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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Data analysis and chromatograms were implemented with the device-specific software Xcalibur (Thermo Scientific).
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bioRxiv - Physiology 2024Quote: Single cell suspensions were permeabilized for intracellular staining using FoxP3 fix/perm buffer (ThermoFisher) at 4 °C for 20 mins ...
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bioRxiv - Plant Biology 2024Quote: 10 µg of plasmid DNA linearized using SpeI was transformed into electrocompetent PichiaPink™ strain 4 (ThermoFisher; P. pastoris genotype ade2, pep4, prb1) targeted to the TRP2 locus ...
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bioRxiv - Physiology 2024Quote: ... GFP (1/1000, A11122, Invitrogen), GAPDH (1/1000 ...
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bioRxiv - Physiology 2024Quote: ... maintained at 8°C and coupled to an Orbitrap ELITE™ Hybrid Ion Trap-Orbitrap Mass Spectrometer (ThermoFisher Scientific). 5 µL of extract were injected into an Agilent Poroshell 120 EC-C18 column (2.1 x 100 mm ...
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bioRxiv - Plant Biology 2024Quote: ... transferred to a PVDF membrane and detected using Ponceau S staining or No-Stain™ Protein Labeling Reagent (ThermoFisher Scientific™, #A44449). Membrane blocking in 5% (w/v ...
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bioRxiv - Physiology 2024Quote: ... Lungs were filled with 600 µl RNAlater (Thermo Scientific, Darmstadt, Germany) dissected ...
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bioRxiv - Plant Biology 2024Quote: ... Screening of mutants followed the strategy previously described (35) and included pre-screening of potentially mutated DNA sequences by PCR amplification via DreamTaq (ThermoFisher Scientific Inc., Germany) and high-resolution melting curve analysis (HRM ...
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bioRxiv - Physiology 2024Quote: ... The SVF pellet was resuspended in 1 mL of Red Blood Cell ACK Lysis Buffer (Gibco) and incubated at room temperature for 3-5 min to lyse red blood cells ...
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bioRxiv - Physiology 2024Quote: ... Reverse transcription of the isolated RNA into cDNA was performed using the Maxima First Strand cDNA Synthesis Kit (Thermo Scientific, Darmstadt, Germany) according to the instructions of the manufacturer ...
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bioRxiv - Plant Biology 2024Quote: ... with EcoRI and NotI cloning sites was ordered from BioCat GmbH (www.biocat.com) and transformed into Escherichia coli BL21 Star (DE3) cells (ThermoFisher Scientific) by heat shock ...
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bioRxiv - Plant Biology 2024Quote: ... using the Pierce™ Bradford Plus Protein Assay Kit (ThermoFisher Scientific™, #23236). Ten micrograms of total protein were incubated at 96°C for 10 min in Laemmli Buffer (1:1 ...
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bioRxiv - Physiology 2024Quote: ... and subjected to digestion in 4 mL high glucose DMEM (Gibco) containing 1 mg/mL collagenase type II (cat# C6885 ...
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bioRxiv - Plant Biology 2024Quote: Genomic DNA for short-read sequencing was extracted from 5-week-old leaves using the MagJET™ Plant Genomic DNA Kit (Thermo Scientific, Cat. No. K2761). DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs Cat ...
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bioRxiv - Plant Biology 2024Quote: ... Extracts were treated with 1% (v/v) Halt™ Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific™, #78440) and quantified at 595 nm on the SPARK® multimode microplate reader (TECAN ...
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bioRxiv - Plant Biology 2024Quote: ... tricornutum strain UTEX 646 were PCR-amplified from cDNA using Phusion High-Fidelity DNA Polymerase (Thermo Scientific, Carlsbad, USA) and subcloned into the pGEM-T® Easy Vector (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... The pellet was washed with 75% ethanol and gently resuspended in sterile water and treated with RNAse A (Thermo Scientific™ EN0531) and Ribonuclease T1 (Thermo Scientific™ EN0541 ...
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bioRxiv - Plant Biology 2024Quote: ... Detection by chemiluminescence with Immun-Star AP Substrate (ThermoFisher Scientific, #1705018) was visualized using a Fusion FX Imaging system (Vilber ...
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bioRxiv - Physiology 2024Quote: ... isolated total RNA was subjected to reverse transcription using a high-capacity cDNA kit (Applied Biosystems, USA), following the manufactureŕs instructions ...
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bioRxiv - Plant Biology 2024Quote: ... and Ribonuclease T1 (Thermo Scientific™ EN0541) for 30 minutes at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... as well as upper and lower coleoptiles were microdissected (total area for each captured organ is over 0.3 mm2) and RNA extraction was performed using the ArcturusTM PicoPureTM RNA Isolation Kit (Thermo Fisher Scientific, Waltham, MA). The extracted RNA was amplified using ArcturusTM RiboAmpTM HS PLUS RNA Amplification Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: Exponentially growing cells were transformed using the GeneArt Max Efficiency Reagent (Thermo Fisher, Waltham, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA samples were treated with DNase using the TURBO DNA-free kit (Applied Biosystems). The RNA was used as a template for first-strand cDNA synthesis using SuperScript II reverse transcriptase (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was quantified using the Qubit HS-Sensitivity RNA kit and normalized for 1ng total RNA sample input per reaction (Thermo Fisher, Fremont, CA).
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bioRxiv - Plant Biology 2024Quote: ... The RNA was used as a template for first-strand cDNA synthesis using SuperScript II reverse transcriptase (Invitrogen) following the protocol of the manufacturer and using an Applied Biosystems GeneAmp PCR System 9700.
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA synthesis was carried out with SuperScript III reverse transcriptase (Invitrogen) following the manufacturer’s protocol in a total volume of 50 μl ...
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bioRxiv - Plant Biology 2024Quote: ... the slide was washed 3 times for 5 min with PBS followed by the addition of secondary antibodies to the PBS-T (Alexa Fluor 488 nm, anti-rabbit, 1/500 dilution; Alexa Fluor 568 nm, anti-mouse, 1/500 dilution; Invitrogen, Waltham, MA, USA) and incubated at room temperature for 1 h in the dark ...
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bioRxiv - Plant Biology 2024Quote: ... Prestained molecular weight standards were from Thermo Scientific (Spectra Multicolor High Range Protein Ladder #26625) ...
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bioRxiv - Plant Biology 2024Quote: ... The extracted RNA was amplified using ArcturusTM RiboAmpTM HS PLUS RNA Amplification Kit (Thermo Fisher Scientific, Waltham, MA). RNA quality control ...
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bioRxiv - Plant Biology 2024Quote: ... The RNA samples were subjected to quality control tests using a Ribo Green Assay (Invitrogen) for quantification and an Agilent Nano chip (Agilent Technologies ...
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bioRxiv - Plant Biology 2024Quote: Total RNA (1 μg) was treated with DNase I (Amplification grade, Invitrogen) at room temperature for 45 min and the enzyme was inactivated as per instructions of the manufacturer ...
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bioRxiv - Physiology 2024Quote: ... After blocking in UltraVision protein block (ThermoFisher Scientific) for 30min ...
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bioRxiv - Physiology 2024Quote: ... the calcium-free buffer was replaced by the liver digest media (17703-034, Thermo Fisher Scientific, Roskilde, Denmark) for 7 min ...
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bioRxiv - Physiology 2024Quote: ... 100ng of RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). After pre-amplification ...
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bioRxiv - Physiology 2024Quote: ... and incubated with a neutravidin-horseradish peroxidase (HRP, Thermo Scientific) solution at 1/2000 in TBS + 0.02% Tween 20 + 0.2% cold water fish gelatin for 1 hour ...
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bioRxiv - Physiology 2024Quote: ... and HRP activity was detected with a solution of 1% diaminobenzidine (Acros Organics) + 0.003% H2O2 in TBI ...
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bioRxiv - Physiology 2024Quote: ... Duplicates of the resultant cDNA were used for real time PCR using SYBR Master Mix (Applied Biosystems) and oligo primers for target genes and carried out on QuantStudio 3 (Applied Biosystems) ...
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bioRxiv - Physiology 2024Quote: ... / bromo-chloro-indolyl phosphate (Thermo Scientific) in AP buffer or using Naphtol AS-MX phosphate and Fast Red (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... according to the manufacture protocol and treated with recombinant Rnase-free DNAse I (Thermo Scientific). RNA concentration and purity were determined with NanoDrop 2000 Spectrophotometer (ND-2000 ...
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bioRxiv - Physiology 2024Quote: ... Total RNA from skeletal muscle was isolated using TRIzol reagent (Life Technologies) according to the manufacture protocol and treated with recombinant Rnase-free DNAse I (Thermo Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... namely a Thermo Q-Exactive Focus instrument (Thermo Fisher Scientific, USA) equipped with a Dionex Unimate 3000 UHPLC system was used ...
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bioRxiv - Plant Biology 2024Quote: ... Antibody binding was detected on X-ray film with Pierce ECL Substrate (Thermo-Fisher Scientific) or signals were directly captured in a G:BOX Chemi XRQ machine (Syngene ...
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bioRxiv - Physiology 2024Quote: ... qPCR was carried out with different TaqMan™ Gene Expression Assays (Applied Biosystems). Relative quantification of mRNA was performed according to the comparative 2-ΔΔCT method with SDHA as an endogenous control (see SI for detailed information).
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bioRxiv - Physiology 2024Quote: ... The nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI, 0.5 μg/ml, Thermo Fisher Scientific) and sections were mounted with ProLong Diamond Antifade Mountant (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2024Quote: ... Organoids were maintained and passaged every 6 days in organoid growth media (advanced Dulbecco’s modified Eagle’s medium/F12 supplemented with penicillin/streptomycin, 10 mM HEPES, Glutamax, B27 [Thermo Fisher Scientific] ...
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bioRxiv - Plant Biology 2024Quote: ... in a Thermal Cycling StepOnePlus (Applied Biosystems). Primers used ...
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bioRxiv - Plant Biology 2024Quote: ... All PCR reactions were performed on an Applied Biosystems Step One Plus System (Thermo Fisher Scientific). All PCR primers are listed in Supplemental Table S4 ...
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bioRxiv - Plant Biology 2024Quote: Probe-On Plus Slides (Fisher Scientific, Pittsburgh, PA, USA)
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bioRxiv - Physiology 2024Quote: ... stock solution in dimethyl sulfoxide (DMSO) (Thermo Fisher Scientific, Waltham, MA) was pipetted into the center of the sample droplet and mixed in with the pipette tip ...
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bioRxiv - Physiology 2024Quote: ... all samples were dehydrated with an increasing ethanol (Thermo Fisher Scientific, Waltham, MA) series (50 ...