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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... The proteins were extracted in NP-40 cell lysis buffer (Thermo Fisher Scientific, #FNN0021) with HaltTM phosphatase inhibitor Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... cell surface proteins were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant RBD proteins were produced in transfected FreeStyle 293F cells (Invitrogen, Carlsbad, CA, USA) and purified by nickel affinity chromatography ...
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bioRxiv - Microbiology 2021Quote: ... mSpyCatcher protein was purified from transiently transfected Expi293F cells following manufacturer’s guidelines (Gibco, Thermofisher) as described previously37 ...
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bioRxiv - Microbiology 2021Quote: ... mSpyCatcher protein was purified from transiently transfected Expi293F cells following manufacturer’s guidelines (Gibco, Thermofisher) as described previously37 ...
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bioRxiv - Cell Biology 2021Quote: ... Cytosolic protein lysates from MIN6 cells were precleared with 20 µg yeast tRNA (Ambion) and 50 µl Dynabeads by rotating at 4°C for 1 h ...
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bioRxiv - Biophysics 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were stained by incubating cells with Alexa Fluor 647-conjugated streptavidin (ThermoFisher). Coverslips were prepared for imaging by mounting in Vectashield liquid mounting media (Vector Labs).
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bioRxiv - Immunology 2022Quote: ... the transfected cells were supplemented with Protein-Free Hybridoma Medium Supernatant (PFHM-II, Gibco). The supernatant containing secreted mAb was harvested at day 4 and purified using protein A-agarose beads (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... cells were fractionated using a subcellular protein fractionation kit (Thermo Scientific cat. no. 78840). Eluted proteins from subcellular fractions were quantified by a Pierce ™ BCA protein assay kit (Cat.no ...
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bioRxiv - Neuroscience 2023Quote: ... cells were harvested and lysed in M-per mammalian protein extraction reagent (Thermo scientific) with protease inhibitor (EDTA-free ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell lysate was pre-cleared with Protein G Sepharose (115544935, Thermo Fisher Scientific) for 2 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... For 100,000 cells 6.25 pmol TrueCut TM Cas9 Protein v2 (Thermo Fisher Cat: A36496) and 12.5 pmol of sgRNA (Synthego ...
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bioRxiv - Immunology 2022Quote: ... Proteins were then produced in human embryonic kidney (HEK)293F cells (Thermo Fisher Scientific) maintained in Freestyle medium (Life Technologies) ...
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bioRxiv - Cell Biology 2024Quote: Protein was isolated from cells using the RIPA Lysis and Extraction Buffer (Thermo Scientific) with the following specifications ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed using the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) following manufacturer’s recommendations ...
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bioRxiv - Biophysics 2024Quote: Protein was expressed using Expi293F cells grown in Expi293 Expression medium (Thermo Fisher Scientific). 2 L Cells were grown in 5 L Optimum growth flasks (Thompson ...
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bioRxiv - Pathology 2024Quote: Total protein was extracted from cultured cells using Pierce RIPA Buffer (Thermo Fisher Scientific) containing 1% Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Cell protein extracts were resolved by Bis-Tris polyacrylamide gel electrophoresis gels (Life Technologies) and run in 1 X MOPS buffer (Life Technologies) ...
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bioRxiv - Immunology 2024Quote: ... Proteins were expressed transiently under chemically defined conditions in Freestyle 293F-cells (Thermo Scientific). His-tagged proteins were purified from purified supernatants using immobilized metal affinity chromatography followed by size-exclusion chromatography (SEC) ...
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bioRxiv - Immunology 2023Quote: ... Both FL S and RBD protein expressions were conducted in Expi293F cells (ThermoFisher Scientific) grown in Expi293 medium ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were lysed and biotinylated proteins immunoprecipitated with streptavidin-conjugated agarose beads (ThermoFisher Scientific) overnight at 4°C with agitation ...
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bioRxiv - Bioengineering 2023Quote: ... Then PBMCs were cultured with 1x Cell Stimulation Cocktail plus protein transport inhibitors (Invitrogen) for 4 hrs ...
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bioRxiv - Microbiology 2023Quote: ... Specific epithelial cell proteins were detected by immunoblotting with anti-c-Met (#370100, Invitrogen), anti-EGFR (#2234 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant BA.5 and BQ.1.1 RBD proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations of cell lysates were determined with the bicinchoninic acid (Thermo Fisher, 23225) assay and normalized to 2 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: Cells were lysed by M-PER mammalian protein extraction reagent (Thermo Scientific, Cat# 78501), protein quantified by BCA protein assay ( Thermo scientific #23250) ...
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bioRxiv - Neuroscience 2023Quote: ... slices underwent protein synthesis labeling using the Click-iT Cell Reaction Buffer Kit (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... SOSIP proteins were expressed in transiently transfected HEK293F suspension cells (Invitrogen, cat no. R79009), maintained in FreeStyle Expression Medium (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: ... antibody was purified on Protein A resin from clarified cell supernatant (Thermo Fisher Scientific). Large-scale production of 10-1074 was conducted at Scripps Research Center for Antibody Development & Production (La Jolla ...
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bioRxiv - Genomics 2022Quote: ... 40ug of whole cell protein lysate was incubated with LDS loading buffer (Thermo Fisher, CAT#NP0007 ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were produced in ExpiCHO cells transfected with Expifectamine™ reagent (Thermo Fisher Scientific) as per manufacturer’s protocol41 ...
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bioRxiv - Physiology 2023Quote: Total protein was isolated from cells or mouse tissue using RIPA buffer (Thermo Fisher), or lysates were enriched for histone proteins using Histone Extraction Kit (Abcam) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed in mammalian protein extraction reagent (MPER, Thermo Fisher Scientific, Waltham, MA) containing a protease and phosphatase inhibitor cocktail (Sigma-Aldrich Inc. ...
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bioRxiv - Biochemistry 2023Quote: Protein extracts from cells and tissues were obtained with RIPA Buffer (Thermo Scientific, # 89900). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were pre-cleared using PierceTM Protein A Magnetic beads (#88846) from ThermoFisher for 1 hour at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... The cells were transfected with the Cas9 enzyme (TrueCut Cas9 Protein v2, Thermo Fisher) and the sgRNA using Lipofectamine™ CRISPRMAX™ Transfection Reagent (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: Tissues or cells were lysed using N-PER Neuronal Protein Extraction Reagent (Thermo Fisher), supplemented with both a protease and phosphatase inhibitor cocktail (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... cell surface proteins were biotinylated using EZ-Link Sulfo-NHS-Biotin (Thermo Scientific, 21217). Media was aspirated from wells and cells rinsed with PBS ...
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bioRxiv - Microbiology 2024Quote: ... Whole cell extracts were resolved on 4-12% gradient Bis-Tris Protein Gels (ThermoFisher) and transferred to Polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysates were combined with 50 µl of Protein G Dynabeads (ThermoFisher, 10004D), equilibrated in TNN ...
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bioRxiv - Microbiology 2019Quote: ... Cells were lysed by boiling in 1M NaOH and protein quantified using the Micro BCA™ Protein Assay (Thermo-Fisher Scientific Inc., Waltham, U.S.A.) and a VersaMax™ microplate reader (Molecular Devices LLC ...
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bioRxiv - Genomics 2021Quote: ... Cell pellets corresponding to 10 million cells were washed twice with ice-cold PBS and subjected to subcellular protein extractions using a Subcellular Protein Fractionation for Cultured Cells kit (Thermo Fisher Scientific, TFS #78840) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates containing approximately 20 µg of protein were resolved on Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen, Waltham, MA). The separated proteins were transferred to nitrocellulose membranes that were subse-quently blocked using 5% non-fat dry milk diluted in TBST (10 mM Tris-HCL ...
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bioRxiv - Cell Biology 2024Quote: ... cells were collected by centrifugation and total protein extracts were obtained using M-PER™ Mammalian Protein Extraction Reagent (Thermo-Fisher Scientific, CA, USA) supplemented with pierce protease kit and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... cells scraped off and transferred to a 1.5 ml tube for cell lysis before clearance by centrifugation and protein concentration measurement using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) following manufactureŕs instructions and the FLUOstar Omega reader ...
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bioRxiv - Systems Biology 2024Quote: ... and the concentration of total protein in cell extracts was determined using the Qubit® protein assay kit and the Qubit® 3.0 fluorometer (Thermo Fisher Scientific™). Cell culture supernatants were used purely ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total protein content in BAL fluid after cell removal was assessed using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... The peptide amount of tumor cell samples was estimated using a fluorescence based protein assay (Qubit Protein assay, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...