Labshake search
Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
-
bioRxiv - Developmental Biology 2021Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449 ...
-
bioRxiv - Plant Biology 2023Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours with rotation ...
-
bioRxiv - Immunology 2022Quote: ... Protein A or protein G Dynabeads (Invitrogen) were prepared according to manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... Protein A and Protein G Dynabeads (Invitrogen) premixed at 1:1 ratio were used for pre-clearing for 1 hour at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
-
bioRxiv - Immunology 2020Quote: ... Samples were run on NativePAGE 3-12% BisTris Protein Gels using the dark blue cathode protocol for the NativePAGE™ Novex® Bis-Tris Gel system (Thermo Fisher Scientific). Gels were fixed ...
-
bioRxiv - Bioengineering 2020Quote: Purified S-ECD protein was biotinylated at a theoretical 1:3 molar ratio with EZ-Link NHS-PEG12-Biotin (Thermo Fisher Scientific, CAT#: 21313) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
-
bioRxiv - Immunology 2023Quote: ... The filtered supernatants were concentrated 8-fold using a pre-rinsed 3K MWCO Pierce Protein Concentrator PES (molecular-weight cutoff: 3 kDa) (Thermo Fisher Scientific, 88525, USA) by centrifuging at 4000 rcf in 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... mixed with forward and reverse detection primers (T3DCD S1 forward [5’- TACGCGTTGATCACGACAAT-3’] and T3DCD S1 reverse [5’- TGGCGAGATTATTCCCTGAC-3’] or GAPDH forward [5’- ACCCAGAAGACTGTGGATGG-3’] and GAPDH reverse [5’- GGATGCAGGGATGATGTTCT-3’]) and SYBR Select Master Mix (Applied Biosystems), and then subjected to PCR using the StepOnePlus Real-Time PCR system (Applied Biosystems) ...
-
bioRxiv - Microbiology 2021Quote: ... and customized si-KIF4 3′ UTR targeting endogenous KIF4 mRNA 3’-UTR region (5′-GGAAUGAGGUUGUGAUCUUTT-3′) were purchased from Thermo Fisher Scientific.
-
bioRxiv - Cell Biology 2020Quote: ... reverse: 5’-GGGGTCGGGAGGAACGG-3’ (Macrogen, South Korea) and beta-actin forward: 5’-CCTGGTCGGTTTGATGTT-3’ and reverse: 5’-GTGCGACGAAGACGA-3’ (Invitrogen, USA). Data analysis was made in CFX ManagerTM Software (BioRad ...
-
The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... FW 5’-CTCTCTGGGCGAAATCTGCT-3’ and REV 5’-GAGTGCTTTCGCTATGTTGTTCA-3’ for Clmn and FW 5’-TGATGGGTGTGAACCACGAG-3’ and REV 5’-GCCGTATTCATTGTCATACCAGG-3’ for Gapdh using a QuantStudio 7 (Applied Biosystems). Transcript levels are expressed as the ratio of 2−ΔCT (Transcript of interest)/2−ΔCT (Gapdh).
-
bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted from freshly-dissected n=3 young (3-month-old) or n=3 aged (18-month-old) zebrafish brain in TRIzol (Invitrogen, 15596). Three independent experimental replicates were used for bulk RNA sequencing ...
-
bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...
-
bioRxiv - Cell Biology 2020Quote: ... total protein was determined by homogenising the cells in RIPA buffer (Life Technologies 89900) containing protease inhibitor cocktail (Sigma 11836153001) ...
-
bioRxiv - Cell Biology 2020Quote: Whole-cell lysates were prepared in M-PER mammalian protein extraction reagent (ThermoFisher Scientific) containing a Halt™ protease inhibitor cocktail (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... and RBD proteins were purified from the supernatants of transiently-transfected Expi293F cells (Gibco) by nickel affinity and size-exclusion chromatography26 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Subcellular protein fractionation was performed using a kit for cultured cells from Thermo Fisher Scientific (78840) ...
-
bioRxiv - Molecular Biology 2019Quote: 6xHis 12-heptad SRS fusion proteins were expressed in SoluBL21 Escherichia coli cells (Invitrogen) from a pet42a vector ...
-
bioRxiv - Cancer Biology 2019Quote: ... Biotinylated proteins were isolated by incubating the cell lysates with Avidin-coated beads (ThermoFisher) for 1h at 4°C ...
-
bioRxiv - Bioengineering 2021Quote: ... N-protein specific mAb CR3009 was produced by co-transfecting Expi293F cells (Life Technologies) in suspension growing at 37oC in 8% CO2 atmosphere in FreeStyle 293T medium (Life Technologies ...
-
bioRxiv - Biochemistry 2021Quote: ... protein was extracted from cells or retinas with Pierce RIPA buffer (89900; Life Technologies) supplied with protease inhibitor cocktail (11697498001 ...
-
bioRxiv - Microbiology 2020Quote: THP-1 cells were lysed in M-PER mammalian protein extraction reagent (Thermo Scientific) supplemented with Pierce protease inhibitor (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... or by tryptic shaving of cell-surface proteins by using recombinant enzymes (TrypLE, Gibco). Three biological replicates of cells cultured in the presence or absence of serum ...
-
bioRxiv - Bioengineering 2022Quote: ... total proteins from hCMEC/D3 cells were extracted using RIPA buffer (89900, Fisher Scientific) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
-
bioRxiv - Bioengineering 2022Quote: ... the cells were transfected with Spike protein expressing pLP plasmids using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol and stained for their spike protein expression 72 hours after the transfection as described in Staining and Flow cytometry Analysis ...
-
bioRxiv - Immunology 2022Quote: ... The proteins were captured from the clarified cell culture using TALON resin (ThermoFisher Scientific) and eluted with imidazole ...
-
bioRxiv - Immunology 2022Quote: ... The protein was expressed in mammalian Expi293F cells (Thermo Fisher Scientific, Cat no. A14527) and purified using Ni-NTA (GE Healthcare ...
-
bioRxiv - Molecular Biology 2020Quote: ... flow-aligned cells were lysed with M-PER mammalian protein extraction reagent (Thermofisher, MA) supplemented with 1% protease and phosphatase inhibitor cocktail (Thermofisher ...
-
bioRxiv - Cell Biology 2021Quote: ... The antibodies-cell lysates were then incubated with Protein G Dynabeads (10003D, ThermoFisher Scientific) for 2 hours ...
-
bioRxiv - Cell Biology 2021Quote: Cells were lysed in M-PER™ Mammalian Protein Extraction Reagent (Thermo Scientific, Waltham) containing a protease inhibitor mixture (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2020Quote: Cells were lysed using M-PER™ Mammalian Protein Extraction Reagent (78503, Thermo Scientific) supplemented with Halt™ Protease Inhibitor Cocktail (87785 ...
-
bioRxiv - Biochemistry 2021Quote: ... N-protein specific mAb CR3009 was produced by co-transfecting Expi293F cells (Life Technologies) in suspension growing at 37oC in 8% CO2 atmosphere in FreeStyle 293T medium (Life Technologies ...
-
bioRxiv - Microbiology 2021Quote: 500.000 cells were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific 78501) containing 1x protease inhibitor (cOmplete Tablets ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were extracted by cell lysis buffer (NP40 or RIPA Buffer, respectively, Thermo Scientific) and completed with Proteases Inhibitors (Complete PIC ...
-
bioRxiv - Immunology 2022Quote: ... The protein was expressed in mammalian Expi293F cells (Thermo Fisher Scientific, Cat no. A14527) and purified using Ni-NTA (GE Healthcare ...
-
bioRxiv - Evolutionary Biology 2022Quote: The PierceTM Cell Surface Protein Biotinylation and Isolation Kit (Thermo Fisher Scientific, Massachusetts USA) was used to quantify total and cell surface expression levels of N-terminal GFP- tagged TadNaC2 protein expressed in CHO-K1 cells from the pEGFP-TadNaC2 vector ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... 30µg cell protein was separated on a 10% Bis-Tris gel (NP0301PK2, ThermoFisher, Inc) and blotted onto nitrocellulose membranes using the standard protocol provided by the manufacturer ...
-
bioRxiv - Developmental Biology 2019Quote: ... Cells were cultured on 6-well plates coated with Vitronectin Recombinant Human Protein (Invitrogen) at a concentration of 0.5 µg/cm2 ...
-
bioRxiv - Cell Biology 2019Quote: ... cell lysates (∼12 mg total protein) were incubated with anti-TAP antibody (ThermoFisher #CAB1001) for 4 h at 4°C ...
-
bioRxiv - Cancer Biology 2019Quote: Cells were lysed on-plate using Mammalian Protein Extraction Reagent (M-PER; Thermo Scientific), with Halt Protease Inhibitor Cocktail (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2019Quote: ... The Cas12a fusion protein was expressed in BL21 star (DE3) cells (Thermo Fisher Scientific) using a previously established protocol with minor modifications (Strohkendl et al. ...
-
bioRxiv - Microbiology 2021Quote: Extractions were performed with the Subcellular Protein Fractionation Kit for Cultured Cells (Thermo scientific) following the manufacturer’s recommendations ...
-
bioRxiv - Cancer Biology 2020Quote: Cells were biochemically fractionated using a subcellular protein fractionation kit (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: Cells were washed with DPBS and lysed with Mammalian Protein Extraction Reagent (Thermo Fisher) with 1X Halt’s Protease and Phosphatase (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2020Quote: ... The protein quantities in cell extracts were measured with a BCA kit (ThermoFisher Scientific). The proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...