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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: Twenty μg protein samples were loaded into lanes of a 3-8% Tris-acetate gel (cat. EA0378BOX, NuPAGE™, Invitrogen, Thermo Fisher Scientific, CA, USA) and subjected to electrophoresis ...
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bioRxiv - Systems Biology 2024Quote: ... Samples (3 μl corresponding to 600 ng of protein) were loaded on to the trapping column (Thermo Scientific, PepMap100, C18, 75 μm X 20 mm), using partial loop injection ...
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bioRxiv - Microbiology 2020Quote: Concentrated live or inactivated virus samples were mixed with Novex™ Tris-Glycine SDS Sample Buffer (2X) (Thermofisher Scientific) with NuPAGE™ Sample Reducing Agent (10X ...
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bioRxiv - Neuroscience 2020Quote: ... patient-derived fibroblasts were infected with Sendai virus using the CytoTune-iPS 2.0 Sendai Reprogramming Kit (Invitrogen, CA, US) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... RNA was reverse transcribed and amplified using TaqMan Fast Virus 1-Step Master Mix qRT-PCR Master Mix (Invitrogen) on the LightCycler 480 or LC96 instrument (Roche ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Virus titres were measured from a thawed aliquot by: (1) mixing 20 μl with 200 μl of TRIzol (Invitrogen); (2 ...
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bioRxiv - Neuroscience 2019Quote: ... PBMCs were transduced with 2.0 Sendai virus particles containing four Yamanaka factors using the CytoTune-iPS Sendai Reprogramming Kit (ThermoFisher). After seven days ...
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bioRxiv - Microbiology 2019Quote: ... Two microliters of extracted RNA was amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturer’s protocol and primers specific for HCoV-229E [30] or RSV-B [31] ...
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bioRxiv - Microbiology 2021Quote: ... Virus prepared for purification by ultracentrifugation was grown in serum-free media (VP-SFM; Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Virus prepared for purification by ultracentrifugation was grown in serum-free media (VP-SFM; Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... a homolog of the vaccinia virus H3L gene) [13] using the Path-ID™ qPCR Master Mix (Thermofisher Scientific). Nucleic acid from capripoxvirus positive samples was further analysed by a qPCR targeting LSDV011 designed to differentiate the three capripoxvirus species [14] and a qPCR targeting LSDV008 designed to differentiate LSDV wildtype strains from Neethling vaccine strains [15] ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions were performed with Taq-Man™ Fast virus 1-step master mix (ThermoFisher Scientific, Cat. 4444436) and primer-probe sets targeting SARS-CoV-2 nucleocapsid (N1 set ...
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bioRxiv - Microbiology 2021Quote: ... Virus was quantified using real-time PCR using SYBR Premix Ex Taq™ (Applied Biosystems, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Virus was quantified using real-time PCR using SYBR Premix Ex Taq™ (Applied Biosystems, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... virion concentration was determined indirectly through the DNA absorbance at 260/280nm of the virus stock (NanoDrop, Thermo Scientific). The MOI equivalent (MOI eq. ...
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bioRxiv - Cancer Biology 2020Quote: ... converted to cDNA by RT with random hexamers and Moloney murine leukemia virus (MuLV) reverse transcriptase (Thermo Fisher Scientific) and used in PCR with the following primers ...
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bioRxiv - Biophysics 2020Quote: ... PCoV_GX and RaTG13 pseudoviruses were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective spike protein into 293T cells (ATCC) ...
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bioRxiv - Microbiology 2020Quote: ... Two microliters of extracted RNA were amplified using TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... 4 x TaqMan® Fast Virus 1-Step Master Mix made up with molecular-grade nuclease free water (Ambion) to a final volume of 15 μl ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed as previously described using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and an OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were placed in a stereotaxic apparatus to receive bilateral infusions of the virus or 0.5% (w/v) cholera toxin subunit B (CTb) Alexa Fluor 555 Conjugate (Invitrogen) into the dorsal striatum (+0.1mm antero-posterior ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed according to the manufacturer’s instructions using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2022Quote: ... MRC-University of Glasgow Centre for Virus Research) were maintained in complete media (Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% Foetal Bovine Serum (FBS ...
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bioRxiv - Pathology 2022Quote: ... RNA was reverse transcribed and amplified using the TaqMan Fast Virus 1-Step Master Mix RT-qPCR kit (Invitrogen) on the LightCycler 480 or LC96 instrument (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was amplified by RT-qPCR using the Taqman Fast Virus 1-step master mix (Thermo Fisher Scientific). Primers and probes targeting SARS-CoV-2 RNA-dependent RNA polymerase were described in [25] ...
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bioRxiv - Genomics 2019Quote: ... we reprogrammed fibroblast samples from the 181 individuals in this study using non-integrative Cytotune Sendai virus (Life Technologies) (Ban et al. ...
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bioRxiv - Cell Biology 2019Quote: ... complementary DNA was then reverse transcribed from 1 μg total RNA with Moloney murine leukemia virus reverse transcriptase (Invitrogen), using random hexamer oligonucleotides for priming ...
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bioRxiv - Microbiology 2020Quote: ... The purified virus was dissolved with an equal volume of 2x Tris-Glycine SDS Sample Buffer (Thermo Fisher Scientific) and boiled for 5 min without a reducing agent ...
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bioRxiv - Cancer Biology 2019Quote: ... Expression levels of MYC and MEIS1 transcript were quantified using TaqMan Fast Virus 1-Step Master Mix (Life Technologies) using PrimeTime qPCR Probe Assays from Integrated DNA Technologies in a 3:1 ratio of primer to 5′ 6-FAM and 3′ TAMRA labeled probe ...
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bioRxiv - Microbiology 2020Quote: ... and infected at an MOI of 1 with the VSVΔG:mNeon/VSV-G virus diluted in 10 mL Opti-MEM (Life Technologies). The cells were incubated 1 hour at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... 6-well plate format) mock- or virus-infected (MOI of 0.01) were extracted using TRIzol reagent (Thermo Fisher Scientific). Superscript® II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... and then inoculated intranasally (i.n.) with 30 μL of virus (0.5 HAU) diluted in Dulbecco’s phosphate-buffered saline (DPBS) (Gibco, Loughborough UK). Mock-treated control mice were inoculated similarly with DPBS ...
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bioRxiv - Microbiology 2021Quote: ... was propagated on Vero E6 cells at a multiplicity of infection (MOI) of 0.001 in virus diluent (DMEM supplemented with 2% FBS, 1X Penicillin/Streptomycin (Gibco), 1mM sodium pyruvate (Gibco ...
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bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 N were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV and MERS-CoV pseudovirus were generated by co-transfection of human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the respective S proteins into 293T cells (ATCC ...
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bioRxiv - Immunology 2020Quote: ... and Covid-19 virus detection was performed using One-Step qRT-PCR SuperScript™ III Platinum™ (Life Technologies) targeting the gene E ...
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bioRxiv - Microbiology 2021Quote: ... Fibroblasts were reprogrammed into iPSCs by using the episomal Sendai virus approach (CytoTune-iPS 1.0 Sendai Kit, Life Technologies) to obtain hiPSCs ...
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bioRxiv - Immunology 2022Quote: ... according to manufacturer’s recommendation and qRT-PCR was performed using TaqMan Fast Virus 1-Step Mix (Applied Biosystems, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... RT-qPCR was performed as previously described using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, Cat#4444436) and a OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Microbiology 2022Quote: ... and then quantified by qRT-PCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with the TaqMan™ 2019nCoV Assay Kit v1 (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Batches of ten 8-10 weeks old female BALB/c mice were inoculated intraperitoneally (i.p) with 105 PFU of Addavax-adjuvant recombinant virus in 200 μL of phosphate-buffered saline (PBS; Gibco). At twenty-one days post immunization ...
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bioRxiv - Cell Biology 2024Quote: ... Virus was removed 16-20 hours following transduction with fresh neural media [Neurobasal medium (NBM; Life Technologies, #21103-049) supplemented with 1x N-2 supplement 1x B-27 supplement ...
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bioRxiv - Microbiology 2024Quote: ... A virus growth medium consisting of infection media supplemented with an additional 1 µg/mL trypsin-TPCK (ThermoFisher, USA) was added to the cells ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and virus pellet was resuspended in the same volume of RIPA buffer (Cat #89901, ThermoFisher, 20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Moloney murine leukemia virus (M-MLV) reverse transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor were purchased from Invitrogen (Carlsbad, CA). The Phospho-Akt (Ser473 ...
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bioRxiv - Microbiology 2023Quote: The RNA of H1N1pdm09 was extracted from virus samples using a PureLink™ viral RNA/DNA mini kit (Invitrogen). For qRT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... Virus stocks were prepared by three passages in Vero (ATCC CCL-81) in Dulbecco’s modified Eagle Medium (DMEM) (Gibco) with 1% Penicillin-Streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells infected with pLENTI-Luciferase-expressing virus (generous gift of Charlotte Kuperwasser, Tufts University) were selected with neomycin (G418, 500μg/mL, Gibco) for 2-3 weeks ...
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bioRxiv - Biophysics 2023Quote: ... Negatively stained virus particles were observed on a 30 kV scanning electron microscope (Thermo Fisher Scientific, Quanta FEG 650) in scanning transmission electron microscopy (STEM ...
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bioRxiv - Cell Biology 2023Quote: ... HT914 and HT915 iPSC lines) using the integration-free CytoTune 2.0 Sendai virus reprogramming kit (A16517, Thermo Fisher Scientific) as previously reported [43–45] ...