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Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and 0.5 μg was used for cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Waltham, MA). qPCR was performed with the SYBR Green PCR Master mix (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were lysed using 100uL of TRIzol Reagent (Invitrogen), agitated via pipetting and vortexing for 30 seconds ...
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bioRxiv - Bioengineering 2024Quote: ... RNA was treated with DNase using the DNase I kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... qPCR was performed with the SYBR Green PCR Master mix (Applied Biosystems, Waltham, MA) and custom-designed qRT-PCR primers (IDT ...
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bioRxiv - Bioengineering 2024Quote: ... the Neon™ Electroporation System 10-μL Kit (Invitrogen, Waltham, MA, USA) was used on the Neon Transfection System (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... using a PureLink® Genomic DNA Minikit (Invitrogen). The target amplicon DNA containing the desired CRISPR cut site was extracted using primers (Supplement Table 1) ...
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bioRxiv - Bioengineering 2024Quote: ... then 2.5:1 duRNA:Cas9 molar solution was made in Dulbecco’s phosphate buffered saline (DPBS; Gibco) to a final RNP concentration of 9.2 μM ...
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bioRxiv - Bioengineering 2024Quote: ... Flow cytometry analysis of enucleated or viable cells was performed using double-stranded DNA dyes (DRAQ5, 65-0880-96, Invitrogen and 7AAD, 559925, BD, respectively).
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bioRxiv - Bioengineering 2024Quote: ... cells were washed in DPBS twice before staining with NucBlue Live Cell Stain (Hoechst 33342, ThermoFisher) and CellMask Deep Red membrane stain (ThermoFisher ...
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bioRxiv - Bioengineering 2024Quote: ... was used on the Neon Transfection System (Invitrogen). Electroporation kit V and Electroporation kits for Primary T-Cell/HSPC were purchased from Lonza (Basel ...
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bioRxiv - Bioengineering 2024Quote: HEK293T cells were transfected in 24-well plates with Lipofectamine 2000 (Invitrogen) via reverse transfection following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were then treated with 0.5% Triton X-100 (Thermo Fisher) and 50 units/mL Benzonase (Merck ...
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bioRxiv - Bioengineering 2024Quote: ... Samples then had 1 μL Proteinase K and 1 μL RNase A added (both from Invitrogen) and were incubated for 10 min at 28 °C ...
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bioRxiv - Bioengineering 2024Quote: ... and CellMask Deep Red membrane stain (ThermoFisher) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The supernatant was collected into a fresh tube and mixed with 40% polyethylene glycol 8000 (Thermo Fisher) solution in a ratio of 4:1 by volume and stored overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... Concentrations of eluted DNA other than genomic DNAs were determined by Qubit (Qubit™ ssDNA Assay Kit, ThermoFisher, Cat. No. Q10212) from 3 independent measurements.
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bioRxiv - Bioengineering 2024Quote: ... The hard diameter of Au cores was determined by transmission electron microscopy (TEM; Talos L120C, ThermoFisher Scientific). Concentration was determined with 1 cm Beer–Lambert law absorbance at 520 nm (Nanodrop One ...
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bioRxiv - Bioengineering 2024Quote: ... The target DNA was purified by PureLink® PCR cleanup (Invitrogen), then the target exon was extracted with B2M exon primers with attached Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Bioengineering 2024Quote: ... and collagen were determined via a modified Quant-iT PicoGreen dsDNA assay kit (Invitrogen), 1,9-dimethylmethylene blue (DMMB ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were quantitated using the Qubit BR dsDNA kit (ThermoFisher, Cat. No. Q32850). A total of 500 ng of genomic DNA was used for bisulfite conversion using the Zymo EZ DNA Methylation kit (Zymo Research ...
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bioRxiv - Bioengineering 2024Quote: ... were cultured in Gibco Roswell Park Memorial Institute (RPMI)-1640 media + 10% fetal bovine serum (FBS) + 1% Penicillin/Streptomycin (ThermoFisher) at 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... 5 mM 3,4-Dihydroxybenzoic acid (Fisher Scientific, Cat. No. AC114891000), 50 nM protocatechuate dioxygenase (Millipore Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... Target DNA was purified with a PureLink® PCR cleanup kit (Invitrogen). 500 ng of target DNA was then added to PCR strip tubes ...
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bioRxiv - Bioengineering 2024Quote: ... samples were washed with UltraPure Water (UPW; Invitrogen, USA) twice to generate the product designated “AuRNA” stage ...
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bioRxiv - Bioengineering 2024Quote: ... then SDS-PAGE was performed using NuPAGE™ 4-12% Bis-Tris Mini Protein Gels (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Penicillin/streptomycin (Gibco, 15140) 1% (v/v) ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were screened and imaged on a Talos Glacios 200kV microscope (ThermoFisher) equipped with a Selectris energy filter and Falcon 4i direct electron detector (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... HER2 with a C-terminal tail truncation (λ−1030–1255) followed by maltose binding protein (MBP) and twin-strep tags was cloned into pFastBac1 with a CMV promoter (Thermo Fisher Scientific). Point mutations were introduced in the HER2 kinase domain ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mM) and diluted in DMSO transferred to wells of PCR Plates (Micro Amp Fast 96-well Reaction Plates, Applied Biosystems) to ensure matching final DMSO concentrations.
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bioRxiv - Biochemistry 2024Quote: ... 10,340 movies were collected using a Talos Arctica microscope (Thermofisher) operating at 200kV equipped with a K3 direct electron detector (Gatan) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.12 x 106 COS-7 cells were seeded into each well of a 6 well plate and transfected with 1 μg total DNA using Lipofectamine p3000 (ThermoFisher Scientific). Cells were rinsed in PBS 5 hours post transfection ...
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bioRxiv - Biochemistry 2024Quote: ... at 1 mg/mL final concentration and propidium iodide (PI, Invitrogen) at 1 μg/mL final concentration and incubated at room temperature in the dark for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... LB + 1.5% agarose slabs (∼0.2 mm thick) were prepared on a 75 x 25 mm microscopy slide (Fisher Scientific). Agarose slabs contained staining dyes at the same concentration and optionally were supplemented with 0.2% L-arabinose ...
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bioRxiv - Biochemistry 2024Quote: ... followed by washing and addition of 100 µL/well of goat anti-human IgG-HRP conjugate (Invitrogen, 62-8420) at 1:500 dilution ...
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bioRxiv - Biochemistry 2024Quote: ... All protein samples were buffer exchanged into 50 or 200 mM ammonium acetate (Fisher Scientific 10365260) as indicated using Zeba Micro Spin columns (#89877,75µL ...
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bioRxiv - Biochemistry 2024Quote: ... Transamination products were analyzed using Vanquish UHPLC system coupled with the Q Exactive Quadrupole-Orbitrap MS (Thermo Fisher Scientific, Waltham, MA) with negative ionization using settings as previously described117 ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 µl of the PCR product was used in a Big Dye v3.1 (Applied Biosystems) sequencing reaction ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant baculovirus was prepared using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific). Spodoptera frugiperda Sf9 insect cells (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... and Phe (164.0692 - 164.0742) was analyzed using Vanquish UHPLC system coupled with the Q Exactive quadrupole-Orbitrap MS (Thermo Fisher Scientific, Waltham, MA) with negative ionization using settings as previously described117 ...
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bioRxiv - Biochemistry 2024Quote: ... using commercially synthesized fluorescein-labeled oligonucleotides (Thermo Fisher, Waltham, MA, USA). Because TREX1 starts catalyzing the degradation of DNA substrate once the conducive buffer condition is provided ...
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bioRxiv - Biochemistry 2024Quote: ... Ncap proteins were immobilised in microtitre plates (Immulon 4HBX; Thermo Scientific, 6405) at 4 °C overnight at 2 μg/mL (50 µL per well ...
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bioRxiv - Biochemistry 2024Quote: ... the absorbance determined at 280 nm (NanoDrop™ One, Thermo Scientific), and concentrations calculated using extinction coefficients taken from ProtParam.
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bioRxiv - Biochemistry 2024Quote: Recombinant GCase N370S mutant was produced by using 293T Freestyle cells (Thermo Fisher Cat. No. R79007) grown in suspension in 293 Freestyle medium (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... sugars were separated using a CARBOPAC PA-1 anion exchange column with a PA-1 guard using a Dionex ICS-6000 (ThermoFisher) and detected using pulsed amperometric detection (PAD) ...
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bioRxiv - Biochemistry 2024Quote: ... Microscope calibrations and automatic data acquisition were performed with EPU software (Thermo Fisher Scientific) at a nominal magnification of ×130k ...
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bioRxiv - Biochemistry 2024Quote: ... phosphate buffer solution (PBS, Thermo Scientific) solution was layered at the bottom of SW40Ti ultra-clear tube (Beckman Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... 1:1000 dilution mouse anti-OxPhoS cocktail antibody (Invitrogen, 45-8099), 1:1000 dilution rabbit anti-HSL antibody (Novus biologicals ...
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bioRxiv - Biochemistry 2024Quote: ... at 0.8 mg/ml were applied to glow discharged Quantifoil® 300 mesh R 0.6/1 grids and flash frozen in liquid ethane using FEI Vitrobot MAG IV (Thermo Fisher Scientific). For AtRuvBL1-AtRuvBL2a complex ...
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bioRxiv - Biochemistry 2024Quote: ... then transfected using 1 µg of pCMV_GBA_N370S_His and 3µg of PEI per 106 cells in OPTIMEM (Life Technologies). 12 hours after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... Blots were then incubated with Odyssey blocking buffer for 30 min at room temperature and incubated with primary antibodies (1:1000 dilution rabbit anti-PTER antibody (Invitrogen, PA5-20750), 1:5000 dilution rabbit anti-β-actin antibody (Abcam ...