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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from 2×106 cells (3 biological replicates for wild-type and CNOT3-DM cells) using Trizol reagent (Thermo Fisher Scientific, USA) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Total RNA was prepared from tissue samples (3-5 mg) or cell pellets (2.5×105 cells) using the Ambion PureLink RNA minikit (Life Technologies, Carlsbad, CA, USA) as directed by the supplier ...
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bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDs was harvested 96 h after infection ...
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bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDwt or RBDmut was harvested 96 h after infection ...
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bioRxiv - Molecular Biology 2024Quote: C2C12 cells (Shanghai Cell Bank, Chinese Academy of Sciences, passages 3-8) were cultured in Dulbecco modified Eagle medium (DMEM) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2024Quote: Mid-log LdAQP-GFP cells at a density of approximately 3×106 cells per mL were incubated with Mitotracker (Invitrogen/Molecular Probes, #M7512) at a final concentration of 40 nM during 15 min at 27°C in culture medium without FCS ...
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bioRxiv - Cancer Biology 2024Quote: ... The number of viable cells was determined using the Invitrogen™ Countess™ 3 FL Automated Cell Counter (Thermo Fisher Scientific, cat# AMQAX2000). Cells were seeded into a 6-well plate at a seeding density of 2e5 viable cells per mL of OncoPro medium with a final volume of 2 mL per well ...
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bioRxiv - Neuroscience 2022Quote: ... 10% 3 kD or 10 kD biotinylated dextran amines (3 kD BDA, Invitrogen D7135 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... #2: 5’-gatcccGATTACTCGGCCATGATCAAAgcttcctgtcacTTTGATCATGGCCGAGTAATCttt ttta-3’ and 5’-agcttaaaaaaGATTACTCGGCCATGATCAAAgtgacaggaagcTTTGATCATGGCCGAGTAATgg-3’ were purchased from Invitrogen. The DNA oligo pairs were annealed and inserted into pEntryCla12-chickU6 shuttle vector using BamHI/HindIII site.
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bioRxiv - Cell Biology 2020Quote: ... Lipin-1 siRNA 5’-GAAUGGAAUGCCAGCUGAA-3’ and 3’-UUCAGCUGGCAUUCCAUUC-5’ (Invitrogen; HSS118307 (Sigma); optineurin siRNA (Invitrogen 4392420) ...
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bioRxiv - Bioengineering 2023Quote: ... 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (EDC; 22980) was purchased from Thermofisher Scientific (MA) ...
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bioRxiv - Bioengineering 2021Quote: All experiments used a previously described research cell bank of T-REx-293 human embryonic kidney (HEK) cells stably expressing the tetracycline repressor protein (ThermoFisher) (4) ...
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bioRxiv - Cancer Biology 2020Quote: Cell fractionation experiments were performed using the Subcellular Protein Fractionation Kit for Cultured Cells according to manufacturer’s instructions (Thermo Fisher). Membrane fraction’s protein content was normalized by using anti-transferrin receptor antibody (Invitrogen).
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bioRxiv - Cell Biology 2019Quote: ... The cell pellet was then either processed with the Subcellular Protein Fractionation Kit for Cultured Cells kit (Thermo Scientific, 78840), or resuspended in 1 x RIPA buffer with protease inhibitors to extract whole cell lysate ...
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bioRxiv - Immunology 2022Quote: ... The sgRNA was ordered as modified synthetic sgRNA from Synthego and delivered to HeLa cells by electroporating 200,000 cells with 4 ug TrueCut Cas9 protein (ThermoFisher Scientific) complexed with 180 pmol GBP1 sgRNA using a Neon system (ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Cells were maintained on 6-well plates or cell culture flasks coated with Attachment Factor Protein (Gibco cat. No. S006100) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... Expi293F™ cells (Homo sapiens, embryonic kidney cells in suspension) used for transient protein expression were purchased (ThermoFisher Scientific, A14527) and cultured in Expi293™ Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: A549 and Calu-3 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with Glutamax (Gibco),10% fetal bovine serum ...
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bioRxiv - Cell Biology 2020Quote: ... 3×104 hTERT-RPE cells expressing mKO2-PACT were seeded into 8 well glass bottom dishes (Nunc™ Lab-Tek™ II Chambered Coverglass ...
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bioRxiv - Cell Biology 2020Quote: Cells were washed 3 times with PBS and lysed in 2x LDS sample buffer (Thermo Fisher, #B0008) supplemented with reducing reagent and 1x phosphatase and protease inhibitor ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were passaged 1:10 every 2–3 days with 1× trypsin-EDTA 0.25% (Gibco 25200-056).
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bioRxiv - Systems Biology 2020Quote: ... cells were stained with a modified Wright-Giemsa stain (HEMA 3 STAT PACK, Fisher Scientific; Waltham, MA). Cell distribution was analyzed by manually identifying and counting eosinophils ...
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bioRxiv - Molecular Biology 2022Quote: ... HCT116 cells complemented with chromosomes 3 and 5 were cultured with 400 μg/mL geneticin (G418, Gibco) and 6 ug/mL blasticidine (Invivogen) ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... The suspension of pellet 2+3 was filtered through 40-μm cell strainer (Fisher scientific, Cat # 22363547), followed by final wash in complete Ham’s F10 medium ...
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bioRxiv - Immunology 2021Quote: ... Cells were then resuspended in 10 µM CellEvent Caspase 3/7 activity reporter in PBS (Invitrogen, #C10723) and incubated for 30 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Staining of cells was performed using 5 μl of DiBAC4(3) (Invitrogen, 0.025 mg/ml in DMSO) followed by incubation for 15 minutes at room temperature in dark ...
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bioRxiv - Cell Biology 2019Quote: ... For differentiation cells were switched to M254 medium for 3-4 population doublings (ThermoFisher Scientific, Life Technologies).
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bioRxiv - Cell Biology 2019Quote: ... For differentiation cells were switched to M254 medium for 3-4 population doublings (ThermoFisher Scientific, Life Technologies).
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bioRxiv - Cell Biology 2019Quote: ... 3 different siRNAs Silencer per gene were transfected in cells using Lipofectamine 2000 (ThermoFisher Scientifics, 11668-019) at the final concentration of 10 nM ...
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bioRxiv - Immunology 2019Quote: ... purified cells were washed once with PBS and stained for 3 min with 2.5 μM CFSE (ThermoFisher). After quenching the excess CFSE by addition of 1 mL FBS for 30 seconds ...
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bioRxiv - Microbiology 2020Quote: ... the growth medium was removed by aspiration and the cells were washed with 3 mL PBS (Gibco), before adding 3 mL of Cell Stripper (Corning) ...
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bioRxiv - Bioengineering 2020Quote: ... HUVECs were used between passage 1-3 Cells were enzymatically dissociated using TrypLE (ThermoFisher Scientific, MA, USA) for 5-7 minutes and centrifuged at 250G for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: The viability of the cells was examined by staining with 1 µM TOTO-3 iodide (Molecular Probes), which only stains dead cells (Zuliani et al. ...
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bioRxiv - Bioengineering 2021Quote: ... Human lung (Calu-3) (ATCC, HTB-55) cells were cultured in Minimum Essential Media (MEM) (Gibco; 11095080) supplemented with 10% FBS with 1mM sodium pyruvate (Gibco ...
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bioRxiv - Bioengineering 2021Quote: T cells from 3 donors were thawed and activated using Dynabeads Human T-Activator CD3/CD28 (ThermoFisher) for 24 hours ...
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bioRxiv - Microbiology 2020Quote: Calu-3 cells (kind gift from Dr. Manfred Frey, Mannheim, Germany) were maintained in DMEM (Gibco, 41965039) with 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged after reaching 70-85% confluency (every 3-4 days) using Accutase (Gibco #A11105-01) to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632 ...
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bioRxiv - Cell Biology 2021Quote: ... as previously described.13 Cells were passaged every 3-4 days using Collagenase Type IV (Life Technologies). Expression of pluripotency markers was analyzed by flow cytometry using SOX2 (Cell Signaling ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were washed 3 times with PBS and visualized with EVOS FL imaging system (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... All cells were passaged 2-3 times per week with 0.25% Trypsin/0.02% EDTA (Gibco Cat#25200056). Cells used in all experimental set-ups were between passage 5 and 30.
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bioRxiv - Microbiology 2022Quote: ... Cells were washed 3 times in PBST then placed in secondary antibody rabbit IgG (A48254; Thermo Fisher) diluted in the blocking buffer for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... the infected cells were fixed and stained using a Hema 3™ Fixative and Solutions (Fisher Scientific). Syncytia were recorded using a light microscope ...
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bioRxiv - Developmental Biology 2022Quote: ... On day 3-9 cells were fed daily with Essential 6 medium (E6, #A1516401, Thermo Fisher Scientific) in the presence of 100 nM LDN193189 (#72142 ...
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bioRxiv - Neuroscience 2021Quote: Cell viability was assessed using a Human caspase-3 (active) ELISA kit (#KHO1091; Thermo Fisher Scientific, MA) following manufacture’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... media collected from infected cells and were mixed 1:3 (v/v) with TRIzol Reagent (ThermoFisher scientific) followed by chloroform extraction ...
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bioRxiv - Immunology 2020Quote: ... 105 hCD3+ T cells were mixed with human T-Activator CD3/CD28 Dynabeads (Thermofisher, ratio 3:1) or PHA (1μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... A549 or Vero cells were transfected with 3 μg of plasmids with transfection reagent LipofectamineR 2000 (Invitrogen) according to manufacturers’ instructions 24 h post seeding ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then washed 3 times in 1X PBS and incubated in Alexa 488 anti-rabbit (Invitrogen) diluted 1:1000 in 5% bovine serum albumin in 0.2% Triton X-100 in PBS for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Fixed cells were then washed with PBS and stained with 3 µM DAPI (Thermo Scientific, Waltham, MA). Cells were then analyzed with a FACSAria flow cytometer (BD Bioscience ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were washed with TBS (3×10min) and mounted with ProLong Gold antifade mountant (P10144, Thermo Scientific). Primary antibodies used in this study were ...