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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... cDNA of the virus genome was generated by reverse transcription (Verso cDNA Synthesis Kit, ThermoFisher Scientific) using MBTuni-12 (5′-ACGCGTGATCAGCAAAAGCAGG ...
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bioRxiv - Genomics 2023Quote: ... Real-time PCR was conducted using TaqMan Fast Virus 1-Step Master Mix from Applied Biosystems in 384-well plates ...
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bioRxiv - Biophysics 2021Quote: A commercial HEK cell suspension cell line optimized for protein expression Expi293-F (Cat. No. A14635, Gibco Life Technologies ...
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bioRxiv - Genetics 2019Quote: ... Cell surface proteins were labeled with cell membrane impermeable Sulfo-NHS-Biotin (#21217, Thermo Fisher, Waltham, MA), quenched with 100 mM glycine ...
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bioRxiv - Biochemistry 2020Quote: ... The SALM3 fusion protein expression in transfected cell was detected with Floid™ Cell Imaging system (ThermoFisher) using immunofluorescence.
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bioRxiv - Immunology 2020Quote: ... Lung CD4+ T cells were stimulated ex vivo with cell stimulation cocktail containing protein transport inhibitors (ThermoFisher) for 4 h at 37° C prior to staining ...
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bioRxiv - Immunology 2023Quote: ... cells received 50ul of complete media containing eBioscience™ Cell Stimulation Cocktail plus protein transport inhibitors (ThermoFisher) (2μl/ml ...
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bioRxiv - Cell Biology 2023Quote: ... a cell-surface biotinylation assay was performed using Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: Proteins from cells or tissues were prepared using RIPA buffer (ThermoFisher Scientific) containing protease and phosphatase inhibitors ...
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bioRxiv - Immunology 2021Quote: All recombinant proteins were expressed and purified using Expi293F cells (Life Technologies,) as described in detail previously [34 ...
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bioRxiv - Molecular Biology 2019Quote: ... 2×106 cells and 50 µg of Protein A Dynabeads (Life Technologies) were used per IP ...
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bioRxiv - Physiology 2021Quote: Proteins from cells and tissues were extracted using RIPA buffer (ThermoFisher Pierce) with 0.5M EGTA ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting proteins were expressed in Escherichia coli BL21(DE3) cells (Invitrogen) after induction by 0.5 mM isopropyl β-D-1-thiogalactopyranoside at 18°C overnight ...
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bioRxiv - Bioengineering 2020Quote: ... cells were collected in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific) and hydrolyzed in 6M HCl for 24 hours at 110°C ...
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bioRxiv - Biochemistry 2021Quote: ... The RRM protein was recombinantly expressed using BL21 Star (DE3) cells (Invitrogen) and protein expression induced using 0.5 mM IPTG at an OD600 = 0.6-0.7 for 2 hours at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... antibody was purified on Protein A resin from cell supernatant (Thermo Fisher). Fab was generated by overnight papain digestion at 37°C using immobilized papain agarose (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... The whole cell extracts were incubated with Protein A sepharose beads (Invitrogen) coated with anti-PTPIP51 or anti-Nup358 antibody for 2 h at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... PDX cells were lysed using mammalian protein extraction reagent (mPER; Thermo Scientific) buffer ...
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bioRxiv - Immunology 2021Quote: ... Proteins were expressed as inclusion bodies in BL21 (DE3) cells (Invitrogen, USA) and dissolved in 8M urea ...
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bioRxiv - Cancer Biology 2022Quote: The total cellular protein was extracted using cell lysis buffer (Invitrogen, USA) with 1X protease and phosphatase inhibitor cocktail (Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Pf12p proteins were expressed using Spodoptera frugiperda (Sf) 21 cells (Life Technologies) cultured in Insect-XPRESS Protein-free Insect Cell Medium supplemented with L-glutamine (Lonza) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed with the Bacterial Protein Extraction Reagent (BPER, Thermo Scientific) and the total protein content was determined photometrically with the Pierce bicinchoninic acid (BCA ...
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bioRxiv - Biophysics 2019Quote: ... Unlabeled protein was purified from cells grown in LB media (Fisher Scientific) and uniformly 15N- or 15N,13C-labeled proteins were purified from cells grown in M9 minimal media with 15N ammonium chloride and/or 13C D-glucose (Cambridge Isotope Labs ...
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bioRxiv - Immunology 2020Quote: Cell and tissue lysates were prepared in Protein Extraction Reagent (Thermo Fisher) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher) ...
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bioRxiv - Pathology 2020Quote: ... Proteins were expressed in 293F cells and purified by nickel column (Thermofisher) for S trimer and protein A column (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... Cell surface proteins were precipitated with Dynabeads M-280 Streptavidin (Thermo Scientific), separated using SDS-PAGE ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein was expressed in Escherichia coli BL21(DE3) cells (Life Technologies) using a pET-46 tobacco etch virus (TEV ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were then extracted from cells using B-PER (Thermo Fisher Scientific) and subjected to a secondary screen by measuring their fluorescence intensities in Ca2+-free (30 mM 3-(N-morpholino)propanesulfonic acid (MOPS) ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... Cell lysates were harvested using mammalian protein extraction reagent (MPER) (Thermo Scientific) and incubated with anti-HA resin overnight using spin columns (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed with YPER+dialyzable yeast protein extraction reagent (Thermo Scientific) and Halt Protease Inhibitor Cocktail (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: Cells were lysed in M-Per protein extraction reagent (Thermo Fisher Scientific) or RIPA buffer containing 1% NP40 supplemented with Halt protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: Cultured cells were lysed in Mammalian Protein Extraction Reagent (MPER, Thermo Scientific), supplemented with protease inhibitor and phosphatase inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were collected in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific) supplemented with Halt Protease Inhibitor Cocktail (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Cells were fractioned using a subcellular protein fractionation kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Avi/His-tagged proteins were purified from transiently-transfected Expi293F cells (Gibco) by nickel affinity chromatography and SEC (Barnes et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... the cell lysate was incubated with 15 μL Dynabeads Protein A (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were expressed in Expi293 or ExpiCHO cells (Thermo Fisher, Massachusetts USA) using manufacturer’s instructions and purified using Ni-NTA and size exclusion chromatography ...
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bioRxiv - Microbiology 2023Quote: Cells were lysed with M-PER (Mammalian Protein Extraction Reagent, Thermo Scientific) containing 1x complete protease inhibitor cocktail (Roche ...
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bioRxiv - Cancer Biology 2023Quote: Proteins were extracted from cells with RIPA buffer (Thermo Fisher, cat #89900) containing PierceTM protease inhibitor cocktail ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed in Syn-Per Synaptic Protein Extraction reagent (ThermoFisher Scientific) and centrifuged first at 1,200 x g for 10 minutes at 4°C to remove debris ...
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bioRxiv - Physiology 2022Quote: ... Protein content of cell pellets was measuring using the BCA kit (ThermoFisher). After drying the supernatant ...
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bioRxiv - Developmental Biology 2023Quote: ... Cell lysate was precleared with Protein A beads (Invitrogen #159 18-014) for 30 minutes 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested for protein using Pierce RIPA lysis buffer (Thermo Scientific) containing 1X Halt Protease & Phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: All proteins were produced by transient PEI transfection in Expi293F cells (ThermoFisher), purified from the cell supernatants with proper affinity columns and analyzed to ensure functionality ...
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bioRxiv - Molecular Biology 2023Quote: A subcellular protein fractionation kit for cultured cells (Thermo Fisher Scientific; 78840) was used for fractionation experiments according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Expi293F / 293F cells for protein expression were purchased from Gibco (ThermoFisher Scientific). Cf2Th-CD4/CCR5 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested for protein using Pierce RIPA lysis buffer (Thermo Scientific) containing 1X Halt Protease & Phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were obtained by lysing cells with RIPA buffer (89901, Thermofisher scientific) or by concentrating cell supernatants (secretomes ...
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bioRxiv - Biochemistry 2023Quote: ... For large-scale protein expression in Expi293F GnTI-cells (Thermo Fisher Scientific) in Freestyle 293 expression medium (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: Proteins from cells and tissues were extracted using RIPA buffer (ThermoFisher Pierce) with 0.5M EGTA ...