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Citations for Thermo Fisher :
3051 - 3100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Cell suspensions were evaluated on an Attune NxT flow cytometer (ThermoFisher), and all analysis was performed in FCS Express (Version 7 ...
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bioRxiv - Physiology 2024Quote: ... The protein concentration was measured using the BCA method (23227; Thermo Fisher Scientific, USA). The protein samples were mixed with 5× sample buffer (MB01015 ...
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bioRxiv - Physiology 2024Quote: ... and goat anti-mouse (1:200; Invitrogen). All sections were counterstained with DAPI to identify nuclei ...
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bioRxiv - Physiology 2024Quote: ... Signals were detected by counter staining with the following antibodies: goat anti-guinea pig (1:400; Invitrogen), goat anti-rabbit (1:200 ...
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bioRxiv - Physiology 2024Quote: ... The hybridized sections were mounted using ProLong Gold Antifade mounting media (Thermo Fisher Scientific) and imaged using a Zeiss LSM800 microscope (Carl Zeiss ...
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bioRxiv - Physiology 2024Quote: ... Multivariable flow cytometry was performed using anti-mouse monoclonal antibodies: CD3-FITC (17A2; ThermoFisher), CD19-A700 (1D3) ...
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bioRxiv - Physiology 2024Quote: ... EVOS XL (Thermofisher Scientfic, UK) at 20x and 40x magnification ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated on a 4-12% Bis-Tris gel (Fisher Scientific) by gel electrophoresis and transferred onto a nitrocellulose membrane ...
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bioRxiv - Physiology 2024Quote: ... Nuclei were identified by staining with Syto83 fluorescent dye (0.2 µm concentration, Fluorescence 532, Thermo Fisher Scientific). The corresponding ROIs from the protein slide were selected and collected in a collection plate using a similar method as the RNA profiling ...
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bioRxiv - Physiology 2024Quote: ... and rabbit anti-CXCL10 (Invitrogen). Signals were detected by counter staining with the following antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... RNase free kit (Thermo Fisher Scientific, America) following the manufacturer’s instructions cDNA synthesis was performed according to the manufacturer’s instructions using the Invitrogen SuperScript IV kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Data was analysed with QuantStudio 6 and 7 Pro Real-Time PCR Systems Software (Thermo Fisher Scientific, America). For RT-qPCR ...
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bioRxiv - Plant Biology 2024Quote: FTIR spectra were collected on a Nicolet 6700 FTIR spectrometer (Thermo Scientific, Madison, WI, USA). The Smart iTR ATR sampling accessory was used ...
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bioRxiv - Plant Biology 2024Quote: ... Fast SYBR Green Master Mix (Thermo Fisher Scientific, Country) was used on the ViiA 7 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... was used on the ViiA 7 Real-Time PCR System (Thermo Fisher Scientific, America). Data was analysed with QuantStudio 6 and 7 Pro Real-Time PCR Systems Software (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was DNase treated with the Turbo DNA-free kit (Thermo Fisher Scientific). For quantitative real-time RT-PCR ...
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bioRxiv - Plant Biology 2024Quote: ... following the manufacturer’s instructions cDNA synthesis was performed according to the manufacturer’s instructions using the Invitrogen SuperScript IV kit (Thermo Fisher Scientific, America). To ensure equal quantities of RNA in each sample ...
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bioRxiv - Biophysics 2024Quote: ... Cell detachment for cryopreservation or experiments was achieved through 30 minutes of incubation with 5 mM EDTA in PBS without Ca+2 and Mg+2 (PBS-/-) followed by TrypLE Express dissociation agent (Gibco, 12605-010) for five minutes at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... Cells were cultured in hiPSC-RPE medium composed of MEM α (Thermo Fisher, 12549-089), 5% (vol/vol ...
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bioRxiv - Biophysics 2024Quote: ... 1% (vol/vol) N-2 supplement (Gibco, 17502-048), 55 nM hydrocortisone (Sigma-Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... 5% (vol/vol) knock-out serum replacement (Gibco, 10828-028), 1% (vol/vol ...
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bioRxiv - Plant Biology 2024Quote: ... PCR products were recombined into pDONR207 (Invitrogen) using Gateway® BP Clonase® II Enzyme mix (Invitrogen) ...
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bioRxiv - Plant Biology 2024Quote: ... using Gateway® BP Clonase® II Enzyme mix (Invitrogen). LR recombination reactions were performed using Gateway® LR Clonase® Enzyme Mix (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... BGLU3 and BGLU4 were created using the GATEWAY® Technology (Invitrogen). PCRs were performed with the Q5® High Fidelity DNA Polymerase (NEB ...
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bioRxiv - Plant Biology 2024Quote: ... To generate C-terminal fusion proteins with the C-terminal or N-terminal fragment of YFP, the genes were cloned into the expression vectors pEG100 (Early et al., 2006) using the Gateway technology (Invitrogen) (Gu and Innes 2011) ...
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bioRxiv - Plant Biology 2024Quote: ... The cDNA obtained served as a template for PCR amplifications using Platinum™ SuperFi II Green PCR Master Mix (Thermo Fisher Scientific, USA) and specific primers for ToBRFV LS PCR amplifications ...
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bioRxiv - Plant Biology 2024Quote: ... Samples (10 mg powder) were extracted threefold in ice-cold 90% (v:v) methanol (LC-MS grade; Fisher Scientific UK Limited or Th. Geyer GmbH & Co. KG), supplemented with luteolin 7-O-glucoside (Extrasynthese ...
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bioRxiv - Plant Biology 2024Quote: Total RNA (2μg) was extracted from the 24 treated leaves of H83 and L36 using the TRIzol kit (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... LR recombination reactions were performed using Gateway® LR Clonase® Enzyme Mix (Invitrogen) in order to transfer ORF sequences from Entry vectors to the pC2N1 allowing C-terminal translational fusion with the 10xMyc tag under the control of the 35S promoter (Bigeard et al. ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA isolation was performed by the Trizol method (Ambion) followed by RNA cleanup by the RNeasy mini kit (Quiagen) ...
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bioRxiv - Plant Biology 2024Quote: Protein identification was performed with Proteome Discoverer 2.0 (Thermo Scientific, USA). Protein identification threshold was established at 5% and 1% false discovery rates (FDR ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were incubated with anti-c-myc or anti-HA magnetic beads (Thermo Scientific) for 2-4 hours and washed with the same buffer without phosphatase inhibitors ...
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bioRxiv - Plant Biology 2024Quote: ... viral RNA served as a template for cDNA synthesis using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Fisher Scientific, USA) with the reverse primer R-6,392 ...
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bioRxiv - Plant Biology 2024Quote: Samples were analyzed using an ultra-high performance liquid chromatograph (Dionex UltiMate 3000, Thermo Fisher Scientific) and a quadrupole time-of-flight mass spectrometer (compact ...
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bioRxiv - Plant Biology 2024Quote: ... in H2OMilliQ (phase A) and 0.1% formic acid in acetonitrile (LC-MS grade; Fisher Scientific or HiPerSolv CHROMANORM, VWR) (phase B ...
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bioRxiv - Pathology 2024Quote: ... Antibodies to TOM70 (14528-1-AP, 1:200, Proteintech; Alexa Fluor 488-conjugated secondary antibody: A-11034, 1:500, Invitrogen), and troponin T (1:100 ...
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bioRxiv - Pathology 2024Quote: ... in culture medium (M199 Hanks (Gibco 12350-039), 5 mM creatine ...
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bioRxiv - Pathology 2024Quote: ... Protein concentrations were determined using Pierce BCA Protein Assay Kit (23225; Thermo Fisher Scientific). Equal amounts of protein from each sample were separated on SDS– polyacrylamide gels and transferred to PVDF membrane ...
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bioRxiv - Pathology 2024Quote: ... surface-coated four well Permanox slides (Thermo Fisher Sci, 177437) in culture medium (M199 Hanks (Gibco 12350-039) ...
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bioRxiv - Pathology 2024Quote: ... paraffin-embedded 5 µm LV cross-sections were incubated with Alexa Fluor 594-conjugated wheat-germ agglutinin (WGA, Thermo Fisher Scientific, Waltham, MA, USA). Images were acquired with a BZ-X 810 microscope (Keyence ...
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bioRxiv - Pathology 2024Quote: ... The gradient length of the Acclaim™ PepMap™ C18 2UM 75UMx500MM (Thermo Fisher Scientific, Dreieich, Germany) analytical column was adjusted to 187 min from 4 to 50% of 80% ACN and 0.08% FA at a flow rate of 300 nl/min ...
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bioRxiv - Pathology 2024Quote: ... and 3-hydroxybutyrate dehydrogenase 1 (15417-1-AP, Thermo Fisher Sci, Waltham, MA, USA). HRP-conjugated secondary antibodies (NA9340 ...
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bioRxiv - Pathology 2024Quote: ... coupled to an ESI-Orbitrap MS/MS (QExactive Plus, Thermo Fisher Scientific, Germany). The gradient length of the Acclaim™ PepMap™ C18 2UM 75UMx500MM (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: The peptides were analysed using a nanoLC (Ultimate 3000, Thermo Fisher Scientific, Germany) coupled to an ESI-Orbitrap MS/MS (QExactive Plus ...
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bioRxiv - Pathology 2024Quote: ... RT-PCR was carried out on StepOnePlus (Applied Biosystems, Foster City, CA, USA) using HotStarTaq DNA Polymerase (Qiagen ...
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bioRxiv - Pathology 2024Quote: ... Tomm70 mRNA expression was measured with TaqMan real-time PCR assay on StepOnePlus (Applied Biosystems, Foster City, CA, USA) using HotStarTaq DNA Polymerase (Qiagen ...
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bioRxiv - Pathology 2024Quote: ... AlexaFluor-conjugated secondary antibodies (ThermoFisher Scientific) used were for indirect immunolabelling at a concentration of 1:200 ...
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bioRxiv - Pathology 2024Quote: ... qPCR was carried out on StepOnePlus (Applied Biosystems, Foster City, CA, USA) using Power Sybr® Green qPCR Master Mix (4367659 ...
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bioRxiv - Pathology 2024Quote: ... Total DNA was isolated from murine frozen tissues using the Pure Link Genomic DNA Kit (Life Technologies, Carlsbad, CA, USA) following the manufactureŕs standard protocol ...
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bioRxiv - Pathology 2024Quote: ... using Power Sybr® Green qPCR Master Mix (4367659, Applied Biosystems, Foster City, CA, USA) and specific primers ...