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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Total cell lysates were pre-cleared on protein A or G sepharose (Invitrogen) for 1h at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed directly in M-PER Mammalian Protein Extraction Reagent (ThermoFisher Scientific), separated on a NuPAGE 4-12% Tris-Glycine Gel (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2019Quote: Total RNA and protein were extracted from whole cells using TRIzolRT reagent (Ambion) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... All proteins were expressed in Escherichia coli one-shoot BL21star (DE3) cells (Invitrogen), as previously described (Selyutina et al. ...
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bioRxiv - Bioengineering 2019Quote: ... cells were washed once and stained with biotiny-lated Protein L (Thermo Fisher). After a subsequent wash ...
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bioRxiv - Biochemistry 2021Quote: ... Cell fractionation was carried out using the Subcellular Protein Fractionation Kit (ThermoFisher Scientific) and as described by the manufacturer ...
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bioRxiv - Biochemistry 2021Quote: ... cells were lysed in B-PER bacterial protein extraction reagent (Thermo Fisher Scientific), supplemented with TURBO DNase I (5 U/mL ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed with M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, 78501) supplemented with protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2020Quote: ... DROSHA knockout cells were transfected with the TrueCut™ Cas9 Protein v2 (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed in M-PER® mammalian protein extraction reagent (Thermo Scientific) containing protease and phosphatase inhibitors ...
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bioRxiv - Cell Biology 2021Quote: ... the Subcellular Protein Fractionation Kit for Cultured Cells (Cat. n°: 78840, Thermo Fisher) was used according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted from cell lysates using RIPA buffer (Thermofisher Scientific, USA, 89900) containing Sigmafast EDTA free protease inhibitor cocktail (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Total cell lysates were resolved in 4-12% Bis-Tris Protein Gels (Invitrogen) and transferred in for 1.5 h (80 V ...
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bioRxiv - Cell Biology 2022Quote: ... The protein in whole-cell lysates were separated by using BlotTM Gel (Invitrogen) and transferred to nitrocellulose membranes.
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bioRxiv - Molecular Biology 2022Quote: The proteins were expressed in Sf9 cells (Bac-To-Bac expression system; Invitrogen) and purified using Ni-affinity followed by Strep-Tactin affinity purification ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids expressing fusion protein were transiently transfected into Expi293F cells (Thermo Fisher Scientific) using polyethyleneimine (Polysciences) ...
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bioRxiv - Cell Biology 2022Quote: ... These proteins were expressed in One Shot™ BL21 (DE3) competent cells (Invitrogen).
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bioRxiv - Biochemistry 2022Quote: ... the protein was overexpressed in One Shot BL21(DE3)pLysS E.coli cells (Invitrogen) in LB media at 37 °C for 8 h ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were then pre-cleared by addition of Dynabeads Protein G (ThermoFisher) and rotated at 4°C for 3 h ...
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bioRxiv - Immunology 2020Quote: ... FreeStyle™ 293F cells for protein production were obtained from Life Technologies (#R79007) and cultured in FreeStyle™ 293 Expression Medium (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... The protein was expressed in FreeStyle 293-F cells (ThermoFisher Scientific; Cat# R79007) and purified by immuno-affinity purification using 2G12 mAb as previously described 7 ...
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bioRxiv - Microbiology 2021Quote: Cells were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific). Protein lysates were run on 4%–12% Bis-Tris gels (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: Cell Stimulation Cocktail (plus protein transport inhibitors) (500X) (Thermo Fisher, #00-4975-93) was diluted in T cell medium (RPMI 1640 + 10%FBS + L-glutamine (2 mM ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts were quantified using Pierce BCA Protein Assay Kit (Thermo Scientific; PIER23225) and 1mg was incubated with 40µl of Flag-M2 Affinity Agarose Gel (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... These proteins were expressed in BL21 Star (DE3) Escherichia coli cells (Life Technologies). The expression and purifications were either in LB or M9 minimal media supplemented with 15NH4Cl with slight modifications as previously described (Conicella et al. ...
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bioRxiv - Systems Biology 2020Quote: ... PM proteins were extracted using a cell surface isolation kit (Thermo Fisher Scientific), whereas mitochondrial proteins were isolated using the Mitochondria Isolation Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins cloned in pTYB vectors were expressed in BL21(DE3) cells (Invitrogen), grown in Terrific Broth medium at 37 °C until the OD600 reached a value of 1.5-2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were subsequently prepared by lysing cells in RIPA buffer (Life technologies) supplemented with protease and phosphatase inhibitor cocktails (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: Cas9 protein variants were expressed in BL21 star (DE3) cells (Thermo Fisher Scientific) using a previously established protocol with minor modifications (Jinek et al. ...
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bioRxiv - Neuroscience 2019Quote: ... All proteins were expressed in Human Embryonic Kidney (HEK) 293 Freestyle cells (Invitrogen) in suspension culture using serum-free media ...
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bioRxiv - Immunology 2020Quote: ... cell protein lysates were harvested using a lysis and extraction kit (ThermoFisher Scientific) supplemented with Halt Protease & Phosphatase Inhibitor Cocktail (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: This recombinant S protein was overexpressed using the HEK 293F mammalian cells (Invitrogen) at 37°C under 5% CO2 in a Multitron-Pro shaker (Infors ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Dimeric sACE22-IgG1 proteins were expressed in ExpiCHO-S cells (Thermo Fisher Scientific) cultured in ExpiCHO Expression Medium (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: Tissue and whole cell lysates were prepared in Protein Extraction Reagent (Thermo Fisher) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... Cells were lysed in M-PER® Mammalian Protein Extraction Reagent (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Cell lysates were generated with M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: All proteins were transiently transfected in suspension HEK293F cells (Invitrogen, cat no. R79009) maintained in Freestyle medium (Life Technologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were disrupted using B-PER Bacterial Protein Extraction Reagent (ThermoFisher cat#78248) containing 6 mg/ml lysozyme ...
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bioRxiv - Biochemistry 2022Quote: All recombinant proteins were produced in Rosetta2 Escherichia coli competent cells (Thermo Fisher) induced with 0.4 mM isopropyl-β-d-1-thiogalactoside (IPTG ...
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bioRxiv - Cancer Biology 2022Quote: ... B16F10 cells were transfected with labeled sgRNAs and Cas9 protein (Thermo Fisher Scientific) using CRISPRMax Cas9 transfection reagent (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lysates were pre-cleared with 30 μl protein G beads (Life Technologies), and then incubated with 2 μg isotype matched IgG control or indicated antibodies on a rocking platform overnight at 4℃ ...
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bioRxiv - Physiology 2022Quote: Protein was extracted from cells and whole kidneys using RIPA buffer (Thermo Scientific) with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Immunology 2023Quote: ... The cell-free supernatant was incubated overnight with either protein A resin (ThermoFisher) for IgG1 or LambdaFabSelect or KappSelect resin (Cytvia ...
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bioRxiv - Neuroscience 2023Quote: ... protein lysates were derived from cell cultures using RIPA buffer (#89900, Thermo Scientific) containing both protease and phosphatase inhibitors (#11836170001 ...
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bioRxiv - Biochemistry 2023Quote: Sf9 cells used for expressing proteins with baculovirus system were procured from Gibco™ Sf9 cells in Sf-900™ III SFM ...
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bioRxiv - Immunology 2023Quote: ... RBD proteins were expressed in-house in Expi293F human cells (Thermo Fisher Scientific) by transfection of the cells with purified DNA and polyethylenimine (PEI) ...
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bioRxiv - Biophysics 2022Quote: ... was used to conduct protein expression in HEK 293-F cells (Gibco, USA) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were produced in suspension culture in Freestyle 293F cells (Life Technologies) transiently transfected using FectroPro reagent (Polyplus) ...
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bioRxiv - Physiology 2023Quote: ... and cells were lysed in mammalian protein extraction reagent (M-PER; Thermo Fisher) containing protease inhibitors cocktail (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were expressed in SF9 or High Five™ Cells (Thermo Fisher) (BTI-TN-5B1-4 ...