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Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... was injected and analysed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) with a tandem UltiMate 3000 RSLCnano system in-line connected to an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific). Raw files were processed with the MaxQuant software (1.6.9.0 ...
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bioRxiv - Plant Biology 2024Quote: ... HRE2 and RAP2.3 in pENTR-D/TOPO (kindly provided by Prof. Margret Sauter, Christian-Albrechts University, Kiel, Germany) recombined into pDEST22 (Thermo Fisher Scientific, Waltham, USA), possessing the GAL4 activation domain ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted from the cultivars using TRIzol (Invitrogen, CA, USA), eukaryotic mRNA was enriched with magnetic beads with Oligo(dT) ...
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bioRxiv - Plant Biology 2024Quote: All inserts were PCR amplified using Phusion High Fidelity Polymerase (Thermo Fisher Scientific, Waltham, MA, USA), primers containing attB sites at their 5′ ends (Supplemental Table S4) ...
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bioRxiv - Plant Biology 2024Quote: ... 11 μl of RnaseCocktail (ThermoFisher, ref AM2288) were added and the lysate was incubated in a rotator at 37°C for 20 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... 110 μl of ProteinaseK (ThermoFisher, ref QS0511) were then added and samples were incubated in a rotator at 37°C for a further 20 minutes ...
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bioRxiv - Immunology 2024Quote: Whole cell extracts were lysed in 1X NuPage LDS Sample Buffer (ThermoFisher), and Protein was loaded in each lane of a 4 – 12% Bolt Bis-Tris Plus gel (ThermoFisher) ...
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bioRxiv - Plant Biology 2024Quote: RT-qPCR was carried out in 96-well plates with three technical replicates per sample using a StepOnePlusTM Real-Time PCR System (Applied Biosystems, USA). RT-qPCR design ...
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bioRxiv - Plant Biology 2024Quote: ... and checked by agarose gel (0.8%) electrophoresis stained with SYBR Safe (Thermo Fisher Scientific). The DNA concentration was determinate using the Qubit 4 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... jasmonic acid (JA) and abscisic acid (ABA) by ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS, Q-Exactive, ThermoFisher Scientific)26.
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bioRxiv - Plant Biology 2024Quote: ... Chromatographic separation was achieved on a Hypersil GOLD C18 Selectivity HPLC Column (150 mm x 4.6 mm; 3 μm; Thermo Fisher Scientific), utilizing a mobile phase comprising water (A ...
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bioRxiv - Immunology 2024Quote: ... and Protein was loaded in each lane of a 4 – 12% Bolt Bis-Tris Plus gel (ThermoFisher), and was then electrophoretically separated ...
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bioRxiv - Plant Biology 2024Quote: ... The master mix containing 1lll×lllABsoluteTM QPCR SYBR® Green Mix (Thermo Scientific, Epsom, UK), 100 nM of each forward and reverse primer (Supplementary Table S4 ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized using the SuperScriptTM III First-Strand Synthesis System (Invitrogen) with random hexamers and 8 µl of RNA.
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bioRxiv - Plant Biology 2024Quote: ... RNA quantity and quality was assessed by NanoDropTM One/OneC (Thermo Fisher Scientific). cDNA was synthesized using the SuperScriptTM III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... 500ng of total RNA was reverse transcribed into Complimentary DNA (cDNA) in a 10μl reaction with the SuperScript™ IV First-Strand Synthesis System (ThermoFisher, no. K1622) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... bound antibodies were detected using a goat anti-rabbit secondary antibody coupled to a fluorescent molecule (Alexa Fluor 680, Invitrogen) diluted 1:10,000 using the “Odyssey Infrared Imager” at 680 nm (Licor ...
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bioRxiv - Plant Biology 2024Quote: ... and PCR products were visualised on 2% agarose gel stained with SYBR safe (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2024Quote: ... The remaining DNA was subjected to anti-GFP immunoprecipitation using Pierce Protein G Magnetic Beads (ThermoFisher, no. 88847) coated with monoclonal anti-GFP antibody (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... 0.22 mM Coomassie blue) and loaded onto a Bolt 8% Bis-tris protein gel (Thermo Scientific). The gel was run at 80 V for 15 min and then 160 V for 1 h until proper separation of bands were obtained ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were separated on an Acclaim (Thermo Scientific) column using a Dionex Ultimate 3000 UHPLC system ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentration was quantified using the “Protein Quantification BCA Assay” kit (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2024Quote: ... DNA was visualized by Glyco-Blue (ThermoFisher, no. AM9515) and precipitated in 70% ethanol ...
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bioRxiv - Plant Biology 2024Quote: ... was purchased from TCI America (via Fisher Scientific). All other chemicals ...
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bioRxiv - Plant Biology 2024Quote: ... The DNA concentration was determinate using the Qubit 4 (Thermo Fisher Scientific), and all samples were adjusted to 25 ng/µL for further studies.
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bioRxiv - Plant Biology 2024Quote: The degradation and contamination of RNA were monitored on 1% agarose gels and the quality and integrity of RNA were assessed using a NanoDrop spectrophotometer (840-317400, Thermo Scientific, DE, USA). A total amount of 1.5 μg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were diluted in nuclease free water and used for qRT-PCR reactions with the SYBR® Green PCR master mix (Applied Biosystems, www.appliedbiosystems.com) using an Applied Biosystems 7900HT Instrument ...
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bioRxiv - Plant Biology 2024Quote: ... and with Sypro Orange dye (Invitrogen) at a 2× final concentration ...
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bioRxiv - Plant Biology 2024Quote: ... A horseradish peroxidase-conjugated goat anti-rabbit IgG antibody from Life Technologies was employed as the secondary antibody at a dilution of 1:10,000 to detect the primary antibodies.
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bioRxiv - Plant Biology 2024Quote: ... Blots were developed with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific 34075) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... 5 mM EDTA, 5 mM DTT, 10 mM β-mercaptoethanol, 1% SDS, 1 mM PMSF, and 1X protease inhibitors from Thermo Fisher), and the plant debris was removed by centrifugation ...
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bioRxiv - Plant Biology 2024Quote: ... DNA was quantified using the Qubit DNA BR Assay Kit and a Qubit fluorimeter (Thermo Fisher Scientific) and its purity was evaluated by spectrophotometry using a Nanodrop 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: High pH reverse-phase chromatography was performed on the Vanquish Flex system (ThermoFisher) equipped with a 4.6×150-mm Gemini 5μm C18 column for TMT-labeled peptides ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 50 injections were measured on an Orbitrap Eclipse equipped with an Easy LC 1200 UPLC liquid chromatography system (Thermo Fisher). Peptides were first trapped in a trapping column (Acclaim PepMap 100 C18 HPLC ...
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bioRxiv - Plant Biology 2024Quote: ... Protein bands were visualized using chemiluminescence reagents (SuperSignal West Femto Maximum Sensitivity Substrate, Thermo Scientific) and a ChemiDoc Imaging System (Bio-Rad).
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... Genomic DNA and single-stranded RNA were removed from the dsRNA preparations by digestion for 45 min at 37°C with 1 µL DNase I Ambion TM (2 U/µl) (Thermo Fisher, Waltham, MA, USA) and 1µL RNase T1 (1000 U/µl ...
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bioRxiv - Plant Biology 2024Quote: ... 20 K MWCO (Thermo Fisher Scientific). Proteins were concentrated using Pierce™ Protein Concentrators PES ...
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bioRxiv - Plant Biology 2024Quote: ... PCR products were quantified with a Qubit®2.0 fluorometer (Invitrogen) and new PCRs performed for samples with concentration lower than 2.5 ng.µL-1 ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was carried out using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™, Foster City, CA, United States) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... BL21 Star™ (DE3) One Shot® (Invitrogen, Thermo Fisher Scientific) cells were transformed with the respective vectors and the transformed cells were used to inoculate 200 mL LB medium containing 50 mg/ml kanamycin and 0.025 % glucose ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... they were ligated in 20 µL reactions with the enzyme T4 DNA ligase (Thermo Fisher, Waltham, MA, USA) to get the pT777T-FcVDS ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... containing 0.4 µL of 1:1000 SYBR™ Green I Nucleic Acid Gel Stain (Thermo Fisher, Waltham, MA, USA). Primers FcRPB2-q-F and FcRPB2-q-R were used to amplify the F ...
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Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... Bacterial cells were concentrated by centrifugation at 4400 x g for 5 min) and used for total RNA extraction with TRIzol® (Thermo Fisher, Waltham, MA, USA) using ethanol washes at 65% ethanol instead of 75% to enrich the dsRNAs ...
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bioRxiv - Plant Biology 2024Quote: ... protein concentrations of fractions containing FtsZ1-2 and FtsZ2-1 were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... in each condition total RNA was extracted from a pool of 8 leaves and extracted using TRIzol reagent (Invitrogen). Three independent biological replicates were analysed ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was performed using an oligo-dT20 for a primer and Superscript II RNaseH-reverse transcriptase (Invitrogen). PCR was performed on a Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System with SYBR Green PCR MasterMix (Eurogentec) ...
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bioRxiv - Plant Biology 2024Quote: ... and cDNA was synthesised with M-MLV reverse transcriptase (Invitrogen), according to manufacturers’ instructions ...
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bioRxiv - Plant Biology 2024Quote: ... These PCR products were introduced into pDONR221 by BP reactions to produce four sGFP-tubulin entry vectors (Thermo Fisher, MA, USA). We performed the LR reaction of the pGWB501 destination vector with sGFP-tubulin entry vectors carrying pTUA1:sGFP-TUA1 ...
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bioRxiv - Plant Biology 2024Quote: ... All the PCR fragments were cloned in the pDONR207 entry vector (Invitrogen®), except for the PCR fragments to make the proLecRK-I.9::LecRK-I.9::tagRFP and the proLecRK-I.9::LecRK-I.9Δkinase::tagRFP constructs which were inserted between the EcoRI and BamHI sites of the Gateway® tagRFP-AS-N entry vector (Evrogen ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were developed with chemiluminescent substrate (Thermo Scientific) and exposed to film ...