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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then transduced with the lentivirus for 3 days before selection with 1μg/ml puromycin (Thermo Fisher Scientific). shRNA target sequences ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed 3 times in PBS and slides were then mounted in ProLong Gold Antifade + DAPI (ThermoFisher, #P36935). Slides were cured overnight at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Fixed cells were washed with 1X DPBS(-) once and permeabilized and blocked in a 3% BSA solution (Thermo Fisher) with 0.5% Triton-X-100 (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... counted and total RNA was recovered from 3×106 cells using 1 ml of Trizol (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... counted and total RNA was recovered from 3×106 cells using 1 ml of Trizol (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... The next day we transfected the cells with 7.5μl 10μM siRNA pools (3 siRNAs against same gene) using 100μl OPTIMEM (Gibco, prod. number 31985070) and 7.5μl Lipofectamine® RNAiMAX Transfection Reagent (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: 70 % of confluence C2C12 cells were transfected with 3 μg GFP lamin mutants by lipofection (Lipofectamine 3000, Life Technologies) following manufacturer instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were dissociated on day 3 with room temperature StemPro™ Accutase™ Cell Dissociation Reagent (Thermo Fisher, A1110501) and re-plated into PDL and Laminin (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s protocol and visualized using FITC 488 nm filter ...
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bioRxiv - Neuroscience 2019Quote: ... Neuroepithelial cells were generated by culturing for 3 weeks in neural induction media containing 1x N2 supplement (Life Technologies) and 2 μg/mL heparin (Sigma Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... 20.25 μg 3×Flag-tagged dCas9 plasmid and 20.25 μg sgRNA plasmid were transfected into cells using Lipofectamine 3000 (Invitrogen). Two biological replicates were performed for each condition ...
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bioRxiv - Microbiology 2019Quote: ... was generated by RT-PCR amplification of the 3’ UTR region from ZIKV-infected cells and the resulting PCR product cloned into pCR-TOPO2.1 (Invitrogen). The actin and ZIKV probes were randomly labeled with α32P dATP (Perkin Elmer ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 mL of the DMEM media was removed and ID8 cells were co-transfected with Lipofectamine 3000 (L3000015 Invitrogen) 35 µL of Plus reagent / 41 µL of Lipofectamine 3000 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then fixed in 3.7% formaldehyde and nuclear counterstaining was performed with TO-PRO-3 Iodide (Life Technologies) for 5 min ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were loaded with culture medium containing 3 μM of the fluorescent calcium indicator Fluo-4/AM (Life Technologies) and Pluronic F-127 (0.2% w/v ...
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bioRxiv - Bioengineering 2021Quote: ... Medium was changed every 2-3 days and cell passages were carried out using a TrypLE Express solution (Gibco). Human TERT-immortalized gingival fibroblasts (hTERT-HGF ...
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bioRxiv - Biochemistry 2020Quote: ... were seeded at 0.3 x 106 cells/mL in 3 L (total working volume) of CD-CHO medium (Invitrogen) supplemented with 50 µg/L rMSB ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Cells were then washed twice with 3% BSA in DPBS and incubated with Anti-Strep PE-Cy7 (Thermofisher SA1012) at 5 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... 2 and 3 (100 pmol for each) or control siRNA were transfected into HeLa cells using Lipofectamine 2000 (Invitrogen). 48 h after transfection ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... seeded with 2,000 cells/well and stained with Hoescht 33258 (1µg/mL) and CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at a dilution of 1000x ...
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bioRxiv - Microbiology 2021Quote: ... A total of 20 μg of whole cell lysate were resolved on a 3-8% Tris-Acetate NuPAGE (Invitrogen) and analyzed by immunoblotting with anti-SPTBN1 ...
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bioRxiv - Microbiology 2021Quote: ... discoideum cells were counted and plated at different dilutions (from 1.3×105 to 3×104 cells/well) in a white F96 MicroWell plate (Nunc) covered with a gas permeable moisture barrier seal (Bioconcept) ...
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bioRxiv - Microbiology 2021Quote: ... The other half of the wells of the Caco-2 cells was subsequently incubated anaerobically for 3 h with 0.3% gentamicin (50 μg/ml, Gibco) to eliminate all extracellular L ...
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bioRxiv - Microbiology 2021Quote: ... The other half of the wells of the Caco-2 cells was subsequently incubated anaerobically for 3 h with 0.3% gentamicin (50 µg/ml, Gibco) to eliminate all extracellular L ...
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bioRxiv - Cancer Biology 2020Quote: A number of 150.000 MDA-MB-468 cells were plated in 3 ml of DMEM culture medium (Life Technologies) in 6-well plates (Greiner Bio-One) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with lentiviral supernatant and 3 days later selection begun with 6 μg/mL puromycin (Thermo Fisher), which was maintained during all subsequent culturing.
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™ ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 × 103 Panc-1 cells per 30 µl were pre-labeled with Hoechst 33342 (Thermo Fisher Scientific; Waltham, MA) for 1 hour prior to trypsinization and resuspended in tumor sphere-forming media (Irvine Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were harvested at 500 x g for 3 min and washed twice with Hank’s Balanced Salt Solution (Gibco™ HBSS ...
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bioRxiv - Immunology 2022Quote: ... Plat-E cells at 0.3*106 cells/well of 12-well plate were seeded and cultured overnight before transfection with 100μl OptiMEM (Gibco), 3μl TransIT-293 ...
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bioRxiv - Genomics 2022Quote: ... The cells were then transfected with 3 μg of the plasmid constructs using 4.5 μL of Lipofectamine 2000 (Thermofisher), as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Dissociated cells were centrifuged at 800 rpm for 3 minutes and resuspended in RPMI-1640 media (ThermoFisher Scientific (Gibco); 11875093 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell viability was determined by the MTT [3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Invitrogen, Waltham, MA) reduction assay ...
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bioRxiv - Molecular Biology 2020Quote: The rat insulinoma cells (INS-1, 832/3)54 were cultured in RPMI-1640 medium (Thermo Fisher Scientific, 11875119) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... siERH targeting 3′ UTR of ERH was transfected into 50% confluent HEK293E cells using lipofectamine 3000 (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... cells were cultured for 3 days in induction media (IM) consisting of Knockout DMEM/F12 (Thermo Scientific Cat. #12660012), 1X N-2 Supplement (Thermo Scientific Cat ...
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bioRxiv - Microbiology 2020Quote: ... Lentiviruses were used to transduce 293T-ACE2 cells (MOI = 3) pre-treated with 10 μg/ml Polybrene (Life Technologies), followed by selection of resistant cells with Blasticidin (InvivoGen ...
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bioRxiv - Neuroscience 2021Quote: ... The culture medium was changed every 2 to 3 days and the cells were split every 5 to 7 days using 0.25% Trypsin-EDTA (Gibco), up to 20 times ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were marked with 2,3×10−3 μg/μL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 minutes at room temperature in the dark ...
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bioRxiv - Microbiology 2021Quote: Human adenocarcinomic lung epithelial (Calu-3) cells (ATCC, HTB-55) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Systems Biology 2022Quote: Vero E6 and Calu-3 cells were maintained in standard medium (Minimum Essential Medium (MEM; catalogue number 11095; Gibco) supplemented with 9% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Red blood cells were removed by treatment with 3-5 mL of ACK lysis buffer (Gibco, Cat. No. A1049201) and a subsequent washing step in RPMI 1640 with 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... Dissociated cells were centrifuged at 800 rpm for 3 minutes and resuspended in RPMI-1640 media (ThermoFisher Scientific (Gibco); 11875093 ...
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bioRxiv - Physiology 2022Quote: ... The nucleic acids present in the pituitary gland cells were visualised with TOTO-3 (1:2000, Thermo Fisher Scientific). Sections were incubated with TOTO-3 for 15 min on an agitated surface ...
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bioRxiv - Neuroscience 2019Quote: The HEK293T cells were seeded at a density of 3 × 104 cells/per 48 wells and transfected according to the manufacturer’s protocol (Lipofectamine 2000, Invitrogen). The Renilla luciferase-containing reporter (pRL-TK Vector ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then permeabilized with 0.1% Triton X-100 and stained with TO-PRO-3 (T3605, Thermo Fisher Scientific) and rhodamine phalloidin (Molecular Probes) ...
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bioRxiv - Immunology 2020Quote: ... 1×106 CAR T cells were co-cultured with γ-irradiated K562-CD19 or K562-CD19-PD-L1 at 1:3 effector:target (E:T) ratio for 7 days and counted with the countess II Automated Cell Counter (ThermoFisher, USA).
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bioRxiv - Microbiology 2021Quote: ... After 3 days the overlay was removed and cell monolayers were fixed with 4% formaldehyde (28906, Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Biophysics 2021Quote: Vero-E6 (ATCC, CRL-1586) and Calu-3 cells (ATCC HTB-55) were cultured in high glucose DMEM (Gibco) supplemented with 10% FBS (R&D Systems) ...