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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cell surface proteins were labeled by incubating cells with 1.6 mM Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific, Waltham, MA USA) or 1.6 mM Biotin-dPEG3-Maleimide (MilliporeSigma ...
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bioRxiv - Immunology 2022Quote: ... cells were re-suspended in a 1X dilution of Cell Stimulation Cocktail Plus Protein Transport Inhibitors (Invitrogen, #00-4975-93, CA) in cRPMI for 2 hours at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: MC4R enhancer knockout cells were generated by transfecting WTC11-NGN2 iPSC cells in 6-well plates with 6.25ug Cas9 protein (Fisher Scientific, A36498) and 800ng sgRNAs (IDT) ...
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bioRxiv - Immunology 2023Quote: ... MC38-Cas9 cells were generated by infecting MC38 cells with lentivirus encoding Cas9 protein and selected by blasticidin S (Gibco A1113903). MC38-EGFP and MC38-Gal-1-EGFP cell lines were generated by infecting MC38-Cas9 cells with EGFP-expressing and Gal-1-EGFP-expressing lentivirus ...
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bioRxiv - Cell Biology 2019Quote: ... The pelleted cells were lysed using B-PER II bacterial protein extraction reagent (Thermo Fisher) for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... Biotinylated proteins were isolated by incubating the cell lysates with Avidin-coated beads (ThermoFisher Scientifc) for 1h at 4°C ...
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bioRxiv - Immunology 2020Quote: ... An additional 50 µL of 5× eBioscience Cell Stimulation Cocktail with protein transporter inhibitor (ThermoFisher) was added and cells were treated for an additional 4 hr at 37 °C.
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bioRxiv - Immunology 2019Quote: Cell lysates of sorted HSPCs were separated by 4-12% Bis-Tris Protein Gels (Invitrogen) and transferred to PVDF transfer membranes (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates (20 μg protein) were separated on NuPAGE 4–12% Bis-Tris Gels (Invitrogen) and transferred to polyvinyl difluoride membranes ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cell extracts were prepared with B-PER Complete Bacterial Protein Extraction Reagent (Thermo Fisher Scientific) and the amount of protein was measured with the Bradford Protein Assay (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lysates were pre-cleared with 20 μL protein A Dynabeads (Life Technologies, Carlsbad, CA) for 30 minutes at 4°C prior to incubation with the indicated antibody overnight at 4°C in total volume of 1mL MCLB supplemented with 1 μL RNAseOUT (Invitrogen ...
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bioRxiv - Immunology 2021Quote: Whole cell lysate extracts were prepared M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78503) supplemented with 1X Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Sub-cellular fractionation of cells was performed using the subcellular protein fractionation kit (Thermo Scientific) and each extract was subjected to immunoblotting with indicated antibodies.
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bioRxiv - Cell Biology 2021Quote: ... the cells were placed in PFHMII protein free medium (GIBCO, ThermoFisher Scientific, Waltham, MA, USA). After 3 days (D-3) ...
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bioRxiv - Physiology 2020Quote: ... Biotinylated cell surface proteins were affinity-purified using NeutrAvidin-conjugated agarose beads (Thermo Fisher Scientific), and purified cell surface proteins were analyzed by western blot using the mαNaVPAN antibody (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates (20 µg) were run on NuPAGE 4-12% Bis-Tris protein gels (Invitrogen) using standard procedures ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration of the whole cell extract was determined using the NanoDrop (Thermo Scientific). Equal amounts of whole cell extract were added to SDS PAGE gel wells ...
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bioRxiv - Molecular Biology 2020Quote: Cytosolic RNAs were isolated from cell lysates using subcellular protein fractionation kit (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Cells harvested for protein extraction were lysed in Radioimmunoprecipitation Assay buffer (RIPA buffer; Thermo Fisher) supplemented with 1X protease inhibitors (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell protein extracts were resolved by Bis-Tris polyacrylamide gel electrophoresis gels (Thermo Fisher Scientific) and run in either MOPS or MES buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were placed in PFHMII protein free medium (GIBCO, ThermoFisher Scientific, Waltham, MA, USA). After 3 days (D-3) ...
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bioRxiv - Molecular Biology 2022Quote: Cells used for protein blot were lysed using RIPA buffer (Life Technologies, Cat no. 89901) and sonicated for 5 seconds three times at power set of 50% (VirTis VIRSONIC 100) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were expressed by transfection of plasmids into Expi293 cells using Expifectamine transfection reagent (ThermoFisher) in suspension at 37°C for 4-5 days ...
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bioRxiv - Pathology 2021Quote: ... Cell lysates were incubated with protein A/G magnetic beads (88802, Thermo Fisher Scientific, USA) and either mouse IgG (5415S ...
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bioRxiv - Molecular Biology 2020Quote: ... total proteins were extracted from HEK293T cells using RIPA Lysis and Extraction Buffer (Thermo Scientific) supplemented with Protease Inhibitor Cocktail for Use with Mammalian Cell and Tissue Extracts (Nakalai Tesque ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were placed in PFHMII protein free medium (GIBCO, ThermoFisher Scientific, Waltham, MA, USA). After 3 days (D-3) ...
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bioRxiv - Microbiology 2021Quote: ... Pre-assembled Cas9-gRNA protein-RNA complexes were electroporated into cells using Neon equipment (ThermoFisher). A CXCR4 gRNA was always included as an additional control ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed in RIPA buffer and total protein determined using BCA (Thermo Life Technologies).
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bioRxiv - Immunology 2022Quote: ... BMDMs and THP-1 cells were lysed in mammalian protein extraction reagent (MPER, Thermo Scientific) along with cOmplete Mini (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were produced intracellularly in Sf9 cells following the Bac-to-Bac protocol (ThermoFisher Scientific). Cells were lysed by sonication or using a ball bearing bore homogenizer ...
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bioRxiv - Molecular Biology 2019Quote: ... and CRISPR/Cas9 protein were delivered to cells by electroporation with Neon transfection system (ThermoFisher). Clones were screened by PCR and Miseq sequencing (Illumina).
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentration in the whole cell extracts was determined with the BCA colorimetric assay (Thermofisher) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Total cell lysate protein concentrations were determined with the Better Bradford Kit (Thermo-Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Protein concentration in cell lysates was quantified using Bradford assay (Thermo Fisher Scientific, Waltham, MA). Equal amounts of protein were then separated by SDS-PAGE under reducing conditions ...
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bioRxiv - Microbiology 2020Quote: Cell surface proteins were biotinylated using an EZ-Link Sulfo-NHS-Biotin reagent (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Harvested cell pellets were resuspended in mammalian protein extraction reagent (Thermo Fisher Scientific, NY, USA) containing protease inhibitor mixture (Roche ...
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bioRxiv - Immunology 2021Quote: ... Proteins in the cell lysates were separated on 4-12% Bis-Tris precast gels (Invitrogen) in MES buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Protein contents of total cell lysates were determined by Pierce BCA assay (Thermo Fisher Scientific) using a Synergy 4 fluorescence microplate reader (Biotek ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested and lysed with B-PER® bacterial protein extraction reagent (Thermo Scientific). The total protein level was checked by the Bradford assay kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Assay was performed according to protocol utilizing Pierce Cell Surface Protein Isolation Kit (Thermo Fisher) with the following modifications ...
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bioRxiv - Immunology 2021Quote: ... Proteins were extracted from 50 million cells using the MEM-PER extraction kit (Thermo Fisher). Soluble proteins (1.5 ml ...
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bioRxiv - Cell Biology 2022Quote: ... the concentrations of cell lysate were measured using Micro BCA Protein Assay Kit (Thermo Scientific). About 30-40 μg total proteins were analyzed by SDS-PAGE and quantitative Western blotting ...
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bioRxiv - Cancer Biology 2022Quote: ... Fractionation assays were conducted with the “Subcellular Protein Fractionation Kit for Cultured Cells” (Thermo Fisher) and performed as advised by the manufacturer ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentration in total cell lysate was calculated using the BCA Gold Assay (ThermoFisher, A53226). After protein concentration was determined ...
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bioRxiv - Neuroscience 2022Quote: Cell lysates were homogenized in a cocktail of Mammalian Protein Extraction Reagent (MPER, ThermoFisher Scientific) and 1:10 HALT Protease and Phosphatase Inhibitor (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... The cell lysate-Ab was then added to 50 μl of protein G Dynabeads (Invitrogen) for 6 hrs on a rotator at 40C ...
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bioRxiv - Molecular Biology 2024Quote: ... and whole cell extract was quantified using the BCA Protein Assay Kit (Life Technologies, 23250) following manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellet was discarded and the Pierce BCA Protein Assay kit (Thermo Scientific, #23225) was used to quantify protein lysate concentration.
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bioRxiv - Neuroscience 2023Quote: Total proteins were extracted by cell lysis in RIPA lysis buffer (Thermo Scientific, Cat. # 89901) supplemented with protease (Roche ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific™, Cat# 78501) supplemented with Protease Inhibitor Cocktail and Halt Phosphatase Cocktail (Thermo Scientific™ ...