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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... cell lysate supernatants were mixed with antibodies and recombinant protein G-Sepharose 4B beads (Thermo Fisher) at 4°C for overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysis was achieved via the M-PER™ Mammalian Protein Extraction Reagent (78503, Thermo Scientific), which was supplemented with Halt™ Protease Inhibitor Cocktail (87785 ...
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bioRxiv - Cell Biology 2020Quote: ... hTERT RPE1-FUCCI cells were co-transfected with TrueCut Cas9 protein v2 (Thermo Fisher Scientific, A36496) and the annealed tracrRNA:crRNA complex using the Lipofectamine CRISPRMAX Cas9 Transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Protein expression in Drosophila S2 cell lines was induced in Express Five SFM medium (Life Technologies) in the presence of 500mM CuSO4 at 25 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells in the plate were lysed for either protein extraction using RIPA buffer (Thermo Fisher Scientific) or RNA extraction using the Quick-RNA miniprep kit (Zymo Research ...
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bioRxiv - Neuroscience 2021Quote: Total protein extracts from harvested-cells were prepared in RIPA buffer (Thermo Scientific, Waltham, MA, USA) containing phosphatase and protease inhibitors (Sigma-Aldrich) ...
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bioRxiv - Genomics 2021Quote: ... Cleared cell lysates were subjected to IP with CTCF antibody-bound Protein G Dynabeads (Invitrogen, 10004D) overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were lysed for 15 min using B-PER Bacterial Protein Extraction Reagent (Thermo Scientific) containing DNase I (New England Biolabs ...
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bioRxiv - Immunology 2020Quote: Proteins derived from cell lysates were separated on SDS-PAGE gel (4 to 12%) (Novex, Invitrogen) with 2-(N-morpholino ...
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bioRxiv - Microbiology 2020Quote: ... All proteins were expressed in Escherichia coli one-shot BL21star (DE3) cells (Invitrogen, Carlsbad, CA, USA), as previously described [13] ...
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bioRxiv - Immunology 2020Quote: The S protein and RBD/S1B constructs were expressed by transient transfection of Expi293F cells (Gibco) and purified from clarified transfected cell supernatants four days post-transfection using HisTrap FF and HisTrap HP columns (GE Healthcare Life Sciences) ...
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bioRxiv - Molecular Biology 2021Quote: RBD proteins were produced in Expi293F cells grown in suspension using Expi293F expression medium (Life Technologies) at 33C ...
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bioRxiv - Immunology 2020Quote: ... Cell pellets were lysed in 50 μL of Mammalian Protein Extraction Reagent (MPER; Thermo Scientific, 78503). Conjugate DNA was measured by qPCR amplification from 1 μL of lysate in a 10 μL reaction containing 5 μL of iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: Cell proteins were extracted using RIPA lysis buffer (Catalog no. 89900) from Thermo Fisher (Winnipeg, MB). The total protein levels were measured by the Bradford method [16] ...
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bioRxiv - Cell Biology 2022Quote: ... the concentrations of cell lysate were measured using Micro BCA Protein Assay Kit (Thermo Fisher Scientific). Cell lysates and anti-c-Myc magnetic beads (Cat ...
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bioRxiv - Microbiology 2022Quote: ... the cells were lysed in mammalian protein extraction reagent (M-PER; Cat# 78503, Thermo Fisher Scientific) supplemented with Halt protease inhibitor cocktail (Cat# 877886 ...
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bioRxiv - Cancer Biology 2024Quote: Tissues and cell protein lysates were prepared with RIPA buffer (89900, Thermo Fisher Scientific, Waltham, MA), which includes a protease inhibitor cocktail (05892791001 ...
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bioRxiv - Biophysics 2022Quote: All protein samples used for structural and functional analyses were prepared using Drosophila S2 cells (Invitrogen) or high-expression clones for each protein sample established from S2 cells in previous studies (Nuemket et al. ...
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bioRxiv - Biophysics 2022Quote: ... The protein expression was done in Escherichia coli BL21(DE3) pLysS cells (Invitrogen, Grand Island, NY). Samples for MAS ssNMR were uniformly 13C ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were homogenized in T-PER™ tissue protein extraction reagent (Thermo Fisher Scientific, Cat# 78510) containing Triton(R ...
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bioRxiv - Biophysics 2023Quote: ... which was performed using a Pierce cell surface protein biotinylation and isolation kit (Thermo Scientific, A44390).
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bioRxiv - Immunology 2024Quote: ... BA.2.86 and H-CoV S proteins were transiently transfected in ExpiCHO-S cells (Thermo Fisher) using ExpiFectamine™ CHO Reagent ...
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bioRxiv - Immunology 2024Quote: ... single cells were stimulated with a cytokine stimulation cocktail containing protein transport inhibitors (Thermo Fisher Scientific) for 2 hours at 37C in RPMI supplemented with 10%FCS ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant hTMEM87A proteins (WT and mutants) were transiently expressed in Expi293F cells (#A14527, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were lysed for 5 min with M-PER mammalian protein extraction reagent (Thermo Scientific) including Halt protease inhibitor (Thermo Scientific ...
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bioRxiv - Pathology 2023Quote: ... Cells lysates were centrifuged and protein concentration was measured using a BCA assay kit (Thermo Scientific). Twenty μg of protein was loaded onto a 4-12% Bis-Tris gradient gel (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Whole cell lysates were prepared in M-PER buffer (1x Mammalian Protein Extraction Reagent (Thermo Scientific), 0.1% Halt Protease & Phosphatase inhibitor (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Total cell lysates in CHAPS buffer were quantified by BCA protein assay kit (Fisher Scientific #10678484) (except for co-IP samples) ...
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bioRxiv - Bioengineering 2023Quote: Host cell Protein were analyzed using ProteinSEQ™ CHO HCP Quantitation Kit (Thermo Fisher Scientific, USA) as per supplier’s protocol ...
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bioRxiv - Microbiology 2023Quote: Cells were washed with PBS and lysed in Tissue Protein Extraction Reagent (T-PER, Thermo Scientific) containing protease inhibitors (cOmplete tablets EasyPack ...
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bioRxiv - Cell Biology 2023Quote: ... The cell extracts were pre-cleaned with protein A/G magnetic beads (88804, Thermo Fisher Scientific) at 4℃ for 60 min ...
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bioRxiv - Biophysics 2023Quote: Recombinant hTMEM87A proteins (WT and mutants) were transiently expressed in Expi293F cells (#A14527, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Protein constructs were expressed in HEK293T cells using Lipofectamine 2000-mediated (Invitrogen, Catalog #11668030; Waltham, MA) plasmid transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Whole cell lysates were prepared in M-PER buffer (1x Mammalian Protein Extraction Reagent (Thermo Scientific), 0.1% Halt Protease & Phosphatase inhibitor (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations in cell lysates were determined using the bicinchoninic acid (BCA) assay (ThermoFisher Scientific #23227). For analysis of PrPC levels ...
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bioRxiv - Molecular Biology 2023Quote: ... Whole cell lysates were prepared in M-PER buffer (1x Mammalian Protein Extraction Reagent (Thermo Scientific), 0.1% Halt Protease & Phosphatase inhibitor (Thermo Scientific ...
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bioRxiv - Physiology 2024Quote: ... Total cell lysate protein concentrations were determined with the Better Bradford Kit (23236; Thermo-Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: Cell lysates were incubated with anti-FLAG antibody along with Protein G-conjugated Dynabeads (10004D, Invitrogen) at 4°C for 4 hours ...
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bioRxiv - Physiology 2024Quote: Cells were scraped in ice cold mammalian protein extraction reagent (MPER) (ThermoFisher Scientific, Waltham, MA, USA). Cell lysates were sonicated on ice twice for 3 second intervals before being diluted 1:10 in type 1 water ...
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bioRxiv - Genetics 2024Quote: ... cell lysates were pre-cleared with 5 µl of unbound protein G magnetic beads (10004D, Invitrogen) for 30 min with rotation at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Co-IP was performed on cell lysate supernatants by Dynabeads Protein G Immunoprecipitation kit (10007D, Invitrogen). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the cell lysate was also determined using a BCA assay (Thermo Scientific) according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... Procaspase-3 (0.123 mg/mL protein extract) and caspase-3 (1.0 U) were assayed in 96-well microplates (Nunc™ MicroWell™ 96-Well, Thermo Scientific™, ThermoFisher Scientific, Porto Salvo, Portugal) using 100 μL of 20 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subject to reducing SDS-PAGE and immunoblotted using antibodies recognizing recombinant protein (anti-NRG1 NRG1abcam ab180808 1:5000 dilution in 5% milk) or Lamin A (Invitrogen MA1-06101, 1:1000 in 3% BSA).
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations of the subcellular protein fraction lysates were measured using Pierce BCA Protein Assay Kit (ThermoFisher). Samples were boiled for 10 minutes and the extracts were separated by SDS-polyacrylamide gel electrophoresis using precast 4–20% Precise protein gels (ThermoScientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Rab27b protein levels were normalized to total protein levels determined by SYPRO Ruby protein gel stain (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: The protein–protein interaction for endogenous protein was studied by using the Co-Immunoprecipitation Kit (Thermo Scientific). For overexpressed HA-tag proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Synaptosomal protein expression levels were normalized to total protein using No-Stain Protein Labeling Reagent (Invitrogen, A44449). Primary antibodies (Anti-Shank3 (N367/62) ...
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bioRxiv - Microbiology 2023Quote: ... antibody-protein complexes were deposited on 25µL of protein A magnetic beads (Dynabeads protein A, Life Technologies) and incubated for 4h at 4°C on the rotator ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were assayed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™ ...