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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Dynabead Protein G beads (10004D, ThermoFisher) or anti-Flag M2 beads (M8823 ...
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bioRxiv - Biochemistry 2024Quote: ... BCA kit (Thermo Fisher Scientific, USA), high pH reverse peptide separation kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The next day cells were washed 3x with PBS and probed with secondary antibodies: 1:2000 Alexa Fluor® 488 Goat Anti-Rat IgG (Invitrogen, 10729174) or 1:1000 phalloidin (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... and 100 U/ml penicillin-streptomycin (Gibco, 11548876) in a humidified incubator at 37℃ and 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... oscillator (Thermo Fisher Scientific, USA), TS100 constant temperature mixer (Hangzhou Ruicheng Instruments Co ...
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bioRxiv - Bioengineering 2024Quote: ... in a derivative of pcDNA3.1(+) (Life Technologies, Carlsbad, CA).
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bioRxiv - Bioengineering 2024Quote: ... MOLT-4 cells were maintained at 37°C with 5% CO2 in suspension culture in a complete growth medium (Gibco’s RPMI 1640 with 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2024Quote: ... cells were thawed and resuspended in culture media consisting of the MEMα (Thermo Fisher Scientific) supplemented with KnockOut Serum Replacement (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... Slides were mounted under coverslip in Prolong Gold anti-fade reagent with DAPI (Life Technologies.) Stained sections were imaged using EVOS (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... 4.5 µg of random hexamers (ThermoFisher, Waltham, MA), and 10 U of DNA polymerase I (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were subject to 70% formamide (Invitrogen) in 2x saline-sodium citrate (SSC ...
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bioRxiv - Bioengineering 2024Quote: ... followed by a protease III (ACDbio) treatment at a dilution of 1:10 in PBS (Gibco) for 20 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were plated approximately at 1.5 × 105 cell/cm2 onto Vitronectin (Thermo Fisher Scientific)-coated glass or plastic surface ...
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bioRxiv - Bioengineering 2024Quote: ... The DNA concentration was assessed per manufacture protocol using Quant-iT PicoGreen dsDNA assay kit (Invitrogen, Carlsbad, CA). Fluorescence was measured using a microplate reader (FLUOstar Omega ...
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bioRxiv - Bioengineering 2024Quote: ... Stained sections were imaged using EVOS (Invitrogen, Thermo Fisher) and Zeiss LSM900 confocal imaging system ...
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bioRxiv - Bioengineering 2024Quote: ... and T1 (25 units/ml) (Thermo Scientific) in 1 × Tris-buffered saline containing 0.05% Tween-20 (TBS-T ...
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bioRxiv - Bioengineering 2024Quote: Reactive oxygen species (ROS) were quantitatively and qualitatively determined using the cell permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (ThermoFisher Scientific). H2DCFDA is a type of fluorescein that has been chemically reduced and is used to detect the generation of reactive oxygen intermediates in neutrophils and macrophages ...
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bioRxiv - Bioengineering 2024Quote: ... or directly purchased from Fisher Scientific (Gibco™ PBS ...
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bioRxiv - Bioengineering 2024Quote: ... iPSCs were harvested using Accutase (Gibco; A1110501), seeded on iMatrix-511 silk (Elixirgen Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... 150ng of DNA was run on 3 lanes of a 1% eGel (Thermo Fisher Scientific, Waltham, MA) and size-selected into two populations ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were injected for LC-MS/MS analysis with Q Exactive HF mass spectrometer (ThermoFisher, Waltham, MA) coupled with Evosep One (Evosep ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% FBS (#16000-044, Thermo Fisher Scientific, Waltham, MA) and penicillin-streptomycin (100 μg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... UltraComp eBeads™ compensation beads (Fisher Scientific; 01-2222-41) stained with the same secondary antibody were used as a positive control for analysis ...
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bioRxiv - Bioengineering 2024Quote: HCT116LMN(B1&B2)-AID cells and U2OS cells were grown in McCoy’s 5A Modified Medium (#16600-082, Thermo Fisher Scientific, Waltham, MA) supplemented with 10% FBS (#16000-044 ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor™ 488 (Invitrogen; A-21202) for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... the HLOs were treated with 35 µL of Visikol® Histo-M™ in a regular 384-well plate (ThermoFisher Scientific; 242757) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... 100 nM) of each FP in the presence of an oxygen scavenger system (OSS) consisting of 1 mM Trolox (Fisher Scientific, Cat. No. AC218940050), 5 mM 3,4-Dihydroxybenzoic acid (Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... ExpiCHO-S cells were grown to a density of 6 x 106 cells/mL and transfected using the ExpiFectamine CHO Transfection Kit (ThermoFisher Scientific) and expression carried out for 7-10 days post-transfection at 37°C with 8% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... and the YAP/TAZ-TRE-tdTomato plasmid using Lipofectamine 2000 (Invitrogen). Cells were cultured for 2 days post-transfection ...
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bioRxiv - Biochemistry 2024Quote: ... Expi293F cells were grown to a density of 3 x 106 cells/mL and transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and expression carried out for 3 to 5 days post-transfection at 37°C with 8% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... S or the S357A mutant S plasmids were prepared in 1.5mL of OPTI-MEM and combined with 60µL of Lipofectamine 2000 (Life Technologies) diluted in 1.5mL of OPTI-MEM and incubated at room temperature for 15-20 min ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed with OPTI-MEM (Life Technologies). 24µg of either the wildtype HKU1 isolate N1 (genotype A ...
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bioRxiv - Biochemistry 2024Quote: ... followed by goat anti-rabbit IgG-Alexa 647 (1:1,000; ThermoFisher Scientific) for 30 min and analyzed by flow cytometry.
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bioRxiv - Bioengineering 2024Quote: Ptk2 cells were grown in Eagle’s Minimum Essential medium (ATCC, 30-2003) supplemented with 10% Fetal bovine serum (Gibco, 26140079), in a 5% CO2 incubator ...
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bioRxiv - Bioengineering 2024Quote: ... a voltage-sensitive dye60 (FluoVolt, ThermoFisher) was bath-loaded using a peristaltic pump ...
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bioRxiv - Bioengineering 2024Quote: ... Cell cultures were then diluted 1:1000 in 1mL of 2% LB media pre-mixed with 0.1ug/mL FM1-43FX membrane stain (Life Technologies F35355). 50uL of the stained cell culture was then loaded into a transparent FEP tube and sealed for imaging.
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bioRxiv - Bioengineering 2024Quote: ... and penicillin–streptomycin (0.4%) from Gibco; and ITS + Premix (1% ...
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bioRxiv - Bioengineering 2024Quote: ... Polypropylene 2 mL deep 96-well blocks and PhyNexus™ C18 phytip® columns were purchased from Fisher Scientific™ ...
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bioRxiv - Bioengineering 2024Quote: ... RNA concentration was quantified using a Qubit 4 Fluorometer (Thermo Fisher), and RNA integrity numbers (RINs ...
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bioRxiv - Bioengineering 2024Quote: ... and cDNA was synthesized with a commercially available kit (High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, Thermo Fisher).
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bioRxiv - Bioengineering 2024Quote: ... and/or Hoechst (16 µM, Invitrogen) were added to the well ...
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bioRxiv - Bioengineering 2024Quote: ... The gene-specific primers mentioned in Supplementary Table 1 were used in PCR reactions per the manufacturer’s instructions using an Applied Biosystems (Life Technologies, Carlsbad, CA) real-time PCR system ...
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bioRxiv - Bioengineering 2024Quote: ... SYTOX Green (1/50,000; Invitrogen), and/or Hoechst (16 µM ...
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bioRxiv - Bioengineering 2024Quote: TRIzol reagent (Invitrogen, USA) was used to isolate total RNA from 15-day-old Arabidopsis seedlings ...
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bioRxiv - Biochemistry 2024Quote: ... frozen high-speed centrifuge (Thermo Fisher Scientific, USA), vacuum concentrator (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: HeLa cells transfected with GFP proteins were grown in a 96-well optical plate (Thermo Fisher Scientific) or 24-well lidded plates ...
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bioRxiv - Biochemistry 2024Quote: ... Purified water for mass spectrometry (Thermo Fisher Scientific, USA), Tris-Base (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The protein concentration was determined in a 96-well format using the Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant after centrifugation was applied to a gravity-flow immobilized metal ion affinity chromatography (IMAC) column (HisPur cobalt resin, ThermoFisher). The column was washed with buffer B (50 mM Tris/HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... Transfection was performed using Lipofectamine RNAiMax (Invitrogen) according to the manufacturer’s instructions ...