to the instructions of the reagent ...
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bioRxiv - Molecular Biology 2024Quote: ... One microgram of total RNA was treated with DNaseI (Thermo Fisher Scientific), and reverse transcribed with the Superscript III First Strand Synthesis Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycoproteins were resolved by SDS–PAGE (approx. 1×107 cell equivalents/lane) on NuPAGE bis-Tris 4–12% gradient acrylamide gels (Invitrogen) and transferred either to nitrocellulose or PVDF membranes (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... QPCRs were performed on a QuantStudio Real-Time PCR Light Cycler (Thermo Fisher Scientific).
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bioRxiv - Pathology 2024Quote: ... Total cholesterol was measured using the Infinity Cholesterol Reagent (Thermo Scientific: R22421).
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bioRxiv - Pathology 2024Quote: ... Tandem mass tag (TMT)-based labeling of peptides was performed using the TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific, Cat#90110) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestion was stopped by the addition of 2x STOP Buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 0.05% digitonin, 100 μg/ml RNase A (Thermo Fisher Scientific), 50 μg/ml glycogen ...
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bioRxiv - Molecular Biology 2024Quote: Drosophila Schneider 2 (S2) cells were purchased from Thermo Fisher Scientific and grown at 26 °C in Schneider media supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... regions around the CRISPR/Cas9 target sites were amplified via PCR using Phusion polymerase (ThermoFisher) and primers containing an eight-base-pair barcode (Supplementary figure 3) ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA concentrations were measured using the Qubit High Sensitivity kit (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: Isolated DNA was enriched for inversion events at both DSB sites in separate PCR reactions using Phusion polymerase (ThermoFisher). Per inversion DSB border ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.1% Pluronic F-68 (all from Gibco). Libraries were generated by triple transfection with 1 mg pAdDeltaF6 adenovirus helper plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... and RNA was reverse transcribed into complementary DNA (cDNA) using TaqMan™ Reverse Transcription Reagents (Thermo Fisher Scientific; N8080234). Total DNA was prepared with Superscript III First-Strand kit (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Total protein concentration for each sample was measured using a BCA Protein Assay kit (cat # 23225, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... A commercially available lactate dehydrogenase (LDH) kit was used to measure the release of LDH to the culture media (CyQUANT-ThermoFisher Scientific) according to the manufacturer’s description.
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% fetal calf serum and 0.2% P/S (Invitrogen). After formation of glia micro-islands ...
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bioRxiv - Neuroscience 2024Quote: ... Astrocytes were seeded onto these coverslips in DMEM (ThermoFisher Scientific), supplemented with 10% fetal calf serum and 0.2% P/S (Invitrogen) ...
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bioRxiv - Pathology 2024Quote: ... Total RNA was isolated using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... and slides were mounted using Prolong Glass Antifade Mountant (Thermo Scientific: P36984). Imaging was conducted on a Nikon Eclipse Ti-S microscope ...
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bioRxiv - Pathology 2024Quote: ... a donkey anti-rabbit Alexa Fluor 647 (Invitrogen: A-31573) and goat anti-mouse Alexa Fluor 546 (Invitrogen ...
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bioRxiv - Pathology 2024Quote: ... 500 µL of media (sorting media plus 1% ITS (Gibco, 41400-045)) was added to each well and incubated at 37°C in 5% CO2 for 14 days.
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bioRxiv - Pathology 2024Quote: ... Cyclohexane and chloroform were purchased from Thermo Fisher Scientific (Fitchburg ...
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bioRxiv - Pathology 2024Quote: ... respectively) were introduced into HC-Pro cistron by overlapping PCR using Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific) and appropriate primers (Table 1) ...
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bioRxiv - Pathology 2024Quote: ... Quantification was carried out using QuanBrowser module of Xcalibur software (Thermo Scientific) using peak area of m/z 136.0609 ...
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bioRxiv - Pathology 2024Quote: ... the two digested fragments were ligated by T4 DNA ligase (Thermo Fisher Scientific) to generate recombinant viruses harboring point mutation ...
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bioRxiv - Pathology 2024Quote: ... cDNA templates were then amplified in triplicates with SYBR Green PCR Master Mix using the QuantStudio3 real-time qPCR system (Applied Biosystems, Carlsbad, CA). The sequences for each primer set are provided in the Supplementary Document (Table S1).
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bioRxiv - Pathology 2024Quote: The samples were analyzed by online nanoflow liquid chromatography-tandem mass spectrometry utilizing a Q-Exactive mass spectrometer (Thermo-Fisher Scientific) coupled online to an Easy-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2024Quote: ... The 18 µl sample was loaded onto an Acclaim Pep Map 100 C18 trap column (3µm x 0.075mm x 20 mm; Thermo Fisher Scientific) and separated by a 12.5 cm capillary column NTCC-360/75-3-125 (C18 ...
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bioRxiv - Neuroscience 2024Quote: ... medium complemented with 2% Penicilin-Streptomycin (ThermoFisher Scientific), 0.5% Gentamycin (Merck) ...
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bioRxiv - Neuroscience 2024Quote: ... both retinas from either three (6-week-old) or two (12- or 18-week-old) fish were pooled and collected in Leibovitz 15 (L15, ThermoFisher Scientific) medium complemented with 2% Penicilin-Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Using sterile Dulbecco PBS (DPBS, ThermoFisher Scientific), the retinal pigment epithelial was rinsed off as much as possible ...
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bioRxiv - Neuroscience 2024Quote: ... and then mounted with ProLongTM Gold Antifade Mountant (ThermoFisher Scientific, CAT P10144) or Fluoroshield (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... 1:1000, goat, CG1, Swant, Burgdorf), Calbindin (CB, 1:1000, rabbit, CB38, Swant, Burgdorf) and eYFP (1:400, chicken, A10262, Thermo Fisher Scientific, Waltham, MS) and the matching secondaries for eYFP (goat anti chicken ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary incubations were conducted with donkey anti-mouse (1:200, A1037, Invitrogen, Waltham, MS) and donkey anti rabbit (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... and 100 units/ml of Penicillin-Streptomycin (Gibco, Life Tech., 15070063). Additionally ...
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bioRxiv - Neuroscience 2024Quote: ... and donkey anti rabbit (1:200, A1042, Invitrogen) antibodies ...