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Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: Cells in all three types of constructs were labelled with TO-PRO™-3 (Invitrogen™, ThermoFisher Scientific) for the nuclei ...
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bioRxiv - Genetics 2022Quote: Calu-3 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM)/F12 (1:1) GlutaMAX™ (ThermoFisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: HCT116 cells were cultured in a 6-well plate at 300,000 cells per well in 3 ml McCoy’s 5A medium (Gibco) supplemented with 10% heat inactivated FBS and with 90 UI/mL Penicillin ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then again washed 3 x 15 min in WB at RT with DAPI (Thermo Scientific #62248) diluted to 1 ug/ml in the final WT wash ...
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bioRxiv - Pathology 2019Quote: Mouse inner medullary collecting duct (mIMCD-3) cells were grown in 50% DMEM high-glucose pyruvate (Gibco, 41966052), 50% F-12 Nutrient Mixture (Gibco ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were first rinsed 3 times in 1X PBS Ca+ and Mg+ free (Thermo Scientific, Cat. No. 14190144), then dissociated in Accutase (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... JEG-3 and DU145 cells were cultured in 10% FBS in MEM + Earle’s salts and L-Glutamine (Gibco). PC-3 cells were cultured in 10% FBS in F-12K Kaighn’s modification + L-Glutamine (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: ... The cDNA was synthesized from total RNA of HT29 cells using Super Script 3 First-Strand Synthesis (Invitrogen). Quantitative PCR amplification was performed using a C1000 Touch Thermal Cycler ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were freeze/thawed 3 times and further lysed with RIPA (radioimmunoprecipitation) lysis buffer (Thermo Fisher Scientific, # 89901) supplemented with protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed 3 times with PBS and mounted on slides using ProLong Diamond antifade mountant (Thermo Fisher). For live-cell imaging ...
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bioRxiv - Microbiology 2020Quote: 40000 Calu-3 cells (50µl/well) were seeded in 96-well white optical-bottom tissue culture plates (Nunc). 48h post-seeding ...
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bioRxiv - Microbiology 2021Quote: ... Caco2 colorectal adenocarcinoma and Calu-3 lung adenocarcinoma cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM; from Gibco) with Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... counted and 1×105 viable cells were used to produce cytospin slides (Thermo Shandon Cytospin 3, Thermo Scientific). Cytospin slides were fixed in 4% PFA ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were washed 3 times with PBS and coverslips were mounted with Vectashield Antifade Reagent containing DAPI (ThermoFisher).
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bioRxiv - Molecular Biology 2019Quote: ... Cells were passaged every 3-4 days with 0.25% trypsin-EDTA solution (Life Technologies, cat. no. 25200-056) and washed with sterile PBS (Life Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... cells were washed with 3% BSA in PBS three times and incubated with appropriate secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and Human lung adenocarcinoma epithelial Calu-3 cells (ATCC) were maintained in a high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 minutes prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Genomics 2020Quote: ... pVSV-G and pLJM1-3×Flag-BUD13-6HIS into HEK293T cells using Lipofectamine 3000 (Thermo Fisher Scientific, L3000015). Lentiviruses were collected from conditioned media after 48 h co-transfection and filtered through 0.2μm syringe filter ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 x 106 WT HEK293T cells were plated in 10 cm plates and transfected using Lipofectamine 2000 (Invitrogen) with the following plasmids expressing V5-SBP fusions ...
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bioRxiv - Immunology 2021Quote: B cells (3×106) were pelleted by centrifugation and resuspended in 0.3 ml of Trizol (Thermo Fisher Scientific) and RNA spike-in (generated by in vitro transcription ...
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bioRxiv - Immunology 2020Quote: ... 5% CO2 and medium were changed every 3 days and cells were passaged with 0,05% trypsin-EDTA (Invitrogen) twice per week.
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bioRxiv - Microbiology 2022Quote: ... Vero-E6 cells and Calu-3 were treated with 5 μg/ml or 1 μg/ml puromycin (Gibco), respectively ...
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bioRxiv - Immunology 2022Quote: Calcium imaging was performed on EROS-/- and control cells using the Rhod-3-AM calcium imaging kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed with flow buffer and resuspended in flow buffer containing TO-PRO-3 (Invitrogen, T3605). TO-PRO-3low/CD45-/GFP+ cells were sorted on a BD FACSAria II ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed twice with PBS and loaded with 3 µM Fura-2 AM (Thermo Fisher, Waltham, MA) diluted in a modified Krebs-Ringer buffer solution [KRBH ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were then stained with secondary antibodies diluted 1:500 in 3% BSA and DAPI (Invitrogen D3571) for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Non-adhered cells were removed by washing 3-times with warm PBS (Gibco, DPBS with Mg2+ and Ca2+), and adhered cells were exposed to exNef or exCont (1 μg/ml protein ...
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bioRxiv - Microbiology 2022Quote: ... BHK-21 cells were maintained in minimum Essential Medium (MEM) REGA-3 (Thermo Fisher Scientific, Waltham, MA, USA) and all other cell lines in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... The MUTZ-3 cell line (DSMZ, no ACC 295) was cultured in Minimum Essential Medium Alpha (MEMα) (Gibco, Thermo Fischer Scientific Cat# 22571020 ...
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bioRxiv - Developmental Biology 2023Quote: ... 3 μg of the plasmids was transfected into the 393T cells using Lipofectamine 2000 (Thermo Fisher Scientific #11668027) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Calu-3 (ATCC HTB-55) and Caco2 (ATCC HTB-37) cells were cultured in DMEM (Gibco 41966-029) containing 1% Penicillin-Streptomycin (PS ...
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bioRxiv - Genomics 2024Quote: ... the entry vectors containing the cloned 3’ UTR regions were transformed into TOP10 competent cells (Thermo Fisher Scientific) and plated on LB agar plates containing 20 mg/µL of kanamycin ...
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bioRxiv - Microbiology 2024Quote: ... exponentially growing cells in suspension were diluted to 3×109 and 5 µl spotted on 1.5% agar (Gibco) supplemented with 0.5% CTT and immediately covered with a coverslide ...
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bioRxiv - Biophysics 2023Quote: ... This was done by dissociating cells for 3 min at 37°C using TrypLE Express (ThermoFisher, Cat#: 12605010), neutralizing with 1:10 volumes (CDM) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Medium was exchanged every 3 days and cells were regularly passaged before reaching ∼80% confluency using TrypLE (Gibco) for dissociation ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were washed 3 times in PBS and mounted with Prolong mounting medium with DAPI (Molecular probes).
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bioRxiv - Microbiology 2023Quote: ... the cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions for kinetic assays and fluorescence in the live cells ...
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bioRxiv - Cell Biology 2023Quote: ... After 24 h the cells were washed 3 times with prewarmed Hank’s Balanced Salt Solution (HBSS, Thermo Fisher) and stained with 1 μM of Bodipy red ER-tracker™ (Thermo Fisher E34250) ...
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bioRxiv - Bioengineering 2023Quote: Calu-3 and HLFC viability were assessed using the LIVE/DEAD™ cell imaging kit (Invitrogen™, R37601). All fluorescent images were acquired using an EVOS™ M7000 microscope (Invitrogen™ ...
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bioRxiv - Genetics 2023Quote: ... 3-5 million cells or approximately 15 ug of DNA crosslinked with 2% PFA (Fisher Scientific F79-500) were used as input per reaction ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 min prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Microbiology 2023Quote: ... of confluent MDCK cell monolayers overlaid with 3 ml of Leibovitz’s L-15 medium with L-glutamine (Gibco), 0.2 mM HEPES buffer ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 3 μg mRNA at a mass ratio of 1:1 using Lipofectamine MessengerMAX (Invitrogen) according to the manufacturer’s guide ...
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bioRxiv - Cancer Biology 2023Quote: Cell proliferation was assayed by reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Invitrogen, M6494). MTT was freshly dissolved into PBS at a stock concentration of 12 mM and diluted into phenol red-free DMEM with 10% FBS for a final MTT concentration of 2 mM ...
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bioRxiv - Cell Biology 2023Quote: ... cells washed 3 times with PBS and 5ml of serum-free DMEM deficient in lysine and arginine (Gibco) with (13C6)-labelled L-lysine (CK Isotopes Ltd ...
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bioRxiv - Cell Biology 2022Quote: Live/Dead™ Cell Imaging Kit and CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) were used for detection of cell apoptosis based on the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS and incubated with an Alexa-568 conjugated secondary antibodies (ThermoFisher Scientific) for 1 hour at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei count and lysis optimalization was determined with diluted Trypan blue solution with Countess 3 cell counter (Invitrogen). Each sample was loaded with 10 000 nuclei and library preparation performed according to the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression User guide CG000338 Rev E and libraries constructed using the 10X Genomics Chromium technology ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 × 108 asynchronised TIG-3-20 cells (per time point) were labeled with 20 μM EdU (Thermo Fisher) for 11 min ...