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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and all data were collected using a Talos Arctica microscope (ThermoFisher) operating at 200kV equipped with a K3 direct electron detector (Gatan) ...
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bioRxiv - Bioengineering 2024Quote: ... calcein stain was purchased from Invitrogen. Calcium quantification kit was purchased from Stanbio ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue culture reagents were obtained from GIBCO, ATCC ...
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bioRxiv - Bioengineering 2024Quote: ... and penicillin-streptomycin (100 μg/ml; #15140-122, Thermo Fisher Scientific, Waltham, MA). To create these cells ...
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bioRxiv - Bioengineering 2024Quote: ... cDNA was synthesized from 1 µg of RNA by following the protocol of the high-capacity cDNA reverse transcription kit (Applied Biosystems; 4368814). Real time PCR was performed using PowerTrackTM SYBR green master mix (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... The secondary antibody goat anti-rabbit AF647 (Thermo Fisher Scientific, #A-21245, dilution 1:1000) was stored at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor Plus 647 (Thermo Fisher Scientific, #A32728, dilution 1:200). Cells were washed with 1x PBS 3 times for 5 minutes each ...
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bioRxiv - Bioengineering 2024Quote: ... 10 ng/mL recombinant human VEGF-165 (Gibco; PHC9391), 20 ng/mL recombinant human EGF (R&D system ...
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bioRxiv - Bioengineering 2024Quote: ... Real time PCR was performed using PowerTrackTM SYBR green master mix (Applied Biosystems; A46110) and forward/reverse primers from IDT Technology in the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed twice with 1x Phosphate Buffered Saline (PBS) (Gibco, #10010031). Cells were fixed with 4% paraformaldehyde (PFA ...
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bioRxiv - Bioengineering 2024Quote: ... The HLOs were fixed using 4% paraformaldehyde (PFA; ThermoFisher Scientific; J19943K2) for 2 hours at room temperature while gently rocking ...
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bioRxiv - Bioengineering 2024Quote: ... 2 μg/ml of doxycycline (Fisher Scientific, #10592-13-9) was added to cells 24 hours prior to auxin treatment ...
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bioRxiv - Bioengineering 2024Quote: ... Exp-HLOs were enzymatically dissociated into small clumps by treatment with 1x TrypLE™ Express Enzyme (ThermoFisher; 12605010) for 5 minutes at 37°C in the CO2 incubator ...
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bioRxiv - Bioengineering 2024Quote: ... the HLOs were transferred to a microscope cover glass (Fisher Scientific; 22266882) and treated with 25 μL of Visikol® Histo-M™ (Visikol ...
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bioRxiv - Bioengineering 2024Quote: ... The HLOs were stained with 500 µL of 0.5 µg/mL DAPI solution (ThermoFisher Scientific; 62248) in 1x dPBS-/- for 30 minutes at room temperature with gentle rocking ...
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bioRxiv - Bioengineering 2024Quote: ... The cell clumps in Matrigel dome were cultured in the EM supplemented with 25 ng/mL human active BMP7 recombinant protein (Gibco; PHC7204) initially for 3 - 6 days to regenerate and form Exp-HLOs using BMP7 ...
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bioRxiv - Bioengineering 2024Quote: ... Transfection was then performed using Lipofectamine LTX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... 1X non-essential amino acids (Gibco), 100 U/ml penicillin (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... cells were stained with DAPI (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... and forward/reverse primers from IDT Technology in the QuantStudio™ 5 Real-Time PCR System (Applied Biosystems; A28574). The cycle was run 40 times at 95°C denaturation for 30 sec ...
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bioRxiv - Bioengineering 2024Quote: ... was solubilized in RNase-free water (Fisher Scientific, #10-977-015) before each treatment as a fresh solution and added to HCT116LMN(B1&B2)-AID cells.
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bioRxiv - Bioengineering 2024Quote: ... 1% (vol/vol) penicillin-streptomycin (Gibco), 1% (vol/vol ...
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bioRxiv - Bioengineering 2024Quote: ... medium was supplemented with 0.1% penicillin/streptomycin (Gibco).
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bioRxiv - Bioengineering 2024Quote: ... 2% (vol/vol) GlutaMAX (Gibco), 1% (vol/vol ...
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bioRxiv - Bioengineering 2024Quote: ... 1% (vol/vol) non-essential amino acids (Gibco), 1% (vol/vol ...
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bioRxiv - Bioengineering 2024Quote: ... U87 cells were cultured in fully supplemented high-glucose Dulbecco’s modified Eagle medium (DMEM, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Bioengineering 2024Quote: Anti-elastase activity was determined using the EnzChek™ Elastase Assay kit (Invitrogen™). A 1.5-fold dilution series of A1AT was prepared ...
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bioRxiv - Bioengineering 2024Quote: ... and 1% (vol/vol) penicillin-streptomycin (Gibco).
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bioRxiv - Bioengineering 2024Quote: ... and 1% (vol/vol) MEM non-essential ammino acids solution (Gibco). U87 cells were transfected with cytosolic green fluorescent protein (GFP ...
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bioRxiv - Bioengineering 2024Quote: ... devices were submerged in phenol-free serum-free DMEM (Gibco) overnight ...
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bioRxiv - Bioengineering 2024Quote: ... where sulfate-modified (580/605) 1 μm sized FluoSpheresTM (Thermo Fisher, F8851) were mixed into the hydrogel solution prior to crosslinking ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was isolated with TRIzol Reagent (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer’s protocol and sent to Macrogen Japan Corp ...
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bioRxiv - Bioengineering 2024Quote: ... HAMe or HAMe-RGD were crosslinked in phenol-free serum-free DMEM (Gibco) with di-thiol peptide crosslinkers that were either broadly protease-degradable (KKCGGPQGIWGQGCKK ...
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bioRxiv - Bioengineering 2024Quote: ... GSC-295 cells were propagated as neurospheres in DMEM/F12 basal medium supplemented with 2% (vol/vol) B-27 supplement (Gibco), 20 ng/mL EGF (R&D Systems) ...
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bioRxiv - Bioengineering 2024Quote: ... Slices were affixed to the petri dishes using 0.1% agarose (Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... The qPCRs were performed using Power SYBR Green PCR Master Mix (Applied Biosystems) and primers (Table SE4 ...
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bioRxiv - Bioengineering 2024Quote: ... 1% (vol/vol) sodium pyruvate (Gibco), and 1% (vol/vol ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were sonicated on ice at 40% amplitude for one hour by a 120 W ultrasonicator with a 1/8” microtip probe (Fisher Scientific; Hampton, NH). Sonicated suspensions were ultracentrifuged at 58,000 x g for one hour using an Optima Max-XP Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Bioengineering 2024Quote: ... DAPI (NucBlue Fixed Cell ReadyProbes Reagent; Thermo Fisher Scientific, Waltham, MA) was added during the last washing step and sections were covered with Fluoromount-GT (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Coverslips and films were then mounted on glass slides for imaging using Fluoromount mounting medium (Invitrogen, UK).
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bioRxiv - Bioengineering 2024Quote: ... a LIVE/DEAD Cell Imaging Kit (ThermoFisher) was used to fluoresce live cells (ex/em 488 nm/515 nm ...
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bioRxiv - Bioengineering 2024Quote: ... 100 μM non-essential amino acids (both Thermo Fisher Scientific, Waltham, MA), 0.13 mM L-ascorbic acid-2-phosphate and 100 nM dexamethasone (both Sigma Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... all secondary antibodies were purchased from Thermo Fisher Scientific and used at an 1:500 dilution for 2h at RT if not stated otherwise ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were halted by addition of 3X Loading Dye containing 250 mM DTT and resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris, Invitrogen). The samples were transferred to nitrocellulose membrane and blocked in 5% BSA ...
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bioRxiv - Biochemistry 2024Quote: ... that was developed using 100uL of TMB reagent (Thermo Fisher Scientific). Absorbance was read at 630nm ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products were purified from template plasmid and primers by two-step bead purification using SPRI beads (Cytiva) and quantified using Qubit 3.0 High Sensitivity dsDNA kit (Invitrogen). Indexed libraries across ligand and bin conditions were pooled in equimolar ratios and submitted to the NUSeq Core Facility for sequencing on a full flowcell of a NextSeq 500 (Illumina ...
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bioRxiv - Biochemistry 2024Quote: ... The reactions were halted by addition of 2X Loading Dye containing 250 mM DTT and resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris, Invitrogen). For FAM-labelled nucleic acids ...
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bioRxiv - Biochemistry 2024Quote: ... was prepared in serum-free media (Opti-MEM™; Gibco™, cat # 31985062). A ∼3:1 ratio of X-tremeGENE™ 9 DNA Transfection Reagent (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... viability of HPAFII cells was assessed using PrestoBlue reagent from Thermo Fisher (as per manufacturer instructions). Values were corrected to sera-only treated cells ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were incubated with DyLight 800 conjugated NeutrAvidin (Thermo Fisher Scientific, cat. no. 22853; 1:10,000) and visualised using an Odyssey CLx Imaging System (LI-COR Biosciences).