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Citations for Thermo Fisher :
1 - 50 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... aliquoted in volumes of ∼700 μl in Eppendorf tubes and sonicated on ice until the DNA fragments were enriched to around 200–500 bp with a manual sonicator (model 100, Fisher Scientific). For ChIP assays ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP (Invitrogen, ref A10262, dilution 1:200), anti-TUJ1 (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... and Alexa Fluor 647 donkey anti-rabbit IgG (Invitrogen, ref A31573). Nuclear staining was performed using 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were incubated with the Alexa Fluor 488-or 555-conjugated secondary antibodies (1:500 in 1% BSA, Thermo Fisher) for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 647 donkey anti-mouse IgG (Invitrogen, ref A21448), Alexa Fluor 647 donkey anti-goat IgG (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 647 donkey anti-goat IgG (Invitrogen, A21447), and Alexa Fluor 647 donkey anti-rabbit IgG (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: The siRNA (GenePharma) used to knock down the expression of USP8 was transfected into cells by using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... GFP (14-6674-82, 1:1,000, Invitrogen), EEA1 (610456 ...
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bioRxiv - Cell Biology 2024Quote: ... containing a protease inhibitor cocktail (Thermo Scientific), and then pelleted at 12,000 g for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... donkey anti-rabbit (Alexa Fluor 555, Invitrogen, ref A31572) and donkey anti-rabbit (Alexa Fluor 647)(Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections were performed with Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... BS3 cross-linker (Thermo Scientific, 21580) was diluted to 5 mM with 1X sterile PBS ...
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bioRxiv - Cell Biology 2024Quote: Dynabeads Protein A for Immunoprecipitation (ThermoFisher, 10001D) were resuspended in PBS-Tween (0.2%)+ Protease Inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... Non-crosslinked antibody was removed with 2 washes of Pierce Gentle Antibody elution buffer (Thermo Scientific, 21013), and the beads washed twice more with 1X RIPA ...
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bioRxiv - Cell Biology 2024Quote: ... Subsequently they were mounted with Prolong Diamond Antifade Mountant with DAPI (ThermoFisher Scientific, P36962) and promptly imaged using Zeiss Axio Imager M2 Plus Wide Field Fluorescence Microscope.
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bioRxiv - Cell Biology 2024Quote: ... Cells were preserved in ProLong Diamond anti-fade mounting medium (Thermo Scientific, P36965).
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bioRxiv - Cell Biology 2024Quote: ... Sections were applied with Prolong Gold Antifade reagent with DAPI (P36935, Thermo Fisher Scientific, USA) and covered with cover glass before proceeding with imaging ...
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bioRxiv - Cell Biology 2024Quote: ... 50µl of urine was centrifuged to collect cells that were then incubated with 10μM dihydroethidium (ThermoFIsher Scientific, D23107) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 mM L-Glutamine (Gibco, 25030-081) in Dulbecco’s Modified Eagle Media (DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to PVDF membranes using the iBLOT2 transfer system (Thermo Scientific, USA) with iBLOT2 PVDF transfer stacks (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Sediments on the microscope slides were fixed in acetic acid/alcohol for 15 minutes and subjected to Epredia™ Papanicolaou EA Staining (22-050-211, Thermo Fisher Scientific, USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... with iBLOT2 PVDF transfer stacks (Thermo Scientific, USA). Membranes were blocked with 3% BSA (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... All purified protein: Fractions containing glutathione reductase were combined and desalted using 5 or 10 ml Zeba™ Spin desalting columns (Thermo Scientific™, USA) into 50 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... coli BL21(DE3)(Life Technologies, USA) and purified using affinity chromatography ...
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bioRxiv - Cell Biology 2024Quote: ... His-tagged: The lysate was mixed head-over-head with Ni-NTA agarose resin (Thermo Scientific™, USA) for 60 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... gels using MOPS SDS running buffer (Thermo Scientific, USA). Proteins were transferred to PVDF membranes using the iBLOT2 transfer system (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were selected by addition of 2 µg/ml Puromycin (Gibco) for 10 days ...
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bioRxiv - Cell Biology 2024Quote: ... Lysate was adjusted to equal protein concentration across samples and diluted with 4x NuPAGE LDS sample buffer (Thermo Scientific, USA) containing 50 mM DTT (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... DMEM supplemented with 2% Horse serum (Gibco, 16050-122) and 1% Penicillin-Streptomycin was given to C2C12 myoblasts to induce differentiation ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were separated on 4-12 % NuPAGE BisTris (Thermo Scientific, USA) gels using MOPS SDS running buffer (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... bFGF (5 ng/ml) (Gibco, PHG0021), 10 µM ROCK inhibitor (MedChemExpress ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration was determined using a Pierce™ BCA assay kit (Thermo Scientific™, USA). Total GSH levels ...
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bioRxiv - Cell Biology 2024Quote: ... For analysis a Q-Exactive HF-X mass spectrometer (Thermo Fisher Scientific) in positive ion mode was used with full scan analysis over a range of m/z[400– 1600[m/z at 60,000 resolution ...
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bioRxiv - Cell Biology 2024Quote: ... 1X Penicillin/Streptamycin (Gibco, 15140122). Monocytes were allowed to adhere to substrate for 5 days at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... in Dulbecco’s Modified Eagle Media (DMEM; Gibco, 31-053-028).
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bioRxiv - Cell Biology 2024Quote: ... or glass-bottom culture dishes (Nunc, 150680) in complete DMEM consisting of 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged proteins were concentrated using 30K MWCO concentrators to ∼ 5 mg/ml (Thermo Scientific™, USA). Protein was stored at −80°C.
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration was determined using a Pierce™ BCA assay kit (Thermo Scientific, USA). Lysate was adjusted to equal protein concentration across samples and diluted with 4x NuPAGE LDS sample buffer (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 30 µL of Protein A/G magnetic agarose (Thermo Scientific, 78609) was incubated with 15 µg of TDP-43 antibody (Proteintech ...
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bioRxiv - Cell Biology 2024Quote: ... a Thermo Orbitrap QExactive (Thermo Fisher Scientific) was used.
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bioRxiv - Cell Biology 2024Quote: ... the signal was detected using enhanced chemiluminescence (ECL, Thermofisher) and images were acquired with Intas Chemiluminescence imager (Science imaging ...
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bioRxiv - Cell Biology 2024Quote: Hydrophilic interaction liquid chromatography (HILIC) was carried out on a Dionex UltiMate 3000 RSLC system (Thermo Fisher Scientific, Hemel Hempstead, UK) using a ZIC-pHILIC column (150 mm × 4.6 mm ...
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bioRxiv - Cell Biology 2024Quote: ... Plsamodium falciparum 3D7 strain was cultured under standard culture conditions in RPMI media supplemented with 0.5% (W/V) Albumax (Invitrogen). To generate the HA-glmS transgenic parasite lines ...
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bioRxiv - Cell Biology 2024Quote: ... protocol and these dry peptides residues were solubilized in 20 µL 5 % acetonitrile with 0.5 % formic acid using the auto-sampler of a nanoflow uHPLC system (Thermo Scientific RSLCnano). Online detection of peptide ions was by electrospray ionisation (ESI ...
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bioRxiv - Cell Biology 2024Quote: ... Peptide separation was performed on a Pepmap C18 reversed phase column (50 cm × 75 µm, particle size 3 µm, pore size 100 Å, Thermo Scientific) using a solvent gradient at a fixed solvent flow rate of 0.3 μl / min for the analytical column ...
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bioRxiv - Cell Biology 2024Quote: ... The absorbance of wells was subsequently measured at 450 nm using a Multiskan FC microplate reader (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA). Following 24-or 48-h incubations ...
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bioRxiv - Cell Biology 2024Quote: ... Rabbit anti-IP3R1 at 1:200 (Thermo Fisher, Cat# PA1-901, RRID:AB_2129984), and Mouse anti-KDEL at 1:500 (Enzo Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... 1% GlutaMAX (Thermo Fisher 35050061), 0.1% BME (Invitrogen 21985-023) ...
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bioRxiv - Cell Biology 2024Quote: ... Stock solutions of calcein acetomethyl ester (CAM; 5 mg/ml) (Thermo Fisher Scientific, C3100MP), propidium iodide (PI ...