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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... cells were marked with 2,3x10-3 µg/µL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 min at RT in the dark ...
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bioRxiv - Genomics 2024Quote: ... Fresh medium was added every 2-3 days and cells were passaged using 1X TrypLE Express (Life Technologies).
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bioRxiv - Molecular Biology 2023Quote: Total RNA from 3 x 108 cells at the exponential growth phase was isolated using TRIzol reagent (Invitrogen). RNase-free DNase I treatment (Thermo Scientific™ ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting plasmid was transfected into HEK293 cells at 70% confluency using Genejuice (Fisher Scientific 70-967-3) at a 1:3 ratio (DNA ...
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bioRxiv - Immunology 2024Quote: ... and stained with intracellular cytokine antibodies (Supplementary Table 3) LIVE/DEAD fixable cell staining kit (Thermofisher Scientific, USA) was used to exclude dead cells from analysis.
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bioRxiv - Molecular Biology 2024Quote: ... Knockdown of the protein was monitored by western blotting against the V5×3 epitope using α-V5 mouse primary antibody (Invitrogen / Thermo Fisher Scientific – 1:1000). Oligonucleotides used for PCR amplification are given in Table S4.
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Cell Biology 2021Quote: ... Total proteins were quantitated using Qubit Protein Assays (ThermoFisher) and equal loads of total protein were separated on a NuPAGE 4-12% Bis-Tris mini gel (NP0336BOX ...
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bioRxiv - Neuroscience 2022Quote: ... BCA protein assay kit (ThermoFisher Pierce BCA Protein Assay) was used to determine the protein concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was quantified by BCA Protein Assay (Thermo Scientific) and 50 µg of total protein in 100 mM TEAB and 10% acetonitrile was incubated at 99°C for 1 h.
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bioRxiv - Genetics 2020Quote: ... protein quantification using BCA Protein Assay Kit (Thermo Fisher) was done ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were immunoprecipitated with Protein A Dynabeads (ThermoFisher) conjugated to the relevant antibody (anti-MqsRA ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified using BSA Protein Assay Kit (ThermoFisher). Equal amounts of protein were boiled with 6X loading buffer (375mM Tris HCL ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was measured using BCA Protein Assay (ThermoFisher). For anti-NP immunoprecipitation ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined with Bradford protein assay (Thermofisher), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cas9 protein (Fisher Scientific A36498 Truecut Cas9 Protein v2) and gRNA were co-electroporated into G1 ES cells derived from an F1 hybrid blastocyst of 129S6 x C57BL/6J ...
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Allelic diversity uncovers protein domains contributing to the emergence of antimicrobial resistancebioRxiv - Microbiology 2022Quote: ... alongside protein standards (SeeBlue Pre-stained Protein Standard, Invitrogen). Gels were run at room temperature for 150 min at 80 V in running buffer ...
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bioRxiv - Plant Biology 2022Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4°C for 2 hours ...
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bioRxiv - Plant Biology 2023Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and mixture was rotated at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... cas9 protein (Fisher Scientific A36499 TRUECUT CAS9 PROTEIN V2) and the gRNA with concentrations of 10 ug ...
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bioRxiv - Biochemistry 2023Quote: ... protein quantified by BCA protein assay ( Thermo scientific #23250), and 30 μg of the proteins were loaded on 4-12% Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was determined by Qubit protein assay (Invitrogen) in a Qubit 4 fluorometer (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... 200 μL of Protein A (Dynabeads Protein A, Invitrogen) beads were washed twice in coIP buffer containing PIs ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were quantified using Coomassie Protein Assay Reagent (ThermoFisher). Protein samples (30 μg ...
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bioRxiv - Microbiology 2020Quote: ... five-fold serial dilutions of each virus stocks were prepared in a separate 96-well plate in Opti-MEM (Gibco). Medium in the 96-well plate was removed and the cells were washed with 100 µL of warm PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and quantitative PCR (RT-qPCR) was performed using 0.25 µl of lysate and Taqman Fast Virus 1-step Master Mix (Applied Biosystems) on a CFX384 thermal cycler (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μl of RNAse-Out and 1 μl of the Moloney Murine Leukemia Virus reverse transcriptase enzyme (MML-V, Invitrogen). Quantitative PCR was carried out in a total of 15μl consisting of 3μl cDNA ...
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bioRxiv - Plant Biology 2020Quote: ... NAA50 was cloned into the cauliflower mosaic virus 35S promoter vector pEarleyGate100 (Earley et al. 2006) using a modified multisite Gateway recombination cloning system (Invitrogen) as described in (Qi et al ...
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bioRxiv - Developmental Biology 2019Quote: Pure cultures of 10 × 104 prostate stromal cells seeded on twelve well plates were transduced using Cytotune 2.0 Sendai Virus reprogramming vectors (KOS, c-Myc and Klf4, Life Technologies) at a multiplicity of infection of 5-5-3 (KOS MOI=5 ...
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bioRxiv - Microbiology 2019Quote: ... 0.35μg of each of 10 reovirus genes and 0.8μg C3P3 (T7 RNA Polymerase linked with (G4S)4 to NP8686 African Swine Fever Virus capping enzyme) were diluted in 50μl Opti-MEM (31985070, ThermoFisher Scientific). 11μl TransIT®-LT1 (MIR2300 ...
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bioRxiv - Cell Biology 2019Quote: ... and HY03 were reprogrammed using the Sendai virus and the CytoTune®-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific), and display all the PSC features ...
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bioRxiv - Biochemistry 2020Quote: ... The plasmid was linearized with XbaI and in vitro transcribed into the genomic RNA of DENV-Luc virus using the T7 Megascript Kit (Ambion) in the presence of m7G(5’)ppp(5’)G RNA Cap Structure Analog (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... The virus particles were then harvested 24 to 48 hours later by passing through a 0.45 µm syringe filter (Thermo Scientific). Viruses were mixed with an equal volume of fresh media supplemented with 10% FBS and polybrene was added at a final concentration of 5 µg/mL to increase infection efficiency ...
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bioRxiv - Cell Biology 2021Quote: ... by the acid guanidinium/phenol/chloroform method using ISOGEN (Nippon Gene) was converted to cDNA using Moloney murine leukemia virus reverse transcription (Invitrogen) and oligo-dT primers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... ROMT2 and ROMT3 were transferred into the binary destination vector pB2GW7 including Cauliflower mosaic virus (CaMV) 35S promoter through Gateway LR clonase recombination (Invitrogen). Together with ROMT1 and STS48 constructs (Schmidlin et al. ...
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bioRxiv - Microbiology 2021Quote: ... 1μL of each extracted RNA was used to performed one step RT-qPCR using TaqMan Fast Virus 1-Step Master Mix (Invitrogen). The relative quantities of envelope (E ...
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bioRxiv - Microbiology 2021Quote: Nucleic acids were extracted from purified virus samples either using the PureLink Viral RNA/DNA Mini Kit (Thermo Fisher Scientific) or by phenol/ether method combined with ethanol precipitation ...
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bioRxiv - Microbiology 2021Quote: ... Five μl RNA (150 µl elution volume) was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using 100ng total RNA compared to an RNA standard curve using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on a Quant Studio 3 (Applied Biosystems).
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan(tm) Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were based on (21 ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified by RT-qPCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Primers and probes were used as described previously (45) ...
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bioRxiv - Immunology 2022Quote: ... were pulsed for 1 hr with 10μg/ml influenza virus NP(366-374) peptide and were subsequently labeled for 10 min at 37°C with 5 μM CFSE (Invitrogen) (CFSEhigh ...
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bioRxiv - Immunology 2022Quote: ... The extracted RNA was then added to TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Waltham, MA) containing primers and a fluorescently labeled hydrolysis probe specific for mRNA from the nucleocapsid gene of SARS-CoV-2 (forward primer 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was detected with the CDC assay kit (IDT) and the TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) on the QuantStudio3 System (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... The nCoV_IP2 primer-probe set was used in combination with TaqMan fast virus 1-step master mix (Thermo Fisher Scientific) to detect viral RNA ...
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bioRxiv - Cell Biology 2022Quote: ... Assays were performed in 24-well plates coated with Geltrex LDEV (lactose dehydrogenase elevating virus)-Free Reduced Growth Factor Basement Membrane Matrix (ThermoFisher) at 75 µl/cm2 according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... The resuspended virus was purified using a 30% (wt/vol) sucrose cushion at 100,000 × g (Sorvall Discovery 90SE, Thermo Fisher) for two- and-a-half hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 500 ng of RNA was reversely transcribed using random primers (Eurofins) and Moloney murine leukaemia virus reverse transcriptase (M-MLV, Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Heathy human donor PBMCs or skin fibroblasts were reprogrammed into iPSCs using the integration-free based Sendai virus (Cytotune 2.0 kit from Life Technologies). Low passage primary fibroblasts were used to increase reprogramming efficiency ...