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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was quantified using Qubit™ Protein and Protein Broad Range (BR) Assay Kits (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... and protein concentration was determined by protein assay (EZQ Protein Quantification Kit; Life Technologies, Carlsbad, CA). Ct226-FLAG was affinity purified using magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: Beads were first activated with 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (Thermo Fisher Scientific) in the presence of N-hydroxysuccinimide (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: Caspase-3 activity was determined using EnzChek™ Caspase-3 Assay Kit #2 (Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... (75 mm i.d. 3 2 cm, Acclaim PepMap100 C18 3 mm, 100 A°, ThermoFisher Scientific) and separated over an EASY-Spray column ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The caspase-3 activity was quantified using EnzChek™ Caspase-3 Assay Kit #1 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) was obtained from Life Technologies (Carlsbad, CA) and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Neuroscience 2022Quote: RNA was 3’ labelled with biotin using Pierce™ RNA 3’ End Biotinylation Kit (ThermoFisher) and purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... Head kidney pieces (3 mm × 3 mm) were collected in RNA later (Thermo Fisher Scientific), transferred to 500 μL MagNA Pure LC RNA Isolation Tissue Lysis buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Gdf1/3 and Gdf1/3-like mRNA were synthesized using T7 mMESSAGE mMACHINE kit (Ambion). Unfertilized eggs were injected with mRNAs and fertilized as previously described50 ...
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bioRxiv - Biophysics 2023Quote: ... 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine (DiI) and 1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindodicarbocyanine (DiD)) were purchased from ThermoFisher Scientific and used without additional purification ...
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bioRxiv - Biophysics 2024Quote: ... and 250 µM of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (Thermo Scientific, 24510) in Borate buffer (100 mM Borate-NaOH ...
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bioRxiv - Cell Biology 2019Quote: Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were cultured on Vitronectin (VTN-N) recombinant human protein (ThermoFisher Scientific, A14700). Culture vessels were coated with 200μL/cm2 of Vitronectin that had been diluted to 6μg/ml with PBS and incubated at 37°C for at least 1 hour ...
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bioRxiv - Biophysics 2021Quote: A commercial HEK cell suspension cell line optimized for protein expression Expi293-F (Cat. No. A14635, Gibco Life Technologies, Carlsbad, California, USA) was used according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: Proteins from MACsorted cells were extracted during 30 minutes at 4°C in RIPA buffer (ThermoFisher; 50 µL per million cells, 89901) supplemented with Halt™ Protease Inhibitor Cocktail (100X ...
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bioRxiv - Physiology 2022Quote: ... Extraction of cell membrane and cytoskeleton fractions was carried out using the Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific, #78840) in line with the manual.
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bioRxiv - Cell Biology 2020Quote: To compare the presence of the nucleolar rim of mNG-tagged proteins in live and fixed cells we fixed mNG-tagged cells in 4% formaldehyde (Thermo Scientific, 28908).
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bioRxiv - Molecular Biology 2024Quote: ... Cortical neurons were plated at a density of ∼500 cells/mm2 (for RNA or protein isolation) or ∼250 cells/mm2 (for immunocytochemistry) on tissue culture plates or coverslips (Fisher Scientific, NC0672873) coated with 0.1mg/mL poly-L-lysine (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... Consensus and Vietnam matrix protein 2 (M2)-inducible HEK cells were generated using the Flp-In™ T-REx™ 293 Cell Line system (Invitrogen) and grown following the methods in the previous papers 53 ...
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bioRxiv - Immunology 2024Quote: ... cells were re-suspended in 200 μL of cell stimulation cocktail with protein transport inhibitors (Thermo-Fisher Scientific, Cat: 00-4975-03) in CCM for 2 hours at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids for expression of sACE2-sfGFP protein were transfected in to Expi293F cells using Expifectamine (ThermoFisher) according to the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2020Quote: ... lysates (~5 × 106 cells per experiment) were pre-cleared with PierceTM Protein A Agarose (Thermo Scientific) for 2 hr at 4°C ...
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bioRxiv - Genomics 2020Quote: The total protein was extracted from log-phase cells with NuPAGE LDS sample buffer (ThermoFisher NP0007) after 0.2 N NaOH treatment for 5 min (Kushnirov ...
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bioRxiv - Immunology 2021Quote: ... 2mM L-glutamine and 2μl/ml solution of cell stimulation cocktail and protein transport inhibitors (Invitrogen eBioscience™ Cell Stimulation Cocktail plus protein transport inhibitors (500X)) ...
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bioRxiv - Bioengineering 2019Quote: ... The protein content of HCCF and cells was measured using a Nanodrop 2000 (Thermo Fisher Scientific). Before analysis ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lysates (15-20 ug of protein) were resolved in 4%-12% Bis-Tris gels (Invitrogen) and transferred to PVDF membranes using wet transfer ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 38] and purified from the cell culture medium to homogeneity by Protein A chromatography (Thermo Scientific). Quality control of the proteins was performed by SDS-PAGE and size exclusion chromatography (Superdex200 10/300GL ...
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bioRxiv - Systems Biology 2021Quote: ... cells were lysed using M-PER or Mem-PER mammalian protein extraction reagents (Thermo Fisher Scientific) and blotted onto PVDF membranes as described above ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue or cells were lysed in N-PER™ Neuronal Protein Extraction Reagent (#87792, Thermo Fisher) supplemented with protease and phosphatase inhibitor cocktail (#78440 ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue or cells were lysed using N-PER™ Neuronal Protein Extraction Reagent (#87792, Thermo Fisher) supplemented with protease inhibitor cocktail (#5871S ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitated proteins from cells synchronized in G2 were analyzed on a Q-Exactive Plus (Thermo Scientific) mass spectrometer that was coupled to an EASY nLC 1200 UPLC (Thermo Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified cell supernatant containing recombinant mAb was passed over Protein A Agarose resin (Thermo Fisher Scientific). Protein A resin was extensively washed with 25 mM Phosphate pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... The cell lysate was then quantified for protein using a BCA Assay Kit (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... cells were extensively washed with PBS and stained with NeutrAvidin Protein DyLight 488 (Invitrogen, cat: 22832) 1:200 for 20 min prior analysis using an Accuri C6 Flow Cytometer (BD Biosciences ...
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bioRxiv - Systems Biology 2020Quote: ... cells were extensively washed with PBS and stained with NeutrAvidin Protein DyLight 488 (Invitrogen, cat: 22832) 1:200 for 20 min prior analysis using an Accuri C6 Flow Cytometer (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell extracts were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris protein Gels, Thermofisher), followed by transfer to nitrocellulose membrane using wet transfer at 30 V overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations from whole cell lysates were determined using a Bradford reagent (Thermo Fisher, Carlsbad, CA). Protein samples (50µg total protein ...
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bioRxiv - Biophysics 2020Quote: ... Fusion protein expression plasmids were transfected into (70-90% confluent) cells using Turbofect™ (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... cytosolic RNAs were isolated from cell lysates using the subcellular protein fractionation kit (Thermo Fisher Scientific) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ERα proteins were immunoprecipitated from J774a.1 cell lysates according to the manufacturer’s protocols (11204D, Invitrogen). The ERα antibody was the same as that used for Western blotting and was purchased from Santa Cruz (1:200 dilution) ...
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bioRxiv - Molecular Biology 2020Quote: Cells were lysed in RIPA buffer and protein concentration was measured using BCA kit (Thermo Fisher). Protein lysates were resolved on 8-10% gels and transferred to PVDF membrane ...
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bioRxiv - Molecular Biology 2020Quote: Cell lysate total protein was determined by using the Pierce Bicinchoninic acid (BCA) assay (Thermo Scientific), following the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... whole-cell protein extracts were prepared with RIPA buffer containing a protease inhibitor cocktail (Thermo Scientific). Proteins were separated by NuPAGE 4-12% Bis-Tris Gel (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The protein with a C-terminal Strep-tag was expressed in Expi293 cells (Thermo Fisher Scientific) and subsequently purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences ...
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bioRxiv - Immunology 2019Quote: ... The colon epithelial cells were lysed in situ with tissue protein extraction reagent (TPER, Thermo Fisher) containing protease inhibitor cocktail ...
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bioRxiv - Synthetic Biology 2019Quote: ... Whole cell extracts were prepared with B-PER Complete Bacterial Protein Extraction Reagent (Thermo Fisher Scientific). Western blot analysis was conducted as described previously 7 ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested and lysates prepared using M-PER mammalian protein extraction reagent (Thermo Fisher Scientific), and protein concentration was measured using a bicinchoninic acid (BCA ...
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bioRxiv - Cancer Biology 2019Quote: ... the cells were collected and lysed with T-PERTM Tissue Protein Extraction Reagent (Thermo Scientific, USA) containing protease inhibitor and phosphatase inhibitor (Roche ...
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bioRxiv - Immunology 2020Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermofisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS 13 ...
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bioRxiv - Molecular Biology 2020Quote: ... For each immunoprecipitation experiment 60×106 cells and 60 μl of Protein G Dynabeads (Invitrogen, #10004D) coupled with 6 μl of the FLAG M2 antibody (Sigma-Aldrich ...