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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Cell Biology 2019Quote: ... and 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) (Invitrogen) in water for 1 hour at 60°C ...
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bioRxiv - Neuroscience 2019Quote: ... Fluo-3-acetoxymethylester (Fluo-3-AM) (Molecular Probes-Thermo Fisher Scientific) was used (Beauvais et al. ...
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bioRxiv - Neuroscience 2019Quote: ... Fluo-3-acetoxymethylester (Fluo-3-AM) (Molecular Probes-Thermo Fisher Scientific) was used (Beauvais et al. ...
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bioRxiv - Biophysics 2022Quote: ... and 2 ng/mL recombinant murine interleukin-3 (IL-3) (Gibco). Cells were grown in a humidified incubator at 37 °C and 6% atmospheric CO2 ...
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bioRxiv - Biophysics 2022Quote: ... 19 mg EDC (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (Thermo Fisher), 11 mg sulfo-NHS (N-Hydroxysulfosuccinimide ...
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bioRxiv - Immunology 2022Quote: ... 1.9g of ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) (ThermoFisher #22980) and 1.2g of N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2023Quote: ... 1 % dipalmitoyl PI(3)phosphate (PI(3)P diC16) (Life Technologies) and extruded to 400 nm using a polycarbonate filter and a hand extruder (Avanti Polar Lipids ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was performed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Microbiology 2020Quote: Concentrated virus samples were mixed with Novex™ Tris-Glycine SDS Sample Buffer (2X) (Thermo Fisher Scientific) with NuPAGE™ Sample Reducing Agent (10X ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion, Life Technologies, Carlsbad, CA) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion, Life Technologies, Carlsbad, CA) and a mixture of anchored oligo-dT and random decamers (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... the virus solution was typically mixed at 1:500 with blue beads (fluorescent polymer microspheres, Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... Reovirus S4 RNA was quantified using the Taq-Man Fast Virus One-Step Master Mix (Applied Biosystems) and GAPDH was detected as an endogenous control using the Pre-Developed TaqMan Assay Reagent kit for mouse GAPDH (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... reverse transcription was performed with random hexamers and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen). Synthesized RNA was used as a standard (BEI) ...
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bioRxiv - Microbiology 2021Quote: The spike of the SARS-CoV-2 virus (D614G variant) was generated (GeneArt Gene Synthesis, ThermoFisher Scientific) from the codon-optimized sequence obtained by Ou et al ...
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bioRxiv - Microbiology 2020Quote: ... centrifuged and suspended in virus media that is composed of DMEM high glucose (Thermo Fisher Scientific, USA), with 2% fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Master mix for qRT-PCR was prepared using TaqMan Fast Virus 1-step Master Mix (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from 1010 virus particles using the PureLink TM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were generated by Sendai virus reprogramming and clonal expansion using the CytoTune – iPSC 2.0 kit (ThermoFisher) to deliver the reprogramming factors ...
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Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms in neuronal cellsbioRxiv - Cell Biology 2022Quote: Each RNA sample was subjected to reverse transcription using murine leukemia virus reverse transcriptase (Thermo Fisher Scientific), and the generated cDNA was used as a template for qRT-PCR ...
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bioRxiv - Immunology 2021Quote: ... Reactions were performed using TaqMan® Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, Waltham, USA) and 5 μl of isolated RNA as a template ...
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bioRxiv - Microbiology 2020Quote: ... Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher) and a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Microbiology 2021Quote: Virus stock were diluted in 10-fold serial dilutions in 2ml Opti-MEM I (1X) + GlutaMAX (Gibco). 1 ml of each dilution was added to monolayers of 2 × 106 VeroE6 cells in the same medium in a 6-well plate ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNAs were synthesized using Moloney murine leukemia virus RT with random primers (Invitrogen, Thermo Fisher Sci.). RNA levels of representative transcripts were analyzed in triplicate by quantitative PCR with SYBR™ Green using a Bio-Rad CFX thermal cycler ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNAs were synthesized using Moloney murine leukemia virus RT with random primers (Invitrogen, Thermo Fisher Sci.). RNA levels of representative transcripts were analyzed in triplicate by quantitative PCR with SYBR™ Green using a Bio-Rad CFX thermal cycler ...
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bioRxiv - Immunology 2021Quote: ... Reactions were conducted with 5 μL RNA and TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems) with 500 nM primers and 200 nM probes ...
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bioRxiv - Neuroscience 2021Quote: ... The genomic titer of each virus was determined by quantitative PCR using the ABI 7700 (Applied Biosystems) and primers specific to the WPRE ...
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bioRxiv - Immunology 2020Quote: ... Virus detection was performed using One-Step RT-PCR SuperScript(tm) III Platinum(tm) Kit (Life Technologies). Thermal cycling was achieved at 55°C for 10 minutes for reverse transcription ...
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bioRxiv - Microbiology 2022Quote: ... and plasmids encoding two vaccinia virus capping enzyme subunits (pCAG-D1R and pCAG-D12L −0.8μg each) using 16μL Lipofectamine 2000 (Invitrogen) per transfection reaction in a total volume of 200μL of Opti-MEM (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... Real-time RT-PCR analysis was performed using Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... iPSC lines were generated using the Sendai virus (CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific) carrying the Yamanaka reprogramming factors OCT3/4 ...
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bioRxiv - Neuroscience 2023Quote: ... medium containing virus was processed by centrifugation (Sorvall WX-90 Ultracentrifuge and SureSpin 630 rotor; ThermoFisher Scientific), using a sorbitol cushion (20% sorbitol ...
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bioRxiv - Neuroscience 2023Quote: Expanded PBMCs were transduced Sendai virus reprogramming vector – according to CytoTune® 2.0 Sendai (Thermo Fisher Scientific). Before cells infection ...
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bioRxiv - Molecular Biology 2023Quote: ... purified gRNA from benzonase-treated virus material was treated with DNaseI (Thermo Fisher Scientific, Waltham, MA, USA), and analyzed using an Agilent 2100 bioanalyzer (Agilent Technologies ...
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bioRxiv - Genetics 2023Quote: ... The PBMCs were reprogrammed by using Sendai virus (CytoTuneTM-iPS 2.0 Sendai Reprogramming kit, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The following primary antibodies were used: influenza A virus nucleoprotein (NP, PA5-32242, 1:3000, ThermoFisher Scientific), XPO1 (46249S ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher) and a LightCycler 480 or LC96 instrument (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Presence of each virus strain in the supernatant was determined by PCR using Taq polymerase (Thermo Scientific) and 20 μL reactions containing 1 μL of virus supernatant ...
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bioRxiv - Bioengineering 2023Quote: ... reverse transcription was performed with random hexamers and Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Invitrogen). Synthesized RNA was used as a standard (BEI) ...
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bioRxiv - Biochemistry 2023Quote: ... virus was harvested by collecting supernatant and filtering it through 0.45µm PES sterile filter (Thermo Fisher, 2954545). Virus was concentrated by ultracentrifugation with a 20% sucrose cushion ...
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bioRxiv - Cell Biology 2023Quote: ... from a cDNA library of HCT116 which was prepared using Moloney murine leukemia virus reverse transcriptase (Invitrogen), as described previously (Ninagawa et al. ...
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bioRxiv - Microbiology 2023Quote: ... The BA.1 and BA.2 supernatants were additionally concentrated using the Intact Virus Precipitation Reagent (Invitrogen). All viral stocks were stored at -80°C until use ...
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bioRxiv - Microbiology 2024Quote: ... and the virus was incubated with 25 μg of Alexa Fluor 488 NHS Ester (succinimidyl ester; Invitrogen) for 1 hour at 25°C ...
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bioRxiv - Microbiology 2024Quote: ... half of the virus supernatant after the concentration step was digested with amplification grade DNAse I (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from 1010 virus particles using the PureLink TM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed in B-PER bacterial protein extraction reagent (Thermo Scientific, MA) containing 2500 U of DNAseI (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell protein lysates were mixed with NuPAGE LDS Sample Buffer (4X) (NP0007; Invitrogen) and run on polyacrylamide gels ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with siRNA control or siRNA against specific proteins (Life Technologies). DharmaFECT transfection reagent (0.1 μL per well ...