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Citations for Thermo Fisher :
3351 - 3400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Cells were harvested by treating 0.05 % Trypsin/EDTA (25300062, Gibco), and washed with Dulbecco’s modified eagle medium (DMEM ...
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bioRxiv - Physiology 2024Quote: ... and washed with Dulbecco’s modified eagle medium (DMEM, 12430054, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2024Quote: ... All reactions were run on a QuantStudio platform (Fisher Scientific).
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bioRxiv - Physiology 2024Quote: ... unstained paraffin tissue sections were de-paraffinized using xylene (UN1307, Fisher scientific) followed by rehydration with different % of ethanol (From 100 % to 70 % ...
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bioRxiv - Physiology 2024Quote: ... The signal was detected using Super SignalTM West Pico PlUS Chemiluminescent Substrate (34577, Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... A reverse transcription was carried out using 2.5 μg of RNA following the instructions provided with the kit (Cat Nº, 11766050, Invitrogen). For PCR reaction mix preparation ...
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bioRxiv - Physiology 2024Quote: ... Total RNA Microarray analysis was performed with the Affymetrix GeneChip Human Transcriptome Array 2.0 (Thermo Fisher Scientific, MA). Metabolomic analysis was performed by Metabolon (Durham ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative real-time PCR assays were performed in PowerUp SYBR Green Master Mix (Applied Biosystems) using a QuantStudio 3 Real Time PCR system under the following sequence of conditions ...
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bioRxiv - Immunology 2024Quote: ... Data acquisition was controlled by Xcalibur (version 4.4.16.14, Thermo Fisher Scientific).
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bioRxiv - Physiology 2024Quote: ... and 0.02% Pluronic F-127 (Invitrogen P6867). Cells were washed and left to recover for 30 min before use ...
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bioRxiv - Neuroscience 2024Quote: ... using the Amplex Red Hydrogen Peroxide Kit (Invitrogen). Flies (10/genotype/replicate ...
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bioRxiv - Physiology 2024Quote: Mouse DRG neuron cultures were loaded for 30 min at 37 °C with 3 µM Fura-2 AM (Invitrogen F1221) in ES containing also 0.02% Pluronic F-127 and left to recover for about 10 min in ES before recording ...
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bioRxiv - Physiology 2024Quote: ... Coverslips with attached cells were incubated for 30 min at 37 °C in ES containing 2 µM Calcium Green-1 AM (Invitrogen C3011MP, Thermo Fisher Scientific) and 0.02% Pluronic F-127 (Invitrogen P6867) ...
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bioRxiv - Physiology 2024Quote: ... Coverslips with attached cells were incubated for 30 min at 37 °C in ES containing 2 µM Calcium Green-1 AM (Invitrogen C3011MP, Thermo Fisher Scientific) and 0.02% Pluronic F-127 (Invitrogen P6867) ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies conjugated with Alexa fluorophores (Life Technologies) were diluted 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... A 20 µL aliquot of each sample was placed in a tube and fed into an autosampler of a Dionex Ultimate 3000 HPLC system at 5 ⁰C (Thermo Fisher Scientific, Waltham, MA, USA). External standards for DA ...
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bioRxiv - Plant Biology 2024Quote: ... first strand cDNA synthesis was performed from 2µg of total RNA using SuperScript IV VILO (ThermoFisher, Cat. No. 11756050). Relative transcript abundance was measured using the SYBR Green Master Mix (POWER SYBR GREEN PCR ...
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bioRxiv - Plant Biology 2024Quote: ... Lysates were cleared by centrifugation at (21,000 g, 5 min, 4 °C) and endogenous metabolites were removed with Zeba Spin Desalting Columns (ThermoFisher, cat. no 89882). The lysate was then split into sample replicates for PISA (four replicates for each treatment) ...
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bioRxiv - Plant Biology 2024Quote: ... qPCR was performed using SYBR™ Green PCR Master Mix (Applied Biosystems) on a 7500 Fast Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: Standards and samples were injected (10 µL) by an autoinjector using an acetonitrile based MD-TM mobile phase (10% acetonitrile: Thermo Fisher Scientific). HPLC flow rate was 0.6 mL / min ...
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bioRxiv - Plant Biology 2024Quote: ... First strand cDNA synthesis from 1 µg DNA-free RNA was carried out using the RevertAid H Minus reverse transcriptase with oligo(dT)18 primers (ThermoFisher Scientific™, www.thermofisher.com). Quantitative PCR was carried-out in Hard-Shell® 96-Well PCR Plates (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... and a column guard (2.1/3.0 mm ID, Thermo Fisher Scientific). For electrochemical detection ...
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bioRxiv - Neuroscience 2024Quote: ... Samples passed through a reverse-phase column (HypersilTM BDS C18 column, Thermo Fisher Scientifc) at 25 ⁰C ...
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bioRxiv - Plant Biology 2024Quote: ... on a 7500 Fast Real-Time PCR system (Applied Biosystems) according to the user’s manual ...
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bioRxiv - Neuroscience 2024Quote: ... Detector values were captured using Chromileon 7 software (Dionex, Thermo Fisher Scientific) to quantify peak height ...
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bioRxiv - Plant Biology 2024Quote: ... 12.5 µg of each sample was resuspended in 20 µL 20 mM EPPS buffer pH 8.5 and labeled with 0.1 mg TMTpro™ 16plex (Thermo Scientific, cat. no. A44521) for 1 hour at 600 rpm ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Plant Biology 2024Quote: ... and treated with the DNA-free™ DNA Removal Kit (Invitrogen, #AM1906). First strand cDNA synthesis from 1 µg DNA-free RNA was carried out using the RevertAid H Minus reverse transcriptase with oligo(dT)18 primers (ThermoFisher Scientific™ ...
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bioRxiv - Plant Biology 2024Quote: ... The JMJ13 catalytic domain was amplified with the primers listed in Table S1 and cloned into SunTag dCas9 plasmid using the BsiWI restriction enzyme (Thermo Scientific™, ER0851). The final construct allows to produce (i ...
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bioRxiv - Neuroscience 2024Quote: ... ERCC spike-in mixes (Cat Nº 4456739, ThermoFisher) were added to all samples to facilitate data normalization and quantification ...
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bioRxiv - Plant Biology 2024Quote: Frozen chloroplast aliquots were thawed on ice and NP-40 Surfact-Amps™ Detergent Solution (ThermoFisher, cat. no 13444269) was added to 0.8 % ...
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bioRxiv - Plant Biology 2024Quote: ... After DNase treatment (ezDNase SuperScript IV VILO, ThermoFisher, Cat. No. 11756050), first strand cDNA synthesis was performed from 2µg of total RNA using SuperScript IV VILO (ThermoFisher ...
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bioRxiv - Plant Biology 2024Quote: ... The isolated RNA was used for cDNA preparation using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2024Quote: ... Relative transcript abundance was measured using the SYBR Green Master Mix (POWER SYBR GREEN PCR, Thermo Fisher Scientific, 10658255) on a CFX Connect BioRad Real-Time PCR System ...
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bioRxiv - Plant Biology 2024Quote: ... Labeled peptides were separated into 6 fractions with Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermofisher, Cat. no. 84868) and dried in a Speed Vac at 45 °C for 3.5 hours ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were digested overnight (16 hours, 600 RPM) with Trypsin/Lys-C Protease Mix (Thermo Scientific, cat. No. A40009) at a 1:50 enzyme:protein ratio ...
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bioRxiv - Plant Biology 2024Quote: ... protoplasts were labelled with 300 nM final concentration of fluorescent probe di-4-ANEPPDHQ (Invitrogen, stock solution 1.5 mM in DMSO) in protoplast storage solution ...
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bioRxiv - Physiology 2024Quote: ... and quantified by the ViiA 7 Real-Time PCR System (Applied Biosystems, USA). The amount of FoxO1 and FoxO3 mRNA expression was normalized with endogenous control TFRH ...
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bioRxiv - Neuroscience 2024Quote: ... All electroporated plasmids were purified with an endotoxin-free maxi kit (Invitrogen) and dissolved in sterile PBS 1X containing 0.025% Fast Green (SIGMA) ...
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bioRxiv - Pathology 2024Quote: ... and protein concentration was measured using a BCA kit (Thermo Fisher, #23225) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the optical density was read at 490 nm using the Multiskan microplate spectrophotometer (ThermoFisher Scientific, Waltham, MA, USA). Relative MUC protein expression was normalized to the number of cells in the well following each treatment.
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bioRxiv - Pharmacology and Toxicology 2024Quote: The number of cells in each of the 24-well plate following DON treatment was first determined by trypan blue (Gibco) exclusion assay for calculation of MOI ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Osteoblasts were maintained in alpha-minimal essential medium (aMEM) (Gibco) containing 10% FBS (Gibco) ...
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bioRxiv - Pathology 2024Quote: Each sample was labelled with the TMTpro 18plex reagent set (Lot:XJ351218 and XK350589, Thermo Scientific) according to the manufacturer’s instructions utilising a singular reference channel (126 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... containing 10% FBS (Gibco), 2mM L-glutamine (Corning) ...
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bioRxiv - Pathology 2024Quote: ... The samples were loaded onto in Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific) and separated on an Acclaim PepMap RSLC (75 μm x 50 cm ...
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bioRxiv - Pathology 2024Quote: The raw data files were analyzed using Sequest within Proteome Discoverer (v2.5.0.400, Thermo Scientific)□to obtain protein identifications and their respective reporter ion intensities using in-house standard parameters ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... foetal bovine serum (FBS) and all other cell culture reagents were obtained from Gibco-Life Technology (Eggenstein ...
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bioRxiv - Neuroscience 2024Quote: ... anti-H3K9M (Invitrogen, MA5-33389 1:1000), anti-H3K27M (Cell Signaling Technology ...