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Citations for Thermo Fisher :
3651 - 3700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and 1% of penicillin-streptomycin (10,000 U/ml, Thermo Fisher Scientific) at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... and treated with purified viral particles resuspended in 0.8 mL DMEM containing 8 µg/mL polybrene (Thermo Fisher Scientific). The cells were spinfected by centrifuging the six-well plates at 1050 x g for 90 min at 30 °C and the returned to the 37 °C incubator for another 48 h ...
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bioRxiv - Bioengineering 2024Quote: ... and the concentration was evaluated with a NanoDrop OneC (ThermoFisher Scientific). A High Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... at concentrations ranging from 1.6-16 mM for 90 minutes before being detached from the surface by treatment with 0.25 % trypsin-EDTA (Thermo Fisher Scientific). The detached cells were then centrifuged at 350 x g for 5 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... was performed using custom primers for 16S rRNA gene (V1-V3 regions)26 (27F:AGAGTTTGATCMTGGCTCAG; 518R:GTATTACCGCGGCTGCTGG) (Thermo-Fisher Scientific Waltham, MA). Master mix (Thermo-Fisher Scientific Waltham ...
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bioRxiv - Bioengineering 2024Quote: ... and Qubit® (Thermo Fisher Scientific, Waltham, WA). Final libraries were sequenced on Illumina® MiSeq™ with a V3 reagent kit (600 cycles ...
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bioRxiv - Bioengineering 2024Quote: ... was performed according to manufacturer’s instructions and gDNA content was quantified using a nanodrop (Thermo-Fisher Scientific, Waltham, MA). 300 ng of gDNA/sample were processed for sequencing analysis ...
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bioRxiv - Bioengineering 2024Quote: ... Master mix (Thermo-Fisher Scientific Waltham, MA) components for a final volume of 50 µL were ...
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bioRxiv - Bioengineering 2024Quote: ... Grids were screened at the Stanford cEMc on a 200 kV Glacios microscope (Thermo Fisher) equipped with a K3 camera (Gatan) ...
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bioRxiv - Bioengineering 2024Quote: ... which were blotted for 2 s at 4°C and 100% humidity with an offset of −15 and plunged frozen into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). Grids were screened at the Stanford cEMc on a 200 kV Glacios microscope (Thermo Fisher ...
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bioRxiv - Bioengineering 2024Quote: ... rat anti-Ter119-Super Bright 436 (1:100, ThermoFisher, 62-5921-82), and goat anti-CD45 (1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... and brains were collected in ice-cold Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher Scientific) mixed with penicillin/streptomycin (100 U/mL ...
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bioRxiv - Biochemistry 2024Quote: ... a Vanquish binary pump system (Thermo Scientific) was used with a Waters Acquity Premier CSH Phenyl-Hexyl column (100 mm × 2.1 mm ...
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bioRxiv - Plant Biology 2024Quote: ... and its purity was evaluated by spectrophotometry using a Nanodrop 2000 (Thermo Fisher Scientific). PacBio libraries were prepared from both species using the SMRTbell prep kit v3.0 ...
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bioRxiv - Plant Biology 2024Quote: ... We conducted quantitative reverse transcription PCR (qRT-PCR) with three technical replicates for each sample using SYBR Green super mix dye (Thermo Fisher Scientific™) and the CFX Opus 384 and Bio-Rad CFX Maestro platforms ...
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bioRxiv - Plant Biology 2024Quote: The yeast two-hybrid assay was performed according to previous publications using the ProQuest Two-Hybrid System (Invitrogen) following the manufacturer’s instructions52 ...
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bioRxiv - Plant Biology 2024Quote: ... Magnetic beads with IP products were precipitated magnetically using a DynaMagnetic rack (Thermo Fisher) and then washed five times with 1 mL of co-IP buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Quality and yield of the DNA samples were checked using a Qubit 4 (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Plant Biology 2024Quote: ... The reaction was carried out using 2X SYBR green mix from Thermo Scientific (USA) and was run in the StepOne Real-Time PCR System from Applied Biosystems (USA). The qRT-PCR conditions used were as follows ...
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bioRxiv - Plant Biology 2024Quote: TMT (11-plex) reagents (ThermoFisher, Cat#A34808) were reconstituted in anhydrous acetonitrile (0.01mg/uL) ...
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bioRxiv - Plant Biology 2024Quote: ... The protein concentration was assessed using the Qubit protein assay (ThermoFisher Scientific) and all samples were adjusted to the same concentration using nuclear lysis buffer ...
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bioRxiv - Plant Biology 2024Quote: ... or anti-pTAC3 antibody (PhytoAB, San Jose, CA, USA; catalog number PHY0391A) and with 60 μL Protein A Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were quantified using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific). We pooled 2–3 RNA samples from the same tissue for library preparation to make pools of five different L ...
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bioRxiv - Plant Biology 2024Quote: ... The qPCR was performed using the Takyon ROX SYBR MasterMix blue dTTP kit and the QuantStudio5 (Applied Biosystems). The primers used for the ChIP-qPCR analysis are listed in Supplementary Information Table S1.
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bioRxiv - Plant Biology 2024Quote: ... was prebound to the equilibrated IgG coated Dynabeads (Thermo Fisher) for three hours with gentle rotation at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... The purity of RNA was evaluated utilizing a NanoDrop spectrophotometer (Thermo Scientific, DE, USA), while its integrity was verified through an Agilent 2100 (Agilent Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant plasmid and the empty pBI121 vector underwent digestion using the restriction enzymes XbaI and SacI (Thermo Scientific TM). The purified digested insert and vector ligated using T4 DNA ligase at a 3:1 ratio ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was carried out using random hexamer primers with RevertAid H Minus (Thermo Fisher Scientific™) in accordance with the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... cDNAs and qPCR primers were combined with 2X SYBR master mix from Thermo Fisher for qPCR ...
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bioRxiv - Plant Biology 2024Quote: ... and transferred to a 0.1 µm nitrocellulose membrane (Amersham Biosciences Protran) by semi-dry electroblotting for 1 h under constant current (60 mA) (Thermo Fisher Scientific) in filter paper soaked in transfer buffer (assembly order from the cathode to the anode ...
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bioRxiv - Plant Biology 2024Quote: ... The TraceFinder software (Thermo Scientific, Waltham, MA, USA) was used for data processing ...
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bioRxiv - Plant Biology 2024Quote: ... High-fidelity Taq DNA polymerase (Phusion Taq, Thermo Fisher Scientific, Massachusetts, USA) was employed in this process ...
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bioRxiv - Plant Biology 2024Quote: ... transferred to new boxes containing ½ MS medium supplemented with 10 μM EdU (Invitrogen) and cultured for 2 h to achieve a recovery time of 24 h ...
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bioRxiv - Plant Biology 2024Quote: ... 1 μl was injected into an Orbitrap Exploris 120 mass spectrometer coupled to a Vanquish UHPLC system (Thermo Fisher Scientific, Waltham, MA, USA). Reverse-phase ultraperformance liquid chromatography was performed using an Acquity PREMIER BEH C18 UPLC column (2.1 x 150 mm ...
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bioRxiv - Plant Biology 2024Quote: ... This fragment was cloned into the pDONR221 vector using Gateway BP recombination (Thermo Fisher Scientific) to generate pENTR-5’gSMR1 ...
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bioRxiv - Plant Biology 2024Quote: Flavonoid metabolic class was analyzed using a UPLC-ESI-MS/MS system (UPLC, SHIMADZU Nexera X2, https://www.shimadzu.com.cn/; MS, Applied Biosystems 4500 Q TRAP ...
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bioRxiv - Plant Biology 2024Quote: Cell death in plant leaves was quantified as previously described (Sang et al., 2020; Yu et al., 2020) by measuring the electrolyte leakage using a conductivity meter (ThermoFisher, USA) or observing the autofluorescence using the BioRad Gel Imager (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... against a 6.25 to 200 nmol D-(+)-galacturonic acid monohydrate (AAJ6628214, Thermo Fisher Scientific) standard curve.
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bioRxiv - Plant Biology 2024Quote: ... Trypsin-digested proteins were desalted and analyzed by LC-MS/ MS using an Eksigent nlc425 device coupled to a Q Exactive mass spectrometer (ThermoFisher Scientific), with a glass needle (non-coated capillary silica tips ...
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bioRxiv - Plant Biology 2024Quote: ... consisting of 10 μl of SYBR Green mix (PowerUp™ SYBR™ Green Master Mix, Applied Biosystems™), 1.6 μl of Rep (AC1 ...
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bioRxiv - Plant Biology 2024Quote: ... using a 7500 Fast Real-Time PCR System and the associated 7500/7500 software (Applied Biosystems). Relative expression levels were calculated according to the 2-ΔΔCt method 55 ...
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bioRxiv - Plant Biology 2024Quote: ... The cell pellet was overlaid with 143 µL of 70% nitric acid (trace metal grade, A467-500, Fisher Scientific) and incubated at 65°C for 16 h before dilution with 9.5 mL Milli-Q H2O to a final nitric acid concentration of 2% (v/v ...
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bioRxiv - Plant Biology 2024Quote: ... the 5’ genomic region of pENTR-5’gSMR1 was cloned upstream of the GUS gene into the pMpGWB104 binary vector 53 using Gateway LR recombination (Thermo Fisher Scientific). The 3’ genomic region of pGEMT-3’gSMR1 was cloned into the resulting vector as AscI/SacI ...
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bioRxiv - Plant Biology 2024Quote: ... bound on anti-FLAG dyna beads (Invitrogen). Eluted DNA was purified using a Minelute PCR purification kit (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... quantified by HR ICP-MS (Thermo Scientific, Element 2TM). For each sample ...
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bioRxiv - Plant Biology 2024Quote: ... The triple 3A mutant fragment was cloned into pENTR-D-TOPO (Thermo Fisher Scientific, MA, USA). The 3A mutant was used as a template to generate the 5A mutant ...
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bioRxiv - Plant Biology 2024Quote: ... Their PCR products were cloned into pJET1.2 blunt plasmids (Thermo Fisher Scientific, Massachusetts, USA) and sequenced.
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bioRxiv - Plant Biology 2024Quote: ... The ripE1 fragment in pDONR207 was used as template to generate ripE1 phosphosite mutants by site-directed mutagenesis using the QuickChange Lightning Site-Directed Mutagenesis Kit (Life technologies, USA) following the manufacturer’s instructions and the primers indicated in Table S2 ...
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bioRxiv - Plant Biology 2024Quote: Four days-old seedlings grown in vertical plates under our standard conditions were transferred to new plates containing fresh ½ MS medium (mock samples) or ½ MS medium containing 20 μg/ml zeocin (Invitrogen) or 3 μM methyl viologen dichloride hydrate (MV ...
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bioRxiv - Plant Biology 2024Quote: The cDNA samples were synthesized from 2 µg of RNA using a High-capacity cDNA synthesis kit (Applied Biosystems). RT-qPCR was performed using the ABI SYBR green kit as per manufacturer’s recommendation ...