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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and CB (donkey anti rabbit, 1:200, A1042, Invitrogen). Nuclear counterstains were done with Hoechst (H1399 ...
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bioRxiv - Neuroscience 2024Quote: ... CR (donkey anti goat, 1:200, A11058, Invitrogen) and CB (donkey anti rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... GlutaMax (1:100, Gibco, 35050) and penicillin and streptomycin (1:100 ...
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bioRxiv - Pathology 2024Quote: ... 500 ng of RNA was subjected to reverse transcription reaction using the SuperScript III First-strand Synthesis System (Thermo Fisher Scientific) as instructed ...
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bioRxiv - Pathology 2024Quote: ... coupled online to an Easy-nLC 1200 (Thermo Fisher Scientific). The mobile phases contain 0.1% formic acid in 100% H2O (solvent A ...
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bioRxiv - Pathology 2024Quote: ... Lungs were finely chopped and digested in 0.5% collagenase type IV (Gibco 17104-019) at 37°C for 45 min ...
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bioRxiv - Pathology 2024Quote: Total protein concentrations were assessed from all dialyzer membrane extracts using the Pierce™ BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). The absorbance was taken at 562 nm on a microplate reader ...
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bioRxiv - Pathology 2024Quote: ... Membranes were exposed to chemiluminescence (ECL) reagent (TK274306, Thermo Scientific) for 1 min at room temperature ...
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bioRxiv - Pathology 2024Quote: For some selected animals brain and liver were aseptically removed and samples directly frozen at −80° C or kept in RNAlater solution (Ambion) at −20° C until processing ...
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bioRxiv - Pathology 2024Quote: ... anti- RFP (Invitrogen, R10367) at 4°C overnight ...
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bioRxiv - Pathology 2024Quote: Live sorted tdTom+ cells were centrifuged and resuspend in 0.04% ultrapure BSA (Invitrogen, 01266574) in PBS for optimal cell concentration ...
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bioRxiv - Pathology 2024Quote: ... A mouse anti-human CD11b activated labeled with FITC (clone CBRM1/5, eBioscience) and elastin (EnzChek Elastase Assay Kit, ThermoFisher) were used in association with a rat anti-mouse LY6G antibody for the evaluation of neutrophils activation in cytometry ...
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bioRxiv - Pathology 2024Quote: ... was dissolved in 11.2 mM 3-isobutyl-1methylxanthine (IBMX; Sigma-Aldrich, I5879 and Dulbecco’s Modified Eagle Medium (DMEM; Gibco 10564011) for external calibration ...
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bioRxiv - Pathology 2024Quote: Liquid chromatography with tandem mass spectrometry was then used to quantify cAMP using an orbitrap Fusion Lumos mass spectrometer interfaced to an Ultimate3000 HPLC system (Thermo Scientific, West Palm Beach, FL) and an Atlantis C18 column (2.1×150mm ...
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bioRxiv - Neuroscience 2024Quote: ... anti-PSD95 (ThermoFisher Scientific, MA1-045), anti-vGluT1 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with B27 (2%, Thermo Fisher, 17504044) and GlutaMAX (2 mM ...
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bioRxiv - Neuroscience 2024Quote: ... combined with 20 µL 0.4% Trypan Blue (Invitrogen T10282), and counted on a Countess II automated cell counter (Invitrogen AMQAX1000 ...
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bioRxiv - Neuroscience 2024Quote: ... and stained with eBioscience Fixable Viability Dye eFluor 780 (Thermo Fisher) and Brilliant Violet anti-human CD45 antibody (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... macrophages were stimulated with 100 ng/ml LPS or 10 µg/ml Poly I:C HMW (Invitrogen) for six hours ...
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bioRxiv - Neuroscience 2024Quote: ... chicken anti-GFP (1:500, A10262, Thermo Fisher Scientific Inc ...
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bioRxiv - Neuroscience 2024Quote: ... TNF alpha and IL-6 were quantified using the Procartaplex assays from Invitrogen. To assess functionality of KO in iPSC-derived myeloid cells ...
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bioRxiv - Neuroscience 2024Quote: ... Mass spectrometry using LC-MS/MS was performed using Ultimate UHPLC system coupled to an Exploris 480 mass spectrometer with a FAIMS Pro interface (Thermo Fisher Scientific) The FAIMS compensation voltages were −45 and −65 V ...
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bioRxiv - Neuroscience 2024Quote: ... and rabbit anti-MAP2 (PA5-17646, Invitrogen, Thermo Fisher Scientific) in a 1:2000 solution ...
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bioRxiv - Pathology 2024Quote: The nephrotoxic effects of DMQ were investigated in the RPTEC cell line using a multi-parametric high content screening platform (Thermo Scientific Cell omics ArrayScan®) at eight-time points over nine days as described previously (Vormann ...
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bioRxiv - Pathology 2024Quote: ... Trypsin-EDTA and Accutase (Thermo Fisher Scientific, Waltham, MA) were used for cell detachment of HASMC and HAEC ...
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bioRxiv - Pathology 2024Quote: ... The sections were stained with hematoxylin (Roth, T865.2) for 2 min and eosin (Thermo Fisher Scientific, 6766007) for 1 min ...
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bioRxiv - Pathology 2024Quote: ... Solvent A and solvent B were Optima grade from Fisher Scientific. Parallel Reaction Monitoring of protonated cAMP at m/z 330.0598 ([M+H]+ ...
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bioRxiv - Pathology 2024Quote: ... Finally mounted with ProLong Gold Antifade Reagent containing DAPI (Molecular Probes, P36935).
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bioRxiv - Pathology 2024Quote: ... 1 μg of mRNA was used for the synthesis of first-strand cDNA using the High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific, Waltham, MA). cDNA templates were then amplified in triplicates with SYBR Green PCR Master Mix using the QuantStudio3 real-time qPCR system (Applied Biosystems ...
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bioRxiv - Pathology 2024Quote: ... scrambled siRNA or FOXN3 siRNA (10 nM) was transfected into the cells using Lipofectamine RNAiMAX Reagent (13778150, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Donkey anti-Rabbit 568 (Abcam ab175470 or ThermoFisher A31573, 1:800–1:1,000), and Donkey anti-Chicken 488 (Jackson ImmunoResearch 703-545-155 ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 minutes and then mounted on glass microscope slides (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated 90 minutes in the dark at room temperature with the corresponding secondary antibodies (Alexa Fluor 647 1:1000, Abcam ab150171; Alexa Fluor 555 1:1000, Invitrogen A-21429) diluted in PBS with 1 % bovine serum albumin and 0.3 % Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 6.5% (v/v) fetal bovine serum (ThermoFisher Scientific #12483020) and 1X GlutaMAX (ThermoFisher Scientific #35050061) ...
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bioRxiv - Neuroscience 2024Quote: ... which consists of Opti-MEM media (ThermoFisher Scientific #31985088) supplemented with 6.5% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... the slides were incubated overnight at 4°C with primary antibodies (anti-GFAP 1:1000, Invitrogen #PA1-10004 ...
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bioRxiv - Neuroscience 2024Quote: ... After 4 times 5-minute washes in PBS with 0.5 % Tween® 20 (Fisher Scientific #9005-64-5), each slide was again dried around tissue slices and PAP pen was re-applied ...
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bioRxiv - Neuroscience 2024Quote: ... ProLong® Gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen #P36931) was added to each slide ...
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bioRxiv - Neuroscience 2024Quote: ... This equipment comprises an Ultimate 3000 liquid chromatography system coupled to a Q-Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Scientific). Five hundred nanograms of peptides of each sample were loaded onto a trapping cartridge in a mobile phase of 2% ACN ...
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bioRxiv - Pathology 2024Quote: ... the cells were incubated with rhodamine-phalloidin (1:400, R415, Thermo Fisher Scientific) for 60 min at room temperature ...
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bioRxiv - Pathology 2024Quote: Total RNA was extracted from treated HASMCs using TRIzol reagent (15596018, Invitrogen). Gene expression was quantified with an SYBR Green real time-PCR using the ViiA™ 7 system (4453535 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... selection with Geneticin™ (600 ng/μL; G418 sulfate; Cat.#10131027; Gibco) was performed for 1 week to enrich for H4 cells expressing the splicing acceptor – T2A self-cleaving peptide (SA-T2A)-linked Neomycin resistance cassette (NeoR/KanR) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293T cells were purchased from ATCC and cultured in high glucose DMEM with 2 mM GlutaMAX and pyruvate (Life Technologies catalog #10567022) supplemented with 10% heat-inactivated characterized fetal bovine serum (Hyclone ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Protein concentration was evaluated using a Pierce™ BCA Protein Assay Kit (Cat.#23225; Thermo Scientific) by diluting samples in 1% Triton X-100 lysis buffer.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Hoechst 33342 solution (Cat.#62249; ThermoFisher Scientific) was diluted in PBS to 5 µg/mL and added during the third wash ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Total Exosome RNA & Protein Isolation Kit (Invitrogen) (Catalog number ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were stored at 4°C in PBS with NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI) (Cat.#R37606; ThermoFisher Scientific) until imaging ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... ProLong Diamond Antifade Mountant with DAPI (Thermo Fisher Scientific) was used to stain nuclear DNA ...
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bioRxiv - Pathology 2024Quote: ... Protein concentration was determined by colorimetric detection method (Pierce BCA protein Assay, Thermo Fisher). Proteins were separated on 4-12% Bis-Tris gels (Invitrogen ...
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bioRxiv - Pathology 2024Quote: Total RNA was isolated using TRIzol reagent (Invitrogen Corp., Carlsbad, CA) from hip articular cartilage or mouse knee cartilage and was submitted to Admera Health (South Plainsfield ...