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Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 7 µl total RNA was amplified in a mix containing 5 µl of TaqMan Fast Virus 1-step master mix (Life Technologies), 0.4 µl primer-probe mix for IAV 40 and 9.6 µl distilled water ...
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bioRxiv - Microbiology 2022Quote: Copies of the SARS-CoV-2 N gene (genomic) were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... The virus-containing samples (50 μl) were serially diluted in 450 μl aliquots of serum-free Opti-Pro medium (Gibco, UK), after which 200 μl from each dilution were added in duplicates to the cells ...
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bioRxiv - Microbiology 2022Quote: ... 5 µl total RNA was amplified in a mix containing 5 µl of TaqMan Fast Virus 1-step master mix (Life Technologies), 1 µl primer-probe mix for β-actin ...
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bioRxiv - Microbiology 2023Quote: ... The samples were used for quantitating HuNoV RNA GEs by means of reverse transcription-quantitative PCR (RT-qPCR) analysis using a TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and GII specific primer/probe sets ...
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bioRxiv - Cancer Biology 2024Quote: ... Subsequent qPCR was performed using the TaqMan Fast Virus 1-Step Master Mix and StepOnePlus Real-Time PCR System (Thermo Fisher). The primers purchased from TaqMan were ...
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bioRxiv - Neuroscience 2024Quote: ... HEK cells below passage 10 are plated into a 15 cm dish/virus (Fisher 08-772-24) in DMEM-H (DMEM-h (invitrogen 10569010) + NEAA (Invitrogen 11140050) + 10% FBS (invitrogen 16140071 ...
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bioRxiv - Genetics 2024Quote: The derivation of iPSCs was carried out by the NHLBI iPSCs core facility using the Cytotune 2.0 Sendai virus kit (ThermoFisher Scientific, A16517) with a few modifications for high throughput reprogramming essentially as described earlier (19) ...
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Anesthetized mice were inoculated intranasally with 30 μL of virus diluted in chilled 1X PBS (MHV) or administered undiluted in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Both assays were performed using the Taqman Fast Virus 1-Step Master Mix RT-qPCR kit (Applied Biosystems Inc., Carlsbad, CA) on an LC96 instrument (Roche ...
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bioRxiv - Cell Biology 2023Quote: The adeno-associated virus MyoAAV.2A was produced using the AAV-MAX Helper-Free AAV Production System Kit (Thermo Fisher Scientific). At 72 h post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were infected (multiplicity of infection of 6 for each virus) with separate baculoviruses (prepared using the Bac-to-Bac Baculovirus Expression System, Invitrogen) containing the CXCL12LRHQ gene as well as the ACKR3 gene (residues 2-362 ...
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bioRxiv - Immunology 2023Quote: ... 2μg liver RNA per sample was used for qRT-PCR analysis for Plasmodium 18S rRNA using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). Data were normalized for input to the GAPDH control for each sample and are presented as ratios of Plasmodium 18s rRNA to GAPDH RNA signals ...
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bioRxiv - Microbiology 2023Quote: We diluted freshly purified virus samples in DPBS and combined three volumes of the virus with one volume of NuPAGE LDS Sample Buffer (4×; Thermo Fisher) containing 100 mM dithiothreitol ...
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bioRxiv - Microbiology 2023Quote: ... For intravenous inoculation mice were restrained and injected via tail vein injection with 100 to 200 μl of virus or control inoculum using a 29G insulin syringe (Fisher Scientific). For natural transmission ...
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bioRxiv - Pathology 2023Quote: ... was reverse transcribed at 37°C for 50 min in a 20 µl reaction mix containing 200U Moloney murine leukemia virus (MMLV) reverse transcriptase (Life Technologies), 100 ng random primers ...
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bioRxiv - Microbiology 2023Quote: ... Viral genome levels were quantified by qPCR using virus specific primer probe sets (described above) and normalised to GAPDH using an Applied Biosystems FAM/MGB probe mix (Thermofisher, 4333764F).
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bioRxiv - Neuroscience 2024Quote: ... The MS19-ES-H line was reprogrammed from PBMCs from a healthy female donor with a Cytotune Sendai Virus kit (Thermo Fisher).
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... and (MtWOX5) 5’-CACCATGCAGACGGTCCGAGATCTGTC-3’ and 5’- CCTTCGCTTAAGTTTCATGTAA-3’ (AhWOX5) and cloned into pENTR-dTOPO (Invitrogen). The entry clones of AhWOX5 & MtWOX5 recombined through LR clonase Gateway technology (Invitrogen ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... The eight rice 14-3-3 genes cloned in the yeast two-hybrid vector pDEST22 (Invitrogen) were used from a previous study (Deb et al. ...
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bioRxiv - Cell Biology 2021Quote: ... N-hydroxysulfosuccinimide (sulfo-NHS) and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) from Thermo Scientific were dissolved in 18 MOhm DDW immediately before use ...
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bioRxiv - Bioengineering 2022Quote: ... 1-ethyl-3-(−3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was purchased from Fisher Scientific (Pittsburgh, PA, USA). 2-Bromo-2-methylpropionic acid (BMPA) ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 24 minutes in a mixture of 3% 3-Aminopropyltriethoxysilane (ACROS Organics, 430941000) and 5% acetic acid in HPLC EtOH ...
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bioRxiv - Molecular Biology 2019Quote: ... the PCR products obtained with 2 primers (AV48_SMCHD1_gPCR_F: 5’-AGGAGCGCGTTTGAATCGG-3’, AV47_SMCHD1_gPCR_R 5’-CTTCGCGTACCTGACACACAC-3’) were TOPO-cloned (Thermo Fisher, #450071) and sent for sequencing.
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bioRxiv - Immunology 2020Quote: ... 3 μL RT reaction mix consisting of 3 μL of 50 μM random hexamers (Thermo Fisher), 0.8 μL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Molecular Biology 2022Quote: ... siM1-4: 5’ -ACTGGTAGCTTATTAAAGATT- 3’) and the HOXA1 siRNA (5’ - AGAACTTCAGTGCGCCTTATT- 3’) were purchased from Invitrogen™ (Silencer® Select siRNA ...
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bioRxiv - Cancer Biology 2024Quote: Total mRNA from 3 siCT and 3 siPAK2 independent experiments was extracted using TRIZOL reagent (Invitrogen) according to recommended procedures ...
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bioRxiv - Microbiology 2024Quote: ... This was followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for about 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated for 24 min in a mixture of 3% 3-aminopropyltriethoxysilane (ACROS Organics, 430941000) and 5% acetic acid in HPLC ethanol ...
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bioRxiv - Immunology 2023Quote: ... To assess active caspase 3 activity the CaspGLOW™ Fluorescein Active Caspase-3 Staining Kit (Invitrogen) was used ...
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bioRxiv - Cell Biology 2023Quote: ... Target mtDNA gene (Forward primer: 5′-CACCCAAGAACAGGGTTTGT-3′, Reverse primer: 5′-TGGCCATGGGTATGTTGTTAA-3′, Invitrogen custom primers) and reference 18S ribosomal RNA gene (Forward primer ...
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bioRxiv - Neuroscience 2024Quote: Rabbit monoclonal or polyclonal antibodies include those against 14-3-3 (Invitrogen, 51-0700, 1:1000); clathrin heavy chain (Abcam ...
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bioRxiv - Genetics 2021Quote: ... Cas9 protein (Invitrogen), a single-stranded 100-bp oligo donor nucleotide (ssODN ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein gel (Invitrogen). Antibodies were added according to the manufacturers recommended conditions ...
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bioRxiv - Neuroscience 2022Quote: ... a cell surface biotinylation assay was performed using a commercially available kit (Pierce Cell Surface Protein Isolation Kit, Thermo Fisher Scientific, catalog #89881). Eluates were subjected to immunoblot analysis using a Plexin-A4 antibody (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: The surface proteins of HEK293 and hippocampal cells were biotin-labeled and isolated using a Pierce cell surface protein biotinylation and isolation kit (A44390, Thermo Scientific, IL, USA). The protein samples were resolved using 10% SDS PAGE and transferred to polyvinylidene fluoride membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were washed twice in PBS and the cellular contents from the dry cell pellets was fractionated using the subcellular protein fractionation kit for cultured cells (ThermoFisher Scientific, Cat# 78840) exactly according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 uM FluoZin-3 AM (Invitrogen) was add to dishes to stain cells for 1 hour and then was washed twice with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 3 washes of 5X SSCT (ThermoFisher Scientific 15557044 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-phosphohistone 3 (1:100, Invitrogen), anti-phosphoAMPK (1:200 ...
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bioRxiv - Immunology 2021Quote: ... 3 mM MgCl2 (Thermo Fisher AM9530G), 20 mM Tris-HCl pH 7.5 (Thermo Fisher ...
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bioRxiv - Biophysics 2019Quote: ... or 3 M NaCl (Fisher Scientific), in 25 mM ammonium bicarbonate buffer containing 25 mM dithiothretol (DTT ...
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bioRxiv - Neuroscience 2019Quote: ... IL-3 (20 ng/mL, ThermoFisher), IL-6 (20 ng/mL ...
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bioRxiv - Neuroscience 2019Quote: ... FLT-3 (100 ng/mL, ThermoFisher), IL-3 (20 ng/mL ...
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bioRxiv - Microbiology 2019Quote: ... 3 um particle size (Thermo Scientific) and separated into a Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Neuroscience 2020Quote: ... TO-PRO-3 (Thermo Fisher Scientific), or Hoechst 33258 in the blocking buffer for 60 min.
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bioRxiv - Neuroscience 2020Quote: ... 3 µl of Liptoectamine RNAimax (Invitrogen) was diluted in 150 µl RPMI media and mixed with 150 pmol of siRNA in 150 µl RPMI media and left for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 3% fetal bovine serum (Life Technologies) and 1% Penicillin/Streptomycin/L-glutamine (Life Technologies) ...