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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Total protein amounts of the OM cell lysates were quantified with BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein concentration of the cell lysates was then determined using Pierce™ BCA Protein Assay Kit from ThermoFisher (#23225). After 100 mM DTT was added ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein contents of total cell lysates were determined using a BCA protein assay kit (Thermo Scientific Pierce, Rockford, IL, USA). The equal amounts of protein were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Genomics 2023Quote: ... Eluted proteins and 50 μg of protein from whole cell lysate were resolved in 4-15% NuPAGE gradient gel (ThermoFisher, NP0335) using MOPS running buffer (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: Total proteins from intestinal epithelial cells or intestinal organoids were extracted with M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) with proteinase inhibitor (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... For normalization total protein concentration was measured on an aliquot of the cell lysate with the Pierce BCA protein kit (Thermo Fisher) following the manufacturer’s instructions ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... A small aliquot of cell lysate was later thawed and used for total protein quantification following the “BCA Protein Assay Kit” manual (Thermo Scientific). The same quantity (60 μL ...
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bioRxiv - Developmental Biology 2022Quote: ... 3% FBS (Gibco), 1% MEM non-essential amino acids (Gibco) ...
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bioRxiv - Genomics 2019Quote: ... 3 (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: ... DiOC2(3) (Invitrogen) was added to a final concentration of 30µM or DiSC3(5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% FBS (Invitrogen), 20 ng ml−1 EGF (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO2/3 (Invitrogen), β-catenin (BD Transduction Laboratories) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 (Applied Biosystems). We assembled forward and reverse reads using the Geneious (https://www.geneious.com ...
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bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Gibco), 0.1mM MEM-NEAA (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... Total protein was extracted by resuspending leaf tissue in 1X PBS supplemented with 3% β-mercaptoethanol and protease inhibitor cocktail (Thermo Scientific). Samples were mixed with 6X Laemmli SDS buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Solubilised proteins in a 25 μL volume were prepared and separated by electrophoresis on NativePAGE 3-12% Bis-Tris gels (Thermo Fisher Scientific) and NuPAGE Tris-Acetate buffer (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... Procaspase-3 (0.123 mg/mL protein extract) and caspase-3 (1.0 U) were assayed in 96-well microplates (Nunc™ MicroWell™ 96-Well, Thermo Scientific™ ...
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bioRxiv - Molecular Biology 2019Quote: ... either 50 µl (Figure 2A-2C, 7 and 8) or 25 µl (Figure 2D, 2E, 3-7 and 9) magnetic beads (Dynabeads protein G, Life Technologies #10004D) was used ...
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bioRxiv - Developmental Biology 2019Quote: ... and biotinylated at a molar ratio biotin/protein (3:1) for 30 min at room temperature (EZ-Link NHS-PEG4-Biotin (1189-1195, Fisher Scientific, Germany)) ...
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bioRxiv - Genetics 2021Quote: ... the same procedure was used except that 20 ug of α-HA antibody (MBL 180-3) was bound to 400 ul equilibrated Protein A agarose (Invitrogen, 15918014) by rotating at room temperature for 1h and washed 3x with extraction buffer and protein was eluted 3x with 300 ul of 1 mg/mL HA peptide (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations were adjusted to 1-3 μg/μl in lysis buffer and NuPAGE 4x LDS sample buffer (NP0007) (Thermo Fisher Scientific) was added to lysates ...
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bioRxiv - Neuroscience 2020Quote: ... Denatured samples from both the total lysates and surface fractions were separated on NuPAGE 3-8 % Tris-Acetate protein gels (Thermo Fisher Scientific) at 200 V for 40 min ...
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bioRxiv - Immunology 2020Quote: ... were mixed by adding 3 μL of each (150 pmol) and 6 μL (180 pmol) of TruCut Cas9 Protein v2 (Thermo Fisher Scientific) and incubated for 10 minutes at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... (67)) or end-binding protein 1 (EB1)-EGFP (EB1/GFP-3; (68)) were cultured in Life Technologies Opti-MEM (Invitrogen, Carlsbad, CA) containing 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: The IGFBP-derived NLS peptides (NLS-3 and NLS-5) were extracted from the bacteria using the B-Per 6xHis Fusion Protein Purification Kit (Thermo Scientific, USA), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... each well was washed 3 times by PBS and proteins were extracted using RIPA lysis buffer completed by protease inhibitors (Fisher Scientific, France) for 1h at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... tissues were wash 3 times with PBS and incubate with protein blocking solution that contain 0.5% Triton X-100 (Fisher Scientific, Hampton, NH). Then tissues were incubated in 1:250 diluted primary antibody (CD31 (PECAM-1 ...
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bioRxiv - Immunology 2020Quote: ... Cells from spleen and BMM were washed 3 times with ice-cold PBS and lysed by M-PER™ Mammalian Protein Extraction Reagent (Thermo Scientific) in the presence of protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: N2A cells were transfected as described above with BicD2-KIF tail fusions and MBNL-GFP proteins at a ratio of 2:3 in 4-well chamber slides (Thermo Fisher, 154526). 24 hours after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... the membranes were washed 3 × 5 min with TBS-T and proteins detected using Chemiluminescent Substrate (SuperSignal™ West Pico PLUS, Thermo Scientific) by acquiring images with Chemidoc (BioRad).
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bioRxiv - Microbiology 2022Quote: ... The supernatant was transferred into a fresh tube and protein concentration was measured using the Invitrogen Qubit 3 fluorometer (Thermo Fisher Scientific). Ten µg total protein were loaded per lane.
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bioRxiv - Cell Biology 2023Quote: ... The medium was then centrifuged at 3.9 k x g for 15 minutes and concentrated using a Pierce™ protein concentrator with a 3 kilodalton cut-off (ThermoFisher, 88526). The concentrated medium was diluted 1:5 with synthetic seawater before use ...
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bioRxiv - Cell Biology 2023Quote: ... gastrocnemius muscle from mice trained on a treadmill for 3 h was fractionated into the cytoplasm and nucleus using the Subcellular Protein Fractionation Kit for Tissues (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... The resolved samples were run on either NuPAGE 3–8% Tris-acetate or NuPAGE 12% Bis-Tris protein gels (Thermo Fisher Scientific) with the appropriate running buffer ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... cells were washed 3 times with PBS and stained with HCS CellMask Deep Red (ThermoFisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... the Calu-3 cells were cultured in MEM α media supplemented with 10% FBS (Gibco) and 1% Pen/Strep (0.025%) ...
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bioRxiv - Molecular Biology 2021Quote: ... Hek293T cells were transfected with Lipofectamine 3 according to manufacturer’s recommendation (Thermo Fisher, Carlsbad, CA) and expression of K273A CFTR was analyzed 48 h to 72 h after transfection.
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bioRxiv - Molecular Biology 2019Quote: ... cell collection proceeded by adding TRI Reagent (Life Technologies, 3 mL per 15 cm dish), scraping the cells off the plate ...
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bioRxiv - Bioengineering 2020Quote: ... Human cell lines PC-3 (ATCC® CRL-1435™) were grown in RPMI1640 (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Biophysics 2020Quote: ... Cells seeded on a 35mm plate were stained with Fluo-3 AM calcium indicator (Thermofisher) at 1 µM for 20 minutes incubation after which seeded cells were image on the microscope using 488nm excitation.
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bioRxiv - Cancer Biology 2020Quote: ... Single cell suspensions were obtained by 3 minutes incubation in Trypsin-EDTA 0.05% (Life technologies) and mechanical pressure ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 cells (Clinisciences, EP-CL-0054) were grown in minimum essential medium (Gibco, MEM) supplemented with glutamax (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... AsPC-1 and BxPC-3 cells were maintained in Roswell Park Memorial Institute (RPMI, Invitrogen) supplemented with 10% FBS and 4 mM L-glutamine ...
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bioRxiv - Cell Biology 2021Quote: HAEC cells (passage 3-4) were lysed in ice-cold RIPA buffer (ThermoFisher, cat# 89900) containing protease and phosphatase inhibitors (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... cells of the first aliquot were treated with N*-(3-maleimidylpropionyl) biocytin (MPB, Thermo Scientific) at a final concentration of 107 μM for 20 min on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 3 times with PBS and incubated with Alexa Fluor secondary antibodies (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... the live and dead cells were stained with 3 μM calcein-AM (ThermoFisher, Cat. # C3100MP) and 5 μM propidium iodide (PI ...
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bioRxiv - Biochemistry 2022Quote: Cells were either transfected with PEI (1 μg/3 μg DNA) or lipofectamine 2000 (ThermoFisher) diluted in OptiMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS 3 times and stained with Gold DAPI antifade (Life Technologies) under a coverslip sealed with nail polish ...