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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: Anthocyanins metabolic class was detected using a UPLC-ESI-MS/MS system (UPLC, ExionLC™ AD, https://sciex.com.cn; MS, Applied Biosystems 6500 Triple Quadrupole ...
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bioRxiv - Plant Biology 2024Quote: ... dsRNA was extracted using TRIzol™ reagent (Invitrogen, USA, Cat. No. 15596026) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... All solvents and acids had Optima grade for LC-MS (Thermo Fisher Scientific). Eluting peptides were on-line ionized by nano-electrospray (nESI ...
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bioRxiv - Plant Biology 2024Quote: ... Separation of peptides was performed by reverse-phase chromatography at a flow rate of 300 nL/min and a Thermo Scientific reverse-phase nano Easy-spray column (Thermo Scientific PepMap C18 ...
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bioRxiv - Plant Biology 2024Quote: ... cells were stained with Concanavalin A (ConA) – Alexa FluorTM 594 conjugates (ThermoFisher) for 10 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... and run for 1 h at 190 V in Novex Nupage (Invitrogen). Proteins were transferred onto nitrocellulose using a semidry transfer technique ...
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bioRxiv - Plant Biology 2024Quote: ... and hybridised with a whole-gene tobacco array according to the manufacturer’s user manual (Affymetrix, USA). Arrays were then washed and stained using the FS450_0001 protocol on a Fluidics station preloaded with stain cocktails 1 and 2 and an array holding buffer ...
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bioRxiv - Plant Biology 2024Quote: ... and quality were determined with a Qubit RNA Broad-Range Assay Kit (Invitrogen, Waltham, MA, USA) and a Qubit 4 benchtop fluorometer (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... goat anti-mouse Alexa Fluor Plus 488 and F(ab’)2-goat anti-mouse Alexa Fluor Plus 555 from Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... and subsequently treated with DNaseI (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The RNA was then quantified by measuring the absorbance (260 and 280 nm) using a NanoDropTM 1000 Spectrophotometer (Thermo Scientific), and cDNA was synthesized with Invitrogen SuperScript® II Reverse Transcriptase ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were loaded onto a pre-column (Thermo Scientific PepMap 100 C18 ...
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bioRxiv - Plant Biology 2024Quote: ... the supernatants were collected and evaporated in a Savant SpeedVac (Thermo-Fisher Scientific, Waltham, MA, USA) at maximum speed for two h ...
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bioRxiv - Plant Biology 2024Quote: ... The slurry was passed through a 10ml gravity flow-based column (Fisher scientific) and the column was washed stepwise with 1X column buffer supplemented with 1mM maltose ...
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bioRxiv - Plant Biology 2024Quote: ... The reaction was carried out using 2X SYBR green mix from Thermo Scientific (USA) and was run in the StepOne Real-Time PCR System from Applied Biosystems (USA). The qRT-PCR conditions used were as follows ...
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bioRxiv - Plant Biology 2024Quote: Open reading frames of selected candidate genes were amplified from cDNA prepared with Maxima Reverse Transcriptase (Thermo Fisher Scientific) according to manufactureres instruction using leaf RNA that had been extracted using the Spectrum Plant Total RNA Kit (Merck KGaA ...
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bioRxiv - Plant Biology 2024Quote: ... and a Qubit 4 benchtop fluorometer (Invitrogen, Waltham, MA, USA). RNA integrity was further assessed with a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... system and a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific Inc, Waltham, MA, USA). Separation of peptides was performed by reverse-phase chromatography at a flow rate of 300 nL/min and a Thermo Scientific reverse-phase nano Easy-spray column (Thermo Scientific PepMap C18 ...
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bioRxiv - Plant Biology 2024Quote: ... either an Orbitrap Velos Pro hybrid mass spectrometer or a Q Exactive HF mass spectrometer (both Thermo Fisher Scientific) were used ...
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bioRxiv - Plant Biology 2024Quote: ... LC-MS method programming and data acquisition was performed with the XCalibur 4.0 software (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2024Quote: ... second strand of cDNA and cRNA were synthesised using Affymetrix kits (Affymetrix, USA). Single-stranded cDNA was synthesised from purified cRNA ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were loaded on an Acclaim PepMap 100 pre-column (100 µm x 2 cm, C18, 5 µm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 µL/min for 3 min ...
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bioRxiv - Plant Biology 2024Quote: ... Analytical separation of peptides was accomplished on an Acclaim PepMap RSLC column (75 µm x 50 cm, C18, 2 µm, 100 Å; Thermo Fisher Scientific) at a flow rate of 300 nL/min ...
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bioRxiv - Plant Biology 2024Quote: ... Constructs were amplified with the Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific, Waltham, MA, USA) following manufacturer’s instructions and using primers listed in Suppl ...
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bioRxiv - Plant Biology 2024Quote: ... 0.5 µg RNA was reverse transcribed (Maxima Reverse Transcriptase; Thermo Fisher Scientific). The reaction product was diluted 1:10 in double-distilled water before qPCR ...
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bioRxiv - Plant Biology 2024Quote: All LC-MS/MS experiments were performed using a Dionex Ultimate 3000 RSLC nanoUPLC (Thermo Fisher Scientific Inc, Waltham, MA, USA) system and a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific Inc ...
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bioRxiv - Plant Biology 2024Quote: ... 1 to 3 µl of each sample were subjected to reverse phase LC for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Peptides were loaded on an Acclaim PepMap 100 pre-column (100 µm x 2 cm ...
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bioRxiv - Plant Biology 2024Quote: ... Eluting peptides were on-line ionized by nano-electrospray (nESI) using the Nanospray Flex Ion Source (Thermo Fisher Scientific) at 1.5 kV (liquid junction ...
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bioRxiv - Plant Biology 2024Quote: ... The reaction was carried out using 2X SYBR green mix from Thermo Scientific (USA) and was run in the StepOne Real-Time PCR System from Applied Biosystems (USA) ...
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bioRxiv - Plant Biology 2024Quote: ... The LC eluant was sprayed into the mass spectrometer by means of an Easy-Spray source (Thermo Fisher Scientific Inc.). All m/z values of eluting ions were measured in an Orbitrap mass analyzer ...
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bioRxiv - Plant Biology 2024Quote: All plasmids were generated using Gateway technology (Invitrogen, Life Technologies). pDONR vectors carrying the CDS of D14 and SMXL7 were recombined with pDEST plasmids to generate binary vectors by LR Clonase reactions ...
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bioRxiv - Plant Biology 2024Quote: ... Quantification of each compound was done by manual integration of peak area using Xcalibur 3.0 (Qual Browser, Thermo Scientific) and abundance was calculated by reference to the authentic standards ...
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bioRxiv - Plant Biology 2024Quote: ... 30 μL of the supernatant was then transferred to glass vials for injection onto a Vanquish Horizon UHPLC Binary Pump H coupled to a Q-Exactive mass spectrometer (Thermo Scientific). All reagents were of LC-MS quality ...
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bioRxiv - Plant Biology 2024Quote: ... 58 were spiked into each sample prior to loading onto a Fe3+-IMAC column (Propac IMAC-10 4×50 mm, Thermo Fisher Scientific). The enrichment was performed with Buffer A (0.07% TFA ...
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bioRxiv - Plant Biology 2024Quote: ... 9-day-old seedlings were transferred onto 1% plant agar MS medium plates containing different concentrations of zeocin (0 μM, 10 μM, 50 μM, 170 μM) (Gibco, 10072492). Seedling roots were cut with a razor blade and collected after overnight zeocin exposure ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 1 μg of RNA was treated with DNase (Thermo Fisher, EN0521) and reverse transcribed with Reverse Transcriptase (Thermo Fisher ...
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bioRxiv - Plant Biology 2024Quote: ... and reverse transcribed with Reverse Transcriptase (Thermo Fisher, EP0441). This template was then used to quantify relative mRNA abundance using the SensiMix SYBR Low-ROX kit (Bioline) ...
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bioRxiv - Plant Biology 2024Quote: ... TaqMan PCR assays were used following the manufacturer’s protocol (Applied Biosystems). Small nuclear RNA (snoR41Y ...
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bioRxiv - Plant Biology 2024Quote: ... 8 and 6 hours were then transferred to MS medium containing zeocin with Actinomycin D (Thermo Fisher, J60148.LB0) or zeocin with DMSO (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... Seedlings were transferred onto MS medium plates containing selected concentrations of zeocin (Gibco, 10072492) or no zeocin for control sample ...
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bioRxiv - Plant Biology 2024Quote: ... in a Fast 7500 Thermal Cycler instrument (Applied Biosystems). The expression was normalised using Tubulin and analysed through the comparative ΔΔCT method (Schmittgen and Livak 2008) ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were incubated with Click-iT reaction cocktail (Invitrogen C10269) mixed according to the manufacturer’s instructions with addition of Alexa Fluor 488 azide (Thermo Fisher ...
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bioRxiv - Plant Biology 2024Quote: ... containing DAPI diluted 1:1000 (Thermo Fisher, 62248).
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bioRxiv - Physiology 2024Quote: ... RNA was extracted form liver samples using standard Trizol procedure (Invitrogen). Brains were cryosectionned (Leica CM3050 ...
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bioRxiv - Plant Biology 2024Quote: ... and 2 µl was used as a template for qRT-PCR performed using Fast SYBR Green Mix (Applied Biosystems) in a Fast 7500 Thermal Cycler instrument (Applied Biosystems) ...
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bioRxiv - Physiology 2024Quote: ... each immediately mounted onto a single PEN Membrane Glass Slide (LCM0522, ThermoFisher) and allowed to dry for a few seconds ...
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bioRxiv - Plant Biology 2024Quote: ... LC-MS/MS analysis was performed on a Q Exactive HF Orbitrap (Thermo Fisher Scientific) coupled on-line to a Dionex Ultimate 3000 RSLCnano system ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was isolated by TRIzol reagent (Invitrogen, 15596018). Library construction and sequencing were carried out by BGI (Wuhan ...
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bioRxiv - Plant Biology 2024Quote: ... Mixture of Dynabeads with proteins A and G (1:1) (Invitrogen) was used to pull-down the protein/DNA complexes and DNA was purified using ChIP Clean-up and Concentrator kit (Zymo Research) ...
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bioRxiv - Plant Biology 2024Quote: ... fluorescence was observed under a confocal microscope (LSM880NLO, Zeiss, Germany) using 2’,7’-dichlorodihydro fluorescein diacetate (H2DCFDA, 10 InvM, Invitrogen, USA) as described by Yu et al ...