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Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Protein content of the crude mitochondrial fraction was measured by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific catalog number 23225). Thirty µg of mitochondria protein was incubated at 25 °C in 850 µL reaction buffer (100 mM Tris-HCl ...
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bioRxiv - Plant Biology 2024Quote: ... Protein quantification of the extracts was estimated using the BCA Protein Assay Pierce (Thermo Scientific) and bovine serum albumin as standard.
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Mutations in HUA2 restore flowering in the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutantbioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized from 3 µg total RNA in a 10 µl reaction volume using the RevertAid Premium First Strand cDNA Synthesis Kit (Fermentas, Thermo Fisher Scientific, Rochester, NY). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Plant Biology 2024Quote: Constructs were cloned into the vector pIX-HALO (obtained from Arabidopsis Biological Resource Center) in an LR Clonase reaction (ThermoFisher), and checked using Sanger sequencing by GENEWIZ (Azenta) ...
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bioRxiv - Plant Biology 2024Quote: ... Immunolabelling experiments were performed with LM19 and LM20 (1:10 dilution in TTBS-milk, 2 h incubation) followed by anti-rat IgG-Alexa488 (Invitrogen®) or anti-rat IgG alkaline phosphatase conjugate (Sigma-Aldrich A8438 ...
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bioRxiv - Plant Biology 2024Quote: ... Amplification of the rbcL gene fragments was performed in a Veriti 96-well Thermal Cycler (Applied Biosystems) with the primer pair rbcLF (5′-AAAGCNGGKGTWAAAGAYTA −3′ ...
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bioRxiv - Plant Biology 2024Quote: ... thaliana Col-0 genomic DNA using the AccuPrime™ Taq DNA Polymerase High Fidelity (Invitrogen®, Carlsbad, CA, USA). The primer sequences are given in Supplementary Table S1 ...
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bioRxiv - Plant Biology 2024Quote: ... The integrity and quality of the total RNA were checked using NanoDrop 2000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA) and formaldehyde agarose gel electrophoresis ...
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bioRxiv - Bioengineering 2024Quote: ... the tissue constructs were rinsed twice with PBS 7.2 (without calcium/magnesium Gibco), snap frozen in liquid nitrogen for five minutes and then lyophilized using a freeze dryer (Labconco ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 1× proteinase and phosphatase inhibitor cocktail (#78440, Thermo Fisher Scientific). The lysates were centrifuged at 16 ...
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bioRxiv - Bioengineering 2024Quote: MSOD-B cells and their modified progeny were harvested from culture flasks by adding Trypsin-EDTA (0.25%) (Gibco) and subsequently seeded on cylindrical Collagen Type I scaffold (AviteneTM UltrafoamTM Collagen Sponge ...
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bioRxiv - Plant Biology 2024Quote: ... One μg of RNA was used for a 20-µL first-strand cDNA synthesis reaction using High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA) following the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2024Quote: ... The absorbance was then measured at 390 nm using the spectrophotometer GENESYS 20 (Thermo Fisher Scientific, Waltham, MA) after the reaction rested for 1h in darkness ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μl of the PCR product was loaded onto a 1.5% agarose gel stained with ethidium bromide (Invitrogen, Carlsbad, CA). The electrophoresis was conducted at 100 V for 40 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... the fragmented mRNA was reverse transcribed to create the first strand of cDNA with random hexamers and SuperScript™ II Reverse Transcriptase (Thermo Fisher Scientific) followed by second strand synthesis ...
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bioRxiv - Plant Biology 2024Quote: ... using Phusion DNA Polymerase (Thermo Scientific). To generate pENTR pVIN3::VIN3-GFP VRN5VEL ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were washed with TBS containing 0.05% Tween-20 and developed with SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific).
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bioRxiv - Synthetic Biology 2024Quote: ... 10 mM MgCl2) with a Pierce protease inhibitor tablet (Thermo Fisher). The cell suspension was lysed by sonication and the lysate was clarified by centrifugation at 14,000 rpm (34,000 xg ...
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bioRxiv - Plant Biology 2024Quote: ... then transcribed into cDNA with SuperScript Reverse Transcriptase IV (both Life Technologies) with gene-specific reverse primers (Supplemental Table S1) ...
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bioRxiv - Plant Biology 2024Quote: ... or goat anti-rabbit IgG (H+L) secondary antibody (1:5000 dilution, Invitrogen, USA) for one hour ...
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bioRxiv - Plant Biology 2024Quote: The pENTR-constructs were transferred to the binary vector pSLJ-DEST (based on pSLJ755I6) with L/R reaction (Invitrogen) and then transformed into vin3-1 FRI or vrn5-8 FRI mutants mediated by Agrobacterium C58 using the floral dip method ...
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bioRxiv - Plant Biology 2024Quote: Total RNA (1μg) was extracted from whole seven-day-old seedling tissue using TRIzol reagent (Invitrogen) and treated with the TURBO DNA-free kit (Ambion ...
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bioRxiv - Plant Biology 2024Quote: ... and treated with the TURBO DNA-free kit (Ambion) to remove residual genomic DNA ...
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bioRxiv - Plant Biology 2024Quote: ... The latter vectors were used in a Gateway LR cloning (Gateway® LR ClonaseTM II Plus Enzyme Mix, Invitrogen; Cat#:12538-120) with pEarleyGate100 (76 ...
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bioRxiv - Plant Biology 2024Quote: ... These sequences were then recombined with pENTRTM/D-TOPO (Invitrogen, Cat#45-0218) to yield pTOPO_PSY1 and pTOPO_ProPSY1 ...
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bioRxiv - Plant Biology 2024Quote: ... The concentration of total RNA was measured by a Qubit (Invitrogen) instrument ...
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bioRxiv - Pathology 2024Quote: Liquid chromatography-mass spectrometric (LC-MS) analysis was conducted using an□Orbitrap Eclipse Tribrid mass spectrometer (Thermo Scientific, Breman, Germany) and Nano LC system (Dionex Ultimate 3000 RSLCnano) ...
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bioRxiv - Synthetic Biology 2024Quote: Kinetic constants of SrIRED for cyclohexanone were determined in 100 mM Tris pH 8.0 with 0.5 mM NADPH and varying concentrations of cyclohexanone (0.06 mM - 35 mM) in transparent Nunc 96-well plates (ThermoFisher) and by monitoring the depletion of NADPH concentration at λ of 340nm (Spectramax 190 ...
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bioRxiv - Plant Biology 2024Quote: ... followed by the insertion of a 10 amino acid linker between VRN5 and SYFP2 with Quickchange PCR using Phusion DNA Polymerase (Thermo Scientific). This wild-type construct was modified by Quickchange ...
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bioRxiv - Plant Biology 2024Quote: ... sample concentration was determined by Qubit flourometer (Invitrogen) using the Qubit HS RNA assay kit ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cell suspension was lysed by sonication and the lysate was clarified by centrifugation at 14,000 rpm (34,000 xg) for 45 min in a F14-14 × 50cy rotor (ThermoFisher). The clarified lysate was then loaded onto a sucrose cushion with 24 mL of buffer B (20 mM Tris–HCl pH 7.5 ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... Slides were treated with 20 U of Micrococcal nuclease enzyme (MNase; Thermo Fisher, Waltham, MA, USA) for 30 min at 37°C to remove external fluorescein-labelled dsRNAs (Qiao et al. ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... 1999).1 µL of the extracted DNA was used in 20 µL reactions of Phusion™ High-Fidelity DNA Polymerase (Thermo Fisher, Waltham, MA, USA) containing 0.4 µL of 1:1000 SYBR™ Green I Nucleic Acid Gel Stain (Thermo Fisher ...
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bioRxiv - Plant Biology 2024Quote: ... 10 kDa MWCO (PES membrane, 5 20 mL, Thermo Fisher Scientific). Finally ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... in x1 DNase buffer (Thermo Fisher, Waltham, MA, USA). Enzymes were removed using the RNA Clean & Concentrator Kit (Zymo Research ...
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bioRxiv - Plant Biology 2024Quote: ... the extracted RNA underwent treatment with the TURBO DNA-free™ Kit (Invitrogen, Carlsbad, CA, United States). The concentration of RNA was adjusted to 200 ng/μl ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... Fluorescein-labeled dsRNA was synthesized using 2 µL of the plasmid pT777T-YFP in 20 µL reactions of the MEGAscriptTM RNAi Kit (Thermo Fisher, Waltham, MA, USA) with 2 µL of the Fluorescein labeling mix (Roche ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... Amplifications were carried in the QuantStudio 6 Flex Real-time PCR System (Applied Biosystems) using a protocol consisting of an initial activation step at 94°C for 2 min 30 sec ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... and 1µL RNase T1 (1000 U/µl) (Thermo Fisher, Waltham, MA, USA) in x1 DNase buffer (Thermo Fisher ...
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bioRxiv - Plant Biology 2024Quote: ... 10 kDa MWCO (PES membrane, 20 mL, Thermo Fisher Scientific). FtsZ1-2 and FtsZ2-1 yields after purification were quantified via densitometry after SDS-PAGE using BSA as protein standard ...
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bioRxiv - Plant Biology 2024Quote: ... BL21 Star™ (DE3) One Shot® (Invitrogen, Thermo Fisher Scientific) cells were transformed with the respective vectors and the transformed cells were used to inoculate 200 mL LB medium containing 50 mg/ml kanamycin and 0.025 % glucose ...
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bioRxiv - Biochemistry 2024Quote: ... followed by 2 uL SuperScript II (Invitrogen). Reactions were incubated at 25°C for 10 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... 50 µL of the diluted sample was dispensed into 96-well microplates (Nunc-Roskilde, Denmark) and allowed to incubate overnight at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... Using SYBR Green master mix and QuantStudio 5 (Applied Biosystems), qPCR was run with 5 min at 95°C ...
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bioRxiv - Plant Biology 2024Quote: ... Alcohol Insoluble Materials (AIMs) were extracted from 20 to 30 mg of lyophilised root tissues using Dionex™ ASE™ 350 accelerated solvent extractor (Thermo Fisher Scientific Inc., Waltham, MA, USA). The identification and quantification of cell wall neutral sugars were performed by gas-chromatography (Trace GC Ultra ...
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bioRxiv - Plant Biology 2024Quote: ... Real-time quantitative PCR (qPCR) was performed on a QuantStudio6 apparatus (Applied Biosystems®, Carlsbad, CA, USA) using Takyon™ reagents (Eurogentec ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentration was checked using Pierce BCA protein assay kit (Thermo Scientific, USA) and typically 100 µg protein was loaded onto 10% SDS-PAGE gels ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized with Superscript III reverse transcriptase (Invitrogen®) using 1 µg of total RNA ...
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bioRxiv - Biochemistry 2024Quote: ... 8 µL of 2.5X MaP Buffer was added and incubated at 42°C for 2 minutes before addition of 1 µL SuperScript II Reverse Transcriptase (ThermoFisher) and incubation at 42°C for 3 hours ...
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bioRxiv - Plant Biology 2024Quote: ... samples were dried using a SPD111 SpeedVac (Thermo Scientific) until complete evaporation ...