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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... We lysed the red blood cells by resuspending the cell pellet in 3 mL of ACK lysing Buffer (Gibco, #A1049201) and incubated for 3 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... BAG2 or HSPA8 are transfected to U2OS cells at a concentration of 3 fmol/5,000 cells using Lipofectamine RNAiMAX Transfection Reagent (Thermofisher, 13778075) for three or four days before the experiment.
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bioRxiv - Genetics 2022Quote: The cloned SNP STARRseq library (100ug plasmid DNA/replica) was transiently transfected into LNCaP cells (5 × 107 cells/replica; 3 biological replicas) using the Neon Transfection System (Invitrogen). Cells were grown in RPMI 1640 medium supplemented with 10% FBS and collected 48hrs post electroporation ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected into HEK293T cells (3×105/well) using PEI or into CHO cells (1.5×105/well) with lipofectamine 3000 reagent (ThermoFisher, L3000001) in 24-well plates ...
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bioRxiv - Cell Biology 2020Quote: ... FACS analysis of transfected BEAS-2B cells for caspase 3 & caspase 7 along with SYTOX AADvancedTM Dead Cell Stain was performed per manufacturer’s protocol (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... 2×108 spleen cells and inguinal lymph node cells (about 1×108 B cells) were mixed with 108 Sp2/0 cells and washed 3 times with RPMI1640 medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The Gibson reaction mix was transformed into Mach1 competent cells (4 μl Gibson into 40 μl cells, 288 reactions in 3×96-well plates) following manufacture’s protocol (Invitrogen C862003). After transformation ...
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: ... The cells were dissociated with a 20µL pipette tip and resuspended in 1mL medium followed by dilution 1:2 with 0.4% trypan blue and loading of 10µL to a Countess cell culture chamber slide and quantification on a Countess 3 cell counter (Thermo Scientific).
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bioRxiv - Immunology 2022Quote: ... We then lysed the red blood cells (RBCs) by resuspending the cell pellet in 3 ml of ACK Lysing Buffer (Invitrogen) and incubated for 3 min at RT ...
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bioRxiv - Genomics 2022Quote: ... and measuring the fraction of sgRNA-expressing cells 3-12 days after transduction as mCherry-positive cells by flow cytometry on an Attune NxT (ThermoFisher). All experiments were performed in duplicates from the infection step.
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bioRxiv - Molecular Biology 2022Quote: Drosophila S2 cells were seeded at 3 X 106 cells in 3ml of Schneider’s Drosophila Medium (Life Technologies, Waltham, MA) supplemented with 10% fetal bovine serum (VWR ...
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bioRxiv - Biophysics 2024Quote: ... the cells were passaged by detaching the cells with 3 [mL] of TrypLE Express (with Phenol Red, 1X, Gibco, ThermoFisher) for 2 [min] and then plating 1,5×106cells/[mL] in a new coated Corning culture dishes with 100 [mm] diameter in a total of 10 [mL] new full endothelial growth medium.
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bioRxiv - Biophysics 2024Quote: ... the cells were passaged by detaching the cells with 3 [mL] of TrypLE Express (with Phenol Red, 1X, Gibco, ThermoFisher) for 2 [min] and then plating 1,5×106cells/[mL] in a new coated Corning culture dishes with 100 [mm] diameter in a total of 10 [mL] new full endothelial growth medium.
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bioRxiv - Molecular Biology 2022Quote: 4 L exponentially growing Sf9 cells at a density of 2-3 × 106 cells/mL in Sf900-III SFM media (Invitrogen) was infected with the high-titer baculovirus stock ...
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bioRxiv - Microbiology 2023Quote: ... suspension-adapted and serum-free HEK293S cells were seeded at 3 × 106 cells/mL in Freestyle-293 medium (ThermoFisher Scientific). Next ...
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was assessed at different time points (3, 5, 7 and 9 days) using AlamarBlue Cell Viability Reagent (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Cell line was maintained in culture at a density of 3×105 cells/ml in RPMI medium (Gibco-Life Technologies) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2024Quote: ... Cell line was maintained in culture at a density of 3×105 cells/ml in RPMI medium (Gibco-Life Technologies) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Synthetic Biology 2023Quote: ... LentiX HEK293T and 143b osteosarcoma cells adherent cell lines were passaged every 2-3 days by dissociating in 0.05% trypsin (Life Technologies) for 5 min at 37 °C or 1x DPBS supplemented with 2% FBS and 5 mM EDTA for 10 - 15 min at 37 °C to remove from culture plates ...
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bioRxiv - Systems Biology 2023Quote: ... The cell viability was evaluated using the Trypan Blue Assay (Strober, 2019) and the Countess 3 FL Automated Cell Counter (Invitrogen™ ...
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bioRxiv - Bioengineering 2024Quote: HER2+ K562 and SK-BR-3 cells were isolated from the cell culture supernatant or via dissociation with TrypLE (Gibco), respectively ...
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bioRxiv - Cell Biology 2020Quote: Protein concentration of cell lysates was determined using the Pierce™ BCA protein assay kit according to manufacturer’s instructions (ThermoFisher Scientific). Samples were subsequently subjected to SDS-PAGE and blotted on a nitrocellulose membrane ...
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bioRxiv - Developmental Biology 2020Quote: Whole-cell protein extracts were isolated from human cells with conventional RIPA lysis buffer and protein concentration assayed by BCA kit (Thermo Scientific). Blocking was carried out in 10% skim milk in PBST for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was incubated with superparamagnetic beads covalently linked to recombinant protein G (Dynabeads protein G Novex, Life technologies, USA) to obtain the precleared lysate ...
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bioRxiv - Cell Biology 2019Quote: ... The concentration of protein on beads and whole cell extracts were measured using the BCA assay (Pierce BCA protein assay kit, ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were centrifuged at 4 °C for 10 minutes at 16000 g to remove cell debris and protein concentration of the lysate was measured using Pierce BCA Protein Assay Kit (Thermo Scientific). Equal amounts (10 µg ...
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bioRxiv - Microbiology 2021Quote: ... Conditional supernatants from lentivirus-infected CHO cells sorted by FACS were used to purify the FXa-Fc fusion protein using a protein G column (89927, Thermo Fisher). For in vivo testing ...
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bioRxiv - Cell Biology 2022Quote: Protein concentration of control and stimulated myotube whole cell lysates was determined using Pierce™ BCA protein assay kit (ThermoFisher Scientific) following the manufacturer’s instructions as described before [37] ...
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bioRxiv - Pathology 2022Quote: ... Cell lysates were centrifuged and the protein concentration of the supernatants were quantitated using BCA protein assay kit (Thermo Scientific, 23227). Cell extracts (800 μg ...
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bioRxiv - Cell Biology 2022Quote: For extraction of membrane and cytosol proteins the cells were lysed according to the Mem-PER Plus Membrane Protein Kit protocol (#89842, ThermoFisher Scientific). The supernatants were kept frozen in −80 °C until total protein quantification ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysed cell suspensions were quantified for protein concentration using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Loughborough, UK). Working solutions were made up to 0.5 μg/μl with RIPA and 2x Concentrate Laemmli Sample Buffer (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... subcellular protein fractionation was performed as described in the Subcellular protein fractionation kit for cultured cells (Thermo Scientific; Cat. No. 78840). Fractions were stored in −80°C per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: Protein concentration in the cell extract was measured using a Pierce BCA protein assay kit (product 23227; Thermo Scientific, Rockford, IL).
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bioRxiv - Animal Behavior and Cognition 2021Quote: Total proteins were isolated from cells and tissues using the Nuclear and Cytoplasmic Protein Extraction Kit (Thermo Fisher Scientific, Waltham, USA). Approximately 40 μg of protein was separated on 12% SDS-polyacrylamide gel (SDS-PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein amounts of the OM cell lysates were quantified with BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein concentration of the cell lysates was then determined using Pierce™ BCA Protein Assay Kit from ThermoFisher (#23225). After 100 mM DTT was added ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein contents of total cell lysates were determined using a BCA protein assay kit (Thermo Scientific Pierce, Rockford, IL, USA). The equal amounts of protein were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Genomics 2023Quote: ... Eluted proteins and 50 μg of protein from whole cell lysate were resolved in 4-15% NuPAGE gradient gel (ThermoFisher, NP0335) using MOPS running buffer (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: Total proteins from intestinal epithelial cells or intestinal organoids were extracted with M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) with proteinase inhibitor (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... For normalization total protein concentration was measured on an aliquot of the cell lysate with the Pierce BCA protein kit (Thermo Fisher) following the manufacturer’s instructions ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... A small aliquot of cell lysate was later thawed and used for total protein quantification following the “BCA Protein Assay Kit” manual (Thermo Scientific). The same quantity (60 μL ...
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bioRxiv - Molecular Biology 2024Quote: Protein was isolated from cells as described (Rüegsegger et al., 2001) and quantified by BCA protein assay (#23225, Thermo Fisher Scientific). 40 µg of protein was separated on 10% surePAGE Bis-Tris gels in MOPS running buUer (#M00665 ...
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bioRxiv - Developmental Biology 2022Quote: ... 3% FBS (Gibco), 1% MEM non-essential amino acids (Gibco) ...
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bioRxiv - Genomics 2019Quote: ... 3 (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: ... DiOC2(3) (Invitrogen) was added to a final concentration of 30µM or DiSC3(5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% FBS (Invitrogen), 20 ng ml−1 EGF (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO2/3 (Invitrogen), β-catenin (BD Transduction Laboratories) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 (Applied Biosystems). We assembled forward and reverse reads using the Geneious (https://www.geneious.com ...
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bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Gibco), 0.1mM MEM-NEAA (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... TOPRO-3 (Invitrogen) was added to the staining buffer at a dilution of 1:10000 ...