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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Protein extracts were resolved using either home-made SDS-PAGE gels or 3-8% NuPAGE Tris-Acetate gels (Thermo Fisher Scientific), when analysing proteins of a molecular weight >180 kDa ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
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bioRxiv - Biochemistry 2022Quote: ... the protein solution was dialyzed in buffer A for 3 h and passed through HisPur Ni-NTA resin (Thermo Fisher Scientific) to remove the 6×His tag ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: The lysate and purified proteins were incubated with 3 µg of biotinylated RNA probe coupled to 20 µl Dynabeads MyOne Streptavidin C1 (Thermo Fisher) in 600 µl lysis buffer at 4°C for 1 hour rotating head-over-tail ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Neuroscience 2021Quote: Flash frozen samples (n=3 per genotype) were prepared for Western blot using the Mem-PER Plus protein extraction kit (89842, Thermo Scientific) to isolate membrane and cytoplasmic protein fractions from mice aged P29-32 ...
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bioRxiv - Cell Biology 2022Quote: ... was added to the supernatant and 100 μg of protein was loaded onto a 3-12% native gel (NativePAGE™ (Invitrogen)) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were run on a commercial native- PAGE gel (3 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well, Invitrogen, Cat. BN1001BOX) at 100 V tension using as running buffer 50 mM BisTris ...
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bioRxiv - Systems Biology 2021Quote: ... coli phosphate-binding protein labeled with 7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC) (phosphate sensor, CAT # PV4406, Thermo Fisher) upon binding of the free phosphate GTP hydrolysis product (excitation at 425 nm ...
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bioRxiv - Immunology 2020Quote: Aag2 cells were seeded at 1 × 106 cells/well in 12-well plates and transfected at the time of seeding with 3 μg protein expression plasmid using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... For the detection of Gli3 10μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies (3 μg for ChIP-qPCR and 9 μg for ChIP-seq) were coupled to protein G dynabeads (Thermo Fisher Scientific) for 6 hours at 4°C and then incubated with fragmented chromatin over night at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting membrane pellet was resuspended in buffer M10 at 3 mL/g (of starting mitochondria) and the protein concentration was determined using a BCA assay (Pierce Thermo Fisher). The resuspended membranes were stored at 10 mg/mL of total protein at -80°C in a final glycerol concentration of 30% (v/v ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Molecular Biology 2022Quote: ... Fractions with the greatest quantity and purity of UBC9 protein combined and concentrated down to 5 ml using 3-kDa MWCO centrifugal concentrator (Thermo Scientific) at 4,000xg/4 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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bioRxiv - Cancer Biology 2024Quote: Crude protein extraction obtained as described above was electrophoresed through a slab isoelectric focusing gel (pH 3-7, Invitrogen Novex EC66452) employing freshly made cathode and anode buffers (Novex) ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... was used to quantify protein concentration and protein samples were diluted to 3 μg/μL in Laemmli SDS sample buffer (catalog number J61337.AC, Thermo Fisher) and boiled at 100°C for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... of the homogenized protein-loading buffer sample were loaded in duplicates on a NuPAGE™ 3-8% Tris-Acetate gel (ThermoFisher) to be probed separately with FLAG and ATPase antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were separated by magnet and washed with IP lysis buffer 3 times before proteins were eluted by heating in 2xNuPAGE LDS sample buffer (Invitrogen, NP0008) and 2x sample reducing buffer (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... The barrier function of the Calu-3 monolayer was terminally evaluated via immunofluorescence imaging of tight junction protein 1 (ZO-1 antibody, Invitrogen, 339194) and permeability assays using 500 µL of 2 mg/mL FITC-dextran (4 kDa ...
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bioRxiv - Microbiology 2023Quote: ... the indicated amounts of protein sample were boiled at 95°C for 3 min and separated by 4–12% Bis-Tris SDS-PAGE (Thermo Scientific) either in 1x MES buffer (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: Fluorescent labelling of Tau and 14-3-3ζ protein for microscopy assays was done using amine-reactive DyLight405 or DyLight488-NHS ester (Thermo Scientific) following the manufacturer instructions and the established protocol 34.
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bioRxiv - Biochemistry 2023Quote: ... Raw data files were searched against the amino acid sequences of mCherry-ATG14 Class III phosphatidylinositol 3-kinase complex I (mCherry-PI3KC3-C1) proteins using Proteome Discoverer software (version 1.3, SEQUEST algorithm, Thermo Fisher Scientific) for tryptic peptides (i.e. ...
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bioRxiv - Cell Biology 2023Quote: ... to a final detergent:dye ratio of 16 g/g before running on a 3-12% NativePAGE Mini Protein Gel (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and equal volumes containing equal total protein were loaded into wells of precast NativePAGE Novex® 3%–12% Bis-Tris 10-well Gels (Invitrogen). High molecular weight (HMW ...
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bioRxiv - Plant Biology 2024Quote: ... Denatured samples were resolved by SDS-PAGE as described and native protein extracts were resolved in precast NativePAGE™ Novex 3–12% Bis-Tris Gels (Invitrogen) alongside 10 μl of Serva Native Marker ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed 3 times in 5% Bovine Serum Albumin (BSA; ThermoFisher Scientific, 11423164) in PBS then incubated with primary antibody to H2AX (Ser139) ...
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bioRxiv - Genetics 2021Quote: ... Calu-3 cells were maintained in MEM α media supplied with 10% FBS (Gibco) and 1% Pen/Strep (0.025% ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were split every 3 days at 1:10 using Trypsin-EDTA (ThermoFisher Scientific). On day 1 ...
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bioRxiv - Cell Biology 2022Quote: PC-3 cells (ECACC 90112714) were maintained in RPMI medium (GIBCO, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: PC-3 cells (ECACC 90112714) were maintained in RPMI medium (GIBCO, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... The Calu-3 cells were cultured with MEM (Thermo Fisher Scientific, Waltham, MA, USA) containing 20% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were fixed and stained using the Protocol Hema 3 staining kit (Fisher Scientific). Colony number and size were determined using the Fiji Is Just ImageJ (Fiji ...
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bioRxiv - Microbiology 2019Quote: Calu-3 epithelial cells (ATCC HTB-55) were maintained in optiMEM medium (Life Technologies) supplemented with 5% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged every 2-3 days by washing with HBSS (Gibco, cat#14170), trypsinizing using 0.25% Trypsin-EDTA (Gibco ...
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bioRxiv - Physiology 2021Quote: ... cells were fed every 2-3 days with DMEM + 10% FBS (Thermo fisher Scientific) until differentiation was complete at day 10.
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with 3 mM dimethyl 3,3′-dithiobispropioimidate (DTBP; Thermo Fisher Scientific, #20665) for 5 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... HCT8 cells were grown on a 6-well slide (3×105/well, Thermo Scientific) overnight ...
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bioRxiv - Cancer Biology 2020Quote: HEK293T cells (3×105) were seeded in a 6 well plate (Nunc,Thermo,USA) with DMEM media (Invitrogen,USA ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 3 times with PBS and mounted with Prolong Gold (Molecular Probes) containing 1 μg/ml DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... Calu-3 cells purchased from ATCC (Cat# HTB-55) were maintained in MEM (Invitrogen), containing 10% (v/v ...
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bioRxiv - Cell Biology 2022Quote: MUTZ-3 cells (DSMZ) were cultured at 37°C in alpha-MEM (Life Technologies) supplemented with 20% FBS ...
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bioRxiv - Bioengineering 2022Quote: Cell apoptosis was evaluated with CellEvent® Caspase 3/7 Green (Thermo Fisher, UK), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Cells were selected 3 days after transfection with puromycin (2µg/mL) (Thermo Fisher Scientific) for a duration of 5 days ...