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Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The presence of engineered mutations was confirmed by Sanger sequencing following extraction of RNA from virus stocks with TRIzol (Invitrogen) and cDNA amplification using a OneStep RT-PCR Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2019Quote: vRNA was extracted from virus supernatant containing the same amount of PFU using PureLink Viral RNA/DNA Mini Kit (Invitrogen). vRNA was reverse transcribed with Uni12/13 specific primers using Superscript IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... filled with the infusate (virus particles suspended in a solution of phosphate-buffered saline (PBS) and Oregon Green 488 (Invitrogen)) into the centers of the craniotomies ...
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bioRxiv - Molecular Biology 2019Quote: iPSCs were generated by transducing control and FXS patient fibroblasts with Sendai virus from the Cytotune 2.0 Reprogramming Kit (Life Technologies) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: Fibroblasts isolated either from human fetal liver tissues or human adult skin after collagenase dissociation were reprogrammed into iPSCs with the integration-free based Sendai virus (Cytotune 2.0 kit from Life Technologies). Fibroblasts were used at low population doubling (less than 5 ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription was performed to convert RNA into cDNA using the Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA samples were reverse transcribed (1µg of total RNA/sample) using the Moloney Murine Leukemia Virus (MML-V) enzyme (Life Technologies) and Oligo-dT Primers ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed as previously described(42) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2021Quote: ... that co-localises with IBDV virus factories (16) and a goat anti-mouse secondary antibody conjugated to Alexa-Fluor 488 (Invitrogen). Wells were scored as positive or negative based on the presence of infected cells ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed as previously described [39] using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2021Quote: ... Ten-fold serial dilutions (1×10−1 to 1×10−6) of virus stocks were prepared in MEM (supplemented with 25 mM HEPES (Gibco), 2mM L-Glutamine (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... 1µg was used for cDNA synthesis using a Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (SuperScript II from ThermoFisher Scientific) and random primers ...
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bioRxiv - Cell Biology 2021Quote: ... and 4 μg of plasmid VSV-G encoding G glycoprotein of vesicular stomatitis virus(VSVG) using Lipofectamine 3000 Reagent (Invitrogen). 12 h later ...
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bioRxiv - Pathology 2021Quote: ... 10 µl of rSARS-CoV-2 NL virus stock was diluted in 10 ml of OptiMEM media (ThermoFisher Scientific, #51985091) and then added to cells ...
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bioRxiv - Microbiology 2019Quote: ... 200 ng of RNA from each sample was used to generate virus cDNA using in a Maxima H minus strand kit (Invitrogen) and ZIKV specific primers (Supplemental Table 6) ...
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bioRxiv - Microbiology 2020Quote: ... Vero FM cells seeded in 24-well plates were infected in duplicate with 100 μl 2-fold serial dilutions of patient material in a total volume of 200 μl using virus isolation medium [DMEM (Gibco) containing ...
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bioRxiv - Immunology 2020Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of nucleocapsid (N ...
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bioRxiv - Microbiology 2021Quote: ... Primary cells were immortalized by transfection of pRSVAg1 plasmid expressing Simian Vacuolating Virus 40 large T antigen (SV40T) with lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... 21] or a Sendai virus reprogramming kit (Cyto Tune 2.0 Sendai reprogramming vectors, Ref#A16517; Lot#A16517, Invitrogen, Carlsbad, CA). hiPSCs were cultured on hESC-qualified Matrigel® (Corning ...
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bioRxiv - Microbiology 2021Quote: ... The attachment of HIV-1 virus produced in the presence of TMEM123 was also separately tested by pre-incubating the virus with mouse monoclonal anti-TMEM123 antibody (297617) (ThermoFisher) (25ug/ml ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 until the following day. Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat. no. 61965026 supplemented with 2% FBS (Gibco, Cat ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
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bioRxiv - Neuroscience 2022Quote: Total RNA isolation from the AAV9 virus injected region of the left frontal cortex was performed as previously described [40] by TRIzol LS Reagent (10296010, Invitrogen). For cDNA synthesis ...
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bioRxiv - Plant Biology 2022Quote: ... was transcribed following DNase I treatment using Moloney Murine Leukemia Virus reverse transcriptase and an oligo dT(18) primer according to the manufacturer’s protocol (ThermoFisher Scientific). qPCR analysis was carried out in a CFX96-C1000 96-well plate thermocycler (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 until the following day. Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat. no. 61965026 supplemented with 2% FBS (Gibco, Cat ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µg of total RNA was subjected to random reverse transcription using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV-RT; Invitrogen) and random primers (Roche) ...
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bioRxiv - Systems Biology 2024Quote: ... 1 million 293T STING-mNG or 0.5 million BJ1 seeded the same day in one well of a 6 well plate were transduced with 2ml of virus including 8µg/ml Protamine (Thermo Fisher). Cells were incubated with viral supernatant for 1-2 days and cells were then transferred in a fresh flask for selection ...
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bioRxiv - Developmental Biology 2024Quote: ... A total of 1 µg of RNA was used as a template for cDNA synthesis using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT) (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... PBMCs were reprogrammed into iPS cells using transient overexpression of pluripotency-associated factors (OCT4, KLF4, and SOX2) via non-integrating Sendai virus particles (Cytotune 2.0 kit, Life Technologies). At least five clones per TSC2+/+ and TSC2+/− donor were established into iPS cell lines ...
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bioRxiv - Genetics 2024Quote: ... The iPSCs were made Sendai virus free by keeping in culture and testing for the presence of Sendai Virus (SeV) at different times by measuring SeV levels in total RNA using RT-qPCR with FAM labeled SeV (Thermofisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... cDNA of the virus genome was generated by reverse transcription with random hexamer as the primer (SuperScript III, ThermoFisher Scientific). The HA1 subunit was divided into three fragments for PCR (PrimeSTAR Max DNA Polymerase ...
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bioRxiv - Biochemistry 2023Quote: ... Concentrated virus was applied through a 0.45 μm surfactant free cellulose acetate syringe filter (Nalgene, Fisher Scientific #09-740-106) and immediately used or stored at -80ºC for up to one month ...
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bioRxiv - Microbiology 2023Quote: ... generated by Knut Falk in the late 1990s) or mouse IgG2a targeting influenza virus NP (clone D67J, Thermo Fisher Scientific) (both ...
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bioRxiv - Synthetic Biology 2023Quote: ... The virus concentration using Lenti-X Concentrator was performed with the manufacturer’s protocol and dissolved in Opti-MEM (Gibco #31985062) to yield a 10- or 15-fold concentration ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR was performed according to the manufacturer’s protocol using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher). Fluorescent signals were acquired using QuantStudio 6 Real-time PCR system (Thermo Fisher).
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bioRxiv - Microbiology 2023Quote: ... Lungs from K18-ACE2 C57BL/6 mice infected with virus were collected at indicated time points and were homogenized in Trizol (Invitrogen) and RNA was isolated using manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR was performed with 1 µg of RNA using 100 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen) according to instruction of the manufacturer ...
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bioRxiv - Pathology 2023Quote: ... Total RNA was extracted from clinical specimens (swabs and tissues) and virus isolates using the MagMax AM1836 96 Viral RNA Isolation Kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... were co-transfected with vesicular stomatitis virus G (600 ng) and pSPAX2 (800 ng) using Lipofectamine 3000 (Cat# L3000001, ThermoFisher). The lentiviral supernatants were collected 2 days after transfection ...
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bioRxiv - Biochemistry 2024Quote: ... Ten micrograms of total RNA was reverse transcribed (RT) at 37 °C using random hexamer primers and Moloney murine leukemia virus retrotranscriptase (Invitrogen-Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: We established mouse iPSC lines (CD1 iPSC or ntdTomato iPSC) using Sendai virus-mediated reprogramming kit: CytoTune2.0 (Thermo Fisher, A16517) and followed manufactured protocol as described before24 ...
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bioRxiv - Neuroscience 2024Quote: ... sections of virus-injected mice were incubated in a solution of chicken anti-eGFP (1:2000, Thermo Fisher Scientific, A10262) and mouse anti-gephyrin (1:100 ...
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bioRxiv - Immunology 2021Quote: ... and the extracted plasmid was then transfected into HEK293F cells at a density of 3×106 cells/ml using PEI (Invitrogen, Thermo Fisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBD was harvested 72 h after infection ...
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bioRxiv - Cancer Biology 2023Quote: ... We then used 10–50 mg of tumor samples and ← 3 · 105 of cell line cells for RNA extraction with the TRI-zol Reagent (Thermo Fisher Scientific, Waltham, MA USA) according to manufacturers protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A-tagged proteins were detected with HRP-conjugated polyclonal anti-Protein A (Invitrogen, PA1-26853) at 1:5000.
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were resolved alongside a protein ladder (PageRuler Plus Pre-stained Protein Ladder, Thermo Fisher Scientific) on NuPAGE 4-12% BisTris gradient gels using NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific) ...