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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitated proteins were collected on Protein-G Dynabeads (Invitrogen #10004D), separated by SDS-PAGE (Mini PROTEAN TGX ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was quantified using the BCA protein assay (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: 10 µl of Protein A beads (DynabeadsTM Protein A; Invitrogen) equivalent to 10 µl bed volume of slurry were prepared per each ChIP reaction ...
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bioRxiv - Immunology 2023Quote: ... protein was quantified by Pierce BCA Protein Assay (ThermoFisher #23225), XT sample buffer + reducing agent ...
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bioRxiv - Immunology 2023Quote: ... Protein concentration was determined using EZQ protein quantitation kit (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were quantified by BCA protein assays (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2024Quote: ... After protein quantification with BCA protein assay kit (23225, ThermoFisher), samples were mixed with SDS gel loading buffer (L-4390 ...
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bioRxiv - Cancer Biology 2024Quote: ... and protein quantified by Pierce BCA Protein Assay (Thermo Scientific). Protein samples were diluted to equal concentrations in lysis buffer and boiled for 5 minutes in 1X Laemmli Sample Buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were determined by BCA protein assay (Thermo Scientific). Proteins were resolved with SDS-PAGE utilizing 4–15% Mini-PROTEAN TGX Precast Gels (Biorad ...
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bioRxiv - Immunology 2024Quote: ... Protein concentration was determined using EZQ protein quantitation kit (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using EZQ protein quantitation kit (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined by BCA Protein Assays (Thermo Scientific) 30 μg of total lysate were separated by 4–12% NuPage Bis-Tris PAGE (Invitrogen ...
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bioRxiv - Systems Biology 2024Quote: ... protein concentration was measured by Pierce 660nm Protein Assay (ThermoFisher). The volume corresponding to 100 μg protein was transferred to a 96-well 2 ml deep-well plate (Eppendorf ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were induced to produce mov10-YFP by supplementation of the medium with 1 µg/ml doxycycline and then infected with sindbis virus (SINV-mCherry, see below [33]) and then incubated with MitoTracker Deep Red (Thermo Fisher) at 125 nM for 30 minutes before being plunge frozen as above for imaging.
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bioRxiv - Molecular Biology 2021Quote: ... and puromycin resistance marker-Bovine Growth Harmone polyA-signal (amplified from pMK232 (Natsume et al., 2016)) or blasticidin resistance marker-Simian Virus 40 polyA-signal (amplified from pcDNA6/TR (Thermo Fisher)) were cloned into pTwist-Amp (Twist Bioscience ...
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bioRxiv - Molecular Biology 2021Quote: ... was performed using double nucleic acid detection kit for respiratory syncytial virus (A/B) (Shenzhen shengkeyuan) with the ABI Q7 (Applied Biosystems). The reaction was conducted according to the manufacturer’s instructions with total volume of 20 μL.
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bioRxiv - Immunology 2022Quote: ... Two micrograms of liver RNA per sample was used for qRT-PCR analysis for Plasmodium 18S rRNA using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). Data were normalized for input to the GAPDH control (hepatocytes ...
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bioRxiv - Immunology 2021Quote: ... The isolated RNA was then evaluated in RT-qPCR using the TaqMan Fast Virus 1-step Master Mix (Thermo Fisher Scientific) on a QuantStudio Flex 6 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... and infected at a MOI of 1 with the VSV-ΔG-Fluc-2A-GFP/VSV-G virus diluted in 10mL Opti-MEM (Life Technologies). The cells were incubated 1 hour at 37C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ZIKV RNA samples were each measured in triplicate on an Applied Biosystems ViiA 7 machine using a Taqman Fast Virus 1-Step MasterMix (Thermo Fisher) with primers ZIKV 1087 forward (CCGCTGCCCAACACAAG) ...
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bioRxiv - Microbiology 2020Quote: ... 24 h later the dish was washed with PBS and treated with PR8 virus (MOI =2) in the infection medium [F-12K with 2% BSA fraction V (Thermo Fisher), 1% antibiotic-antimycotic (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 ml microcentrifuge polypropylene tubes containing the virus (500 µL total volume) were exposed to direct heat in a heat block (Fisher Scientific). After heating ...
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bioRxiv - Immunology 2021Quote: ... The mouse organ tissues were homogenized to detect the virus gene copy number by qPCR (TaqMan Universal Master Mix II with UNG, 4440044, Applied Biosystems). The sequences of the primers are listed in Table 2.
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bioRxiv - Microbiology 2021Quote: ... were seeded in 96-well plates 48h prior to virus inoculation at a density of 5×104 in DMEM (Gibco #41965039) supplemented as described above ...
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bioRxiv - Neuroscience 2022Quote: LC-MS/MS analyses were conducted using either a QExactive Plus Orbitrap (QE, RNase-digested polysomes) or a Velos Pro Elite Orbitrap (Elite, virus polysomes and HEK293 aggregates) mass spectrometer (Thermo Fisher) coupled online to a nanoAcquity UPLC system (Waters Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... the unbound virus was removed by washing with infection media followed by agarose overlay with 1X MEM Temin’s modification (Thermo Fisher, 11935046) +0.3% agarose ...
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bioRxiv - Microbiology 2022Quote: Viral RNA in apical secretions was quantified using the TaqMan™ Fast-Virus-1 Step master mix (Thermo Fisher, Basel, Switzerland) with these cycling parameters ...
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bioRxiv - Microbiology 2022Quote: ... HuNoV RNA genome equivalents (GEs) were determined by reverse transcription-quantitative PCR (RT-qPCR) analysis using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and GII specific primer/probe sets (Kageyama et al. ...
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bioRxiv - Bioengineering 2022Quote: ... infected cells were counted and determined by evaluating GFP expression after 24 hours exposure to virus-antibody mixture using Attune NxT Acoustic Focusing Cytometer (Thermo Fisher). Half-maximal inhibitory concentration (IC50 ...
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bioRxiv - Plant Biology 2021Quote: ... and then incorporated into the destination vector pMV3 (driven by the cauliflower mosaic virus (CaMV) 35S promoter) using the LR recombination reaction (Invitrogen, USA). All the recombinant constructs were transformed into the Agrobacterium tumefaciens strain C58 by electroporation and subsequently transformed into the tomato genome via explants of cotyledon ...
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bioRxiv - Developmental Biology 2021Quote: ... The virus was spun at 500 g for 5 min and the supernatant filtered through a 45 μm polyethersulfone filter (Fisher Scientific) and subsequently concentrated ...
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bioRxiv - Molecular Biology 2020Quote: ... Sf9 transfection with bacmid DNA and virus amplification were performed essentially as described for the Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). Sf9 cells were maintained in Hyclone CCM3 (CCM3 liquid medium with L-glutamine ...
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bioRxiv - Immunology 2019Quote: ... and cDNA was reverse transcribed using the Moloney murine leukemia virus reverse transcriptase and random hexamer oligonucleotides for priming (Life Technologies). The expression of CPT1 was determined using PCR SYBR Green sequence detection system (Eurogentec ...
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bioRxiv - Neuroscience 2019Quote: cDNA sequences from the leader through the first half of the rabies virus P gene were amplified using Platinum SuperFi Green Master Mix (Invitrogen (Thermo Fisher), USA ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and PCR amplification with virus candidate specific primers using the Platinum™ SuperFi™ DNA polymerase (Invitrogen™, Catalog number: 12359010). Amplified products were Sanger sequenced using intermediary primers enabling a full-length coverage (all primers are listed in Table S5).
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at approximately 250 × g for 5 min at 4 °C and resuspended in thawed Geltrex™ LDEV ([lactose dehydrogenase elevating virus]-Free Reduced Growth Factor Basement Membrane Matrix; Gibco). Two 15-μl drops of the matrix-cell suspension were pipetted into wells of a Nunc™ 24-well-Nuclon Delta-treated plate (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied BiosystemsTM TaqManTM Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...
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bioRxiv - Plant Biology 2021Quote: ... The identified gene fragments were then PCR-amplified from whitefly cDNA and cloned into a binary tobacco rattle virus VIGS vector pTRV2 (Liu et al., 2002) using the Invitrogen Gateway recombination cloning technology (Invitrogen, USA). As a positive control ...
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bioRxiv - Microbiology 2020Quote: ... apical poles were gently washed with warm PBS and then infected with a 150-μL dilution of HRSV-A (Long) virus in OptiMEM medium (Gibco, ThermoFisher Scientific) at a MOI of 1 ...
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bioRxiv - Microbiology 2020Quote: Zika virus RNA yields were assessed using real-time PCR on a 7500 Fast Real-Time PCR instrument (Thermo Scientific, Poland). ZIKV cDNA was amplified in a reaction mixture containing 1× TaqMan Universal PCR Master Mix (RT-PCR mix ...
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bioRxiv - Developmental Biology 2019Quote: Peripheral blood CD34+CD38- cells mobilized from healthy donors were reprogrammed into iPSCs (MCND-TENS2 line) using the integration-free CytoTune 2.0 Sendai virus reprogramming kit (A16517, Thermo Fisher Scientific) following the method previously reported30,31 ...
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bioRxiv - Neuroscience 2020Quote: Total RNA (~40 μg per reaction) was reverse-transcribed and PCR amplified using Taqman Fast Virus 1-Step Master Mix (Applied Biosystems) and primetime assays for specific genes (IDT) ...
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bioRxiv - Microbiology 2020Quote: SADS-CoV spike glycoprotein (virus strain GDS04; GenBank No.: ASK51717.1) gene was synthesized with codons optimized and inserted into pFastBac vector (Life Technologies Inc.). The ectodomain of SADS-CoV spike protein without the transmembrane anchor and intracellular tail (residues 18-1068 ...
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bioRxiv - Immunology 2020Quote: ... Ebola virus Makona Glycoprotein (EBOV-GP) (KM233102.1) was synthesized and cloned into pcDNA3.1 by GeneArt AG technology (Life Technologies, Regensburg, Germany) or VSV-G following the manufacturer’s instructions of Lipofectamine 3000 (Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The eluted RNA was combined with Applied Biosystems™ TaqMan™ Fast Virus 1-step master mix (ThermoFisher, Waltham, MA, USA). The target regions for the CSG ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of the RNA extraction final elution was reverse-transcribed to cDNA and amplified according to the manufacturer’s protocol using TaqMan™ Fast Virus 1-Step Master Mix (ThermoFisher Scientific). The primer pair was as follows ...
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bioRxiv - Cell Biology 2021Quote: ... Induced pluripotent stem cells were derived from human fibroblasts using Sendai virus-based reprogramming kit (CytoTune-iPS Sendai Reprogramming Kit; cat #. A13780-01; Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant containing the virus was then used fresh or stored by snap-freezing aliquots supplemented with 8 μg/ml polybrene (Fisher Scientific) in ethanol/dry ice and stored at -80° C ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from 50 μl of virus inoculum suspension using the PureLink Viral RNA/DNA Mini kit (Themo Fisher Scientific), as per manufacturer’s directions.
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bioRxiv - Microbiology 2022Quote: ... and resuspended in thawed Geltrex lactose dehydrogenase elevating virus free (LDEV-free) reduced growth factor basement membrane matrix (Thermo Fisher Scientific). Three 10-μl drops of the matrix-cell suspension were pipetted into wells of a Nunclon Delta 24-well plate (Thermo Fisher Scientific ...