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Citations for Thermo Fisher :
1 - 50 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... with RPMI medium 1640 medium (Gibco-Thermo Fisher Scientific) supplemented with fetal bovine serum (10%) ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with correspondent secondary antibodies (Invitrogen) and nuclear counterstaining for 1h at RT ...
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bioRxiv - Microbiology 2024Quote: ... after which incubated with a secondary antibody goat anti-rabbit Alexa Fluor 488 (1:800, Thermo Scientific) for 1h ...
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bioRxiv - Microbiology 2024Quote: ... phalloidin (Invitrogen, A12380) or wheat germ agglutinin (WGA ...
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bioRxiv - Microbiology 2024Quote: ... that was coupled to Easy nLC (Proxeon Biosystems, now Thermo Fisher Scientific). The raw data for each sample were searched using the MASCOT engine (Matrix Science ...
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bioRxiv - Microbiology 2024Quote: ... The extracts obtained were prepared at a concentration of 1 mg/mL and subjected to analysis via liquid chromatography-high resolution mass spectrometry/mass spectrometry (LC-HRMS/MS) using a (Thermo Scientific QExactive® Quadrupole-Orbitrap Hybrid instrument) ...
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bioRxiv - Microbiology 2024Quote: ... 4°C (Thermo Fisher Sorvall Legend RT Centrifuge). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5M EDTA pH 8.0 (Invitrogen, Thermo Fisher Scientific). Cells were plated in 24 or 48-well culture plates ...
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bioRxiv - Microbiology 2024Quote: ... and fluorescence images of each well were acquired using a high-content imager (Arrayscan XTI, Thermo Fisher Scientific). Viral titers were determined by counting the number of focus-forming units (FFU ...
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bioRxiv - Microbiology 2024Quote: ... Proteomics analysis was then carried out by LC-MS/MS system with on a Q Exactive mass spectrometer (Thermo Scientific) that was coupled to Easy nLC (Proxeon Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... We used RNaseZap RNase Decontamination Solution (Invitrogen AM9780) on all surfaces to prevent RNA degradation ...
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bioRxiv - Microbiology 2024Quote: ... by measuring absorbance at 254 nm and 400 μl fractions were collected in 96 wells plates (Thermo Scientific)
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bioRxiv - Microbiology 2024Quote: ... After pre-incubation for 1 hour at 42 °C in the hybridization buffer (ULTRAhyb, Invitrogen Thermo Fisher Scientific), membranes were incubated with the probe ...
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bioRxiv - Microbiology 2024Quote: ... subtilis cells were fixed with 4% Paraformaldehyde (PFA; Thermo Scientific), 0.02% Glutaraldehyde directly in the resuspension buffer for 15 min at room temperature ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... (86)]was maintained in Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 (DMEM/F-12, high glucose; Gibco) supplemented with 5% Serum Plus (Sigma Aldrich) ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... After an additional two days of incubation the plates were supplemented with fresh complete media and 3ul of Hoechst dye (ThermoFisher Scientific, Madison, WI, USA) was added to sensitize the cells ...
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bioRxiv - Microbiology 2024Quote: Both PCR product sets were purified with 0.8× HighPrepTM PCR magnetic beads (MagBio, USA) and quantified using a NanoDropTM Lite Spectrophotometer (Thermo Fisher Scientific Inc., USA). Samples (200 ηg each ...
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bioRxiv - Microbiology 2024Quote: ... RNA quality was assessed through agarose gel electrophoresis and quantified using a NanoDropTM Lite Spectrophotometer (Thermo Fisher Scientific Inc., USA).
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bioRxiv - Microbiology 2024Quote: ... External calibration was performed with pepmix XT kit (Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... following the instructions available in the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems®). Real-time Polymerase Chain Reaction (qPCR ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... Oligos were pooled and labelled with 250 μM Amino-11-dUTP (Thermo Scientific) and TdT enzyme (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... and blocked at room temperature with StartingBlock PBS Blocking Buffer (Thermo Scientific). Membrane was blotted with anti-ChmA (dilution = 1:500 ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... 50 mg/ml gentamicin (Gibco) and 1 mg/ml doxycycline for the purposes of repression ...
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bioRxiv - Microbiology 2024Quote: ... Real-time Polymerase Chain Reaction (qPCR) was conducted in a StepOnePlus™ Real-time PCR system (Applied Biosystems®) to analyze the relative expression of the genes of inducible nitric oxide synthase (iNOS ...
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bioRxiv - Microbiology 2024Quote: ... The coverslips were then incubated with FluoSpheres beads (0.1 µm, 1:106 dilution; Life Technologies) for 10 min as fiducial markers ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... (85)] were maintained in Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 (DMEM/F-12, high glucose; Gibco) supplemented with 5% Serum Plus (Sigma Aldrich ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... Samples were washed with 0.1% Tween 20 in PBS 3 times and cover slips were affixed to slides using ProLong Gold Antifade Mountant (Thermo Scientific). Fibers were then imaged with a Leica Stellaris confocal microscope using a 63X oil immersion objective lens ...
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bioRxiv - Microbiology 2024Quote: ... 30 μL of each concentrated sample was run on a Bolt 4–12% Bis-Tris Plus Gel (Thermo Scientific, catalog number ...
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bioRxiv - Microbiology 2024Quote: ... or α-Mouse (Invitrogen, cat# 62-6520) secondary antibody at a titer of 1:5000 for 45 minutes at room temperature ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... and 50 ug/ml penicillin/streptomycin (Gibco). HepG2-NTCP cells were maintained on collagen coated dishes for optimal growth and morphological characteristics ...
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bioRxiv - Microbiology 2024Quote: ... then chemiluminescence was detected using a Chemiluminescent Nucleic Acid Detection Module kit (Thermo Scientific) by exposure on a Tanon-5200 Multi instrument (Tanon Science & Technology Co ...
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bioRxiv - Microbiology 2024Quote: ... The extension products were then analysed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific, MA, USA), and the results were viewed using Peak Scanner Software v1.0.
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bioRxiv - Microbiology 2024Quote: ... anti-caveolin 1 mouse mAb (7C8) (Thermo Fisher #MA3-600), anti-GFP tag rabbit polyclonal antibody (Proteintech #50430-2-AP) ...
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bioRxiv - Microbiology 2024Quote: ... 150ng of the donor vector containing the EBV cDNA and the destination vector were co-incubated with 1X LR Clonase Enzyme Mix (Invitrogen #11789-020) overnight at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... incubated with ECL chemiluminescence solution (Thermo Fisher #34578) and images were captured by Licor Fc platform ...
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bioRxiv - Microbiology 2024Quote: ... glass (2 gm, 3 mm bead diameter; Fisher Scientific) and polystyrene (24-well plates ...
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bioRxiv - Microbiology 2024Quote: ... and the protein concentration was determined using Pierce™ BCA Protein Assay Kits (Thermo Fisher Scientific, cat# 23225). The lysates were subsequently reduced with 1 × Protein Loading buffer (TransGen ...
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bioRxiv - Microbiology 2024Quote: ... the samples were transferred onto a membrane using an iBlot 2 NC Mini Stack device (Invitrogen) and the following protocol ...
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bioRxiv - Microbiology 2024Quote: ... and added to magnetic Protein G beads (Invitrogen, Cat# 10003D) coated with mouse monoclonal anti-Pys25 antibody (96 ...
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bioRxiv - Microbiology 2024Quote: ... or streptavidin-HRP (1:1000 dilution, Fisher Scientific, Novex Cat# 43-423-3) to ensure that biotinylated and bait proteins were present ...
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bioRxiv - Microbiology 2024Quote: ... was purchased from American Type Cell Collection (ATCC) and cultured in eagle’s minimal essential medium (MEM) supplemented with 10% fetal bovine serum (Invitrogen) and 1% penicillin/streptomycin and L-glutamine ...
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bioRxiv - Microbiology 2024Quote: ... followed by a peptide clean-up on SPE spin columns before TMT labeling with the TMTpro™ 16plex Label Reagent Set (Thermo Fisher, Cat# A44520). Peptides from each sample were labeled with a distinct TMTpro™ isobaric tag before mixing ...
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bioRxiv - Microbiology 2024Quote: ... probed with anti-GFPmut2 primary antibody (1:1000 dilution, rabbit pAb, custom Pocono RF&L) with anti-rabbit conjugated to HRP (1:1000 dilution, Invitrogen, Cat #A16104) or streptavidin-HRP (1:1000 dilution ...
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bioRxiv - Microbiology 2024Quote: ... Affinity blots were imaged with Pierce™ ECL Western Blotting Substrate solution (Thermo Scientific, Cat# 32106) and imaged with BioRad Gel Dox XR Imaging System.
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bioRxiv - Microbiology 2024Quote: ... Coverslips were mounted with ProLong Gold Antifade mountant (Invitrogen catalog # P36934). For each condition ...
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bioRxiv - Microbiology 2024Quote: ... or rabbit (Donkey anti-rabbit AF594, Invitrogen, Cat# A11012). Cells were washed in 1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... Vero cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco catalog number 11965118) containing 5% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2024Quote: ... All bacterial stocks were stored as aliquots at -80°C in brain heart infusion media (BHI; Fisher Scientific, DF0037-17-8) to minimize freeze-thaw cycles ...
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bioRxiv - Microbiology 2024Quote: Plasmids containing Icm/Dot translocated substrate genes fused to gfp were constructed by inserting fragments into pDONR221 (Invitrogen) and transferring the inserts into pDEST53 (pCMV-GFP ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were grown to around 75% confluency before trypsinization with 0.05% Trypsin-EDTA (25300-054, Thermo Scientific, USA), followed by washing 3x with phosphate-buffered-saline (PBS) ...