Labshake search
Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: The cell membrane protein samples were collected using Mem-PER eukaryotic membrane protein extraction reagent kit (Thermo Scientific, 89826, MA) according to the protocol instruction ...
-
bioRxiv - Cancer Biology 2021Quote: Subcellular protein fractionation was performed either by using Subcellular Protein Fractionation Kit for Cultured Cells (Thermo Scientific; Cat. no. 78840) according to the manufacturer protocol or following the protocol below.
-
bioRxiv - Cell Biology 2019Quote: Cells were lyzed using RIPA buffer and proteins were quantified using the BCA method (Protein BCA Assay Kit, Thermo Fisher). The protein extracts were separeted on 12% SDS-PAGE gels (50 μg of total proteins were loaded per well ...
-
bioRxiv - Developmental Biology 2020Quote: Protein in testes and cultured cells were extracted using T-PER Tissue protein extraction reagent (Thermo Fisher Scientific, Waltham, Massachusetts), subjected to SDS-PAGE ...
-
bioRxiv - Developmental Biology 2020Quote: ... cells were pelleted and 50μg of protein was quantified by ionic detergent compatible Pierce 660nm Protein Assay Reagent (Thermo Fisher) in 8M Urea ...
-
bioRxiv - Biochemistry 2021Quote: Protein concentration of cell lysates was determined using the PierceTM BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were adjusted in 5xLDS sample buffer containing 50 mM DTT ...
-
bioRxiv - Cancer Biology 2020Quote: ... Supernatant of cell lysates was assayed for total protein content using the BCA protein assay (Thermo Fisher Scientific, Rockford, IL), and 70 μg of total protein per sample was separated in a 7.5% polyacrylamide gel (Invitrogen) ...
-
bioRxiv - Cell Biology 2022Quote: ... was used to lyse cell pellets and protein concentration determined using the Micro BCA Protein Assay kit (ThermoFisher Scientific, 232350). 10 μg protein ...
-
bioRxiv - Physiology 2022Quote: Protein concentration of cell lysates was determined using the PierceTM BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were subsequently subjected to SDS-PAGE and blotted on a nitrocellulose membrane ...
-
bioRxiv - Bioengineering 2022Quote: ... Extracted protein from the cell-only control samples was quantified using Pierce Rapid Gold BCA Protein Assay Kit (Thermo Fisher). 10 µg protein from each sample was then analyzed by sodium dodecyl sulfate– polyacrylamide gel electrophoresis (SDS-PAGE ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cell debris were pelleted by centrifugation (14,000g, 10min, 4°C) and whole cell protein extract concentration was measured using Qubit Protein Assay Kit (Thermo Fisher). Protein extracts were immediately used for EnzyChrom Catalase Assay Kit and EnzyChrom Glutathione Reductase Assay Kit (BioAssay Systems) ...
-
bioRxiv - Cell Biology 2021Quote: ... The protein concentration of clarified cell lysate was then measured using the BCA protein assay kit (Thermo Fisher, Cat# 23227). 5 ∼ 20 μg of total protein per lane was separated by SDS-PAGE gel and transferred to PVDF membrane ...
-
bioRxiv - Neuroscience 2019Quote: Whole cell protein lysates were isolated from hippocampal tissues by using M-PER mammalian protein extraction reagent (Thermo Scientific, 78501) and by adding Halt Protease Inhibitor (Thermo Scientific ...
-
bioRxiv - Biochemistry 2019Quote: ... Cell-surface proteins were purified using Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, Rockford, lL, USA). Isolated proteins were labeled with Cy3 dye and hybridized to a lectin microarray 27–35 ...
-
bioRxiv - Molecular Biology 2019Quote: The protein lysate or exosomal protein from of WT H69 and ∆huPGRN-H69 cells were prepared using M-PER mammalian protein extraction reagent (ThermoFisher) or exosome protein isolation kit (ThermoFisher ...
-
bioRxiv - Molecular Biology 2021Quote: ... 60 μg of proteins in cell lysates were separated on a NuPAGE™ 4-12% Bis-Tris Protein Gel (Invitrogen) and transferred onto a nitrocellulose membrane (GE ...
-
bioRxiv - Microbiology 2021Quote: ... cytoplasmic proteins and the total cell lysate proteins were denatured and boiled with 4× LDS sample buffer (Cat. #NP0007, Invitrogen) and subjected to immunoblotting analysis with equal protein loading of extracts (~20 µg/lane) ...
-
bioRxiv - Microbiology 2021Quote: ... total cell protein was extracted and subjected to co-immunopreciptation (Co-IP) with Dynabeads-Protein G (Thermo Fisher Scientific, USA) followed by the protocol described previously (12).
-
bioRxiv - Cancer Biology 2022Quote: ... cells were lysed with 15 μl RIPA buffer and protein concentration was quantified using BCA Protein Assay Kit (Thermo Scientific). OCR measurements were normalized to the protein concentration in each well.
-
bioRxiv - Microbiology 2023Quote: ... The protein concentration of the cell lysate was measured by BCA assay (Pierce BCA Protein Assay Kit, Thermo Scientific, USA). The cell pellet was resuspended in 40 μl ddH2O and mixed carefully ...
-
bioRxiv - Cell Biology 2023Quote: ... The protein concentrations of the both the tissue and cell lysates were assessed by BCA Protein Assay Kit (ThermoFisher #23227) and protein samples were supplemented with 4x laemmli blue (Bio-Rad #1610747 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Total protein concentration of the cell lysates was quantified using the Pierce BCA Protein Assay Kit (ThermoFisher, Cat. No. 23227). Cell lysates were mixed with the sample buffer (with beta mercaptanol ...
-
bioRxiv - Plant Biology 2023Quote: ... Surface-exposed proteins were biotinylated and purified using the Pierce™ Cell Surface Protein Biotinylation and Isolation Kit (ThermoFisher Scientific), following the manufacturer’s protocol with some modifications ...
-
bioRxiv - Developmental Biology 2023Quote: ... The total protein concentration in the cell lysate was quantified by using BCA protein Assay kit (Thermo Scientific™, 23225).
-
bioRxiv - Cell Biology 2023Quote: ... the protein concentration of the cell lysates was measured using a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific). Equal amounts of total protein (1 mg ...
-
bioRxiv - Developmental Biology 2023Quote: Membrane proteins were extracted from cell pellets using the Mem-PER™ Plus Membrane Protein Extraction Kit (89842, Thermo Fisher) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: The protein concentration of the cell lysates was determined with the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For co-immunoprecipitation ...
-
bioRxiv - Microbiology 2022Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
-
bioRxiv - Pathology 2023Quote: ... and the number of 4T1 cells was counted using an automated cell counter (Countess™ 3 FL Automated Cell Counter, Thermo Fisher Scientific). A cell resuspension with a final cell density of 1 × 106 cells/ml was prepared in PBS.
-
bioRxiv - Microbiology 2023Quote: ... RAW264.7 cells passaging was achieved by gentle cell scraping and seeding cells at a density of 3×106 cells/T75 flask (Nunc; Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2020Quote: ... the membranes were washed again 3 times for 10 minutes with 1x TBST and proteins were visualized using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific). Signals were digitally captured on a Fusion Solo S (Witec ...
-
bioRxiv - Genetics 2021Quote: ... Equal amounts of protein (in vitro samples: 3 μg; in vivo samples: 80 μg) were separated by SDS-PAGE (Thermo Fisher) and transferred to a 0.45 μm nitrocellulose membrane (Amersham) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 10-20 µg of protein loaded on a 3%–8% NuPAGE Tris-Acetate Gel in tris-acetate buffer (Novex Life Technologies) or a 4-12% NuPAGE TrisBis NuPAGE gels in MES buffer (Novex Life Technologies ...
-
bioRxiv - Molecular Biology 2020Quote: ... The dried peptides were resuspended in 0.1% FA and 3% ACN in water and protein quantity measured at A280 via NanoDrop (Thermo Fisher Scientific). Concentrations were normalized across all fractions and iRT (Biognosys ...
-
bioRxiv - Molecular Biology 2019Quote: 150 µl of cross-linked sample (∼3 mg of total protein) were digested by SMART Digest Trypsin Kit (Thermo Fisher Scientific), by adding 450 µL of SMART Digest buffer containing 50 µg of beads at 70°C for 4 h ...
-
bioRxiv - Biochemistry 2020Quote: ... 10 μg protein/lane was then separated under denaturing conditions on reducing 3~8% Tris-Acetate gels (NuPAGE, Thermo Fisher Scientific), and HiMark protein standard (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... 3-5 µg of protein was subjected to nanoflow liquid chromatography on an EASY-nLC system (Thermo Fisher Scientific, Bremen, Germany) on-line coupled to an Q Exactive HF quadrupole orbitrap mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
-
bioRxiv - Molecular Biology 2022Quote: Protein extracts were resolved using either home-made SDS-PAGE gels or 3-8% NuPAGE Tris-Acetate gels (Thermo Fisher Scientific), when analysing proteins of a molecular weight >180 kDa ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
-
bioRxiv - Biochemistry 2022Quote: ... the protein solution was dialyzed in buffer A for 3 h and passed through HisPur Ni-NTA resin (Thermo Fisher Scientific) to remove the 6×His tag ...
-
bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
-
bioRxiv - Molecular Biology 2020Quote: The lysate and purified proteins were incubated with 3 µg of biotinylated RNA probe coupled to 20 µl Dynabeads MyOne Streptavidin C1 (Thermo Fisher) in 600 µl lysis buffer at 4°C for 1 hour rotating head-over-tail ...
-
bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
-
bioRxiv - Neuroscience 2021Quote: Flash frozen samples (n=3 per genotype) were prepared for Western blot using the Mem-PER Plus protein extraction kit (89842, Thermo Scientific) to isolate membrane and cytoplasmic protein fractions from mice aged P29-32 ...
-
bioRxiv - Cell Biology 2022Quote: ... was added to the supernatant and 100 μg of protein was loaded onto a 3-12% native gel (NativePAGE™ (Invitrogen)) ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were run on a commercial native- PAGE gel (3 to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well, Invitrogen, Cat. BN1001BOX) at 100 V tension using as running buffer 50 mM BisTris ...
-
bioRxiv - Systems Biology 2021Quote: ... coli phosphate-binding protein labeled with 7-Diethylamino-3-[N-(2-maleimidoethyl)carbamoyl]coumarin (MDCC) (phosphate sensor, CAT # PV4406, Thermo Fisher) upon binding of the free phosphate GTP hydrolysis product (excitation at 425 nm ...
-
bioRxiv - Immunology 2020Quote: Aag2 cells were seeded at 1 × 106 cells/well in 12-well plates and transfected at the time of seeding with 3 μg protein expression plasmid using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific) as per manufacturer’s instructions ...