Labshake search
Citations for Thermo Fisher :
1 - 50 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used for staining were donkey anti-mouse or anti-rabbit Alexa 488 (Invitrogen), donkey anti-rabbit or anti-mouse Cy3 (Jackson Immunoresearch) ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were mounted in Prolong Diamond with DAPI (Invitrogen) and imaged using a Leica SP8 confocal microscope.
-
bioRxiv - Cell Biology 2024Quote: ... 30pmol/ml CAPS siRNA hs.Ri.CAPS.13.3 (IDT) was transfected into cells using either RNAiMax (Invitrogen) or Lipofectamine 3000 (Invtrogen ...
-
bioRxiv - Cell Biology 2024Quote: ... 12 ng/mL bFGF (Gibco, 13256-029), and 1% knockout serum replacement (KSR ...
-
bioRxiv - Cell Biology 2024Quote: ... according to the manufacturer’s instructions or extracted with TRIzolβ reagent (Thermo Fisher Scientific, 15596018). Reverse transcription was performed using a qScriptTM cDNA SuperMix kit (Quantabio ...
-
bioRxiv - Cell Biology 2024Quote: ... 1×Insulin-Transferrin-Selenium (ThermoFisher Scientific, 41400045) and 15% KSR for additional 2 weeks ...
-
bioRxiv - Pathology 2024Quote: ... and goat anti-mouse Alexa Fluor 546 (Invitrogen: A-11030) were used ...
-
bioRxiv - Pathology 2024Quote: ... TMT-labeled samples were combined and desalted using 100 µl Pierce® C18 Tips (Thermo Fisher Scientific, Cat#87784) and dried in an Vacufuge concentrator (Eppendorf 5301) ...
-
bioRxiv - Pathology 2024Quote: ... the peptide was reconstituted in 2% acetonitrile/0.1% formic acid (FA) and analyzed by an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific) coupled online to an UltiMate 3000 RSLCnano System (Thermo Fisher Scientific) ...
-
bioRxiv - Pathology 2024Quote: ... TRIzol Reagent (Invitrogen, 15596026) was added to flasks and frozen at −80°C ...
-
bioRxiv - Pathology 2024Quote: ... The concentration of the peptides was determined using Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Cat#23275). Tandem mass tag (TMT)-based labeling of peptides was performed using the TMT10plex™ Isobaric Label Reagent Set (Thermo Fisher Scientific ...
-
bioRxiv - Pathology 2024Quote: ... coupled online to an UltiMate 3000 RSLCnano System (Thermo Fisher Scientific). Briefly ...
-
bioRxiv - Pathology 2024Quote: Samples were lysed in RIPA buffer (Thermo Scientific) supplemented with 1% Halt Protease and Phosphatase Inhibitor Cocktail ...
-
bioRxiv - Pathology 2024Quote: ... 1:100 CD36 Super Bright 600 (Invitrogen: 63-0362-82), 1:200 CD45-Alexafluor700 (Biolegend ...
-
bioRxiv - Pathology 2024Quote: ... and incubated with 1 µM LysoTracker (Excitation at 405 nm) Dye (Life Technologies) for 30 minutes at 37°C ...
-
bioRxiv - Pathology 2024Quote: ... TRIzol Reagent (Invitrogen, 15596026) was added to flasks and frozen at −80°C ...
-
bioRxiv - Pathology 2024Quote: ... Protein lysates (500 μg) from each biological sample were incubated with 100 μL of NeutrAvidin Agarose Resins (Thermo Fisher, Cat# 29200) with rotation for 1 hour at room temperature ...
-
bioRxiv - Pathology 2024Quote: ... cells were treated with Dil conjugated oxLDL (Invitrogen: L34358) at a concentration of 10ug/mL for 24 hours ...
-
bioRxiv - Pathology 2024Quote: ... Staining was performed in FACS buffer at the following dilutions: 1:1000 LipidTox Red (Thermo Scientific: H34476), 1:100 CD36 Super Bright 600 (Invitrogen ...
-
bioRxiv - Cell Biology 2024Quote: Total RNA was isolated using TRIzol (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... qPCR was performed using Platinum SYBR Green (Invitrogen) and an AriaMX Real-Time PCR machine (Agilent) ...
-
bioRxiv - Cell Biology 2024Quote: ... and a mixture of 80 pmol random primers (Invitrogen) and 20 pmol oligo d(T)20VN (Merck) ...
-
bioRxiv - Cell Biology 2024Quote: ... RNA extracts were treated with TURBO DNase (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by cDNA synthesis from 2 µg total RNA using SuperScript III reverse transcriptase (Invitrogen) and a mixture of 80 pmol random primers (Invitrogen ...
-
bioRxiv - Cancer Biology 2024Quote: ... placed in gradual freezing aid (Thermo Fisher, #5100-0001), and moved to -80°C before transferal to liquid nitrogen for long-term storage ...
-
bioRxiv - Cancer Biology 2024Quote: ... for 2D cultures and TrypLE (Invitrogen, 12604-013) for 3D cultures ...
-
bioRxiv - Cancer Biology 2024Quote: ... KRAS expression was validated in HEK293T cells via Western Blot after transfection with PEI (Thermo Fisher, BMS1003-A). The ASPS model was generated in a collaborative project with Priya Chudasama on “Immunogenomics characterization of alveolar soft part sarcoma”.
-
bioRxiv - Cancer Biology 2024Quote: ... were suspended in Synth-a-Freeze Cryopreservation Medium (Gibco, A1254201), placed in gradual freezing aid (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2024Quote: ... Five guides per target gene were tested in vitro for their editing efficiency in N2A cells after transfection using Lipofectamine 3000 (Thermo Fisher, L3000001). Where available previously published guides were included ...
-
bioRxiv - Cancer Biology 2024Quote: ... or a spheroid TSM-complete for 3D culture (Peterziel et al., 2022) into 6 well plates (Greiner, 657160 for adherent culture and Thermo Fisher, 174932 for suspension culture). Collagen coating was employed for 2D culture to facilitate attachment for the first passage ...
-
bioRxiv - Cancer Biology 2024Quote: ... all cell lines were maintained in DMEM (Gibco) supplemented with 10% fetal calf serum (FCS ...
-
bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, 10270-106) and 1% Zell Shield (Minerva Biolabs GmbH ...
-
bioRxiv - Cancer Biology 2024Quote: ... mouse EGF (Gibco, Jenks, OK) was added 10 minutes prior to harvesting protein lysates from treated tumor cell lines to assess the impact of upstream receptor tyrosine kinase activation of RAS-MAPK/AKT signaling.
-
bioRxiv - Cancer Biology 2024Quote: ... pH 7.2) with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific), and clarified at 16,000 RCF 15 minutes at 4°C ...
-
bioRxiv - Plant Biology 2024Quote: ... SimplyBlue SafeStain (465034, Invitrogen) was used to visualize the amount of rubisco on the PVDF membrane.
-
bioRxiv - Plant Biology 2024Quote: ... 2.5 uL 10X sybr green 1 (Life Technologies), 2.5 uL ImmoBuffer™ (Bioline ...
-
bioRxiv - Plant Biology 2024Quote: ... Total RNA was treated with TURBO DNA-free kit (Invitrogen). The integrity of the RNA was checked on 1% (w/v ...
-
bioRxiv - Plant Biology 2024Quote: ... 500 ng of total RNA was spiked with 10 pg of Luciferase RNA and reverse-transcribed using SuperScript IV kit using random primers (Thermo Scientific). cDNAs were then diluted 50-fold prior to ddPCR analysis ...
-
Diurnal regulation of SOS Pathway and Sodium Excretion Underlying Salinity Tolerance of Vigna marinabioRxiv - Plant Biology 2024Quote: ... From the extracted RNA we prepared libraries for 3’mRNA-seq with Collibri 3’mRNA Library Preparation Kit for Illumina Systems (Thermo Fisher Scientific K.K., Tokyo, Japan). The prepared libraries were sequenced with Hiseq4000 as a customer service of GeneBay Inc ...
-
bioRxiv - Cancer Biology 2024Quote: ... OVCAR8 (RRID:CVCL_1629) were cultured in RPMI-1640 medium (Gibco, Thermo Fisher Scientific, Cat. #11875) plus 10% heat-inactivated fetal bovine serum (Phoenix Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... OVCAR8 (RRID:CVCL_1629) were cultured in RPMI-1640 medium (Gibco, Thermo Fisher Scientific, Cat. #11875) plus 10% heat-inactivated fetal bovine serum (Phoenix Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID:CVCL_6911) were culture similarly but in DMEM medium (Gibco, Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID:CVCL_6911) were culture similarly but in DMEM medium (Gibco, Thermo Fisher Scientific, Cat. #11995040). pCDH-EF1a-mCherry-EGFP-LC3B (RRID ...
-
bioRxiv - Cancer Biology 2024Quote: ... Mouse anti-human claudin-4 (Thermo Fisher Scientific Cat# 32-9400 ...
-
bioRxiv - Plant Biology 2024Quote: ... Target sites were amplified using Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific, F537S) from wt and transgenic lines ...
-
bioRxiv - Plant Biology 2024Quote: ... The ZEP3-NosT PCR product was sub-cloned into the pJET vector using the Blunt-End Cloning Protocol (ThermoFisher K1232) and the PCR product of primers 13 and 14 (SI Table 1 ...
-
bioRxiv - Plant Biology 2024Quote: ... Selection was performed by plating transformants on f/2 + 10 μg/mL blasticidin S HCl (ThermoFisher Scientific) 1% agar plates under 24 h 80 μmol photons m−2 s−1 light conditions.
-
bioRxiv - Plant Biology 2024Quote: ... Total RNA was then extracted from the tissue powder using TRIzol Reagent (Invitrogen, Cat# 15596-018) following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2024Quote: ... then redissolved in 100 μL hexane for GC-MS (ISQ-7000, Thermo Scientific, USA). The GC run sequence starts with an initial hold at 110 °C for 2 minutes ...
-
bioRxiv - Plant Biology 2024Quote: ... After digesting with the NcoI (Thermo Scientific) restriction enzyme ...