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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... antibody was purified on Protein A resin from clarified cell supernatant (Thermo Fisher Scientific). Large-scale production of 10-1074 was conducted at Scripps Research Center for Antibody Development & Production (La Jolla ...
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bioRxiv - Genomics 2022Quote: ... 40ug of whole cell protein lysate was incubated with LDS loading buffer (Thermo Fisher, CAT#NP0007 ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were produced in ExpiCHO cells transfected with Expifectamine™ reagent (Thermo Fisher Scientific) as per manufacturer’s protocol41 ...
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bioRxiv - Physiology 2023Quote: Total protein was isolated from cells or mouse tissue using RIPA buffer (Thermo Fisher), or lysates were enriched for histone proteins using Histone Extraction Kit (Abcam) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed in mammalian protein extraction reagent (MPER, Thermo Fisher Scientific, Waltham, MA) containing a protease and phosphatase inhibitor cocktail (Sigma-Aldrich Inc. ...
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bioRxiv - Biochemistry 2023Quote: Protein extracts from cells and tissues were obtained with RIPA Buffer (Thermo Scientific, # 89900). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Both FL S and RBD protein expressions were conducted in Expi293F cells (ThermoFisher Scientific) grown in Expi293 medium ...
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bioRxiv - Pathology 2024Quote: Total protein was extracted from cultured cells using Pierce RIPA Buffer (Thermo Fisher Scientific) containing 1% Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: Protein was isolated from cells using the RIPA Lysis and Extraction Buffer (Thermo Scientific) with the following specifications ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed using the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) following manufacturer’s recommendations ...
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bioRxiv - Biophysics 2024Quote: Protein was expressed using Expi293F cells grown in Expi293 Expression medium (Thermo Fisher Scientific). 2 L Cells were grown in 5 L Optimum growth flasks (Thompson ...
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bioRxiv - Cell Biology 2024Quote: ... Cell protein extracts were resolved by Bis-Tris polyacrylamide gel electrophoresis gels (Life Technologies) and run in 1 X MOPS buffer (Life Technologies) ...
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bioRxiv - Immunology 2024Quote: ... Proteins were expressed transiently under chemically defined conditions in Freestyle 293F-cells (Thermo Scientific). His-tagged proteins were purified from purified supernatants using immobilized metal affinity chromatography followed by size-exclusion chromatography (SEC) ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell lysate was pre-cleared with Protein G Sepharose (115544935, Thermo Fisher Scientific) for 2 h at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were lysed and biotinylated proteins immunoprecipitated with streptavidin-conjugated agarose beads (ThermoFisher Scientific) overnight at 4°C with agitation ...
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bioRxiv - Microbiology 2019Quote: ... Cells were lysed by boiling in 1M NaOH and protein quantified using the Micro BCA™ Protein Assay (Thermo-Fisher Scientific Inc., Waltham, U.S.A.) and a VersaMax™ microplate reader (Molecular Devices LLC ...
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bioRxiv - Genomics 2021Quote: ... Cell pellets corresponding to 10 million cells were washed twice with ice-cold PBS and subjected to subcellular protein extractions using a Subcellular Protein Fractionation for Cultured Cells kit (Thermo Fisher Scientific, TFS #78840) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The cell lysates containing approximately 20 µg of protein were resolved on Novex™ NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen, Waltham, MA). The separated proteins were transferred to nitrocellulose membranes that were subse-quently blocked using 5% non-fat dry milk diluted in TBST (10 mM Tris-HCL ...
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bioRxiv - Neuroscience 2023Quote: ... The peptide amount of tumor cell samples was estimated using a fluorescence based protein assay (Qubit Protein assay, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...
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bioRxiv - Systems Biology 2024Quote: ... and the concentration of total protein in cell extracts was determined using the Qubit® protein assay kit and the Qubit® 3.0 fluorometer (Thermo Fisher Scientific™). Cell culture supernatants were used purely ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total protein content in BAL fluid after cell removal was assessed using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, USA) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... cells were collected by centrifugation and total protein extracts were obtained using M-PER™ Mammalian Protein Extraction Reagent (Thermo-Fisher Scientific, CA, USA) supplemented with pierce protease kit and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... cells scraped off and transferred to a 1.5 ml tube for cell lysis before clearance by centrifugation and protein concentration measurement using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) following manufactureŕs instructions and the FLUOstar Omega reader ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from 2×106 cells (3 biological replicates for wild-type and CNOT3-DM cells) using Trizol reagent (Thermo Fisher Scientific, USA) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Total RNA was prepared from tissue samples (3-5 mg) or cell pellets (2.5×105 cells) using the Ambion PureLink RNA minikit (Life Technologies, Carlsbad, CA, USA) as directed by the supplier ...
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bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDs was harvested 96 h after infection ...
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bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDwt or RBDmut was harvested 96 h after infection ...
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bioRxiv - Molecular Biology 2024Quote: C2C12 cells (Shanghai Cell Bank, Chinese Academy of Sciences, passages 3-8) were cultured in Dulbecco modified Eagle medium (DMEM) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2024Quote: Mid-log LdAQP-GFP cells at a density of approximately 3×106 cells per mL were incubated with Mitotracker (Invitrogen/Molecular Probes, #M7512) at a final concentration of 40 nM during 15 min at 27°C in culture medium without FCS ...
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bioRxiv - Neuroscience 2022Quote: ... 10% 3 kD or 10 kD biotinylated dextran amines (3 kD BDA, Invitrogen D7135 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... #2: 5’-gatcccGATTACTCGGCCATGATCAAAgcttcctgtcacTTTGATCATGGCCGAGTAATCttt ttta-3’ and 5’-agcttaaaaaaGATTACTCGGCCATGATCAAAgtgacaggaagcTTTGATCATGGCCGAGTAATgg-3’ were purchased from Invitrogen. The DNA oligo pairs were annealed and inserted into pEntryCla12-chickU6 shuttle vector using BamHI/HindIII site.
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bioRxiv - Cell Biology 2020Quote: ... Lipin-1 siRNA 5’-GAAUGGAAUGCCAGCUGAA-3’ and 3’-UUCAGCUGGCAUUCCAUUC-5’ (Invitrogen; HSS118307 (Sigma); optineurin siRNA (Invitrogen 4392420) ...
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bioRxiv - Bioengineering 2023Quote: ... 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (EDC; 22980) was purchased from Thermofisher Scientific (MA) ...
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bioRxiv - Bioengineering 2021Quote: All experiments used a previously described research cell bank of T-REx-293 human embryonic kidney (HEK) cells stably expressing the tetracycline repressor protein (ThermoFisher) (4) ...
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bioRxiv - Cancer Biology 2020Quote: Cell fractionation experiments were performed using the Subcellular Protein Fractionation Kit for Cultured Cells according to manufacturer’s instructions (Thermo Fisher). Membrane fraction’s protein content was normalized by using anti-transferrin receptor antibody (Invitrogen).
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bioRxiv - Cell Biology 2019Quote: ... The cell pellet was then either processed with the Subcellular Protein Fractionation Kit for Cultured Cells kit (Thermo Scientific, 78840), or resuspended in 1 x RIPA buffer with protease inhibitors to extract whole cell lysate ...
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bioRxiv - Immunology 2022Quote: ... The sgRNA was ordered as modified synthetic sgRNA from Synthego and delivered to HeLa cells by electroporating 200,000 cells with 4 ug TrueCut Cas9 protein (ThermoFisher Scientific) complexed with 180 pmol GBP1 sgRNA using a Neon system (ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Cells were maintained on 6-well plates or cell culture flasks coated with Attachment Factor Protein (Gibco cat. No. S006100) at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: A549 and Calu-3 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with Glutamax (Gibco),10% fetal bovine serum ...
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bioRxiv - Cell Biology 2020Quote: ... 3×104 hTERT-RPE cells expressing mKO2-PACT were seeded into 8 well glass bottom dishes (Nunc™ Lab-Tek™ II Chambered Coverglass ...
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bioRxiv - Cell Biology 2020Quote: Cells were washed 3 times with PBS and lysed in 2x LDS sample buffer (Thermo Fisher, #B0008) supplemented with reducing reagent and 1x phosphatase and protease inhibitor ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were passaged 1:10 every 2–3 days with 1× trypsin-EDTA 0.25% (Gibco 25200-056).
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bioRxiv - Systems Biology 2020Quote: ... cells were stained with a modified Wright-Giemsa stain (HEMA 3 STAT PACK, Fisher Scientific; Waltham, MA). Cell distribution was analyzed by manually identifying and counting eosinophils ...
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bioRxiv - Molecular Biology 2022Quote: ... HCT116 cells complemented with chromosomes 3 and 5 were cultured with 400 μg/mL geneticin (G418, Gibco) and 6 ug/mL blasticidine (Invivogen) ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... The suspension of pellet 2+3 was filtered through 40-μm cell strainer (Fisher scientific, Cat # 22363547), followed by final wash in complete Ham’s F10 medium ...
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bioRxiv - Immunology 2021Quote: ... Cells were then resuspended in 10 µM CellEvent Caspase 3/7 activity reporter in PBS (Invitrogen, #C10723) and incubated for 30 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Staining of cells was performed using 5 μl of DiBAC4(3) (Invitrogen, 0.025 mg/ml in DMSO) followed by incubation for 15 minutes at room temperature in dark ...
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bioRxiv - Cell Biology 2019Quote: ... For differentiation cells were switched to M254 medium for 3-4 population doublings (ThermoFisher Scientific, Life Technologies).
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bioRxiv - Cell Biology 2019Quote: ... For differentiation cells were switched to M254 medium for 3-4 population doublings (ThermoFisher Scientific, Life Technologies).