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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 2×105 cells were transfected with preincubated 18pmol of Cas9 (TrueCut Cas9 Protein v2, ThermoFisher) and 45pmol of either SLC2A1 targeting sgRNA (5’-GGATGCTCTCCCCATAGCGG ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates from neuroblastoma cells were separated on 4-12% Bis-Tris gels (Life Technologies), transferred to a PVDF membrane ...
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bioRxiv - Cell Biology 2024Quote: ... Whole-cell protein lysates were prepared in M-PER buffer (Thermo Scientific, Rockford, IL, USA) with 1x Protease & Phosphatase Inhibitor Cocktail (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Subcellular fractionation was performed using a subcellular protein fractionation kit for cultured cells (Thermo scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... surface proteins on the cell were labelled using membrane impermeant Sulfo-NHS-SS-Biotin (ThermoFisher). The biotinylation process was carried out on ice ...
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bioRxiv - Microbiology 2023Quote: ... The cell lysates were then combined with 50 µl of Protein G Dynabeads (ThermoFisher, 10004D) equilibrated lysate buffer B and re-incubated at 4°C for 30 min with rotation ...
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bioRxiv - Genetics 2023Quote: HEK293 cell protein was extracted with 0.3 mL of RIPA buffer (ThermoFisher, Cat. No. 89900) containing Protease Inhibitor Cocktail (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: Fractionation assays were conducted with the “Subcellular Protein Fractionation Kit for Cultured Cells” (Thermo Fisher) and performed as advised by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleared whole cell lysates were incubated with GFP-antibody and Protein G Dynabeads (Life Technologies) as described above.
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bioRxiv - Immunology 2023Quote: ... Cell lysates were also subjected to a BCA assay for protein quantification (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... and XCell SureLock Mini-Cell vertical mini-protein gel electrophoresis system (EI0001, Thermo Fisher Scientific). Protein transfer was performed using polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2023Quote: ... The cell lysates were detected by a Dynabeads protein G immunoprecipitation kit (Invitrogen, USA, #10007D) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... The resuspended cells were lysed by bacterial protein extraction reagent B-PER (Thermo Fisher Scientific). In order to precipitate insoluble protein fractions and partially disrupted cells ...
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bioRxiv - Cell Biology 2023Quote: Recombinant ectodomain proteins were generated by transfection of HEK293T cell cultures using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed and each fraction was collected using Subcellular Protein Fractionation Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Brains were excised and stored for protein isolation in 1X Cell Lysis Buffer (ThermoFisher Scientific) with protease inhibitors cocktail (ThermoFisher Scientific) ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: Total protein concentration of cell lysates was determined using micro-BCA kit (Thermo Fisher 23235). Presenilin and APP proteins were separated with 12% v/v acrylamide ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein concentration in the cell lysate was determined using the BCA kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Large-scale protein expression was performed by infection of High-Five cells (Thermo Fisher, B85502) in Insect-XPRESS medium (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... phosphorylated-S6Ser240/244 Ribosomal Protein S6 (pS6; clone D68F8; Cell Signalling) and Ki67 (SolA15; Invitrogen) before detection with fluorochrome conjugated secondary antibodies (Invitrogen or Abcam) ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations for total or fractionated cell lysates were determined by BCA assay (Fisher Scientific). Equal quantities (20-50 μg ...
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bioRxiv - Cancer Biology 2023Quote: The protein concentrations in cell/tumor lysates were determined using the BCA assay (ThermoFisher, #23227). After being separated by Nu-PAGE SDS-PAGE (4-12% gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... Concentration of cleared cell lysate was assessed by the Pierce BCA Protein Assay Kit (ThermoFisher). Equal amounts of protein (20-40ug ...
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bioRxiv - Cancer Biology 2023Quote: Proteins were isolated from HCC cell lines using RIPA Lysis buffer (Thermo Fisher, cat.no. 89900) supplemented with Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... 500 μg precleared cell protein lysate was treated with 1 U RNase I (Ambion AM2294) for 5 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 5 × 10e4 C2C12 cells were electroporated with 7.5 pmol TrueCut Cas9 protein v2 (ThermoFisher Scientific) complexed with 22.5 pmol sgRNA using the Neon system (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates from cells were prepared with RIPA lysis buffer (ThermoFisher Scientific, Waltham, MA, USA) containing protease inhibitor cocktail (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... cells were lysed and protein lysates were incubated with streptavidin beads (Thermo Fisher, Monza, Italy) to isolate biotinylated proteins ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were grown to early logarithmic phase at 37 °C in Protein Expression Medium (Gibco) or M9 minimal medium supplemented with 15NH4Cl (for expressing uniformly 15N labeled protein) ...
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bioRxiv - Cell Biology 2024Quote: ... Protein content of the cells was measured using the BCA Assay Kit (Thermo Fisher Scientific) and the counts were normalized to the protein content of the cells.
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bioRxiv - Cell Biology 2024Quote: ... Protein content of the cells was measured using the BCA Assay Kit (Thermo Fisher Scientific) and the counts were normalized to the protein content of the cells.
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bioRxiv - Neuroscience 2024Quote: The protein concentration in cell lysates was assayed using a BCA gold kit (Life Technologies). Proteins were denatured (or not ...
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bioRxiv - Immunology 2024Quote: ... The extracted cells of lung tissue were plated into Attachment Factor Protein-(Life Technologies, USA) coated T75 tissue culture flasks for 2 days ...
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bioRxiv - Cell Biology 2024Quote: We prepared total protein extracts by boiling cell pellet in SDS PAGE loading buffer (Invitrogen) complemented with NuPage reducing agent (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... Cells were collected and lysed using the B-PER Bacterial Protein Extraction Reagent (ThermoFisher Scientific) with lysozyme and benzonase ...
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bioRxiv - Neuroscience 2020Quote: Total protein concentrations of isolated protein lysates were quantified by BCA protein assay kit (ThermoFisher) and equal protein amounts were boiled at 95°C for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Immunology 2021Quote: ... 3 μM Hoechst (Life Technologies) was added to each well to stain nuclei for cell counting ...
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bioRxiv - Immunology 2021Quote: ... 3 μM Hoechst (Life Technologies) was added to each well to stain nuclei for cell counting ...
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bioRxiv - Genomics 2021Quote: ... 3 mL (Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 3 washes 5x SSCT (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 washes 5x SSCT (ThermoFisher Scientific 15557044 ...
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bioRxiv - Biochemistry 2019Quote: ... 50 × 3 mm (Thermo Scientific). The mobile phase consisted of 100 mM ammonium carbonate (A ...
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bioRxiv - Cancer Biology 2019Quote: ... 3 mM glutamine (Life Technologies), 10% dialyzed fetal bovine serum (dFBS ...
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bioRxiv - Developmental Biology 2019Quote: ... and toto-3 (Life Technologies) were used after primary antibody incubation.
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bioRxiv - Immunology 2021Quote: ... supplemented with 3% FBS (Gibco) and 100 U/mL penicillin-streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... cleaved-caspase-3 (9H19L2-Invitrogen); TH (AB152-Merck Millipore) ...
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bioRxiv - Bioengineering 2020Quote: ... SP-DiOC18(3) (ThermoFisher Scientific) for 20 min ...
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bioRxiv - Neuroscience 2020Quote: sucrose (Fisher Scientific #S5-3), cellobiose [D-(+)-cellobiose ...
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bioRxiv - Neuroscience 2020Quote: ... 3 mM NaOH (Fisher Scientific) in neurobasal medium] and centrifugation at 800 × g for 5 min ...