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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cell passaging was performed every 3 to 4 days using 0.05% Trypsin-EDTA solution (Gibco). Expi293F cells were maintained in Expi293 Expression Medium (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... Cells were passaged every 2-3 days by detachment with 0.05% trypsin-EDTA (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... Cells were maintained from DIV0-3 in an induction medium consisting of DMEM-F12 (Gibco), 1x N2 (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... The fixed cells are blocked with 3% normal goat serum (NGS) (Thermofisher Scientific, Cat# 31872) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Red blood cells were lysed with ACK Lysing Buffer (Gibco, 3 ml for 5 min). The cells were counted and resuspended in RPMI-1640 medium (Corning ...
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bioRxiv - Molecular Biology 2024Quote: ... passage 2-3 NPCs were detached with Stempro Accutase cell dissociation reagent (A1110501, Life Technologies) and kept in cold PBS 1%FBS for the duration of the protocol ...
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bioRxiv - Molecular Biology 2019Quote: Relative transcript abundance of HarmGr9 in control and Gr9 silenced insects was determined by quantitative Real-Time PCR (qRT-PCR) using 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster, CA, USA) and GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... The wall was coated with Insect-a-Slip (Bioquip Products, Inc., cat# 2871C) and the ceiling was coated with Surfasil Siliconizing Fluid (ThermoFisher Scientific, cat# TS-42800), to prevent flies from climbing as described previously (Asahina et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Each circular arena measured 16 mm diameter x 12 mm high and was coated with Insect-a-Slip (Bioquip Products, Rancho Dominguez, CA) on the walls and SurfaSil Siliconizing Fluid (Thermo Fisher Scientific, Waltham, MA) on the clear acrylic top plate to confine the flies to the bottom plate ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed with PBS and fractionated using a subcellular protein fractionation kit for cultured cells (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Approximately 2 × 106 cells were fractionated using the Subcellular Protein Fractionation Kit for Cultured Cells (Thermo scientific, #78840).
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bioRxiv - Cancer Biology 2023Quote: Proteins were extracted from shβ7 and pTer1 cells using Cell Disruption Buffer (miRVana™ Paris™ kit, Thermofisher) and were quantified with microBCA protein Assay kit (Thermofisher ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 3 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days at which point the supernatant was harvested ...
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bioRxiv - Biochemistry 2020Quote: ... Anti-phospho SMAD2/3 (8828S, Cell Signalling Technology) and anti-SMAD2 (5339S, Cell Signalling Technology) were diluted 1000X and anti-GAPDH (AM4300, Thermo Fisher) was diluted 5000X in PBST-T supplemented with 3 % bovine serum albumin ...
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bioRxiv - Genetics 2021Quote: The human lung epithelial cell line Calu-3 (UC Berkeley Cell Culture Facility) was maintained RPMI media supplemented with Glutamax (Thermo Fisher), 10% FBS (HyClone) ...
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bioRxiv - Neuroscience 2020Quote: ... These cells were differentiated by plating at a density of 3×105 cells/well in 96-well plates (Nunc, Thermofisher, UK) in Dulbecco’s modified Eagle’s medium (0,1% horse serum supplemented with nerve growth factor (50 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... by gently washing the cells in PBS then the remaining cells were fixed (Hema 3 fixative) and stained using a Geimsa-Wright stain (Fisher scientific). The infection and staining technique have been previously described (Rajan et al. ...
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bioRxiv - Microbiology 2021Quote: ... 16.5 µl of cell suspension were used to recover 10,000 cells and libraries were quantified with a Qubit 3 Fluorometer (Thermo Fisher Scientific). Quality was checked using a 2100 Bioanalyzer with High Sensitivity DNA kit (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... suspension-adapted and serum-free HEK 293S cells were seeded at 3 x 106 cells/mL in Freestyle-293 medium (ThermoFisher Scientific). Next ...
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bioRxiv - Microbiology 2021Quote: Suspensions of Vero E6 and Calu-3 cells were stained with LIVE/DEAD Fixable Aqua Dead Cell Stain kit (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Cells grown to a density of 3 million cells per milliliter were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and grown for 4 days ...
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bioRxiv - Immunology 2019Quote: ... The CD4+ T cells were activated for 3 days using anti-CD3/anti-CD28 T-cell activation and expansion beads (Dynabeads, ThermoFisher Scientific) in complete medium (RPMI 1640 media supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Cell Biology 2019Quote: JEG-3 trophoblast cells were purchased from Cell Bank of Chinese Academy of Sciences and were cultured in MEM medium (Gibco, 10370021) with the addition of 0.11g/L Sodium Pyruvate (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... were cultured in DMEM-F12 containing PS and 5% FBS for CHO cells or 3% FBS for CHO-ldlD cells and transfected using Lipofectamine 2000 (Thermo Fisher) following standard protocol ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 3 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for 3-5 days ...
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bioRxiv - Cell Biology 2020Quote: ... Fresh human NK cells were cultured for 3 days at a density of 1×106 cells/ml in RPMI 1640 medium (Gibco, Thermo Fisher) containing 10 ng/ml recombinant human IL-2 (Gibco ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 3 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then diluted 1:1 with Trypan Blue stain and the number of cells stained were counted using a Countess™ 3 Automated Cell Counter (ThermoFisher).
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bioRxiv - Cancer Biology 2022Quote: ... cell density was adjusted to 106 cells/mL (and was never allowed to go over 3×106 cells/mL) and puromycin selection (2 μg/mL, Gibco, A1113803) was started ...
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bioRxiv - Genomics 2022Quote: The SARGENT library and piggyBac transposase were co-transfected into K562 (LP cell lines) cells at a 3:1 ratio using the Neon Transfection System (Life Technologies). For each experiment ...
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bioRxiv - Immunology 2022Quote: ... Cells grown to a density of 3 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days prior to supernatent harvest ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was removed from cell pellets and fixed cells were then resuspended in FACS buffer (PBS [Gibco] supplemented with 3% FBS). Flow cytometry was performed on the Stratedigm S1000EON benchtop flow cytometer ...
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bioRxiv - Biochemistry 2023Quote: ... Cells grown to a density of 3 million cells per mL were transfected with the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days at which point the supernatant was harvested ...
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bioRxiv - Immunology 2023Quote: ... cells were grown to a density of 3 × 106 cells/mL and transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific). Three to 5 days post-transfection ...
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bioRxiv - Physiology 2023Quote: ... and differential cell counts were performed after cytocentrifuging cells on glass slides and staining with HEMA 3 (Fisher Scientific, Tustin, CA). Total protein levels were determined as previously described 22 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell count and proportion of dead cells were quantified for ≥ 3 technical replicates using a Countess II FL Automated Cell Counter (ThermoFisher AMQAf1000). The cell count for doxycycline induction was normalized to the without-doxycycline condition for each of three biological replicates per condition ...
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bioRxiv - Cell Biology 2024Quote: ... was transfected into AtT-20 cells (2.0× 105 cells/well) using 12-well plates and 3 μL of the Lipofectamine RNAiMAX reagent (Thermo Fisher Scientific) by reverse transfection ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting pellet was resuspended in DMEM/F-12 and cell density was determined using the Countess 3 Automated Cell Counter (Invitrogen, AMQAF2000) using Trypan blue solution (Gibco ...
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bioRxiv - Immunology 2024Quote: ... at a 3:1 bead-to-cell ratio in T-cell media consisting of OpTmizer CTS SFM media (Thermo Fisher Scientific) supplemented with 5% human AB serum and 100 Units/mL human IL-2 (PeproTech) ...
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bioRxiv - Bioengineering 2024Quote: ... Cell count and viability was assessed using trypan blue dye exclusion with a Countess 3 automated cell counter (Thermo Fisher Scientific), and the cell concentration was adjusted to approximately 1000 cells/uL ...
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bioRxiv - Bioengineering 2020Quote: ... Intensity quantification was performed using ImageJ software and the obtained values were corrected by culture media background (without having been in contact with cells) and total protein content measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). Values were further normalized by internal negative and positive controls from the antibody assay ...
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bioRxiv - Genetics 2022Quote: Total protein was isolated from kidneys or cells using lysis buffer made from mixing T-PER tissue protein extraction reagent (Invitrogen, catalog# 78510) with a protease phosphatase inhibitor tablet (Fisher ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations of the tissue homogenates or whole-cell extracts were determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... 5 mM MgCl2) for cell membrane binding proteins (LPL, Caveolin 1) and quantified with Pierce™ BCA Protein Assay Kit (Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Mitochondria enriched fractions were processed similar to whole cell samples except they were normalized via protein concentration as determined by the Pierce™ BCA Protein Assay kit (Thermo Scientific). In Brief ...
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bioRxiv - Neuroscience 2022Quote: ... Plates were read using an MSD SECTOR Imager 2400 and resulting peptide concentrations were normalized to total protein in the cell lysate per well measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Data for each shRNA knockdown were additionally normalized to the average of control conditions for each parameter measured.
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bioRxiv - Physiology 2023Quote: ... BAL cells were counted using a LUNA-II automated cell counter (Logos Biosystems) and BAL supernatant total protein was measured using a Pierce total protein assay (Thermo Scientific, #23225).
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bioRxiv - Immunology 2023Quote: Supernatants were collected from cell cultures and protein extracts of lung tissue were obtained using T-PER Tissue Protein Extraction Reagent (ThermoFisher, Cat# 78510). The levels of mouse IL-5 and IL-13 were measured with DuoSet ELISA kits (DY405 and DY413 respectively ...
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bioRxiv - Neuroscience 2023Quote: ... was recombinantly expressed as an N-terminal Fc-fusion protein (Fc-hTfR1) in Expi293 suspension cells and purified by gravity flow with PierceTM Protein A Agarose (Thermo Fisher Scientific). HFCII probes were loaded with Fc-hTfR1 by thresholding to a 1 nm shift ...
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bioRxiv - Cell Biology 2024Quote: ... Following treatment cells were then transduced with Premo™ Autophagy tandem sensor Red Fluorescent Protein-Green Fluorescent Protein (RFP-GFP)-LC3B (Life Technologies, Invitrogen) and chloroquine was used as control ...